Rh4CG064400

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000286
Physical Location & Seq
Reverse (-)
12106840 .. 12118920
12081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG064400.1

Sequence Viewer

Length: 819 bp
ATGGGAGACATCAAAGAAATTCGTGTTGCTGCTTTAGCTTATTACCATAATTTTTGTGCAAAGGAACAGCATGAGGCGTATCTCTTTTTTCAAACAATGGATAAAGATGATGATGGAAAAATAACCATGGAGGACTTCAAGCAGAAGTTTGAGGGTTGTTTCGGCGACAATGTCAACCGTGCCTTCCAGGACCTAGACAAAAATGGAGATGGCAATTTGGATTTTGAGGAATCTATCATTCTTTACTACTGTCTTATGGCTGGGAGGATCATTATTTGCCATTGCTGCAAAGCCTTTTTTCTAAAGGGACTGTATTTCACTTGCGTGGACTGCTTCAAGCATAACCAAACTACAACTTTTGATCTTTGCATCTCCTGCTACGACAGCCGCGAGTACGCCTTCCTAAGTCACGAACACTGCAACTTTTTGGACAACTACGCCTTGCTAGCTACTTATAGACTTTCTGGATTGACAAACCAGCCAGCAAGCTCAGCAAAGTCGGTTGATCCTAAGAACTATCAAGCAGACCCGGCCTTGCCCAAGGGCAGCCCTGGCGATAGCCCAGGGCTTAAGGGCAGCCCTGGCGACAGCCCAGGGCTTAAGGGCAGCCCTGACGACAGCCCAGGGCCCAAGAGCAGCCCTGGGCCCAAGCCAGGGCCCAAGGAAAGGACTGACTCTGGGCACCAAACAAATTTTCGCTCATGGCATCAAGAATCTCAGAACCGGCCCGCCTGTCAATTCATTTCATCTAGAAGTGTAAGCTTGTTCTCTCATTGTTTCGTTCTACTTTTTTTTTATATCAATAACGAATGTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

272

Amino Acids

30.54

Weight (kDa)

5.78

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 681
AccII CGCG 1 cut(s) 390
AciI CCGC 2 cut(s) 388, 729
AclWI GGATC 2 cut(s) 275, 500
AcsI RAATTY 2 cut(s) 18, 691
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 3 cut(s) 653, 654, 666
AflII CTTAAG 2 cut(s) 569, 599
AgsI TTSAA 3 cut(s) 92, 139, 337
AjnI CCWGG 8 cut(s) 186, 550, 562, 580, 592, 622, 640, 652
AjuI GAANNNNNNNTTGG 2 cut(s) 678, 710
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 4 cut(s) 38, 449, 489, 762
AluI AGCT 4 cut(s) 38, 449, 489, 762
AlwI GGATC 2 cut(s) 275, 500
AoxI GGCC 5 cut(s) 531, 626, 644, 656, 725
ApaI GGGCCC 3 cut(s) 630, 648, 660
ApeKI GCWGC 6 cut(s) 29, 285, 546, 576, 606, 636
ApoI RAATTY 2 cut(s) 18, 691
AspS9I GGNCC 8 cut(s) 190, 626, 627, 644, 645, 656, 657, 726
AsuC2I CCSGG 1 cut(s) 530
AsuNHI GCTAGC 1 cut(s) 445
AvaII GGWCC 1 cut(s) 190
BaeGI GKGCMC 4 cut(s) 630, 648, 660, 684
BanI GGYRCC 1 cut(s) 681
BanII GRGCYC 3 cut(s) 630, 648, 660
BbvI GCAGC 6 cut(s) 16, 272, 558, 588, 618, 648
BccI CCATC 2 cut(s) 107, 203
BciT130I CCWGG 8 cut(s) 188, 552, 564, 582, 594, 624, 642, 654
BcnI CCSGG 1 cut(s) 530
BfaI CTAG 3 cut(s) 194, 446, 750
BfrI CTTAAG 2 cut(s) 569, 599
BisI GCNGC 7 cut(s) 30, 286, 388, 547, 577, 607, 637
BlpI GCTNAGC 1 cut(s) 490
BlsI GCNGC 7 cut(s) 31, 287, 389, 548, 578, 608, 638
Bme1390I CCNGG 9 cut(s) 188, 530, 552, 564, 582, 594, 624, 642, 654
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 8 cut(s) 190, 626, 627, 644, 645, 656, 657, 726
BmiI GGNNCC 4 cut(s) 628, 646, 658, 683
BmrFI CCNGG 9 cut(s) 188, 530, 552, 564, 582, 594, 624, 642, 654
BmsI GCATC 2 cut(s) 378, 715
BmtI GCTAGC 1 cut(s) 449
Bpu1102I GCTNAGC 1 cut(s) 490
BpuMI CCSGG 1 cut(s) 530
Bsc4I CCNNNNNNNGG 3 cut(s) 653, 654, 666
Bse118I RCCGGY 1 cut(s) 723
Bse3DI GCAATG 1 cut(s) 280
BseBI CCWGG 8 cut(s) 188, 552, 564, 582, 594, 624, 642, 654
BseLI CCNNNNNNNGG 3 cut(s) 653, 654, 666
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 2 cut(s) 504, 731
BseSI GKGCMC 4 cut(s) 630, 648, 660, 684
BseXI GCAGC 6 cut(s) 16, 272, 558, 588, 618, 648
BseYI CCCAGC 1 cut(s) 260
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 5 cut(s) 533, 628, 646, 658, 727
BshNI GGYRCC 1 cut(s) 681
BsiSI CCGG 2 cut(s) 530, 724
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 3 cut(s) 653, 654, 666
BsmFI GGGAC 1 cut(s) 321
BsnI GGCC 5 cut(s) 533, 628, 646, 658, 727
Bsp120I GGGCCC 3 cut(s) 626, 644, 656
Bsp1286I GDGCHC 4 cut(s) 630, 648, 660, 684
Bsp143I GATC 3 cut(s) 267, 361, 505
Bsp1720I GCTNAGC 1 cut(s) 490
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 2 cut(s) 388, 729
BspANI GGCC 5 cut(s) 533, 628, 646, 658, 727
BspCNI CTCAG 2 cut(s) 503, 730
BspFNI CGCG 1 cut(s) 390
BspLI GGNNCC 4 cut(s) 628, 646, 658, 683
BspOI GCTAGC 1 cut(s) 449
BspPI GGATC 2 cut(s) 275, 500
BspT107I GGYRCC 1 cut(s) 681
BspTI CTTAAG 2 cut(s) 569, 599
BsrDI GCAATG 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 723
BssAI RCCGGY 1 cut(s) 723
BssMI GATC 3 cut(s) 267, 361, 505
BssT1I CCWWGG 3 cut(s) 126, 540, 660
Bst2UI CCWGG 8 cut(s) 188, 552, 564, 582, 594, 624, 642, 654
Bst4CI ACNGT 3 cut(s) 179, 251, 312
BstAFI CTTAAG 2 cut(s) 569, 599
BstAPI GCANNNNNTGC 1 cut(s) 375
BstC8I GCNNGC 4 cut(s) 447, 483, 487, 729
BstDEI CTNAG 4 cut(s) 404, 490, 510, 717
BstDSI CCRYGG 1 cut(s) 126
BstFNI CGCG 1 cut(s) 390
BstKTI GATC 3 cut(s) 270, 364, 508
BstMBI GATC 3 cut(s) 267, 361, 505
BstNI CCWGG 8 cut(s) 188, 552, 564, 582, 594, 624, 642, 654
BstSCI CCNGG 9 cut(s) 186, 528, 550, 562, 580, 592, 622, 640, 652
BstSLI GKGCMC 4 cut(s) 630, 648, 660, 684
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 6 cut(s) 16, 272, 558, 588, 618, 648
BsuRI GGCC 5 cut(s) 533, 628, 646, 658, 727
BtgI CCRYGG 1 cut(s) 126
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 4 cut(s) 447, 483, 487, 729
Cfr10I RCCGGY 1 cut(s) 723
Cfr13I GGNCC 8 cut(s) 190, 626, 627, 644, 645, 656, 657, 726
Csp6I GTAC 1 cut(s) 394
CviAII CATG 3 cut(s) 71, 127, 702
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 4 cut(s) 404, 490, 510, 717
DpnI GATC 3 cut(s) 269, 363, 507
DpnII GATC 3 cut(s) 267, 361, 505
Eco130I CCWWGG 3 cut(s) 126, 540, 660
Eco24I GRGCYC 3 cut(s) 630, 648, 660
Eco47I GGWCC 1 cut(s) 190
EcoO109I RGGNCCY 3 cut(s) 190, 626, 656
EcoRII CCWGG 8 cut(s) 186, 550, 562, 580, 592, 622, 640, 652
EcoT14I CCWWGG 3 cut(s) 126, 540, 660
EcoT38I GRGCYC 3 cut(s) 630, 648, 660
ErhI CCWWGG 3 cut(s) 126, 540, 660
FaeI CATG 3 cut(s) 74, 130, 705
FaiI YATR 8 cut(s) 48, 72, 128, 257, 342, 456, 703, 798
FaqI GGGAC 1 cut(s) 321
FatI CATG 3 cut(s) 70, 126, 701
FauI CCCGC 1 cut(s) 736
Fnu4HI GCNGC 7 cut(s) 30, 286, 388, 547, 577, 607, 637
FriOI GRGCYC 3 cut(s) 630, 648, 660
Fsp4HI GCNGC 7 cut(s) 30, 286, 388, 547, 577, 607, 637
FspBI CTAG 3 cut(s) 194, 446, 750
GluI GCNGC 7 cut(s) 30, 286, 388, 547, 577, 607, 637
GsaI CCCAGC 1 cut(s) 264
HaeIII GGCC 5 cut(s) 533, 628, 646, 658, 727
HapII CCGG 2 cut(s) 530, 724
Hin1II CATG 3 cut(s) 74, 130, 705
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 760
HinfI GANTC 3 cut(s) 230, 674, 713
HpaII CCGG 2 cut(s) 530, 724
Hpy166II GTNNAC 2 cut(s) 175, 328
Hpy188I TCNGA 1 cut(s) 720
Hpy188III TCNNGA 4 cut(s) 410, 465, 710, 750
Hpy8I GTNNAC 2 cut(s) 175, 328
HpyAV CCTTC 2 cut(s) 193, 409
HpyCH4III ACNGT 3 cut(s) 179, 251, 312
HpyCH4V TGCA 4 cut(s) 59, 288, 369, 420
HpyF3I CTNAG 4 cut(s) 404, 490, 510, 717
Hsp92II CATG 3 cut(s) 74, 130, 705
Kzo9I GATC 3 cut(s) 267, 361, 505
Lsp1109I GCAGC 6 cut(s) 16, 272, 558, 588, 618, 648
LweI GCATC 2 cut(s) 378, 715
MaeI CTAG 3 cut(s) 194, 446, 750
MaeIII GTNAC 1 cut(s) 407
MalI GATC 3 cut(s) 269, 363, 507
MboI GATC 3 cut(s) 267, 361, 505
MhlI GDGCHC 4 cut(s) 630, 648, 660, 684
MluCI AATT 5 cut(s) 18, 49, 214, 691, 737
MlyI GAGTC 1 cut(s) 668
MnlI CCTC 5 cut(s) 67, 124, 145, 220, 258
MseI TTAA 2 cut(s) 570, 600
MslI CAYNNNNRTG 1 cut(s) 323
MspCI CTTAAG 2 cut(s) 569, 599
MspI CCGG 2 cut(s) 530, 724
MspR9I CCNGG 9 cut(s) 188, 530, 552, 564, 582, 594, 624, 642, 654
MvaI CCWGG 8 cut(s) 188, 552, 564, 582, 594, 624, 642, 654
MvnI CGCG 1 cut(s) 390
NciI CCSGG 1 cut(s) 530
NcoI CCATGG 1 cut(s) 126
NdeII GATC 3 cut(s) 267, 361, 505
NheI GCTAGC 1 cut(s) 445
NlaIII CATG 3 cut(s) 74, 130, 705
NlaIV GGNNCC 4 cut(s) 628, 646, 658, 683
NmuCI GTSAC 1 cut(s) 407
OliI CACNNNNGTG 1 cut(s) 323
PasI CCCWGGG 4 cut(s) 563, 593, 623, 641
PcsI WCGNNNNNNNCGW 1 cut(s) 387
PfeI GAWTC 2 cut(s) 230, 713
PflFI GACNNNGTC 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 7 cut(s) 31, 287, 389, 548, 578, 608, 638
PleI GAGTC 1 cut(s) 668
PpsI GAGTC 1 cut(s) 668
PpuMI RGGWCCY 1 cut(s) 190
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 8 cut(s) 186, 550, 562, 580, 592, 622, 640, 652
PspFI CCCAGC 1 cut(s) 260
PspGI CCWGG 8 cut(s) 186, 550, 562, 580, 592, 622, 640, 652
PspN4I GGNNCC 4 cut(s) 628, 646, 658, 683
PspOMI GGGCCC 3 cut(s) 626, 644, 656
PspPI GGNCC 8 cut(s) 190, 626, 627, 644, 645, 656, 657, 726
PspPPI RGGWCCY 1 cut(s) 190
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 2 cut(s) 570, 600
SatI GCNGC 7 cut(s) 30, 286, 388, 547, 577, 607, 637
Sau3AI GATC 3 cut(s) 267, 361, 505
Sau96I GGNCC 8 cut(s) 190, 626, 627, 644, 645, 656, 657, 726
SchI GAGTC 1 cut(s) 668
ScrFI CCNGG 9 cut(s) 188, 530, 552, 564, 582, 594, 624, 642, 654
SduI GDGCHC 4 cut(s) 630, 648, 660, 684
SetI ASST 5 cut(s) 40, 195, 451, 491, 764
SfaNI GCATC 2 cut(s) 378, 715
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 2 cut(s) 569, 599
SmoI CTYRAG 2 cut(s) 569, 599
Sse9I AATT 5 cut(s) 18, 49, 214, 691, 737
SsiI CCGC 2 cut(s) 388, 729
SspMI CTAG 3 cut(s) 194, 446, 750
StyD4I CCNGG 9 cut(s) 186, 528, 550, 562, 580, 592, 622, 640, 652
StyI CCWWGG 3 cut(s) 126, 540, 660
TaaI ACNGT 3 cut(s) 179, 251, 312
TasI AATT 5 cut(s) 18, 49, 214, 691, 737
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 2 cut(s) 230, 713
Tru1I TTAA 2 cut(s) 570, 600
Tru9I TTAA 2 cut(s) 570, 600
TscAI CASTG 1 cut(s) 422
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 6 cut(s) 29, 285, 546, 576, 606, 636
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 2 cut(s) 730, 735
TspRI CASTG 1 cut(s) 422
Tth111I GACNNNGTC 1 cut(s) 170
Vha464I CTTAAG 2 cut(s) 569, 599
VpaK11BI GGWCC 1 cut(s) 190
XapI RAATTY 2 cut(s) 18, 691
XbaI TCTAGA 1 cut(s) 749
XspI CTAG 3 cut(s) 194, 446, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.