Rroxscaffold_5G00340630

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000286
Physical Location & Seq
Forward (+)
9103166 .. 9104603
1438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00340630.1

Sequence Viewer

Length: 642 bp
ATGGCAATGGAAGAACTACATGCAGCTGCTTTAGCATACTACGACAATGGCACTCAACAGCTTCGGGATCTGGCAGGGTCTTTCTTCCGATCTATGGACACCAACGGGGATGGTCAAATCAGCTGCTTGGAGTTCAATGACTTCCTCCAGCAAAGTGGCTACAACTGGATTATTCACGATCCAAACTTCTTCAAAAGACTTGACCGCAATGGCGATGGTGGATTGGATTTTGGGGAAGTTCTCACTTTCTATTATATCATTAAAACGAGGTACATCAAGTGCCAAGGGTATCGGTGCGGTGTACACCTCTGTGGTTTGTATTTCACTTGCGTTGCTTGCTTTGATGGAGCTCATGAGCATCGCTCCACCTTTGATCTCTGTCCTGCCTGCTATCGCAATCGGAATTACTATCACCACCAGCACCACACATACTTCTTGGATAATCATGTATTGCTGCGCTCCAAACGAGGCATCTCTCCATATGCTCCACCAGATCTCAACATGGCAATGACTCCAACCCCGCCTCCAGCAGTTTATAATAATATTAATTACTACATCGCTGCTGCACCTCAAAGGGTATTTCGCGTGTTTGAGATGGCTCTTGCTGCTGCTAGTGTGGCTGCTAATTGTACAATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

213

Amino Acids

24.35

Weight (kDa)

6.53

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 537
AccII CGCG 1 cut(s) 585
AciI CCGC 3 cut(s) 205, 297, 521
AclWI GGATC 2 cut(s) 75, 173
AfaI GTAC 3 cut(s) 272, 303, 631
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 2 cut(s) 136, 193
AleI CACNNNNGTG 1 cut(s) 309
AluBI AGCT 4 cut(s) 26, 61, 123, 350
AluI AGCT 4 cut(s) 26, 61, 123, 350
Alw21I GWGCWC 1 cut(s) 352
AlwI GGATC 2 cut(s) 75, 173
ApeKI GCWGC 9 cut(s) 23, 26, 123, 454, 560, 563, 605, 608, 620
AseI ATTAAT 1 cut(s) 546
AspLEI GCGC 1 cut(s) 459
AsuHPI GGTGA 1 cut(s) 404
BanII GRGCYC 1 cut(s) 352
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 9 cut(s) 13, 35, 110, 441, 547, 550, 592, 595, 607
BccI CCATC 4 cut(s) 104, 209, 338, 589
BfaI CTAG 1 cut(s) 612
BglII AGATCT 1 cut(s) 493
BisI GCNGC 9 cut(s) 24, 27, 124, 455, 561, 564, 606, 609, 621
BlsI GCNGC 9 cut(s) 25, 28, 125, 456, 562, 565, 607, 610, 622
BmsI GCATC 2 cut(s) 367, 480
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 2 cut(s) 131, 510
BsaJI CCNNGG 1 cut(s) 283
BsaXI ACNNNNNCTCC 2 cut(s) 508, 538
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 3 cut(s) 12, 214, 513
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 3 cut(s) 12, 214, 513
BseNI ACTGG 1 cut(s) 170
BseXI GCAGC 9 cut(s) 13, 35, 110, 441, 547, 550, 592, 595, 607
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 352
BslI CCNNNNNNNGG 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 352
Bsp1407I TGTACA 2 cut(s) 301, 629
Bsp143I GATC 5 cut(s) 67, 89, 178, 373, 493
BspACI CCGC 3 cut(s) 205, 297, 521
BspFNI CGCG 1 cut(s) 585
BspHI TCATGA 1 cut(s) 352
BspPI GGATC 2 cut(s) 75, 173
BsrDI GCAATG 3 cut(s) 12, 214, 513
BsrGI TGTACA 2 cut(s) 301, 629
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 5 cut(s) 67, 89, 178, 373, 493
BssT1I CCWWGG 1 cut(s) 283
BstAUI TGTACA 2 cut(s) 301, 629
BstC8I GCNNGC 2 cut(s) 337, 388
BstF5I GGATG 1 cut(s) 115
BstFNI CGCG 1 cut(s) 585
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 5 cut(s) 70, 92, 181, 376, 496
BstMBI GATC 5 cut(s) 67, 89, 178, 373, 493
BstMWI GCNNNNNNNGC 4 cut(s) 32, 336, 605, 617
BstNSI RCATGY 1 cut(s) 23
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 9 cut(s) 13, 35, 110, 441, 547, 550, 592, 595, 607
BstX2I RGATCY 2 cut(s) 67, 493
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 2 cut(s) 67, 493
BtgZI GCGATG 3 cut(s) 228, 344, 541
BtsCI GGATG 1 cut(s) 115
Cac8I GCNNGC 2 cut(s) 337, 388
CciI TCATGA 1 cut(s) 352
CfoI GCGC 1 cut(s) 459
Csp6I GTAC 3 cut(s) 271, 302, 630
CviAII CATG 5 cut(s) 20, 353, 446, 502, 637
CviJI RGCY 7 cut(s) 26, 61, 123, 159, 350, 599, 620
CviKI_1 RGCY 7 cut(s) 26, 61, 123, 159, 350, 599, 620
CviQI GTAC 3 cut(s) 271, 302, 630
DpnI GATC 5 cut(s) 69, 91, 180, 375, 495
DpnII GATC 5 cut(s) 67, 89, 178, 373, 493
Ecl136II GAGCTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 350
EcoICRI GAGCTC 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 5 cut(s) 23, 356, 449, 505, 640
FatI CATG 5 cut(s) 19, 352, 445, 501, 636
FauI CCCGC 1 cut(s) 528
FauNDI CATATG 1 cut(s) 481
Fnu4HI GCNGC 9 cut(s) 24, 27, 124, 455, 561, 564, 606, 609, 621
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 9 cut(s) 24, 27, 124, 455, 561, 564, 606, 609, 621
FspBI CTAG 1 cut(s) 612
GlaI GCGC 1 cut(s) 458
GluI GCNGC 9 cut(s) 24, 27, 124, 455, 561, 564, 606, 609, 621
GsuI CTGGAG 2 cut(s) 131, 510
HhaI GCGC 1 cut(s) 459
Hin1II CATG 5 cut(s) 23, 356, 449, 505, 640
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HinfI GANTC 1 cut(s) 511
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 2 cut(s) 302, 304
Hpy188I TCNGA 2 cut(s) 89, 402
Hpy188III TCNNGA 3 cut(s) 65, 176, 353
Hpy8I GTNNAC 2 cut(s) 302, 304
HpyCH4V TGCA 2 cut(s) 23, 566
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 336, 605, 617
Hsp92II CATG 5 cut(s) 23, 356, 449, 505, 640
HspAI GCGC 1 cut(s) 457
Kzo9I GATC 5 cut(s) 67, 89, 178, 373, 493
LmnI GCTCC 4 cut(s) 347, 368, 464, 490
LpnPI CCDG 9 cut(s) 56, 60, 151, 161, 396, 400, 431, 504, 540
Lsp1109I GCAGC 9 cut(s) 13, 35, 110, 441, 547, 550, 592, 595, 607
LweI GCATC 2 cut(s) 367, 480
MaeI CTAG 1 cut(s) 612
MalI GATC 5 cut(s) 69, 91, 180, 375, 495
MboI GATC 5 cut(s) 67, 89, 178, 373, 493
MboII GAAGA 3 cut(s) 23, 76, 181
MflI RGATCY 2 cut(s) 67, 493
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 3 cut(s) 403, 547, 625
MlyI GAGTC 1 cut(s) 505
MmeI TCCRAC 1 cut(s) 539
MnlI CCTC 6 cut(s) 155, 261, 317, 461, 534, 579
MseI TTAA 2 cut(s) 261, 546
MslI CAYNNNNRTG 2 cut(s) 309, 506
MspA1I CMGCKG 2 cut(s) 26, 123
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 4 cut(s) 32, 336, 605, 617
NdeI CATATG 1 cut(s) 481
NdeII GATC 5 cut(s) 67, 89, 178, 373, 493
NlaIII CATG 5 cut(s) 23, 356, 449, 505, 640
NspI RCATGY 1 cut(s) 23
OliI CACNNNNGTG 1 cut(s) 309
PagI TCATGA 1 cut(s) 352
PkrI GCNGC 9 cut(s) 25, 28, 125, 456, 562, 565, 607, 610, 622
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
PshBI ATTAAT 1 cut(s) 546
PsiI TTATAA 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 352
PsuI RGATCY 2 cut(s) 67, 493
PvuII CAGCTG 2 cut(s) 26, 123
RsaI GTAC 3 cut(s) 272, 303, 631
RsaNI GTAC 3 cut(s) 271, 302, 630
RseI CAYNNNNRTG 2 cut(s) 309, 506
SacI GAGCTC 1 cut(s) 352
SaqAI TTAA 2 cut(s) 261, 546
SatI GCNGC 9 cut(s) 24, 27, 124, 455, 561, 564, 606, 609, 621
Sau3AI GATC 5 cut(s) 67, 89, 178, 373, 493
SchI GAGTC 1 cut(s) 505
SduI GDGCHC 1 cut(s) 352
SetI ASST 8 cut(s) 28, 63, 125, 272, 309, 352, 371, 571
SfaNI GCATC 2 cut(s) 367, 480
SmiMI CAYNNNNRTG 2 cut(s) 309, 506
Sse9I AATT 3 cut(s) 403, 547, 625
SsiI CCGC 3 cut(s) 205, 297, 521
SspI AATATT 1 cut(s) 544
SspMI CTAG 1 cut(s) 612
SstI GAGCTC 1 cut(s) 352
StyI CCWWGG 1 cut(s) 283
TasI AATT 3 cut(s) 403, 547, 625
TatI WGTACW 2 cut(s) 301, 629
Tru1I TTAA 2 cut(s) 261, 546
Tru9I TTAA 2 cut(s) 261, 546
TseI GCWGC 9 cut(s) 23, 26, 123, 454, 560, 563, 605, 608, 620
VspI ATTAAT 1 cut(s) 546
XceI RCATGY 1 cut(s) 23
XspI CTAG 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.