Rh4DG056600

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000286
Physical Location & Seq
Forward (+)
8746762 .. 8748592
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG056600.1

Sequence Viewer

Length: 1161 bp
ATGGAAGAAATACGTGAAGTTGCTTTAGCATACTATGACAATTGCACACCAGAACTTCGGGGTAAGACATGGGAGTTCTTTCAATCCATGGATACGAACGGCGATCACCGAATCAGTCCCAGGGAGTTCAATGAATTCCTCCGTCAGACCGGTTACAACACGATCATTACTGACCCAAAATTATTTGCAATGCTTGACCGCAATGGTGACAGCGGGCTGGATTTTTCTGAATTTCTCACTTTCTACTATATCATTAAAACTGGGTACATCTGTTGCCGAGGTTGCCGCATGTACCTTTCCGGGCTTTATTTTACGTGCTTTGAATGCTTTGAGGCAGCCAATCCCTCCACTTTTGATCTATGTGATGCTTGTCATCGCAGTGGGAACGTTACTCACCACCACAACGTTACTCACCACCACACACTCTTCTTGGAGAACCACATATTGCTATACTACAAAAGAGGACCAGATGACAGCATGGCAATGGAATATCTATATGCACAAGCTTTAGAGTACTATGATAAAGCAACTCCAGAGCTTAAGAGTTCAGTGATGTCTTTCTTTCAATCCATGGTTACACACGGTGCAAACCAAGTCTCTGTGTTCAACGATTTCCTCAAGCAAAAAGGCTACAACTGGATTATTAATGATCCACTCTTGTTCCAGAAACTCGACCGCAACCGTGATGGCGGCCTCGATTTCTGGGAATTGCTCACCTTCTACTACATCATCAACACAAGGTACTTCAATTGCCGAGAATGTCAGGTGTACCTTGGCGGGCTGTATTTTACCTGCGTTGCTTGCTTTGAGGGAGGAGCTCATTATCGTCGTCATCATCATTCTGATCTCGGCACCTATAATCTATGTGCCGCCTGCTATCGCAGTAGGAATTTCGTCTCTCAACACCCGCACCACACCTTCTTCTTGGATAACCACATATTGCTGCGCTCCAAAAGGGGTCTTCCTCCTTATGCTACACCAAGTCTGGTAAGCGCTCCAAAACCTCTGGTGGTTTATAATAATTATTATTTTATTAATAATAATTACCACCTTCCAGCTGTGCAGCCTGTACCTCCAAAGCAGAGTTGGCTATCGTCACTCAACCTATTAGAGGTGGCCCTGGCTGGTGCAAACTTGGCTGCTAATTGTTCGATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

386

Amino Acids

44.83

Weight (kDa)

6.43

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 26 - 83 2.3e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
Acc36I ACCTGC 1 cut(s) 800
AccB1I GGYRCC 1 cut(s) 851
AciI CCGC 8 cut(s) 199, 213, 286, 676, 690, 777, 870, 908
AclI AACGTT 2 cut(s) 387, 405
AclWI GGATC 1 cut(s) 644
AcsI RAATTY 3 cut(s) 134, 230, 889
AdeI CACNNNGTG 1 cut(s) 584
AfaI GTAC 6 cut(s) 266, 293, 515, 743, 770, 1071
AfeI AGCGCT 1 cut(s) 994
AfiI CCNNNNNNNGG 1 cut(s) 1111
AflII CTTAAG 1 cut(s) 539
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 6 cut(s) 83, 130, 323, 566, 607, 748
AjnI CCWGG 2 cut(s) 119, 1119
AluBI AGCT 4 cut(s) 506, 538, 818, 1058
AluI AGCT 4 cut(s) 506, 538, 818, 1058
Alw21I GWGCWC 1 cut(s) 820
Alw26I GTCTC 2 cut(s) 601, 901
AlwI GGATC 1 cut(s) 644
Aor51HI AGCGCT 1 cut(s) 994
AoxI GGCC 2 cut(s) 691, 1116
ApeKI GCWGC 4 cut(s) 335, 943, 1063, 1139
ApoI RAATTY 3 cut(s) 134, 230, 889
AseI ATTAAT 2 cut(s) 645, 1035
AsiGI ACCGGT 1 cut(s) 149
AspLEI GCGC 2 cut(s) 948, 995
AspS9I GGNCC 2 cut(s) 464, 1117
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 5 cut(s) 98, 218, 386, 404, 706
AvaII GGWCC 1 cut(s) 464
BaeI ACNNNNGTAYC 2 cut(s) 256, 289
BanI GGYRCC 1 cut(s) 851
BanII GRGCYC 1 cut(s) 820
BbsI GAAGAC 1 cut(s) 953
Bbv12I GWGCWC 1 cut(s) 820
BbvI GCAGC 4 cut(s) 347, 930, 1075, 1126
BccI CCATC 1 cut(s) 680
BceAI ACGGC 1 cut(s) 115
BciT130I CCWGG 2 cut(s) 121, 1121
BciVI GTATCC 1 cut(s) 85
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 2 cut(s) 601, 901
BfoI RGCGCY 1 cut(s) 996
BfrI CTTAAG 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 800
BfuI GTATCC 1 cut(s) 85
BisI GCNGC 7 cut(s) 286, 336, 691, 870, 944, 1064, 1140
BlsI GCNGC 7 cut(s) 287, 337, 692, 871, 945, 1065, 1141
BmcAI AGTACT 1 cut(s) 515
Bme1390I CCNGG 3 cut(s) 121, 301, 1121
Bme18I GGWCC 1 cut(s) 464
BmgT120I GGNCC 2 cut(s) 464, 1117
BmiI GGNNCC 1 cut(s) 853
BmrFI CCNGG 3 cut(s) 121, 301, 1121
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 355
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 953
BpmI CTGGAG 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 1 cut(s) 301
BsaAI YACGTR 2 cut(s) 14, 315
BsaJI CCNNGG 7 cut(s) 87, 119, 120, 277, 570, 772, 1119
BsaWI WCCGGW 1 cut(s) 149
Bsc4I CCNNNNNNNGG 1 cut(s) 1111
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 2 cut(s) 265, 641
Bse3DI GCAATG 3 cut(s) 195, 208, 489
BseBI CCWGG 2 cut(s) 121, 1121
BseDI CCNNGG 7 cut(s) 87, 119, 120, 277, 570, 772, 1119
BseLI CCNNNNNNNGG 1 cut(s) 1111
BseMI GCAATG 3 cut(s) 195, 208, 489
BseNI ACTGG 2 cut(s) 265, 641
BseRI GAGGAG 1 cut(s) 828
BseXI GCAGC 4 cut(s) 347, 930, 1075, 1126
BsgI GTGCAG 1 cut(s) 1082
Bsh1285I CGRYCG 1 cut(s) 676
BshFI GGCC 2 cut(s) 693, 1118
BshNI GGYRCC 1 cut(s) 851
BshTI ACCGGT 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 676
BsiHKAI GWGCWC 1 cut(s) 820
BsiSI CCGG 2 cut(s) 150, 300
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 1111
BsmAI GTCTC 2 cut(s) 601, 901
BsmBI CGTCTC 1 cut(s) 901
BsmFI GGGAC 1 cut(s) 102
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 2 cut(s) 693, 1118
Bsp1286I GDGCHC 1 cut(s) 820
Bsp143I GATC 5 cut(s) 103, 162, 355, 649, 844
Bsp19I CCATGG 2 cut(s) 87, 570
BspACI CCGC 8 cut(s) 199, 213, 286, 676, 690, 777, 870, 908
BspANI GGCC 2 cut(s) 693, 1118
BspLI GGNNCC 1 cut(s) 853
BspMI ACCTGC 1 cut(s) 800
BspPI GGATC 1 cut(s) 644
BspT107I GGYRCC 1 cut(s) 851
BspTI CTTAAG 1 cut(s) 539
BsrDI GCAATG 3 cut(s) 195, 208, 489
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 2 cut(s) 265, 641
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 7 cut(s) 87, 119, 120, 277, 570, 772, 1119
BssMI GATC 5 cut(s) 103, 162, 355, 649, 844
BssT1I CCWWGG 3 cut(s) 87, 570, 772
Bst2UI CCWGG 2 cut(s) 121, 1121
Bst4CI ACNGT 2 cut(s) 584, 683
Bst6I CTCTTC 1 cut(s) 431
BstAFI CTTAAG 1 cut(s) 539
BstBAI YACGTR 2 cut(s) 14, 315
BstC8I GCNNGC 4 cut(s) 215, 779, 802, 874
BstDSI CCRYGG 2 cut(s) 87, 570
BstENI CCTNNNNNAGG 1 cut(s) 1109
BstH2I RGCGCY 1 cut(s) 996
BstHHI GCGC 2 cut(s) 948, 995
BstKTI GATC 5 cut(s) 106, 165, 358, 652, 847
BstMAI GTCTC 2 cut(s) 601, 901
BstMBI GATC 5 cut(s) 103, 162, 355, 649, 844
BstMCI CGRYCG 1 cut(s) 676
BstMWI GCNNNNNNNGC 5 cut(s) 282, 324, 801, 1087, 1136
BstNI CCWGG 2 cut(s) 121, 1121
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 3 cut(s) 119, 299, 1119
BstV1I GCAGC 4 cut(s) 347, 930, 1075, 1126
BstV2I GAAGAC 1 cut(s) 953
BsuI GTATCC 1 cut(s) 85
BsuRI GGCC 2 cut(s) 693, 1118
BtgI CCRYGG 2 cut(s) 87, 570
BtgZI GCGATG 1 cut(s) 359
BtsI GCAGTG 1 cut(s) 385
BtsIMutI CAGTG 2 cut(s) 385, 555
BveI ACCTGC 1 cut(s) 800
Cac8I GCNNGC 4 cut(s) 215, 779, 802, 874
CfoI GCGC 2 cut(s) 948, 995
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 464, 1117
Csp6I GTAC 6 cut(s) 265, 292, 514, 742, 769, 1070
CspAI ACCGGT 1 cut(s) 149
CviAII CATG 5 cut(s) 69, 88, 289, 478, 571
CviQI GTAC 6 cut(s) 265, 292, 514, 742, 769, 1070
DpnI GATC 5 cut(s) 105, 164, 357, 651, 846
DpnII GATC 5 cut(s) 103, 162, 355, 649, 844
DraIII CACNNNGTG 1 cut(s) 584
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
Ecl136II GAGCTC 1 cut(s) 818
Eco130I CCWWGG 3 cut(s) 87, 570, 772
Eco24I GRGCYC 1 cut(s) 820
Eco47I GGWCC 1 cut(s) 464
Eco47III AGCGCT 1 cut(s) 994
Eco53kI GAGCTC 1 cut(s) 818
EcoICRI GAGCTC 1 cut(s) 818
EcoNI CCTNNNNNAGG 1 cut(s) 1109
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 119, 1119
EcoT14I CCWWGG 3 cut(s) 87, 570, 772
EcoT38I GRGCYC 1 cut(s) 820
ErhI CCWWGG 3 cut(s) 87, 570, 772
Esp3I CGTCTC 1 cut(s) 901
FaeI CATG 5 cut(s) 72, 91, 292, 481, 574
FaqI GGGAC 1 cut(s) 102
FatI CATG 5 cut(s) 68, 87, 288, 477, 570
FauI CCCGC 3 cut(s) 206, 770, 915
Fnu4HI GCNGC 7 cut(s) 286, 336, 691, 870, 944, 1064, 1140
FriOI GRGCYC 1 cut(s) 820
Fsp4HI GCNGC 7 cut(s) 286, 336, 691, 870, 944, 1064, 1140
GlaI GCGC 2 cut(s) 947, 994
GluI GCNGC 7 cut(s) 286, 336, 691, 870, 944, 1064, 1140
GsuI CTGGAG 1 cut(s) 516
HaeII RGCGCY 1 cut(s) 996
HaeIII GGCC 2 cut(s) 693, 1118
HapII CCGG 2 cut(s) 150, 300
HhaI GCGC 2 cut(s) 948, 995
Hin1II CATG 5 cut(s) 72, 91, 292, 481, 574
Hin6I GCGC 2 cut(s) 946, 993
HinP1I GCGC 2 cut(s) 946, 993
HindIII AAGCTT 1 cut(s) 504
HinfI GANTC 1 cut(s) 111
HpaII CCGG 2 cut(s) 150, 300
HphI GGTGA 5 cut(s) 98, 218, 386, 404, 706
Hpy166II GTNNAC 1 cut(s) 769
Hpy188I TCNGA 3 cut(s) 147, 229, 844
Hpy188III TCNNGA 2 cut(s) 533, 664
Hpy8I GTNNAC 1 cut(s) 769
Hpy99I CGWCG 1 cut(s) 831
HpyAV CCTTC 3 cut(s) 727, 928, 1061
HpyCH4III ACNGT 2 cut(s) 584, 683
HpyCH4IV ACGT 4 cut(s) 13, 314, 387, 405
HpyCH4V TGCA 6 cut(s) 45, 188, 500, 587, 1063, 1130
HpyF10VI GCNNNNNNNGC 5 cut(s) 282, 324, 801, 1087, 1136
HpySE526I ACGT 4 cut(s) 13, 314, 387, 405
Hsp92II CATG 5 cut(s) 72, 91, 292, 481, 574
HspAI GCGC 2 cut(s) 946, 993
Kzo9I GATC 5 cut(s) 103, 162, 355, 649, 844
LmnI GCTCC 3 cut(s) 815, 953, 1000
Lsp1109I GCAGC 4 cut(s) 347, 930, 1075, 1126
LweI GCATC 1 cut(s) 355
MaeII ACGT 4 cut(s) 13, 314, 387, 405
MaeIII GTNAC 6 cut(s) 152, 206, 388, 406, 574, 1095
MalI GATC 5 cut(s) 105, 164, 357, 651, 846
MboI GATC 5 cut(s) 103, 162, 355, 649, 844
MboII GAAGA 4 cut(s) 17, 418, 913, 953
MfeI CAATTG 2 cut(s) 40, 748
MhlI GDGCHC 1 cut(s) 820
MseI TTAA 4 cut(s) 255, 540, 645, 1035
MslI CAYNNNNRTG 2 cut(s) 378, 482
MspA1I CMGCKG 2 cut(s) 213, 1058
MspCI CTTAAG 1 cut(s) 539
MspI CCGG 2 cut(s) 150, 300
MspR9I CCNGG 3 cut(s) 121, 301, 1121
MunI CAATTG 2 cut(s) 40, 748
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 2 cut(s) 121, 1121
MwoI GCNNNNNNNGC 5 cut(s) 282, 324, 801, 1087, 1136
NciI CCSGG 1 cut(s) 301
NcoI CCATGG 2 cut(s) 87, 570
NdeII GATC 5 cut(s) 103, 162, 355, 649, 844
NlaIII CATG 5 cut(s) 72, 91, 292, 481, 574
NlaIV GGNNCC 1 cut(s) 853
NmeAIII GCCGAG 3 cut(s) 302, 779, 828
NmuCI GTSAC 2 cut(s) 206, 1095
NspI RCATGY 1 cut(s) 292
PasI CCCWGGG 1 cut(s) 120
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 111
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 7 cut(s) 287, 337, 692, 871, 945, 1065, 1141
Ppu21I YACGTR 2 cut(s) 14, 315
PshBI ATTAAT 2 cut(s) 645, 1035
PsiI TTATAA 1 cut(s) 1017
Psp124BI GAGCTC 1 cut(s) 820
Psp1406I AACGTT 2 cut(s) 387, 405
Psp6I CCWGG 2 cut(s) 119, 1119
PspGI CCWGG 2 cut(s) 119, 1119
PspN4I GGNNCC 1 cut(s) 853
PspPI GGNCC 2 cut(s) 464, 1117
PvuII CAGCTG 1 cut(s) 1058
RsaI GTAC 6 cut(s) 266, 293, 515, 743, 770, 1071
RsaNI GTAC 6 cut(s) 265, 292, 514, 742, 769, 1070
RseI CAYNNNNRTG 2 cut(s) 378, 482
SacI GAGCTC 1 cut(s) 820
SaqAI TTAA 4 cut(s) 255, 540, 645, 1035
SatI GCNGC 7 cut(s) 286, 336, 691, 870, 944, 1064, 1140
Sau3AI GATC 5 cut(s) 103, 162, 355, 649, 844
Sau96I GGNCC 2 cut(s) 464, 1117
ScaI AGTACT 1 cut(s) 515
ScrFI CCNGG 3 cut(s) 121, 301, 1121
SduI GDGCHC 1 cut(s) 820
SfaNI GCATC 1 cut(s) 355
SinI GGWCC 1 cut(s) 464
SmiMI CAYNNNNRTG 2 cut(s) 378, 482
SmlI CTYRAG 2 cut(s) 539, 617
SmoI CTYRAG 2 cut(s) 539, 617
SsiI CCGC 8 cut(s) 199, 213, 286, 676, 690, 777, 870, 908
SstI GAGCTC 1 cut(s) 820
StyD4I CCNGG 3 cut(s) 119, 299, 1119
StyI CCWWGG 3 cut(s) 87, 570, 772
TaaI ACNGT 2 cut(s) 584, 683
TaiI ACGT 4 cut(s) 16, 317, 390, 408
TaqI TCGA 3 cut(s) 672, 696, 1151
TatI WGTACW 1 cut(s) 513
TauI GCSGC 3 cut(s) 288, 693, 872
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 4 cut(s) 255, 540, 645, 1035
Tru9I TTAA 4 cut(s) 255, 540, 645, 1035
TscAI CASTG 2 cut(s) 385, 555
TseFI GTSAC 2 cut(s) 206, 1095
TseI GCWGC 4 cut(s) 335, 943, 1063, 1139
Tsp45I GTSAC 2 cut(s) 206, 1095
TspDTI ATGAA 1 cut(s) 147
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 2 cut(s) 385, 555
Vha464I CTTAAG 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 464
VspI ATTAAT 2 cut(s) 645, 1035
XagI CCTNNNNNAGG 1 cut(s) 1109
XapI RAATTY 3 cut(s) 134, 230, 889
XceI RCATGY 1 cut(s) 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 1083
ZrmI AGTACT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.