Rmu_sc0005431.1_g000020

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005431.1
OG0000286
Physical Location & Seq
Reverse (-)
80187 .. 82010
1824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005431.1_g000020.1.cds

Sequence Viewer

Length: 1149 bp
atggaagaaatacgtgaagttgctttagcatactatgacaattgcacaccagaacttcggggtaagacatgggagttctttcaatccatggatacgaacggcgatcaccgaatcagtcccagggagttcaatgaattcctccgtcagaccggttacaacacgatcattgctgacccagaattatttgcaaagcttgaccgcaacggtgactccgggctggatttcgatgaagtactcactttctactatatcattaaaactgggtacgtctggtgccgaggctgcaacacgtacctttccgggctctattttacgtgcttcgaatgctttgaggcagccaatcactccacttttgatctatgtgatgcttgtcatcgcagtcagaagtttactcaccaccacacactcttcttggagaaccacatactgctatactacaaaagaggaccagatgacagcatggcaatggaatatctatatgcagaagctttagagtactataataaagcaactccagagcttcagagttcagtggtgtctttctttcaatccatggttacacacggtgcaaaccaaatcagcctctctgtgttcaacgatttcctcaagcaaaaaggctacaactggattattaatgatccactcttgttccagaaactcgaccgcaaccgtgatggctgcctcgatttctgggaattgctcaccttctactacatcatcagcacaaggtacttccattgccgagaatgtcaggtgtaccttggcgggctgtattttacctgcgttgcttgctttgagggaggagctcattatcgtcgtcatcatcattctgatctcggcacctataatctatgtgctgcctgctatcgcagtaggaatttcctctctcaacacccgcaccacaccttcttcttggataaccacatattgctgcgctccaaaaggggtcttcctccttatgctacaccaagtctggtaagcgctccaaaacctctggtggtttatagtcattattattttattaataataattaccaccttccagctgagcagcctgtacctccaaagaagagttggctatcgtcactcaacctattagaggtggccctggctggtgcaaacttggctgctaattgttcgattatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

382

Amino Acids

44.49

Weight (kDa)

6.16

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 788
AccB1I GGYRCC 2 cut(s) 273, 839
AciI CCGC 4 cut(s) 199, 664, 765, 896
AclWI GGATC 1 cut(s) 632
AcsI RAATTY 2 cut(s) 134, 877
AcuI CTGAAG 1 cut(s) 506
AdeI CACNNNGTG 1 cut(s) 566
AfaI GTAC 7 cut(s) 234, 266, 293, 497, 731, 758, 1059
AfeI AGCGCT 1 cut(s) 982
AfiI CCNNNNNNNGG 1 cut(s) 1099
AflIII ACRYGT 1 cut(s) 288
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 4 cut(s) 83, 130, 548, 595
AjnI CCWGG 2 cut(s) 119, 1107
AluBI AGCT 5 cut(s) 193, 488, 520, 806, 1046
AluI AGCT 5 cut(s) 193, 488, 520, 806, 1046
Alw21I GWGCWC 1 cut(s) 808
AlwI GGATC 1 cut(s) 632
Aor51HI AGCGCT 1 cut(s) 982
AoxI GGCC 1 cut(s) 1104
ApeKI GCWGC 7 cut(s) 282, 335, 678, 857, 931, 1051, 1127
ApoI RAATTY 2 cut(s) 134, 877
AseI ATTAAT 2 cut(s) 633, 1023
AsiGI ACCGGT 1 cut(s) 149
AspLEI GCGC 2 cut(s) 936, 983
AspS9I GGNCC 2 cut(s) 446, 1105
AsuC2I CCSGG 2 cut(s) 214, 301
AsuHPI GGTGA 4 cut(s) 98, 218, 386, 694
AsuII TTCGAA 1 cut(s) 321
AvaII GGWCC 1 cut(s) 446
BanI GGYRCC 2 cut(s) 273, 839
BanII GRGCYC 2 cut(s) 306, 808
BbsI GAAGAC 1 cut(s) 941
Bbv12I GWGCWC 1 cut(s) 808
BbvI GCAGC 7 cut(s) 269, 347, 665, 844, 918, 1063, 1114
BccI CCATC 1 cut(s) 668
BceAI ACGGC 1 cut(s) 115
BciT130I CCWGG 2 cut(s) 121, 1109
BciVI GTATCC 1 cut(s) 85
BcnI CCSGG 2 cut(s) 214, 301
BfoI RGCGCY 1 cut(s) 984
BfuAI ACCTGC 1 cut(s) 788
BfuI GTATCC 1 cut(s) 85
BisI GCNGC 7 cut(s) 283, 336, 679, 858, 932, 1052, 1128
BlpI GCTNAGC 1 cut(s) 1047
BlsI GCNGC 7 cut(s) 284, 337, 680, 859, 933, 1053, 1129
BmcAI AGTACT 2 cut(s) 234, 497
Bme1390I CCNGG 4 cut(s) 121, 214, 301, 1109
Bme18I GGWCC 1 cut(s) 446
BmgT120I GGNCC 2 cut(s) 446, 1105
BmiI GGNNCC 2 cut(s) 275, 841
BmrFI CCNGG 4 cut(s) 121, 214, 301, 1109
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 355
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 941
BpmI CTGGAG 1 cut(s) 498
Bpu1102I GCTNAGC 1 cut(s) 1047
Bpu14I TTCGAA 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 590
BpuMI CCSGG 2 cut(s) 214, 301
BsaAI YACGTR 3 cut(s) 14, 291, 315
BsaJI CCNNGG 7 cut(s) 87, 119, 120, 277, 552, 760, 1107
BsaWI WCCGGW 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 1099
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 2 cut(s) 265, 629
Bse3DI GCAATG 3 cut(s) 165, 471, 736
BseBI CCWGG 2 cut(s) 121, 1109
BseDI CCNNGG 7 cut(s) 87, 119, 120, 277, 552, 760, 1107
BseLI CCNNNNNNNGG 1 cut(s) 1099
BseMI GCAATG 3 cut(s) 165, 471, 736
BseMII CTCAG 1 cut(s) 1038
BseNI ACTGG 2 cut(s) 265, 629
BseRI GAGGAG 1 cut(s) 816
BseXI GCAGC 7 cut(s) 269, 347, 665, 844, 918, 1063, 1114
Bsh1285I CGRYCG 1 cut(s) 664
BshFI GGCC 1 cut(s) 1106
BshNI GGYRCC 2 cut(s) 273, 839
BshTI ACCGGT 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 664
BsiHKAI GWGCWC 1 cut(s) 808
BsiSI CCGG 3 cut(s) 150, 213, 300
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 1099
BsmFI GGGAC 1 cut(s) 102
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 1 cut(s) 1106
Bsp119I TTCGAA 1 cut(s) 321
Bsp1286I GDGCHC 2 cut(s) 306, 808
Bsp143I GATC 5 cut(s) 103, 162, 355, 637, 832
Bsp1720I GCTNAGC 1 cut(s) 1047
Bsp19I CCATGG 2 cut(s) 87, 552
BspACI CCGC 4 cut(s) 199, 664, 765, 896
BspANI GGCC 1 cut(s) 1106
BspCNI CTCAG 1 cut(s) 1039
BspLI GGNNCC 2 cut(s) 275, 841
BspMI ACCTGC 1 cut(s) 788
BspPI GGATC 1 cut(s) 632
BspT104I TTCGAA 1 cut(s) 321
BspT107I GGYRCC 2 cut(s) 273, 839
BsrDI GCAATG 3 cut(s) 165, 471, 736
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 2 cut(s) 265, 629
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 7 cut(s) 87, 119, 120, 277, 552, 760, 1107
BssMI GATC 5 cut(s) 103, 162, 355, 637, 832
BssT1I CCWWGG 3 cut(s) 87, 552, 760
Bst2UI CCWGG 2 cut(s) 121, 1109
Bst4CI ACNGT 3 cut(s) 206, 566, 671
Bst6I CTCTTC 2 cut(s) 413, 1064
BstBAI YACGTR 3 cut(s) 14, 291, 315
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 3 cut(s) 767, 790, 862
BstDEI CTNAG 1 cut(s) 1047
BstDSI CCRYGG 2 cut(s) 87, 552
BstENI CCTNNNNNAGG 1 cut(s) 1097
BstH2I RGCGCY 1 cut(s) 984
BstHHI GCGC 2 cut(s) 936, 983
BstKTI GATC 5 cut(s) 106, 165, 358, 640, 835
BstMBI GATC 5 cut(s) 103, 162, 355, 637, 832
BstMCI CGRYCG 1 cut(s) 664
BstMWI GCNNNNNNNGC 4 cut(s) 282, 324, 789, 1124
BstNI CCWGG 2 cut(s) 121, 1109
BstSCI CCNGG 4 cut(s) 119, 212, 299, 1107
BstV1I GCAGC 7 cut(s) 269, 347, 665, 844, 918, 1063, 1114
BstV2I GAAGAC 1 cut(s) 941
BsuI GTATCC 1 cut(s) 85
BsuRI GGCC 1 cut(s) 1106
BtgI CCRYGG 2 cut(s) 87, 552
BtgZI GCGATG 1 cut(s) 359
BtsIMutI CAGTG 1 cut(s) 537
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 3 cut(s) 767, 790, 862
CfoI GCGC 2 cut(s) 936, 983
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 446, 1105
Csp6I GTAC 7 cut(s) 233, 265, 292, 496, 730, 757, 1058
CspAI ACCGGT 1 cut(s) 149
CviAII CATG 4 cut(s) 69, 88, 460, 553
CviQI GTAC 7 cut(s) 233, 265, 292, 496, 730, 757, 1058
DdeI CTNAG 1 cut(s) 1047
DpnI GATC 5 cut(s) 105, 164, 357, 639, 834
DpnII GATC 5 cut(s) 103, 162, 355, 637, 832
DraIII CACNNNGTG 1 cut(s) 566
Eam1104I CTCTTC 2 cut(s) 413, 1064
EarI CTCTTC 2 cut(s) 413, 1064
Ecl136II GAGCTC 1 cut(s) 806
Eco130I CCWWGG 3 cut(s) 87, 552, 760
Eco24I GRGCYC 2 cut(s) 306, 808
Eco47I GGWCC 1 cut(s) 446
Eco47III AGCGCT 1 cut(s) 982
Eco53kI GAGCTC 1 cut(s) 806
Eco57I CTGAAG 1 cut(s) 506
EcoICRI GAGCTC 1 cut(s) 806
EcoNI CCTNNNNNAGG 1 cut(s) 1097
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 119, 1107
EcoT14I CCWWGG 3 cut(s) 87, 552, 760
EcoT38I GRGCYC 2 cut(s) 306, 808
ErhI CCWWGG 3 cut(s) 87, 552, 760
FaeI CATG 4 cut(s) 72, 91, 463, 556
FaqI GGGAC 1 cut(s) 102
FatI CATG 4 cut(s) 68, 87, 459, 552
FauI CCCGC 2 cut(s) 758, 903
Fnu4HI GCNGC 7 cut(s) 283, 336, 679, 858, 932, 1052, 1128
FriOI GRGCYC 2 cut(s) 306, 808
Fsp4HI GCNGC 7 cut(s) 283, 336, 679, 858, 932, 1052, 1128
GlaI GCGC 2 cut(s) 935, 982
GluI GCNGC 7 cut(s) 283, 336, 679, 858, 932, 1052, 1128
GsuI CTGGAG 1 cut(s) 498
HaeII RGCGCY 1 cut(s) 984
HaeIII GGCC 1 cut(s) 1106
HapII CCGG 3 cut(s) 150, 213, 300
HhaI GCGC 2 cut(s) 936, 983
Hin1II CATG 4 cut(s) 72, 91, 463, 556
Hin6I GCGC 2 cut(s) 934, 981
HinP1I GCGC 2 cut(s) 934, 981
HindIII AAGCTT 2 cut(s) 191, 486
HinfI GANTC 2 cut(s) 111, 209
HpaII CCGG 3 cut(s) 150, 213, 300
HphI GGTGA 4 cut(s) 98, 218, 386, 694
Hpy166II GTNNAC 2 cut(s) 390, 757
Hpy188I TCNGA 4 cut(s) 147, 384, 525, 832
Hpy188III TCNNGA 2 cut(s) 515, 652
Hpy8I GTNNAC 2 cut(s) 390, 757
Hpy99I CGWCG 1 cut(s) 819
HpyAV CCTTC 3 cut(s) 715, 916, 1049
HpyCH4III ACNGT 3 cut(s) 206, 566, 671
HpyCH4IV ACGT 4 cut(s) 13, 267, 290, 314
HpyCH4V TGCA 6 cut(s) 45, 188, 285, 482, 569, 1118
HpyF10VI GCNNNNNNNGC 4 cut(s) 282, 324, 789, 1124
HpyF3I CTNAG 1 cut(s) 1047
HpySE526I ACGT 4 cut(s) 13, 267, 290, 314
Hsp92II CATG 4 cut(s) 72, 91, 463, 556
HspAI GCGC 2 cut(s) 934, 981
Kzo9I GATC 5 cut(s) 103, 162, 355, 637, 832
LmnI GCTCC 3 cut(s) 803, 941, 988
Lsp1109I GCAGC 7 cut(s) 269, 347, 665, 844, 918, 1063, 1114
LweI GCATC 1 cut(s) 355
MaeII ACGT 4 cut(s) 13, 267, 290, 314
MaeIII GTNAC 4 cut(s) 152, 206, 556, 1083
MalI GATC 5 cut(s) 105, 164, 357, 639, 834
MboI GATC 5 cut(s) 103, 162, 355, 637, 832
MboII GAAGA 5 cut(s) 17, 400, 901, 941, 1081
MfeI CAATTG 1 cut(s) 40
MhlI GDGCHC 2 cut(s) 306, 808
MluCI AATT 7 cut(s) 40, 134, 179, 695, 877, 1030, 1132
MlyI GAGTC 1 cut(s) 203
MseI TTAA 3 cut(s) 255, 633, 1023
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 1046
MspI CCGG 3 cut(s) 150, 213, 300
MspR9I CCNGG 4 cut(s) 121, 214, 301, 1109
MunI CAATTG 1 cut(s) 40
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 2 cut(s) 121, 1109
MwoI GCNNNNNNNGC 4 cut(s) 282, 324, 789, 1124
NciI CCSGG 2 cut(s) 214, 301
NcoI CCATGG 2 cut(s) 87, 552
NdeII GATC 5 cut(s) 103, 162, 355, 637, 832
NlaIII CATG 4 cut(s) 72, 91, 463, 556
NlaIV GGNNCC 2 cut(s) 275, 841
NmeAIII GCCGAG 3 cut(s) 302, 767, 816
NmuCI GTSAC 2 cut(s) 206, 1083
NspV TTCGAA 1 cut(s) 321
PasI CCCWGGG 1 cut(s) 120
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 111
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 7 cut(s) 284, 337, 680, 859, 933, 1053, 1129
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
Ppu21I YACGTR 3 cut(s) 14, 291, 315
PshBI ATTAAT 2 cut(s) 633, 1023
Psp124BI GAGCTC 1 cut(s) 808
Psp6I CCWGG 2 cut(s) 119, 1107
PspGI CCWGG 2 cut(s) 119, 1107
PspN4I GGNNCC 2 cut(s) 275, 841
PspPI GGNCC 2 cut(s) 446, 1105
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 7 cut(s) 234, 266, 293, 497, 731, 758, 1059
RsaNI GTAC 7 cut(s) 233, 265, 292, 496, 730, 757, 1058
RseI CAYNNNNRTG 1 cut(s) 464
SacI GAGCTC 1 cut(s) 808
SaqAI TTAA 3 cut(s) 255, 633, 1023
SatI GCNGC 7 cut(s) 283, 336, 679, 858, 932, 1052, 1128
Sau3AI GATC 5 cut(s) 103, 162, 355, 637, 832
Sau96I GGNCC 2 cut(s) 446, 1105
ScaI AGTACT 2 cut(s) 234, 497
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 4 cut(s) 121, 214, 301, 1109
SduI GDGCHC 2 cut(s) 306, 808
SfaNI GCATC 1 cut(s) 355
SfuI TTCGAA 1 cut(s) 321
SinI GGWCC 1 cut(s) 446
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
Sse9I AATT 7 cut(s) 40, 134, 179, 695, 877, 1030, 1132
SsiI CCGC 4 cut(s) 199, 664, 765, 896
SstI GAGCTC 1 cut(s) 808
StyD4I CCNGG 4 cut(s) 119, 212, 299, 1107
StyI CCWWGG 3 cut(s) 87, 552, 760
TaaI ACNGT 3 cut(s) 206, 566, 671
TaiI ACGT 4 cut(s) 16, 270, 293, 317
TaqI TCGA 5 cut(s) 225, 321, 660, 684, 1139
TasI AATT 7 cut(s) 40, 134, 179, 695, 877, 1030, 1132
TatI WGTACW 2 cut(s) 232, 495
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 3 cut(s) 255, 633, 1023
Tru9I TTAA 3 cut(s) 255, 633, 1023
TscAI CASTG 1 cut(s) 537
TseFI GTSAC 2 cut(s) 206, 1083
TseI GCWGC 7 cut(s) 282, 335, 678, 857, 931, 1051, 1127
Tsp45I GTSAC 2 cut(s) 206, 1083
TspDTI ATGAA 2 cut(s) 147, 243
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 1 cut(s) 537
VpaK11BI GGWCC 1 cut(s) 446
VspI ATTAAT 2 cut(s) 633, 1023
XagI CCTNNNNNAGG 1 cut(s) 1097
XapI RAATTY 2 cut(s) 134, 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 1071
ZrmI AGTACT 2 cut(s) 234, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.