Rmu_sc0011701.1_g000009

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011701.1
OG0000286
Physical Location & Seq
Reverse (-)
24064 .. 24561
498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011701.1_g000009.1.cds

Sequence Viewer

Length: 498 bp
atgggagacatcaaagaaatccgtgatgctgctttagcttattaccagaatttatctacaaagcaacagcaagaggcgtacagtttttttcaaacaatggataaagatgatgatggaacagtaaccatggaggagttcaagcagatgtttgagggttattttggcaacaatgtcagccatgccttccaggacctagacaaaaatggagatggcaatttggattttgaggaatctatcactctttactactctcttaaggctcggaggatcactttttgcaatggatgcaaagccttttttctaaagggactgtatttcacttgcgtggactgcttcaagcataaccaaactacttttgatctttgcacctcctgctaccgcatccgcaactacgttcacgaacactgcaactttttggacaactacgccttgctagctacttatagattgacaaaccaggtgatttatcaattgaactcttacagattagaagtctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

165

Amino Acids

19.44

Weight (kDa)

5.14

Isoelectric Point (pI)

20.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 379, 385
AclWI GGATC 1 cut(s) 275
AcsI RAATTY 1 cut(s) 49
AfaI GTAC 1 cut(s) 80
AflII CTTAAG 1 cut(s) 254
AgsI TTSAA 4 cut(s) 92, 139, 337, 475
AjnI CCWGG 2 cut(s) 186, 456
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 2 cut(s) 38, 437
AluI AGCT 2 cut(s) 38, 437
AlwI GGATC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 49
AspS9I GGNCC 1 cut(s) 190
AsuHPI GGTGA 1 cut(s) 472
AsuNHI GCTAGC 1 cut(s) 433
AvaII GGWCC 1 cut(s) 190
BbvI GCAGC 1 cut(s) 16
BccI CCATC 2 cut(s) 107, 203
BciT130I CCWGG 2 cut(s) 188, 458
BfaI CTAG 2 cut(s) 194, 434
BfrI CTTAAG 1 cut(s) 254
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 2 cut(s) 188, 458
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 190
BmrFI CCNGG 2 cut(s) 188, 458
BmsI GCATC 3 cut(s) 16, 275, 390
BmtI GCTAGC 1 cut(s) 437
BsaJI CCNNGG 1 cut(s) 126
Bse3DI GCAATG 1 cut(s) 286
BseBI CCWGG 2 cut(s) 188, 458
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 2 cut(s) 290, 381
BseMI GCAATG 1 cut(s) 286
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 16
BslFI GGGAC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 321
Bsp143I GATC 2 cut(s) 267, 358
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 2 cut(s) 379, 385
BspOI GCTAGC 1 cut(s) 437
BspPI GGATC 1 cut(s) 275
BspTI CTTAAG 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 286
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 2 cut(s) 267, 358
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 2 cut(s) 188, 458
Bst4CI ACNGT 3 cut(s) 83, 121, 312
BstAFI CTTAAG 1 cut(s) 254
BstAPI GCANNNNNTGC 2 cut(s) 285, 372
BstC8I GCNNGC 1 cut(s) 435
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 2 cut(s) 290, 381
BstKTI GATC 2 cut(s) 270, 361
BstMBI GATC 2 cut(s) 267, 358
BstMWI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 372, 434
BstNI CCWGG 2 cut(s) 188, 458
BstSCI CCNGG 2 cut(s) 186, 456
BstV1I GCAGC 1 cut(s) 16
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 2 cut(s) 290, 381
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 403
Cac8I GCNNGC 1 cut(s) 435
Cfr13I GGNCC 1 cut(s) 190
CsiI ACCWGGT 1 cut(s) 456
Csp6I GTAC 1 cut(s) 79
CviAII CATG 2 cut(s) 127, 179
CviJI RGCY 5 cut(s) 38, 177, 260, 293, 437
CviKI_1 RGCY 5 cut(s) 38, 177, 260, 293, 437
CviQI GTAC 1 cut(s) 79
DpnI GATC 2 cut(s) 269, 360
DpnII GATC 2 cut(s) 267, 358
Eco130I CCWWGG 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 190
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 2 cut(s) 186, 456
EcoT14I CCWWGG 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 2 cut(s) 130, 182
FaiI YATR 4 cut(s) 128, 180, 342, 444
FaqI GGGAC 1 cut(s) 321
FatI CATG 2 cut(s) 126, 178
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 2 cut(s) 297, 368
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 2 cut(s) 194, 434
GluI GCNGC 1 cut(s) 30
Hin1II CATG 2 cut(s) 130, 182
HinfI GANTC 1 cut(s) 230
HphI GGTGA 1 cut(s) 472
Hpy166II GTNNAC 2 cut(s) 328, 397
Hpy188I TCNGA 1 cut(s) 264
Hpy188III TCNNGA 1 cut(s) 398
Hpy8I GTNNAC 2 cut(s) 328, 397
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 3 cut(s) 83, 121, 312
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 4 cut(s) 279, 288, 366, 408
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 372, 434
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 2 cut(s) 130, 182
Kzo9I GATC 2 cut(s) 267, 358
LpnPI CCDG 6 cut(s) 59, 173, 200, 385, 443, 470
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 16, 275, 390
MabI ACCWGGT 1 cut(s) 456
MaeI CTAG 2 cut(s) 194, 434
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 1 cut(s) 121
MalI GATC 2 cut(s) 269, 360
MboI GATC 2 cut(s) 267, 358
MfeI CAATTG 1 cut(s) 470
MluCI AATT 3 cut(s) 49, 214, 470
MnlI CCTC 6 cut(s) 67, 124, 145, 220, 258, 379
MseI TTAA 1 cut(s) 255
MslI CAYNNNNRTG 1 cut(s) 323
MspCI CTTAAG 1 cut(s) 254
MspR9I CCNGG 2 cut(s) 188, 458
MunI CAATTG 1 cut(s) 470
MvaI CCWGG 2 cut(s) 188, 458
MwoI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 372, 434
NcoI CCATGG 1 cut(s) 126
NdeII GATC 2 cut(s) 267, 358
NheI GCTAGC 1 cut(s) 433
NlaIII CATG 2 cut(s) 130, 182
OliI CACNNNNGTG 1 cut(s) 323
PfeI GAWTC 1 cut(s) 230
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 190
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 186, 456
PspGI CCWGG 2 cut(s) 186, 456
PspPI GGNCC 1 cut(s) 190
PspPPI RGGWCCY 1 cut(s) 190
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 2 cut(s) 267, 358
Sau96I GGNCC 1 cut(s) 190
ScrFI CCNGG 2 cut(s) 188, 458
SetI ASST 6 cut(s) 40, 195, 371, 396, 439, 462
SexAI ACCWGGT 1 cut(s) 456
SfaNI GCATC 3 cut(s) 16, 275, 390
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 3 cut(s) 49, 214, 470
SsiI CCGC 2 cut(s) 379, 385
SspMI CTAG 2 cut(s) 194, 434
StyD4I CCNGG 2 cut(s) 186, 456
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 3 cut(s) 83, 121, 312
TaiI ACGT 1 cut(s) 396
TasI AATT 3 cut(s) 49, 214, 470
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 1 cut(s) 410
TseI GCWGC 1 cut(s) 29
TspGWI ACGGA 1 cut(s) 11
TspRI CASTG 1 cut(s) 410
Vha464I CTTAAG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 190
XapI RAATTY 1 cut(s) 49
XspI CTAG 2 cut(s) 194, 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.