FvH4_4g04760

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000286
Physical Location & Seq
Forward (+)
4139124 .. 4140998
1875 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04760.t1

Sequence Viewer

Length: 900 bp
ATGCGTGAAGGTGGCCATGGAGTATGTGATGGGCATGAATGTGAATCCCATATCGGCACTGGTGGACTGTATTTTGTTTGTGTTGATTGTTTTGATGATCCAAACAAAGAAGAAAGTTTCAATCTCTGTACCTCCTGTTACCGGCGTGGAAAGTACGCTCACCGCCACAACAACTTTTTGGACAACTTCGTCTTGTTGTGTTCCAAGTTTGTTCGTAAATCCACCGCACAGCTGGCCATAGTACCACAAACTCAGGTACCGGTCGAGATAGATCCAATGATGGAAAAAATACGTTCTACTGCGTTTGCATATTATGAAAATTTGTCATCGGACAACAAGCAGAAGGCACTTGAATTTATTAGAGAAATGAATATAGATGGTGTCAGCTGGGCAATAAGCCTCCAGCAATATGAGAACAAATTTGGAAGCTCCAGCAGGATGAAGAAGTGTTTCGAGGTGGTGGACAGAAACAGAGATGGCTGGTTGGATTTTCCCGAGTTCGTCACTCTTTACTATCTTCACAAGAGTGGGAGGCTGAGAGTTCGGTGTGATGGCCATGGATGTGATGAAGCCCCTTTCCTCAAGGGAGCTTTTTTTGTTTGCGTCGACTGCTTCAAGAAACATATTCATTTCTACTTGTGCACCTTCTGCTACCGCTATGGAAAGTACACTCACAGCCGCAGGCATACCAACTTTGAGGATAACTGCATGTTGCCGCAAGATATGATATTGCCTCCTTACTCACCCGATCAAACCCAAGCAGCAGTACCGAGTCAACCTGCAGCCACACAAAATTATCAGGGGGCGTTGGTCGTGGCCACTCAATCGGTCTCTCTACTGGGCAACATTATTAGCATTGGAGCTACAGTTGGGGGTTTCATTGGGTGCAACATTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

300

Amino Acids

33.94

Weight (kDa)

6.81

Isoelectric Point (pI)

51.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
Acc36I ACCTGC 1 cut(s) 787
Acc65I GGTACC 1 cut(s) 256
AccB1I GGYRCC 1 cut(s) 256
AccI GTMKAC 1 cut(s) 606
AciI CCGC 5 cut(s) 163, 225, 655, 679, 716
AclWI GGATC 2 cut(s) 92, 266
AcoI YGGCCR 4 cut(s) 13, 234, 553, 816
AcsI RAATTY 3 cut(s) 319, 353, 419
AfaI GTAC 6 cut(s) 130, 155, 243, 258, 668, 768
AfiI CCNNNNNNNGG 1 cut(s) 141
AgeI ACCGGT 1 cut(s) 259
AgsI TTSAA 3 cut(s) 121, 353, 616
AleI CACNNNNGTG 1 cut(s) 525
AluBI AGCT 5 cut(s) 232, 387, 429, 590, 863
AluI AGCT 5 cut(s) 232, 387, 429, 590, 863
Alw21I GWGCWC 1 cut(s) 644
Alw26I GTCTC 1 cut(s) 835
Alw44I GTGCAC 1 cut(s) 640
AlwI GGATC 2 cut(s) 92, 266
Ama87I CYCGRG 1 cut(s) 494
AoxI GGCC 4 cut(s) 13, 234, 553, 816
ApaLI GTGCAC 1 cut(s) 640
ApeKI GCWGC 2 cut(s) 761, 782
ApoI RAATTY 3 cut(s) 319, 353, 419
AsiGI ACCGGT 1 cut(s) 259
Asp700I GAANNNNTTC 1 cut(s) 449
Asp718I GGTACC 1 cut(s) 256
AsuHPI GGTGA 2 cut(s) 152, 735
AvaI CYCGRG 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 644
BaeI ACNNNNGTAYC 2 cut(s) 240, 273
BalI TGGCCA 4 cut(s) 15, 236, 555, 818
BanI GGYRCC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 644
BbvI GCAGC 2 cut(s) 773, 794
BccI CCATC 5 cut(s) 23, 274, 371, 470, 545
BcoDI GTCTC 1 cut(s) 835
BfmI CTRYAG 2 cut(s) 780, 864
BfuAI ACCTGC 1 cut(s) 787
BisI GCNGC 4 cut(s) 679, 716, 762, 783
BlsI GCNGC 4 cut(s) 680, 717, 763, 784
BmeT110I CYCGRG 1 cut(s) 494
BmiI GGNNCC 1 cut(s) 258
BmrI ACTGGG 1 cut(s) 848
BmuI ACTGGG 1 cut(s) 848
BpmI CTGGAG 2 cut(s) 386, 415
BpuEI CTTGAG 1 cut(s) 566
BsaI GGTCTC 1 cut(s) 835
BsaJI CCNNGG 2 cut(s) 16, 556
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 852, 882
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse118I RCCGGY 2 cut(s) 141, 259
Bse1I ACTGG 2 cut(s) 64, 843
BseDI CCNNGG 2 cut(s) 16, 556
BseGI GGATG 2 cut(s) 444, 566
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMII CTCAG 2 cut(s) 266, 527
BseNI ACTGG 2 cut(s) 64, 843
BseSI GKGCMC 1 cut(s) 644
BseXI GCAGC 2 cut(s) 773, 794
BseYI CCCAGC 1 cut(s) 387
Bsh1285I CGRYCG 1 cut(s) 264
BshFI GGCC 4 cut(s) 15, 236, 555, 818
BshNI GGYRCC 1 cut(s) 256
BshTI ACCGGT 1 cut(s) 259
BsiEI CGRYCG 1 cut(s) 264
BsiHKAI GWGCWC 1 cut(s) 644
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 2 cut(s) 142, 260
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 1 cut(s) 835
BsnI GGCC 4 cut(s) 15, 236, 555, 818
Bso31I GGTCTC 1 cut(s) 835
BsoBI CYCGRG 1 cut(s) 494
Bsp1286I GDGCHC 1 cut(s) 644
Bsp143I GATC 3 cut(s) 97, 271, 748
Bsp19I CCATGG 2 cut(s) 16, 556
BspACI CCGC 5 cut(s) 163, 225, 655, 679, 716
BspANI GGCC 4 cut(s) 15, 236, 555, 818
BspCNI CTCAG 2 cut(s) 265, 528
BspLI GGNNCC 1 cut(s) 258
BspMAI CTGCAG 1 cut(s) 784
BspMI ACCTGC 1 cut(s) 787
BspPI GGATC 2 cut(s) 92, 266
BspT107I GGYRCC 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 835
BsrFI RCCGGY 2 cut(s) 141, 259
BsrI ACTGG 2 cut(s) 64, 843
BssAI RCCGGY 2 cut(s) 141, 259
BssECI CCNNGG 2 cut(s) 16, 556
BssMI GATC 3 cut(s) 97, 271, 748
BssT1I CCWWGG 2 cut(s) 16, 556
Bst4CI ACNGT 2 cut(s) 69, 868
BstAPI GCANNNNNTGC 1 cut(s) 648
BstC8I GCNNGC 2 cut(s) 234, 683
BstDEI CTNAG 2 cut(s) 252, 536
BstDSI CCRYGG 2 cut(s) 16, 556
BstF5I GGATG 2 cut(s) 444, 566
BstKTI GATC 3 cut(s) 100, 274, 751
BstMAI GTCTC 1 cut(s) 835
BstMBI GATC 3 cut(s) 97, 271, 748
BstMCI CGRYCG 1 cut(s) 264
BstMWI GCNNNNNNNGC 3 cut(s) 233, 609, 648
BstNSI RCATGY 1 cut(s) 712
BstSFI CTRYAG 2 cut(s) 780, 864
BstSLI GKGCMC 1 cut(s) 644
BstV1I GCAGC 2 cut(s) 773, 794
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
BsuRI GGCC 4 cut(s) 15, 236, 555, 818
BtgI CCRYGG 2 cut(s) 16, 556
BtsCI GGATG 2 cut(s) 444, 566
BtsIMutI CAGTG 1 cut(s) 57
BveI ACCTGC 1 cut(s) 787
Cac8I GCNNGC 2 cut(s) 234, 683
Cfr10I RCCGGY 2 cut(s) 141, 259
CseI GACGC 1 cut(s) 592
Csp6I GTAC 6 cut(s) 129, 154, 242, 257, 667, 767
CspAI ACCGGT 1 cut(s) 259
CviAII CATG 4 cut(s) 17, 35, 557, 709
CviQI GTAC 6 cut(s) 129, 154, 242, 257, 667, 767
DdeI CTNAG 2 cut(s) 252, 536
DpnI GATC 3 cut(s) 99, 273, 750
DpnII GATC 3 cut(s) 97, 271, 748
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 4 cut(s) 13, 234, 553, 816
Eco130I CCWWGG 2 cut(s) 16, 556
Eco31I GGTCTC 1 cut(s) 835
Eco88I CYCGRG 1 cut(s) 494
EcoT14I CCWWGG 2 cut(s) 16, 556
ErhI CCWWGG 2 cut(s) 16, 556
FaeI CATG 4 cut(s) 20, 38, 560, 712
FatI CATG 4 cut(s) 16, 34, 556, 708
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 4 cut(s) 679, 716, 762, 783
FokI GGATG 2 cut(s) 451, 573
Fsp4HI GCNGC 4 cut(s) 679, 716, 762, 783
GluI GCNGC 4 cut(s) 679, 716, 762, 783
GsaI CCCAGC 1 cut(s) 391
GsuI CTGGAG 2 cut(s) 386, 415
HaeIII GGCC 4 cut(s) 15, 236, 555, 818
HapII CCGG 2 cut(s) 142, 260
HgaI GACGC 1 cut(s) 592
Hin1II CATG 4 cut(s) 20, 38, 560, 712
HincII GTYRAC 2 cut(s) 607, 776
HindII GTYRAC 2 cut(s) 607, 776
HinfI GANTC 2 cut(s) 44, 772
HpaII CCGG 2 cut(s) 142, 260
HphI GGTGA 2 cut(s) 152, 735
Hpy166II GTNNAC 6 cut(s) 65, 463, 607, 642, 669, 776
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 3 cut(s) 265, 494, 616
Hpy8I GTNNAC 6 cut(s) 65, 463, 607, 642, 669, 776
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 2 cut(s) 337, 655
HpyCH4III ACNGT 2 cut(s) 69, 868
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 5 cut(s) 308, 642, 708, 782, 888
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 609, 648
HpyF3I CTNAG 2 cut(s) 252, 536
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 4 cut(s) 20, 38, 560, 712
KpnI GGTACC 1 cut(s) 260
Kzo9I GATC 3 cut(s) 97, 271, 748
LmnI GCTCC 3 cut(s) 434, 587, 860
Lsp1109I GCAGC 2 cut(s) 773, 794
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 2 cut(s) 137, 502
MalI GATC 3 cut(s) 99, 273, 750
MboI GATC 3 cut(s) 97, 271, 748
MboII GAAGA 3 cut(s) 122, 454, 509
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 1 cut(s) 644
MlsI TGGCCA 4 cut(s) 15, 236, 555, 818
MluCI AATT 4 cut(s) 319, 353, 419, 793
MluNI TGGCCA 4 cut(s) 15, 236, 555, 818
MlyI GAGTC 1 cut(s) 781
MmeI TCCRAC 1 cut(s) 465
MnlI CCTC 7 cut(s) 142, 410, 448, 525, 590, 691, 744
Mox20I TGGCCA 4 cut(s) 15, 236, 555, 818
MroXI GAANNNNTTC 1 cut(s) 449
MscI TGGCCA 4 cut(s) 15, 236, 555, 818
MslI CAYNNNNRTG 3 cut(s) 39, 525, 561
Msp20I TGGCCA 4 cut(s) 15, 236, 555, 818
MspA1I CMGCKG 2 cut(s) 232, 387
MspI CCGG 2 cut(s) 142, 260
MwoI GCNNNNNNNGC 3 cut(s) 233, 609, 648
NcoI CCATGG 2 cut(s) 16, 556
NdeII GATC 3 cut(s) 97, 271, 748
NlaIII CATG 4 cut(s) 20, 38, 560, 712
NlaIV GGNNCC 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 502
NspI RCATGY 1 cut(s) 712
OliI CACNNNNGTG 1 cut(s) 525
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 1 cut(s) 44
PinAI ACCGGT 1 cut(s) 259
PkrI GCNGC 4 cut(s) 680, 717, 763, 784
PleI GAGTC 1 cut(s) 780
PpsI GAGTC 1 cut(s) 780
PspFI CCCAGC 1 cut(s) 387
PspN4I GGNNCC 1 cut(s) 258
PstI CTGCAG 1 cut(s) 784
PsuI RGATCY 1 cut(s) 271
PvuII CAGCTG 2 cut(s) 232, 387
RsaI GTAC 6 cut(s) 130, 155, 243, 258, 668, 768
RsaNI GTAC 6 cut(s) 129, 154, 242, 257, 667, 767
RseI CAYNNNNRTG 3 cut(s) 39, 525, 561
SalI GTCGAC 1 cut(s) 605
SatI GCNGC 4 cut(s) 679, 716, 762, 783
Sau3AI GATC 3 cut(s) 97, 271, 748
SchI GAGTC 1 cut(s) 781
SduI GDGCHC 1 cut(s) 644
SfcI CTRYAG 2 cut(s) 780, 864
SmiMI CAYNNNNRTG 3 cut(s) 39, 525, 561
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 4 cut(s) 319, 353, 419, 793
SsiI CCGC 5 cut(s) 163, 225, 655, 679, 716
StyI CCWWGG 2 cut(s) 16, 556
TaaI ACNGT 2 cut(s) 69, 868
TaiI ACGT 1 cut(s) 295
TaqI TCGA 3 cut(s) 264, 453, 606
TaqII GACCGA 1 cut(s) 817
TasI AATT 4 cut(s) 319, 353, 419, 793
TatI WGTACW 1 cut(s) 666
TauI GCSGC 2 cut(s) 681, 718
TfiI GAWTC 1 cut(s) 44
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 2 cut(s) 761, 782
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 7 cut(s) 51, 330, 383, 455, 582, 617, 868
TspRI CASTG 1 cut(s) 64
VneI GTGCAC 1 cut(s) 640
XapI RAATTY 3 cut(s) 319, 353, 419
XceI RCATGY 1 cut(s) 712
XcmI CCANNNNNNNNNTGG 2 cut(s) 56, 229
XmiI GTMKAC 1 cut(s) 606
XmnI GAANNNNTTC 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.