Rorug03G0354500

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000286
Physical Location & Seq
Reverse (-)
43757121 .. 43759769
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0354500.1

Sequence Viewer

Length: 486 bp
ATGGGGTTCGTTAACAGCGTTAAGAAGCTTCAACGAAGTGAAATCAGTTCCAAGCGTGACCGAGCTATCACTATGAGCGATGCCCGGGAGAGGTTTTACAATATTCATCTCCAGTATATACATGGTGTTTGCTCTAAAAACTATACAATGTTGTACTCCATATCAGATACACATGTCCAAGAAATTAAGATTAGTCCGGGGATCATGTTGTTATTGGTTAATATCATTTATGGTTATTCGTCTTTCCTTGTCAGCAAGGCACTTCTTGCAGAGTTTATGACAGCTCAAAAGAGAAGCAAACCATTTAAAGCTTTGGGATCAGAACATATGAATAAACCTTCCCCTGTCATTCCATCTAACCTTACAAGTTCTACTCTCCAAAGTCTCGAATTTCAGATATGGGAAAGAAGTAAGCAGCCTCTGCAGGGAGCTACTACCTCATGCACTTGCCTTGATCATATTGTTCAAATAAAGGATGGTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

161

Amino Acids

18.16

Weight (kDa)

9.43

Isoelectric Point (pI)

65.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 46 - 74 2.2e-09 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 209, 325
AcsI RAATTY 1 cut(s) 389
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 2 cut(s) 90, 425
AflIII ACRYGT 1 cut(s) 172
AgsI TTSAA 2 cut(s) 32, 467
AluBI AGCT 5 cut(s) 28, 65, 284, 311, 431
AluI AGCT 5 cut(s) 28, 65, 284, 311, 431
Alw26I GTCTC 1 cut(s) 389
AlwI GGATC 2 cut(s) 209, 325
AlwNI CAGNNNCTG 1 cut(s) 421
Ama87I CYCGRG 1 cut(s) 84
ApeKI GCWGC 1 cut(s) 415
ApoI RAATTY 1 cut(s) 389
AsuC2I CCSGG 3 cut(s) 85, 86, 198
AvaI CYCGRG 1 cut(s) 84
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BbvI GCAGC 1 cut(s) 427
BccI CCATC 2 cut(s) 361, 470
BclI TGATCA 1 cut(s) 454
BcnI CCSGG 3 cut(s) 85, 86, 198
BcoDI GTCTC 1 cut(s) 389
BfmI CTRYAG 1 cut(s) 422
BisI GCNGC 1 cut(s) 416
BlsI GCNGC 1 cut(s) 417
Bme1390I CCNGG 3 cut(s) 85, 86, 198
BmeT110I CYCGRG 1 cut(s) 84
BmrFI CCNGG 3 cut(s) 85, 86, 198
BmsI GCATC 1 cut(s) 70
BpmI CTGGAG 1 cut(s) 95
BpuMI CCSGG 3 cut(s) 85, 86, 198
BsaJI CCNNGG 2 cut(s) 84, 197
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 425
Bse1I ACTGG 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 84, 197
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 2 cut(s) 90, 425
BseNI ACTGG 1 cut(s) 112
BseXI GCAGC 1 cut(s) 427
BsiHKCI CYCGRG 1 cut(s) 84
BsiSI CCGG 2 cut(s) 85, 197
BslI CCNNNNNNNGG 2 cut(s) 90, 425
BsmAI GTCTC 1 cut(s) 389
BsoBI CYCGRG 1 cut(s) 84
Bsp143I GATC 3 cut(s) 201, 317, 454
BspMAI CTGCAG 1 cut(s) 426
BspPI GGATC 2 cut(s) 209, 325
BsrI ACTGG 1 cut(s) 112
BssECI CCNNGG 2 cut(s) 84, 197
BssMI GATC 3 cut(s) 201, 317, 454
BstAPI GCANNNNNTGC 2 cut(s) 266, 421
BstF5I GGATG 1 cut(s) 481
BstKTI GATC 3 cut(s) 204, 320, 457
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 3 cut(s) 201, 317, 454
BstMWI GCNNNNNNNGC 2 cut(s) 266, 421
BstNSI RCATGY 1 cut(s) 176
BstSCI CCNGG 3 cut(s) 83, 84, 196
BstSFI CTRYAG 1 cut(s) 422
BstV1I GCAGC 1 cut(s) 427
BtgZI GCGATG 1 cut(s) 93
BtsCI GGATG 1 cut(s) 481
CaiI CAGNNNCTG 1 cut(s) 421
Cfr9I CCCGGG 1 cut(s) 84
Csp6I GTAC 1 cut(s) 154
CviAII CATG 4 cut(s) 122, 173, 205, 441
CviJI RGCY 6 cut(s) 28, 65, 284, 311, 418, 431
CviKI_1 RGCY 6 cut(s) 28, 65, 284, 311, 418, 431
CviQI GTAC 1 cut(s) 154
DpnI GATC 3 cut(s) 203, 319, 456
DpnII GATC 3 cut(s) 201, 317, 454
DraI TTTAAA 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 84
FaeI CATG 4 cut(s) 125, 176, 208, 444
FatI CATG 4 cut(s) 121, 172, 204, 440
FauNDI CATATG 1 cut(s) 327
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 1 cut(s) 416
Fsp4HI GCNGC 1 cut(s) 416
GluI GCNGC 1 cut(s) 416
GsuI CTGGAG 1 cut(s) 95
HapII CCGG 2 cut(s) 85, 197
Hin1II CATG 4 cut(s) 125, 176, 208, 444
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HindIII AAGCTT 2 cut(s) 26, 309
HpaI GTTAAC 1 cut(s) 13
HpaII CCGG 2 cut(s) 85, 197
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 3 cut(s) 166, 322, 396
Hpy188III TCNNGA 1 cut(s) 386
Hpy8I GTNNAC 1 cut(s) 13
HpyAV CCTTC 1 cut(s) 348
HpyCH4V TGCA 4 cut(s) 269, 424, 444, 482
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 421
Hsp92II CATG 4 cut(s) 125, 176, 208, 444
Ksp22I TGATCA 1 cut(s) 454
KspAI GTTAAC 1 cut(s) 13
Kzo9I GATC 3 cut(s) 201, 317, 454
LmnI GCTCC 1 cut(s) 428
LpnPI CCDG 5 cut(s) 98, 125, 210, 357, 410
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 1 cut(s) 70
MaeIII GTNAC 1 cut(s) 56
MalI GATC 3 cut(s) 203, 319, 456
MboI GATC 3 cut(s) 201, 317, 454
MluCI AATT 2 cut(s) 183, 389
MnlI CCTC 3 cut(s) 84, 429, 448
MseI TTAA 5 cut(s) 12, 21, 186, 219, 306
MspI CCGG 2 cut(s) 85, 197
MspR9I CCNGG 3 cut(s) 85, 86, 198
MwoI GCNNNNNNNGC 2 cut(s) 266, 421
NciI CCSGG 3 cut(s) 85, 86, 198
NdeI CATATG 1 cut(s) 327
NdeII GATC 3 cut(s) 201, 317, 454
NlaIII CATG 4 cut(s) 125, 176, 208, 444
NmuCI GTSAC 1 cut(s) 56
NspI RCATGY 1 cut(s) 176
PciI ACATGT 1 cut(s) 172
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PkrI GCNGC 1 cut(s) 417
PscI ACATGT 1 cut(s) 172
PstI CTGCAG 1 cut(s) 426
PstNI CAGNNNCTG 1 cut(s) 421
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SaqAI TTAA 5 cut(s) 12, 21, 186, 219, 306
SatI GCNGC 1 cut(s) 416
Sau3AI GATC 3 cut(s) 201, 317, 454
ScrFI CCNGG 3 cut(s) 85, 86, 198
SetI ASST 9 cut(s) 30, 67, 95, 286, 313, 340, 363, 433, 440
SfaNI GCATC 1 cut(s) 70
SfcI CTRYAG 1 cut(s) 422
SmaI CCCGGG 1 cut(s) 86
Sse9I AATT 2 cut(s) 183, 389
SspI AATATT 1 cut(s) 103
StyD4I CCNGG 3 cut(s) 83, 84, 196
TaqI TCGA 1 cut(s) 387
TaqII GACCGA 1 cut(s) 75
TasI AATT 2 cut(s) 183, 389
TatI WGTACW 1 cut(s) 153
Tru1I TTAA 5 cut(s) 12, 21, 186, 219, 306
Tru9I TTAA 5 cut(s) 12, 21, 186, 219, 306
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 1 cut(s) 415
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 2 cut(s) 95, 344
TspMI CCCGGG 1 cut(s) 84
XapI RAATTY 1 cut(s) 389
XceI RCATGY 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XmaI CCCGGG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.