Rh4BG061400

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000286
Physical Location & Seq
Forward (+)
11023424 .. 11023919
496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG061400.1

Sequence Viewer

Length: 312 bp
ATGGACATGATTACTCTTCTCTACATACTTTATAGTGGGAGACCATTCTGTAATGGAGACTGCAAAAACTTCATTAAGGGAAGTTATTTCACTTGCGTCAAATGCTATGATAATTCCACCGATACATTCAACGTTTGCGCGGCCTGTTATCTTGACGCCAAGTATGTCCACCAACACGAAAAATTGCTTGATAACTGTCTTCTGCCAGAAGCCAAAAGGAGGGAAACTCTGAGCTTGAAGCTGCAGCAAGAAGCATCAAGCATAATTAATGAGGTAAATAATAAATCGGTGCATCTGTTCATCATTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5

Protein Analysis

103

Amino Acids

11.85

Weight (kDa)

6.79

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 140
AciI CCGC 1 cut(s) 140
AclI AACGTT 1 cut(s) 132
AcyI GRCGYC 1 cut(s) 156
AfiI CCNNNNNNNGG 1 cut(s) 219
AgsI TTSAA 2 cut(s) 130, 238
AluBI AGCT 2 cut(s) 234, 241
AluI AGCT 2 cut(s) 234, 241
Alw26I GTCTC 2 cut(s) 34, 51
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 241, 244
AseI ATTAAT 1 cut(s) 267
AspLEI GCGC 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 191
BbvI GCAGC 2 cut(s) 228, 256
BcoDI GTCTC 2 cut(s) 34, 51
BfmI CTRYAG 1 cut(s) 242
BisI GCNGC 3 cut(s) 141, 242, 245
BlsI GCNGC 3 cut(s) 142, 243, 246
BmsI GCATC 2 cut(s) 263, 301
BpiI GAAGAC 1 cut(s) 191
BplI GAGNNNNNCTC 2 cut(s) 211, 243
BsaHI GRCGYC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 1 cut(s) 219
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMII CTCAG 1 cut(s) 221
BseXI GCAGC 2 cut(s) 228, 256
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 2 cut(s) 34, 51
BsnI GGCC 1 cut(s) 143
Bso31I GGTCTC 1 cut(s) 34
BspACI CCGC 1 cut(s) 140
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 1 cut(s) 222
BspFNI CGCG 1 cut(s) 140
BspMAI CTGCAG 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 34
BssNI GRCGYC 1 cut(s) 156
Bst4CI ACNGT 1 cut(s) 197
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 156
BstDEI CTNAG 1 cut(s) 230
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 140
BstMAI GTCTC 2 cut(s) 34, 51
BstMWI GCNNNNNNNGC 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 242
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 2 cut(s) 228, 256
BstV2I GAAGAC 1 cut(s) 191
BsuRI GGCC 1 cut(s) 143
CfoI GCGC 1 cut(s) 140
CseI GACGC 2 cut(s) 85, 164
CviAII CATG 1 cut(s) 7
CviJI RGCY 4 cut(s) 143, 212, 234, 241
CviKI_1 RGCY 4 cut(s) 143, 212, 234, 241
DdeI CTNAG 1 cut(s) 230
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 34
FaeI CATG 1 cut(s) 10
FaiI YATR 6 cut(s) 8, 26, 33, 108, 165, 263
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 3 cut(s) 141, 242, 245
Fsp4HI GCNGC 3 cut(s) 141, 242, 245
GlaI GCGC 1 cut(s) 139
GluI GCNGC 3 cut(s) 141, 242, 245
HaeIII GGCC 1 cut(s) 143
HgaI GACGC 2 cut(s) 85, 164
HhaI GCGC 1 cut(s) 140
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 1 cut(s) 10
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 1 cut(s) 231
Hpy188III TCNNGA 1 cut(s) 152
Hpy8I GTNNAC 1 cut(s) 169
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 3 cut(s) 63, 244, 292
HpyF10VI GCNNNNNNNGC 1 cut(s) 102
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 1 cut(s) 10
HspAI GCGC 1 cut(s) 138
LpnPI CCDG 2 cut(s) 157, 219
Lsp1109I GCAGC 2 cut(s) 228, 256
LweI GCATC 2 cut(s) 263, 301
MaeII ACGT 1 cut(s) 132
MboII GAAGA 2 cut(s) 8, 191
MluCI AATT 3 cut(s) 112, 182, 264
MnlI CCTC 2 cut(s) 213, 265
MseI TTAA 2 cut(s) 75, 267
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 1 cut(s) 102
NlaIII CATG 1 cut(s) 10
PkrI GCNGC 3 cut(s) 142, 243, 246
PshBI ATTAAT 1 cut(s) 267
Psp1406I AACGTT 1 cut(s) 132
PstI CTGCAG 1 cut(s) 246
SaqAI TTAA 2 cut(s) 75, 267
SatI GCNGC 3 cut(s) 141, 242, 245
SetI ASST 4 cut(s) 135, 236, 243, 276
SfaNI GCATC 2 cut(s) 263, 301
SfcI CTRYAG 1 cut(s) 242
Sse9I AATT 3 cut(s) 112, 182, 264
SsiI CCGC 1 cut(s) 140
TaaI ACNGT 1 cut(s) 197
TaiI ACGT 1 cut(s) 135
TasI AATT 3 cut(s) 112, 182, 264
TauI GCSGC 1 cut(s) 143
Tru1I TTAA 2 cut(s) 75, 267
Tru9I TTAA 2 cut(s) 75, 267
TseI GCWGC 2 cut(s) 241, 244
TspDTI ATGAA 2 cut(s) 61, 289
VspI ATTAAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.