Rorug03G0356500

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000286
Physical Location & Seq
Forward (+)
44225341 .. 44225952
612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0356500.1

Sequence Viewer

Length: 261 bp
ATGGTGCGCCAAGCTGTCGAAGACGCCAAACAGAGGGGGCAGGTGGCAGTCAGAGCTGAAGTTGAGAAATGGCTGACCAGCGTGGACAGGCTCACCGAAGAGGTGACCAAATTCTTCGAGTACAAAGGTGATGCCAATGACGGCTGGTTTCGTTGGTTGTATGGCTTGTTGATGTCGCGATACCAATTGAGCAAGAAGGCAAACAAGATGATAGAGCATCTTGTTCAACTCCAAGCTGATGGAGCCTTTCTAATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

86

Amino Acids

10.07

Weight (kDa)

8.01

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 31
AccII CGCG 1 cut(s) 178
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 1 cut(s) 33
AgsI TTSAA 1 cut(s) 227
AluBI AGCT 3 cut(s) 14, 56, 236
AluI AGCT 3 cut(s) 14, 56, 236
ApoI RAATTY 1 cut(s) 110
AseI ATTAAT 1 cut(s) 255
AspLEI GCGC 1 cut(s) 9
AsuHPI GGTGA 3 cut(s) 85, 115, 140
BbsI GAAGAC 1 cut(s) 27
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 157
BfuAI ACCTGC 1 cut(s) 31
BmiI GGNNCC 1 cut(s) 244
BmsI GCATC 2 cut(s) 121, 226
BpiI GAAGAC 1 cut(s) 27
BsaHI GRCGYC 1 cut(s) 24
Bsc4I CCNNNNNNNGG 1 cut(s) 33
BseLI CCNNNNNNNGG 1 cut(s) 33
Bsh1236I CGCG 1 cut(s) 178
BslI CCNNNNNNNGG 1 cut(s) 33
Bsp68I TCGCGA 1 cut(s) 178
BspFNI CGCG 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 244
BspMI ACCTGC 1 cut(s) 31
BssNI GRCGYC 1 cut(s) 24
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 24
BstEII GGTNACC 1 cut(s) 103
BstFNI CGCG 1 cut(s) 178
BstHHI GCGC 1 cut(s) 9
BstMWI GCNNNNNNNGC 2 cut(s) 53, 242
BstPI GGTNACC 1 cut(s) 103
BstUI CGCG 1 cut(s) 178
BstV2I GAAGAC 1 cut(s) 27
BstXI CCANNNNNNTGG 1 cut(s) 239
BtuMI TCGCGA 1 cut(s) 178
BveI ACCTGC 1 cut(s) 31
CfoI GCGC 1 cut(s) 9
CseI GACGC 1 cut(s) 32
Csp6I GTAC 1 cut(s) 121
CviJI RGCY 8 cut(s) 14, 56, 73, 91, 144, 165, 236, 245
CviKI_1 RGCY 8 cut(s) 14, 56, 73, 91, 144, 165, 236, 245
CviQI GTAC 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Eco57I CTGAAG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 103
EcoO65I GGTNACC 1 cut(s) 103
FaiI YATR 1 cut(s) 162
GlaI GCGC 1 cut(s) 8
HgaI GACGC 1 cut(s) 32
HhaI GCGC 1 cut(s) 9
Hin1I GRCGYC 1 cut(s) 24
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HphI GGTGA 3 cut(s) 85, 115, 140
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 1 cut(s) 53
Hpy188III TCNNGA 1 cut(s) 177
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 1 cut(s) 190
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 242
Hsp92I GRCGYC 1 cut(s) 24
HspAI GCGC 1 cut(s) 7
LmnI GCTCC 1 cut(s) 242
LpnPI CCDG 4 cut(s) 26, 73, 91, 130
LweI GCATC 2 cut(s) 121, 226
MaeIII GTNAC 1 cut(s) 103
MboII GAAGA 3 cut(s) 32, 106, 110
MfeI CAATTG 1 cut(s) 185
MluCI AATT 4 cut(s) 110, 185, 252, 256
MnlI CCTC 2 cut(s) 27, 94
MseI TTAA 1 cut(s) 255
MunI CAATTG 1 cut(s) 185
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 2 cut(s) 53, 242
NlaIV GGNNCC 1 cut(s) 244
NmuCI GTSAC 1 cut(s) 103
NruI TCGCGA 1 cut(s) 178
PaqCI CACCTGC 1 cut(s) 31
PshBI ATTAAT 1 cut(s) 255
PspEI GGTNACC 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 244
RruI TCGCGA 1 cut(s) 178
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SaqAI TTAA 1 cut(s) 255
SetI ASST 6 cut(s) 16, 45, 58, 105, 130, 238
SfaNI GCATC 2 cut(s) 121, 226
Sse9I AATT 4 cut(s) 110, 185, 252, 256
TaqI TCGA 2 cut(s) 18, 117
TasI AATT 4 cut(s) 110, 185, 252, 256
TatI WGTACW 1 cut(s) 120
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TseFI GTSAC 1 cut(s) 103
Tsp45I GTSAC 1 cut(s) 103
VspI ATTAAT 1 cut(s) 255
XapI RAATTY 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.