Rw4G004880

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000286
Physical Location & Seq
Reverse (-)
9598749 .. 9608530
9782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004880.1

Sequence Viewer

Length: 423 bp
ATGGAGGAGTTACGACAGACGACATTAGCATATTACCAGGATGCCCCAGAAGACATGCGGTCGGCAGTAGAACATTTTGTCCAGGAGATGGATCGTGATGCAAAGGACCGAGTGAGCCGGCAAGATTTCTTAGAATACATGAAGATGGATGAAGATTGCGCACATATGAGTAACCATGGGTTCTTTGATGTGCTGAAGAAGGAAGAGAGCGAGGAGCTGGATTTCATGGACTCAAAGGCACATGAGTCCATTGGAGCAAAAGATCTGAAAAAGAAGCTCCAGTCTAGCAGTGGCAGTAGAACAGTAACTACTAGAGAAGCAAAAGCTTCTTCTTCTTCTCCTAAACCACCGACACCGGAGCCAAGCAGTTCCACATCCAATGCACCGGAGGGAAGTAGCAAACAGATGACGAACATTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5

Protein Analysis

140

Amino Acids

15.87

Weight (kDa)

5.02

Isoelectric Point (pI)

73.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 160
AccB7I CCANNNNNTGG 1 cut(s) 88
AciI CCGC 1 cut(s) 58
AclWI GGATC 1 cut(s) 99
AcuI CTGAAG 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 88
AhdI GACNNNNNGTC 1 cut(s) 58
AjnI CCWGG 2 cut(s) 36, 81
AloI GAACNNNNNNTCC 2 cut(s) 63, 95
AluBI AGCT 3 cut(s) 217, 277, 326
AluI AGCT 3 cut(s) 217, 277, 326
AlwI GGATC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 106
AvaII GGWCC 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 57
BccI CCATC 2 cut(s) 82, 139
BciT130I CCWGG 2 cut(s) 38, 83
BfaI CTAG 2 cut(s) 285, 312
BglII AGATCT 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 38, 83
Bme18I GGWCC 1 cut(s) 106
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 2 cut(s) 38, 83
BmsI GCATC 2 cut(s) 31, 88
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 1 cut(s) 263
BsaJI CCNNGG 1 cut(s) 175
BsaWI WCCGGW 2 cut(s) 355, 385
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 1 cut(s) 280
BseBI CCWGG 2 cut(s) 38, 83
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 3 cut(s) 46, 154, 374
BseLI CCNNNNNNNGG 1 cut(s) 88
BseNI ACTGG 1 cut(s) 280
BseRI GAGGAG 2 cut(s) 20, 227
Bsh1285I CGRYCG 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 62
BsiSI CCGG 3 cut(s) 118, 356, 386
BslI CCNNNNNNNGG 1 cut(s) 88
Bsp143I GATC 2 cut(s) 91, 262
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 360
BspPI GGATC 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 117
BsrI ACTGG 1 cut(s) 280
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 2 cut(s) 91, 262
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 2 cut(s) 38, 83
Bst4CI ACNGT 1 cut(s) 304
Bst6I CTCTTC 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 130
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 3 cut(s) 46, 154, 374
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 2 cut(s) 94, 265
BstMBI GATC 2 cut(s) 91, 262
BstMCI CGRYCG 1 cut(s) 62
BstNI CCWGG 2 cut(s) 38, 83
BstNSI RCATGY 1 cut(s) 58
BstSCI CCNGG 2 cut(s) 36, 81
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 3 cut(s) 46, 154, 374
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 119
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 106
CviAII CATG 5 cut(s) 55, 139, 176, 226, 242
CviJI RGCY 5 cut(s) 117, 217, 277, 326, 361
CviKI_1 RGCY 5 cut(s) 117, 217, 277, 326, 361
DdeI CTNAG 1 cut(s) 130
DpnI GATC 2 cut(s) 93, 264
DpnII GATC 2 cut(s) 91, 262
DriI GACNNNNNGTC 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 198
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 36, 81
EcoT14I CCWWGG 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 5 cut(s) 58, 142, 179, 229, 245
FaiI YATR 8 cut(s) 31, 56, 140, 165, 167, 177, 227, 243
FatI CATG 5 cut(s) 54, 138, 175, 225, 241
FauNDI CATATG 1 cut(s) 165
FokI GGATG 3 cut(s) 53, 161, 361
FspBI CTAG 2 cut(s) 285, 312
FspI TGCGCA 1 cut(s) 160
GlaI GCGC 1 cut(s) 160
GsuI CTGGAG 1 cut(s) 263
HapII CCGG 3 cut(s) 118, 356, 386
HhaI GCGC 1 cut(s) 161
Hin1II CATG 5 cut(s) 58, 142, 179, 229, 245
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HindIII AAGCTT 1 cut(s) 324
HinfI GANTC 2 cut(s) 230, 245
HpaII CCGG 3 cut(s) 118, 356, 386
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 1 cut(s) 95
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4V TGCA 2 cut(s) 101, 383
HpyF3I CTNAG 1 cut(s) 130
Hsp92II CATG 5 cut(s) 58, 142, 179, 229, 245
HspAI GCGC 1 cut(s) 159
KroI GCCGGC 1 cut(s) 117
KroNI GCCGGC 1 cut(s) 119
Kzo9I GATC 2 cut(s) 91, 262
LmnI GCTCC 4 cut(s) 214, 254, 282, 358
LweI GCATC 2 cut(s) 31, 88
MaeI CTAG 2 cut(s) 285, 312
MaeIII GTNAC 3 cut(s) 9, 170, 304
MalI GATC 2 cut(s) 93, 264
MboI GATC 2 cut(s) 91, 262
MboII GAAGA 8 cut(s) 62, 154, 164, 208, 215, 321, 324, 327
MflI RGATCY 1 cut(s) 262
MlyI GAGTC 2 cut(s) 224, 254
MnlI CCTC 2 cut(s) 205, 382
MroNI GCCGGC 1 cut(s) 117
MslI CAYNNNNRTG 1 cut(s) 143
MspI CCGG 3 cut(s) 118, 356, 386
MspR9I CCNGG 2 cut(s) 38, 83
MvaI CCWGG 2 cut(s) 38, 83
NaeI GCCGGC 1 cut(s) 119
NcoI CCATGG 1 cut(s) 175
NdeI CATATG 1 cut(s) 165
NdeII GATC 2 cut(s) 91, 262
NgoMIV GCCGGC 1 cut(s) 117
NlaIII CATG 5 cut(s) 58, 142, 179, 229, 245
NlaIV GGNNCC 1 cut(s) 360
NsbI TGCGCA 1 cut(s) 160
NspI RCATGY 1 cut(s) 58
PdiI GCCGGC 1 cut(s) 119
PflMI CCANNNNNTGG 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 81
PleI GAGTC 2 cut(s) 224, 253
PpsI GAGTC 2 cut(s) 224, 253
Psp6I CCWGG 2 cut(s) 36, 81
PspGI CCWGG 2 cut(s) 36, 81
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 1 cut(s) 106
PsrI GAACNNNNNNTAC 2 cut(s) 292, 324
PsuI RGATCY 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 143
Sau3AI GATC 2 cut(s) 91, 262
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 2 cut(s) 224, 254
ScrFI CCNGG 2 cut(s) 38, 83
SetI ASST 3 cut(s) 219, 279, 328
SfaNI GCATC 2 cut(s) 31, 88
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 143
SsiI CCGC 1 cut(s) 58
SspMI CTAG 2 cut(s) 285, 312
StyD4I CCNGG 2 cut(s) 36, 81
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 304
TaqII GACCGA 1 cut(s) 123
TscAI CASTG 1 cut(s) 295
TspDTI ATGAA 3 cut(s) 155, 165, 214
TspRI CASTG 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 106
XceI RCATGY 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 1 cut(s) 287
XspI CTAG 2 cut(s) 285, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.