Rw4G006450

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000286
Physical Location & Seq
Forward (+)
13527201 .. 13529113
1913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006450.1

Sequence Viewer

Length: 669 bp
ATGGAAGAAATACGTAAGGCTGCTTTAGCTTACTACAATAACTTGCCAAAGGATCTACAGAAGCTGACATCTGCAAAATTCAAAGAAATTGATTCAAGTGGTGATGGCACAATAAGCATCGAGGAATTCAAGAAGAGTATGGGAAGCTCTTTCCACAACAACTCTGTGATCATGGAGAGAAGCTTCAAGGAGCTGGACAACAACGGAGACCGCAAGTTGGATTTCAACGAGTACATCACTCTTTACTATCTTGTAGAGAGCGGTAGGGTGCTGATTTATTGTGCGGGCAAGGGATGTAAGGCCGCACCTTTTCTCAAGGGACTCTATTTCACTTGCGTCGACTGCTTCCACAATAACAAAAATGGAACTTTCGATCTCTGCACCTCTTGCTACCACAATGCAGACTTTGATCATGAACACACAAACTTTGTGGATAACCATGTGTTACTTCGTTCCAAGGCTGCTTGTTGTGAATCCACATCTGAGCCATGCTCTCCTGGGTCTGATTCTGAGTCCAATCAAGTCAGTCGAACTGGATCAAAGGGAGTGCCAGTTAATCGATCTGGATCAAAGAGGAGGGCGGTACTTGGAGCAATCAAATCTGGAGCGAGTGTTGCACGTGCAGTAGGAAATGTGGCTTCTGATGTTGCTTCTTGCAGCATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

222

Amino Acids

24.29

Weight (kDa)

7.53

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 24 - 83 2.2e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 261
AccI GTMKAC 1 cut(s) 339
AciI CCGC 5 cut(s) 211, 261, 284, 303, 581
AclWI GGATC 3 cut(s) 60, 544, 574
AcsI RAATTY 2 cut(s) 77, 125
AcvI CACGTG 1 cut(s) 620
AfaI GTAC 2 cut(s) 233, 585
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 5 cut(s) 82, 96, 130, 187, 226
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 5 cut(s) 29, 64, 147, 183, 193
AluI AGCT 5 cut(s) 29, 64, 147, 183, 193
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 3 cut(s) 60, 544, 574
AlwNI CAGNNNCTG 1 cut(s) 64
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 3 cut(s) 20, 461, 657
ApoI RAATTY 2 cut(s) 77, 125
AsuHPI GGTGA 1 cut(s) 113
BbrPI CACGTG 1 cut(s) 620
BbvI GCAGC 2 cut(s) 7, 448
BccI CCATC 1 cut(s) 98
BciT130I CCWGG 1 cut(s) 498
BclI TGATCA 2 cut(s) 168, 409
BcoDI GTCTC 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 56
BisI GCNGC 4 cut(s) 21, 303, 462, 658
BlsI GCNGC 4 cut(s) 22, 304, 463, 659
Bme1390I CCNGG 1 cut(s) 498
BmrFI CCNGG 1 cut(s) 498
BmsI GCATC 1 cut(s) 126
BplI GAGNNNNNCTC 2 cut(s) 476, 508
BpmI CTGGAG 1 cut(s) 624
BpuEI CTTGAG 1 cut(s) 299
Bsa29I ATCGAT 1 cut(s) 559
BsaAI YACGTR 2 cut(s) 14, 620
BsaBI GATNNNNATC 1 cut(s) 565
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 2 cut(s) 456, 497
BsaXI ACNNNNNCTCC 4 cut(s) 537, 567, 597, 627
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse1I ACTGG 2 cut(s) 538, 551
Bse8I GATNNNNATC 1 cut(s) 565
BseBI CCWGG 1 cut(s) 498
BseCI ATCGAT 1 cut(s) 559
BseDI CCNNGG 2 cut(s) 456, 497
BseGI GGATG 1 cut(s) 299
BseJI GATNNNNATC 1 cut(s) 565
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMII CTCAG 2 cut(s) 474, 501
BseNI ACTGG 2 cut(s) 538, 551
BseRI GAGGAG 1 cut(s) 589
BseXI GCAGC 2 cut(s) 7, 448
BsgI GTGCAG 2 cut(s) 364, 642
BshFI GGCC 1 cut(s) 302
BshVI ATCGAT 1 cut(s) 559
BslFI GGGAC 1 cut(s) 333
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 333
BsnI GGCC 1 cut(s) 302
Bso31I GGTCTC 1 cut(s) 201
Bsp143I GATC 7 cut(s) 52, 168, 373, 409, 536, 560, 566
BspACI CCGC 5 cut(s) 211, 261, 284, 303, 581
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 2 cut(s) 475, 502
BspDI ATCGAT 1 cut(s) 559
BspHI TCATGA 1 cut(s) 412
BspPI GGATC 3 cut(s) 60, 544, 574
BspTNI GGTCTC 1 cut(s) 201
BsrBI CCGCTC 1 cut(s) 261
BsrI ACTGG 2 cut(s) 538, 551
BssECI CCNNGG 2 cut(s) 456, 497
BssMI GATC 7 cut(s) 52, 168, 373, 409, 536, 560, 566
BssT1I CCWWGG 1 cut(s) 456
Bst2UI CCWGG 1 cut(s) 498
Bst6I CTCTTC 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 387
BstBAI YACGTR 2 cut(s) 14, 620
BstC8I GCNNGC 1 cut(s) 286
BstDEI CTNAG 2 cut(s) 483, 510
BstF5I GGATG 1 cut(s) 299
BstKTI GATC 7 cut(s) 55, 171, 376, 412, 539, 563, 569
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 7 cut(s) 52, 168, 373, 409, 536, 560, 566
BstMWI GCNNNNNNNGC 5 cut(s) 26, 114, 342, 387, 614
BstNI CCWGG 1 cut(s) 498
BstSCI CCNGG 1 cut(s) 496
BstSFI CTRYAG 1 cut(s) 56
BstSNI TACGTA 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 7, 448
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
Bsu15I ATCGAT 1 cut(s) 559
BsuRI GGCC 1 cut(s) 302
BsuTUI ATCGAT 1 cut(s) 559
BtsCI GGATG 1 cut(s) 299
Cac8I GCNNGC 1 cut(s) 286
CaiI CAGNNNCTG 1 cut(s) 64
CciI TCATGA 1 cut(s) 412
ClaI ATCGAT 1 cut(s) 559
CseI GACGC 1 cut(s) 325
Csp6I GTAC 2 cut(s) 232, 584
CspCI CAANNNNNGTGG 2 cut(s) 411, 446
CviAII CATG 5 cut(s) 172, 413, 440, 489, 664
CviQI GTAC 2 cut(s) 232, 584
DdeI CTNAG 2 cut(s) 483, 510
DpnI GATC 7 cut(s) 54, 170, 375, 411, 538, 562, 568
DpnII GATC 7 cut(s) 52, 168, 373, 409, 536, 560, 566
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Eco105I TACGTA 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 201
Eco72I CACGTG 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 456
FaeI CATG 5 cut(s) 175, 416, 443, 492, 667
FaiI YATR 6 cut(s) 140, 173, 414, 441, 490, 665
FaqI GGGAC 1 cut(s) 333
FatI CATG 5 cut(s) 171, 412, 439, 488, 663
FauI CCCGC 1 cut(s) 277
FbaI TGATCA 2 cut(s) 168, 409
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 4 cut(s) 21, 303, 462, 658
FokI GGATG 1 cut(s) 306
Fsp4HI GCNGC 4 cut(s) 21, 303, 462, 658
GluI GCNGC 4 cut(s) 21, 303, 462, 658
GsuI CTGGAG 1 cut(s) 624
HaeIII GGCC 1 cut(s) 302
HgaI GACGC 1 cut(s) 325
Hin1II CATG 5 cut(s) 175, 416, 443, 492, 667
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 5 cut(s) 92, 321, 473, 506, 512
HphI GGTGA 1 cut(s) 113
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 4 cut(s) 484, 505, 511, 643
Hpy188III TCNNGA 4 cut(s) 130, 413, 564, 603
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 1 cut(s) 341
HpyCH4IV ACGT 2 cut(s) 13, 619
HpyCH4V TGCA 6 cut(s) 74, 381, 401, 617, 623, 657
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 114, 342, 387, 614
HpyF3I CTNAG 2 cut(s) 483, 510
HpySE526I ACGT 2 cut(s) 13, 619
Hsp92II CATG 5 cut(s) 175, 416, 443, 492, 667
Ksp22I TGATCA 2 cut(s) 168, 409
Kzo9I GATC 7 cut(s) 52, 168, 373, 409, 536, 560, 566
LmnI GCTCC 3 cut(s) 190, 590, 605
LpnPI CCDG 7 cut(s) 179, 483, 510, 519, 549, 564, 588
Lsp1109I GCAGC 2 cut(s) 7, 448
LweI GCATC 1 cut(s) 126
MaeII ACGT 2 cut(s) 13, 619
MaeIII GTNAC 1 cut(s) 444
MalI GATC 7 cut(s) 54, 170, 375, 411, 538, 562, 568
MbiI CCGCTC 1 cut(s) 261
MboI GATC 7 cut(s) 52, 168, 373, 409, 536, 560, 566
MboII GAAGA 2 cut(s) 17, 145
MflI RGATCY 1 cut(s) 52
MluCI AATT 3 cut(s) 77, 87, 125
MlyI GAGTC 2 cut(s) 315, 521
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 4 cut(s) 115, 394, 567, 570
MseI TTAA 1 cut(s) 555
MspR9I CCNGG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 498
MwoI GCNNNNNNNGC 5 cut(s) 26, 114, 342, 387, 614
NdeII GATC 7 cut(s) 52, 168, 373, 409, 536, 560, 566
NlaIII CATG 5 cut(s) 175, 416, 443, 492, 667
PagI TCATGA 1 cut(s) 412
PfeI GAWTC 3 cut(s) 92, 473, 506
PkrI GCNGC 4 cut(s) 22, 304, 463, 659
PleI GAGTC 2 cut(s) 315, 520
PmaCI CACGTG 1 cut(s) 620
PmlI CACGTG 1 cut(s) 620
PpsI GAGTC 2 cut(s) 315, 520
Ppu21I YACGTR 2 cut(s) 14, 620
Psp6I CCWGG 1 cut(s) 496
PspCI CACGTG 1 cut(s) 620
PspGI CCWGG 1 cut(s) 496
PstNI CAGNNNCTG 1 cut(s) 64
PsuI RGATCY 1 cut(s) 52
RsaI GTAC 2 cut(s) 233, 585
RsaNI GTAC 2 cut(s) 232, 584
SalI GTCGAC 1 cut(s) 338
SaqAI TTAA 1 cut(s) 555
SatI GCNGC 4 cut(s) 21, 303, 462, 658
Sau3AI GATC 7 cut(s) 52, 168, 373, 409, 536, 560, 566
SchI GAGTC 2 cut(s) 315, 521
ScrFI CCNGG 1 cut(s) 498
SetI ASST 9 cut(s) 16, 31, 66, 149, 185, 195, 310, 386, 622
SfaNI GCATC 1 cut(s) 126
SfcI CTRYAG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
SnaBI TACGTA 1 cut(s) 14
Sse9I AATT 3 cut(s) 77, 87, 125
SsiI CCGC 5 cut(s) 211, 261, 284, 303, 581
StyD4I CCNGG 1 cut(s) 496
StyI CCWWGG 1 cut(s) 456
TaiI ACGT 2 cut(s) 16, 622
TaqI TCGA 5 cut(s) 120, 339, 372, 529, 559
TasI AATT 3 cut(s) 77, 87, 125
TatI WGTACW 1 cut(s) 231
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 3 cut(s) 92, 473, 506
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TseI GCWGC 3 cut(s) 20, 461, 657
TspDTI ATGAA 1 cut(s) 429
TspGWI ACGGA 1 cut(s) 219
XapI RAATTY 2 cut(s) 77, 125
XmiI GTMKAC 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.