RLG00000009596

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000286
Physical Location & Seq
Reverse (-)
55121007 .. 55123615
2609 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009596

Sequence Viewer

Length: 1203 bp
ATGGATGTCAACGCGCGAGGCTTCCTGAATACTGCTTTAGCGTATTTCCAGAACTTGCCACAAAAAAACAAGTACGAGATAACTCAACTCTTTAGAGAGGCGGATACAAATGGTGATGGGACAATAAGCTTCGAGGAATTCCAGCAGCTCTTTGAAGATGCAACGTCGTCGTCATCTTCCCATGATGACGTTAAAAGCTTTTTGGGGAGATTTTTCAAGGAATTCGTTGACACAGACGGAGATGGTAGGTGGGATTATGACGAATTTATCGCTTATTTCTACCTTATTAAGAGTGGGAGGATGGAATTGTTGTGTAAAGGTTGCCATGGAGTATGTGATGGGAATGGATGTGAATCCCATTTCGTCAAGGGTGGACTGTACTTTGTTTGCGTTGATTGCTTTGACGATCCAAACATTGAAAACAGTTTCAATCTCTGTATCTCCTGCTACCGGGATGGAAAGTACACTCACCAGCATGAAAATTTTTTGGACAGCTTCGTCTTGCTATGTTCCAAATTTGTTCGTAAATCCACCGCACCGACCACAGTAACACAAAATCTTCAGATAGATCAAAAGATGCAAGAAATACGGTCTACTGCTTTCGCTTATTATGATAATATGCCATTGGAGTACAAGCAGAAGGCAAATGAAATTTTTAGAGAAATGGATTCAGATGGTGACGGGGCAATAACCCTCCAGCAATATGAGAAGAAATTTGGCAGCTCCACCAGGATGAAAAAGTGTTTCGATGAGGTGGACATAAACGGAGATGGCAGGTTGGATTTTTCAGAATTCATCACTATTTACTATCTTGACAAGGGTGGGAGGCTGAGAGTTTGGTGTAATGGCCATGGATGTATTCAAGCCCCTTTCCTCAAGGGAGCTTATTTTGTTTGCGTCAACTGCTTTGATAGTAATAGAGAAGAAAGTCCTTTCTACCTGTGCACTTTCTGCTATAGAAATGGAAAATACACTCACAGGCACACCAACTTTGTGGATAACTGCATGTTGCTGCAAGACAAGATGGCTCCTTACAGACCCGACAAAGGCCAAGCAGTAGCACAGAATCAATCAGCTGCACAAAATTATAAGGGGACGTTGGTCGTGGAAAAACAATCAGCCTCTCTACTAGGCATACTAGGCAACATTGCTAGTGTGGGGAGTTTTATTGTTGCTGCCAGTGGTGGGTGCAACATTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

401

Amino Acids

45.42

Weight (kDa)

5.29

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 15 - 51 3.2e-07 EF-hand domain pair
EF-hand_8 PF13833 23 - 53 1.6e-07 EF-hand domain pair
EF-hand_7 PF13499 25 - 94 1.8e-10 EF-hand domain pair
EF-hand_1 PF00036 26 - 52 3.2e-08 EF hand domain
EF-hand_6 PF13405 27 - 52 4.3e-06 EF-hand domain
EF-hand_5 PF13202 30 - 50 3.2e-08 EF hand
EF-hand_7 PF13499 213 - 270 8.6e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1089
AasI GACNNNNNNGTC 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 765
AccI GTMKAC 1 cut(s) 593
AccII CGCG 2 cut(s) 14, 16
AciI CCGC 2 cut(s) 101, 534
AclWI GGATC 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 847
AcsI RAATTY 8 cut(s) 137, 221, 263, 481, 515, 651, 713, 791
AcuI CTGAAG 1 cut(s) 545
AfaI GTAC 4 cut(s) 74, 380, 464, 632
AfiI CCNNNNNNNGG 3 cut(s) 450, 1046, 1185
AgsI TTSAA 5 cut(s) 155, 217, 419, 430, 863
AjnI CCWGG 1 cut(s) 728
AluBI AGCT 7 cut(s) 129, 148, 198, 495, 723, 884, 1076
AluI AGCT 7 cut(s) 129, 148, 198, 495, 723, 884, 1076
Alw21I GWGCWC 1 cut(s) 947
Alw44I GTGCAC 1 cut(s) 943
AlwI GGATC 1 cut(s) 401
AoxI GGCC 2 cut(s) 847, 1048
ApaLI GTGCAC 1 cut(s) 943
ApeKI GCWGC 5 cut(s) 145, 720, 1012, 1076, 1175
ApoI RAATTY 8 cut(s) 137, 221, 263, 481, 515, 651, 713, 791
AspLEI GCGC 1 cut(s) 16
AsuC2I CCSGG 1 cut(s) 452
AsuHPI GGTGA 3 cut(s) 125, 461, 689
BaeGI GKGCMC 1 cut(s) 947
BalI TGGCCA 1 cut(s) 849
Bbv12I GWGCWC 1 cut(s) 947
BbvI GCAGC 5 cut(s) 157, 732, 999, 1063, 1162
BccI CCATC 8 cut(s) 110, 236, 295, 332, 449, 668, 764, 1018
BcgI CGANNNNNNTGC 2 cut(s) 150, 184
BciT130I CCWGG 1 cut(s) 730
BciVI GTATCC 1 cut(s) 97
BcnI CCSGG 1 cut(s) 452
BfaI CTAG 3 cut(s) 1130, 1139, 1152
BfmI CTRYAG 1 cut(s) 955
BfuAI ACCTGC 1 cut(s) 765
BfuI GTATCC 1 cut(s) 97
BisI GCNGC 5 cut(s) 146, 721, 1013, 1077, 1176
BlsI GCNGC 5 cut(s) 147, 722, 1014, 1078, 1177
Bme1390I CCNGG 2 cut(s) 452, 730
BmiI GGNNCC 1 cut(s) 1029
BmrFI CCNGG 2 cut(s) 452, 730
BmsI GCATC 2 cut(s) 148, 567
BoxI GACNNNNGTC 1 cut(s) 1100
BpmI CTGGAG 1 cut(s) 680
BpuEI CTTGAG 1 cut(s) 860
BpuMI CCSGG 1 cut(s) 452
BsaBI GATNNNNATC 1 cut(s) 352
BsaJI CCNNGG 2 cut(s) 325, 850
Bsc4I CCNNNNNNNGG 3 cut(s) 450, 1046, 1185
Bse1I ACTGG 1 cut(s) 1179
Bse3DI GCAATG 1 cut(s) 1146
Bse8I GATNNNNATC 1 cut(s) 352
BseBI CCWGG 1 cut(s) 730
BseDI CCNNGG 2 cut(s) 325, 850
BseGI GGATG 6 cut(s) 10, 306, 353, 460, 738, 860
BseJI GATNNNNATC 1 cut(s) 352
BseLI CCNNNNNNNGG 3 cut(s) 450, 1046, 1185
BseMI GCAATG 1 cut(s) 1146
BseMII CTCAG 1 cut(s) 821
BseNI ACTGG 1 cut(s) 1179
BseSI GKGCMC 1 cut(s) 947
BseXI GCAGC 5 cut(s) 157, 732, 999, 1063, 1162
BsgI GTGCAG 1 cut(s) 1062
Bsh1236I CGCG 2 cut(s) 14, 16
BshFI GGCC 2 cut(s) 849, 1050
BsiHKAI GWGCWC 1 cut(s) 947
BsiSI CCGG 1 cut(s) 451
BslFI GGGAC 2 cut(s) 133, 1108
BslI CCNNNNNNNGG 3 cut(s) 450, 1046, 1185
BsmFI GGGAC 2 cut(s) 133, 1108
BsnI GGCC 2 cut(s) 849, 1050
Bsp1286I GDGCHC 1 cut(s) 947
Bsp143I GATC 2 cut(s) 406, 568
Bsp19I CCATGG 2 cut(s) 325, 850
BspACI CCGC 2 cut(s) 101, 534
BspANI GGCC 2 cut(s) 849, 1050
BspCNI CTCAG 1 cut(s) 822
BspFNI CGCG 2 cut(s) 14, 16
BspLI GGNNCC 1 cut(s) 1029
BspMI ACCTGC 1 cut(s) 765
BspPI GGATC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 1146
BsrI ACTGG 1 cut(s) 1179
BssECI CCNNGG 2 cut(s) 325, 850
BssMI GATC 2 cut(s) 406, 568
BssT1I CCWWGG 2 cut(s) 325, 850
Bst2UI CCWGG 1 cut(s) 730
Bst4CI ACNGT 4 cut(s) 378, 425, 547, 591
BstAPI GCANNNNNTGC 1 cut(s) 951
BstDEI CTNAG 1 cut(s) 830
BstDSI CCRYGG 2 cut(s) 325, 850
BstF5I GGATG 6 cut(s) 10, 306, 353, 460, 738, 860
BstFNI CGCG 2 cut(s) 14, 16
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 2 cut(s) 409, 571
BstMBI GATC 2 cut(s) 406, 568
BstMWI GCNNNNNNNGC 4 cut(s) 396, 903, 951, 1140
BstNI CCWGG 1 cut(s) 730
BstNSI RCATGY 1 cut(s) 1009
BstPAI GACNNNNGTC 1 cut(s) 1100
BstSCI CCNGG 2 cut(s) 450, 728
BstSFI CTRYAG 1 cut(s) 955
BstSLI GKGCMC 1 cut(s) 947
BstUI CGCG 2 cut(s) 14, 16
BstV1I GCAGC 5 cut(s) 157, 732, 999, 1063, 1162
BstXI CCANNNNNNTGG 1 cut(s) 994
BsuI GTATCC 1 cut(s) 97
BsuRI GGCC 2 cut(s) 849, 1050
BtgI CCRYGG 2 cut(s) 325, 850
BtsCI GGATG 6 cut(s) 10, 306, 353, 460, 738, 860
BtsIMutI CAGTG 1 cut(s) 1186
BveI ACCTGC 1 cut(s) 765
CfoI GCGC 1 cut(s) 16
CseI GACGC 1 cut(s) 886
Csp6I GTAC 4 cut(s) 73, 379, 463, 631
CviAII CATG 5 cut(s) 182, 326, 476, 851, 1006
CviQI GTAC 4 cut(s) 73, 379, 463, 631
DdeI CTNAG 1 cut(s) 830
DpnI GATC 2 cut(s) 408, 570
DpnII GATC 2 cut(s) 406, 568
DrdI GACNNNNNNGTC 1 cut(s) 497
DseDI GACNNNNNNGTC 1 cut(s) 497
EaeI YGGCCR 1 cut(s) 847
EciI GGCGGA 1 cut(s) 116
Eco130I CCWWGG 2 cut(s) 325, 850
Eco57I CTGAAG 1 cut(s) 545
EcoRI GAATTC 3 cut(s) 137, 221, 791
EcoRII CCWGG 1 cut(s) 728
EcoT14I CCWWGG 2 cut(s) 325, 850
ErhI CCWWGG 2 cut(s) 325, 850
FaeI CATG 5 cut(s) 185, 329, 479, 854, 1009
FaqI GGGAC 2 cut(s) 133, 1108
FatI CATG 5 cut(s) 181, 325, 475, 850, 1005
FblI GTMKAC 1 cut(s) 593
Fnu4HI GCNGC 5 cut(s) 146, 721, 1013, 1077, 1176
FokI GGATG 6 cut(s) 17, 313, 360, 467, 745, 867
Fsp4HI GCNGC 5 cut(s) 146, 721, 1013, 1077, 1176
FspBI CTAG 3 cut(s) 1130, 1139, 1152
GlaI GCGC 1 cut(s) 15
GluI GCNGC 5 cut(s) 146, 721, 1013, 1077, 1176
GsuI CTGGAG 1 cut(s) 680
HaeIII GGCC 2 cut(s) 849, 1050
HapII CCGG 1 cut(s) 451
HgaI GACGC 1 cut(s) 886
HhaI GCGC 1 cut(s) 16
Hin1II CATG 5 cut(s) 185, 329, 479, 854, 1009
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 3 cut(s) 10, 229, 901
HindII GTYRAC 3 cut(s) 10, 229, 901
HindIII AAGCTT 2 cut(s) 127, 196
HinfI GANTC 3 cut(s) 353, 668, 1066
HpaII CCGG 1 cut(s) 451
HphI GGTGA 3 cut(s) 125, 461, 689
Hpy166II GTNNAC 8 cut(s) 10, 229, 374, 465, 594, 757, 901, 945
Hpy188I TCNGA 3 cut(s) 564, 673, 790
Hpy188III TCNNGA 3 cut(s) 25, 49, 812
Hpy8I GTNNAC 8 cut(s) 10, 229, 374, 465, 594, 757, 901, 945
Hpy99I CGWCG 2 cut(s) 169, 172
HpyAV CCTTC 1 cut(s) 634
HpyCH4III ACNGT 4 cut(s) 378, 425, 547, 591
HpyCH4IV ACGT 3 cut(s) 164, 189, 1097
HpyCH4V TGCA 7 cut(s) 161, 580, 945, 1005, 1015, 1079, 1191
HpyF10VI GCNNNNNNNGC 4 cut(s) 396, 903, 951, 1140
HpyF3I CTNAG 1 cut(s) 830
HpySE526I ACGT 3 cut(s) 164, 189, 1097
Hsp92II CATG 5 cut(s) 185, 329, 479, 854, 1009
HspAI GCGC 1 cut(s) 14
Kzo9I GATC 2 cut(s) 406, 568
LmnI GCTCC 3 cut(s) 728, 881, 1033
Lsp1109I GCAGC 5 cut(s) 157, 732, 999, 1063, 1162
LweI GCATC 2 cut(s) 148, 567
MaeI CTAG 3 cut(s) 1130, 1139, 1152
MaeII ACGT 3 cut(s) 164, 189, 1097
MaeIII GTNAC 2 cut(s) 547, 677
MalI GATC 2 cut(s) 408, 570
MboI GATC 2 cut(s) 406, 568
MboII GAAGA 5 cut(s) 167, 168, 551, 721, 935
MhlI GDGCHC 1 cut(s) 947
MlsI TGGCCA 1 cut(s) 849
MluNI TGGCCA 1 cut(s) 849
MmeI TCCRAC 1 cut(s) 759
MnlI CCTC 9 cut(s) 11, 91, 127, 291, 704, 745, 819, 884, 1132
Mox20I TGGCCA 1 cut(s) 849
MscI TGGCCA 1 cut(s) 849
MseI TTAA 2 cut(s) 192, 288
MslI CAYNNNNRTG 2 cut(s) 474, 731
Msp20I TGGCCA 1 cut(s) 849
MspA1I CMGCKG 1 cut(s) 1076
MspI CCGG 1 cut(s) 451
MspR9I CCNGG 2 cut(s) 452, 730
MvaI CCWGG 1 cut(s) 730
MvnI CGCG 2 cut(s) 14, 16
MwoI GCNNNNNNNGC 4 cut(s) 396, 903, 951, 1140
NciI CCSGG 1 cut(s) 452
NcoI CCATGG 2 cut(s) 325, 850
NdeII GATC 2 cut(s) 406, 568
NlaIII CATG 5 cut(s) 185, 329, 479, 854, 1009
NlaIV GGNNCC 1 cut(s) 1029
NmuCI GTSAC 1 cut(s) 677
NspI RCATGY 1 cut(s) 1009
PfeI GAWTC 3 cut(s) 353, 668, 1066
PkrI GCNGC 5 cut(s) 147, 722, 1014, 1078, 1177
PshAI GACNNNNGTC 1 cut(s) 1100
PsiI TTATAA 1 cut(s) 1089
Psp6I CCWGG 1 cut(s) 728
PspGI CCWGG 1 cut(s) 728
PspN4I GGNNCC 1 cut(s) 1029
PvuII CAGCTG 1 cut(s) 1076
RsaI GTAC 4 cut(s) 74, 380, 464, 632
RsaNI GTAC 4 cut(s) 73, 379, 463, 631
RseI CAYNNNNRTG 2 cut(s) 474, 731
SaqAI TTAA 2 cut(s) 192, 288
SatI GCNGC 5 cut(s) 146, 721, 1013, 1077, 1176
Sau3AI GATC 2 cut(s) 406, 568
ScrFI CCNGG 2 cut(s) 452, 730
SduI GDGCHC 1 cut(s) 947
SfaNI GCATC 2 cut(s) 148, 567
SfcI CTRYAG 1 cut(s) 955
SmiMI CAYNNNNRTG 2 cut(s) 474, 731
SmlI CTYRAG 1 cut(s) 875
SmoI CTYRAG 1 cut(s) 875
SsiI CCGC 2 cut(s) 101, 534
SspMI CTAG 3 cut(s) 1130, 1139, 1152
StyD4I CCNGG 2 cut(s) 450, 728
StyI CCWWGG 2 cut(s) 325, 850
TaaI ACNGT 4 cut(s) 378, 425, 547, 591
TaiI ACGT 3 cut(s) 167, 192, 1100
TaqI TCGA 2 cut(s) 132, 747
TatI WGTACW 3 cut(s) 378, 462, 630
TfiI GAWTC 3 cut(s) 353, 668, 1066
Tru1I TTAA 2 cut(s) 192, 288
Tru9I TTAA 2 cut(s) 192, 288
TscAI CASTG 1 cut(s) 1186
TseFI GTSAC 1 cut(s) 677
TseI GCWGC 5 cut(s) 145, 720, 1012, 1076, 1175
Tsp45I GTSAC 1 cut(s) 677
TspDTI ATGAA 4 cut(s) 492, 663, 749, 784
TspGWI ACGGA 2 cut(s) 252, 780
TspRI CASTG 1 cut(s) 1186
VneI GTGCAC 1 cut(s) 943
XapI RAATTY 8 cut(s) 137, 221, 263, 481, 515, 651, 713, 791
XceI RCATGY 1 cut(s) 1009
XmiI GTMKAC 1 cut(s) 593
XspI CTAG 3 cut(s) 1130, 1139, 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.