Rw0G006720

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00246
OG0000286
Physical Location & Seq
Forward (+)
617 .. 5978
5362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G006720.1

Sequence Viewer

Length: 495 bp
ATGCGGTCGGCAGTAGAACATTTTGTCCAGGAGATGGATCGTGATGCAAAGGACCGAGTGAGCCGGCAAGATTTCTTAGAATACATGAAGATGGATGAAGATTGTGCACATATGAATAACCACAGGTTCTTTGATGTGCTGAAGAAGGAAGAGAGCGAGGAGCTGGATTTCATGGACTCAAAGGCACATGAGTCCGTTGGAACAAAAGATCTGAAAAAGAAGCTCCAGTCTAGCAGTGGCAGTAGAACAGTGACTACTAGAGAAGCAAAAGCCTCTTCTTCTTCTCCTAAACCACCGACACCGGAGCCAAGCAGTTCCACATCCAATGCACCGGAGGGAAGTAATCGCTCCACATCCAACGCTCTGGTTCCCGTGAATCCTAAGCCTAAACATAGAAGAAGCGCAGGGAAGAAAGCAATTAAGACGGCGGAGCTGGCACTTGCCCTGGGAAATATATTTGTAACCAGTACTGCGTCTCAGTGTGCCATTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5

Protein Analysis

164

Amino Acids

18.1

Weight (kDa)

8.87

Isoelectric Point (pI)

61.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 34
AciI CCGC 2 cut(s) 4, 428
AclWI GGATC 1 cut(s) 45
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 1 cut(s) 469
AfiI CCNNNNNNNGG 1 cut(s) 34
AjnI CCWGG 2 cut(s) 27, 444
AloI GAACNNNNNNTCC 2 cut(s) 9, 41
AluBI AGCT 3 cut(s) 163, 223, 433
AluI AGCT 3 cut(s) 163, 223, 433
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 1 cut(s) 480
Alw44I GTGCAC 1 cut(s) 105
AlwI GGATC 1 cut(s) 45
ApaLI GTGCAC 1 cut(s) 105
AspLEI GCGC 1 cut(s) 404
AspS9I GGNCC 1 cut(s) 52
AvaII GGWCC 1 cut(s) 52
BaeGI GKGCMC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 109
BccI CCATC 2 cut(s) 28, 85
BceAI ACGGC 1 cut(s) 441
BciT130I CCWGG 2 cut(s) 29, 446
BcoDI GTCTC 1 cut(s) 480
BfaI CTAG 2 cut(s) 231, 258
BglII AGATCT 1 cut(s) 208
BmcAI AGTACT 1 cut(s) 469
Bme1390I CCNGG 2 cut(s) 29, 446
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 2 cut(s) 306, 369
BmrFI CCNGG 2 cut(s) 29, 446
BmsI GCATC 1 cut(s) 34
BpmI CTGGAG 1 cut(s) 209
Bpu10I CCTNAGC 1 cut(s) 381
BsaJI CCNNGG 2 cut(s) 444, 445
BsaWI WCCGGW 2 cut(s) 301, 331
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 1 cut(s) 63
Bse1I ACTGG 2 cut(s) 226, 465
BseBI CCWGG 2 cut(s) 29, 446
BseDI CCNNGG 2 cut(s) 444, 445
BseGI GGATG 3 cut(s) 100, 320, 353
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 491
BseNI ACTGG 2 cut(s) 226, 465
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 109
Bsh1285I CGRYCG 1 cut(s) 8
BsiEI CGRYCG 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 109
BsiSI CCGG 3 cut(s) 64, 302, 332
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 1 cut(s) 480
BsmBI CGTCTC 1 cut(s) 480
Bsp1286I GDGCHC 1 cut(s) 109
Bsp143I GATC 2 cut(s) 37, 208
BspACI CCGC 2 cut(s) 4, 428
BspCNI CTCAG 1 cut(s) 490
BspLI GGNNCC 2 cut(s) 306, 369
BspPI GGATC 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 63
BsrI ACTGG 2 cut(s) 226, 465
BssAI RCCGGY 1 cut(s) 63
BssECI CCNNGG 2 cut(s) 444, 445
BssMI GATC 2 cut(s) 37, 208
Bst2UI CCWGG 2 cut(s) 29, 446
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 2 cut(s) 144, 280
BstC8I GCNNGC 2 cut(s) 65, 435
BstDEI CTNAG 3 cut(s) 76, 381, 477
BstF5I GGATG 3 cut(s) 100, 320, 353
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 2 cut(s) 40, 211
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 2 cut(s) 37, 208
BstMCI CGRYCG 1 cut(s) 8
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNI CCWGG 2 cut(s) 29, 446
BstSCI CCNGG 2 cut(s) 27, 444
BstSLI GKGCMC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 208
BstXI CCANNNNNNTGG 1 cut(s) 364
BstYI RGATCY 1 cut(s) 208
BtsCI GGATG 3 cut(s) 100, 320, 353
BtsI GCAGTG 1 cut(s) 241
BtsIMutI CAGTG 3 cut(s) 241, 255, 485
Cac8I GCNNGC 2 cut(s) 65, 435
CfoI GCGC 1 cut(s) 404
Cfr10I RCCGGY 1 cut(s) 63
Cfr13I GGNCC 1 cut(s) 52
CseI GACGC 1 cut(s) 462
Csp6I GTAC 1 cut(s) 468
CviAII CATG 3 cut(s) 85, 172, 188
CviJI RGCY 7 cut(s) 63, 163, 223, 272, 307, 385, 433
CviKI_1 RGCY 7 cut(s) 63, 163, 223, 272, 307, 385, 433
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 3 cut(s) 76, 381, 477
DpnI GATC 2 cut(s) 39, 210
DpnII GATC 2 cut(s) 37, 208
Eam1104I CTCTTC 2 cut(s) 144, 280
EarI CTCTTC 2 cut(s) 144, 280
EciI GGCGGA 1 cut(s) 443
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 27, 444
Esp3I CGTCTC 1 cut(s) 480
FaeI CATG 3 cut(s) 88, 175, 191
FaiI YATR 7 cut(s) 86, 111, 113, 173, 189, 393, 455
FatI CATG 3 cut(s) 84, 171, 187
FauNDI CATATG 1 cut(s) 111
FokI GGATG 3 cut(s) 107, 307, 340
FspBI CTAG 2 cut(s) 231, 258
GlaI GCGC 1 cut(s) 403
GsuI CTGGAG 1 cut(s) 209
HapII CCGG 3 cut(s) 64, 302, 332
HgaI GACGC 1 cut(s) 462
HhaI GCGC 1 cut(s) 404
Hin1II CATG 3 cut(s) 88, 175, 191
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HinfI GANTC 3 cut(s) 176, 191, 376
HpaII CCGG 3 cut(s) 64, 302, 332
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 2 cut(s) 213, 494
Hpy188III TCNNGA 1 cut(s) 41
Hpy8I GTNNAC 1 cut(s) 107
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4V TGCA 3 cut(s) 47, 107, 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpyF3I CTNAG 3 cut(s) 76, 381, 477
Hsp92II CATG 3 cut(s) 88, 175, 191
HspAI GCGC 1 cut(s) 402
KroI GCCGGC 1 cut(s) 63
KroNI GCCGGC 1 cut(s) 65
Kzo9I GATC 2 cut(s) 37, 208
LmnI GCTCC 5 cut(s) 160, 228, 304, 353, 430
LweI GCATC 1 cut(s) 34
MaeI CTAG 2 cut(s) 231, 258
MaeIII GTNAC 2 cut(s) 250, 460
MalI GATC 2 cut(s) 39, 210
MboI GATC 2 cut(s) 37, 208
MboII GAAGA 9 cut(s) 100, 110, 154, 161, 267, 270, 273, 408, 421
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 109
MluCI AATT 1 cut(s) 417
MlyI GAGTC 2 cut(s) 170, 200
MmeI TCCRAC 2 cut(s) 178, 381
MnlI CCTC 3 cut(s) 151, 283, 328
MroNI GCCGGC 1 cut(s) 63
MseI TTAA 1 cut(s) 420
MslI CAYNNNNRTG 1 cut(s) 89
MspI CCGG 3 cut(s) 64, 302, 332
MspR9I CCNGG 2 cut(s) 29, 446
MvaI CCWGG 2 cut(s) 29, 446
MwoI GCNNNNNNNGC 1 cut(s) 434
NaeI GCCGGC 1 cut(s) 65
NdeI CATATG 1 cut(s) 111
NdeII GATC 2 cut(s) 37, 208
NgoMIV GCCGGC 1 cut(s) 63
NlaIII CATG 3 cut(s) 88, 175, 191
NlaIV GGNNCC 2 cut(s) 306, 369
NmuCI GTSAC 1 cut(s) 250
PasI CCCWGGG 1 cut(s) 445
PdiI GCCGGC 1 cut(s) 65
PfeI GAWTC 1 cut(s) 376
PflMI CCANNNNNTGG 1 cut(s) 34
PfoI TCCNGGA 1 cut(s) 27
PleI GAGTC 2 cut(s) 170, 199
PpsI GAGTC 2 cut(s) 170, 199
Psp6I CCWGG 2 cut(s) 27, 444
PspGI CCWGG 2 cut(s) 27, 444
PspN4I GGNNCC 2 cut(s) 306, 369
PspPI GGNCC 1 cut(s) 52
PsrI GAACNNNNNNTAC 2 cut(s) 238, 270
PsuI RGATCY 1 cut(s) 208
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
RseI CAYNNNNRTG 1 cut(s) 89
SaqAI TTAA 1 cut(s) 420
Sau3AI GATC 2 cut(s) 37, 208
Sau96I GGNCC 1 cut(s) 52
ScaI AGTACT 1 cut(s) 469
SchI GAGTC 2 cut(s) 170, 200
ScrFI CCNGG 2 cut(s) 29, 446
SduI GDGCHC 1 cut(s) 109
SetI ASST 4 cut(s) 128, 165, 225, 435
SfaNI GCATC 1 cut(s) 34
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 89
Sse9I AATT 1 cut(s) 417
SsiI CCGC 2 cut(s) 4, 428
SspMI CTAG 2 cut(s) 231, 258
StyD4I CCNGG 2 cut(s) 27, 444
TaaI ACNGT 1 cut(s) 250
TaqII GACCGA 1 cut(s) 69
TasI AATT 1 cut(s) 417
TatI WGTACW 1 cut(s) 467
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 3 cut(s) 241, 255, 485
TseFI GTSAC 1 cut(s) 250
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 4 cut(s) 101, 111, 128, 160
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 3 cut(s) 241, 255, 485
Van91I CCANNNNNTGG 1 cut(s) 34
VneI GTGCAC 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 52
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XspI CTAG 2 cut(s) 231, 258
ZrmI AGTACT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.