Rorug03G0355100

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000286
Physical Location & Seq
Forward (+)
43887827 .. 43889818
1992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0355100.1

Sequence Viewer

Length: 1056 bp
ATGGAGAAAGGAAAAATGGGAGGCAGGCCCCCAGAAATAAGGAAGGACTGGGTGACAAACCGGTGGGTGATATTCTCACCGGCCAGAGCCAAGCGACCTTCGGACTTCAAATCCAAATCACCGGCGACGCCAACCCAACAACAATATCAACAATCTTGTCCCTTTTGTCTCGGCAACGAGCACGACTGCGCTCCCGAAATCTTCCGGGTTCCACCCGACGACCCCAAATGGAATCTCCGAGTGATCCAGAACATGTACCCGGCGCTGAGCCGTGCGGATGTCCCCGTCCCTGTCCCTGCCCAGGAGGAGTCCTACTCCTCCTTGGGTCAGGTCATGACTGGGTTCGGGTTCCACGACGTGGTGGTTGAGACCCCGCTTCACTCTGTTCAGCTCTCCGATCTTTCCCCAACTCAGATCGCCCATGTTCTTCGGGCCTATACCACCAGGATCCAACACTTGATCACTAATCATTCCATCAAATACGTCCAGGTGTTCAAAAACCATGGTGCTTCAGCTGGGGCATCAATGAGTCACTCTCACAGTCAGATGATGGCTCTGCCTATTGTTCCTCCTGCTGTTGCTGCTCGACTTGACTCTATGAAGGACTATTTTAATCGGACTGGCAAATGTAGTATCTGTGAATTCAACTCGAACGATCTTTTGATCCATGCATCGCCCCATTTTATTTCAATTGTACCTTATGCTGCTACATTTCCCTTCGAGATGTGGATCATTCCTCTCACTCACTCTCCTCATTTCCATGAACTTGATGCTGACAAGGCAGTTGACCTTGGGGGGATCCTGAAACTCATGCTTCGGAAGATGGCTTTGCAGTTGAATAACCCACCATTTAATTTCATGATTCAGACTTCTCCTCTTCAGGACAGTGCTTCAGAGTTGCCTCACTCCCATTGGTTCTTGCAAATAATACCCCAGTTAAGTGTTGCAGCTGGTTTTGAAATTGGAACTGGCTGTTACATAAATCCAGTCTTCCCAGAGGATGCAGCAAAAGTTTTGAGGGAAGTAAATGTTTTACCTTTGGACGGAGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

351

Amino Acids

39.2

Weight (kDa)

6.45

Isoelectric Point (pI)

52.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GalP_UDP_transf PF01087 14 - 190 7.4e-18 Galactose-1-phosphate uridyl transferase, N-terminal domain
DUF4921 PF16268 147 - 335 1.3e-08 Domain of unknown function (DUF4921)
GalP_UDP_tr_C PF02744 199 - 341 1.2e-06 Galactose-1-phosphate uridyl transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 358
AciI CCGC 2 cut(s) 275, 374
AclWI GGATC 7 cut(s) 238, 442, 455, 658, 737, 793, 806
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 3 cut(s) 495, 863, 876
AcyI GRCGYC 1 cut(s) 128
AdeI CACNNNGTG 1 cut(s) 358
AfaI GTAC 2 cut(s) 257, 696
AfiI CCNNNNNNNGG 3 cut(s) 301, 358, 1043
AflIII ACRYGT 1 cut(s) 252
AgeI ACCGGT 1 cut(s) 60
AgsI TTSAA 6 cut(s) 109, 496, 646, 690, 838, 959
AjiI CACGTC 1 cut(s) 358
AjnI CCWGG 3 cut(s) 300, 443, 486
AluBI AGCT 3 cut(s) 391, 515, 950
AluI AGCT 3 cut(s) 391, 515, 950
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 2 cut(s) 173, 362
AlwI GGATC 7 cut(s) 238, 442, 455, 658, 737, 793, 806
AoxI GGCC 3 cut(s) 26, 81, 432
ApeKI GCWGC 4 cut(s) 581, 704, 947, 1004
ApoI RAATTY 1 cut(s) 641
AsiGI ACCGGT 1 cut(s) 60
AspLEI GCGC 2 cut(s) 191, 265
AspS9I GGNCC 2 cut(s) 27, 432
AsuC2I CCSGG 2 cut(s) 206, 260
AsuHPI GGTGA 4 cut(s) 64, 69, 79, 111
BamHI GGATCC 2 cut(s) 447, 798
BbsI GAAGAC 1 cut(s) 982
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 4 cut(s) 568, 691, 959, 1016
BccI CCATC 3 cut(s) 482, 544, 817
BceAI ACGGC 1 cut(s) 255
BciT130I CCWGG 3 cut(s) 302, 445, 488
BclI TGATCA 1 cut(s) 459
BcnI CCSGG 2 cut(s) 206, 260
BcoDI GTCTC 2 cut(s) 173, 362
BfoI RGCGCY 1 cut(s) 266
BisI GCNGC 4 cut(s) 582, 705, 948, 1005
BlpI GCTNAGC 1 cut(s) 266
BlsI GCNGC 4 cut(s) 583, 706, 949, 1006
Bme1390I CCNGG 5 cut(s) 206, 260, 302, 445, 488
BmgBI CACGTC 1 cut(s) 358
BmgT120I GGNCC 2 cut(s) 27, 432
BmiI GGNNCC 5 cut(s) 29, 210, 350, 449, 800
BmrFI CCNGG 5 cut(s) 206, 260, 302, 445, 488
BmrI ACTGGG 3 cut(s) 58, 348, 928
BmsI GCATC 4 cut(s) 530, 680, 760, 991
BmuI ACTGGG 3 cut(s) 58, 348, 928
BpiI GAAGAC 1 cut(s) 982
BplI GAGNNNNNCTC 4 cut(s) 299, 331, 520, 552
Bpu1102I GCTNAGC 1 cut(s) 266
BpuMI CCSGG 2 cut(s) 206, 260
BsaBI GATNNNNATC 1 cut(s) 728
BsaHI GRCGYC 1 cut(s) 128
BsaI GGTCTC 1 cut(s) 362
BsaJI CCNNGG 4 cut(s) 300, 321, 502, 790
BsaWI WCCGGW 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 358, 1043
Bse118I RCCGGY 3 cut(s) 60, 79, 121
Bse1I ACTGG 6 cut(s) 53, 343, 625, 934, 973, 986
Bse8I GATNNNNATC 1 cut(s) 728
BseBI CCWGG 3 cut(s) 302, 445, 488
BseDI CCNNGG 4 cut(s) 300, 321, 502, 790
BseGI GGATG 2 cut(s) 283, 1006
BseJI GATNNNNATC 1 cut(s) 728
BseLI CCNNNNNNNGG 3 cut(s) 301, 358, 1043
BseMII CTCAG 2 cut(s) 257, 425
BseNI ACTGG 6 cut(s) 53, 343, 625, 934, 973, 986
BseRI GAGGAG 4 cut(s) 307, 320, 741, 864
BseXI GCAGC 4 cut(s) 568, 691, 959, 1016
BseYI CCCAGC 1 cut(s) 515
BshFI GGCC 3 cut(s) 28, 83, 434
BshTI ACCGGT 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 5 cut(s) 61, 80, 122, 205, 260
BslFI GGGAC 4 cut(s) 144, 266, 272, 278
BslI CCNNNNNNNGG 3 cut(s) 301, 358, 1043
BsmAI GTCTC 2 cut(s) 173, 362
BsmFI GGGAC 4 cut(s) 144, 266, 272, 278
BsnI GGCC 3 cut(s) 28, 83, 434
Bso31I GGTCTC 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 9 cut(s) 243, 397, 414, 447, 459, 655, 663, 729, 798
Bsp1720I GCTNAGC 1 cut(s) 266
Bsp19I CCATGG 1 cut(s) 502
BspACI CCGC 2 cut(s) 275, 374
BspANI GGCC 3 cut(s) 28, 83, 434
BspCNI CTCAG 2 cut(s) 258, 424
BspHI TCATGA 2 cut(s) 333, 858
BspLI GGNNCC 5 cut(s) 29, 210, 350, 449, 800
BspPI GGATC 7 cut(s) 238, 442, 455, 658, 737, 793, 806
BspTNI GGTCTC 1 cut(s) 362
BsrFI RCCGGY 3 cut(s) 60, 79, 121
BsrI ACTGG 6 cut(s) 53, 343, 625, 934, 973, 986
BssAI RCCGGY 3 cut(s) 60, 79, 121
BssECI CCNNGG 4 cut(s) 300, 321, 502, 790
BssMI GATC 9 cut(s) 243, 397, 414, 447, 459, 655, 663, 729, 798
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 3 cut(s) 321, 502, 790
Bst2UI CCWGG 3 cut(s) 302, 445, 488
Bst4CI ACNGT 2 cut(s) 542, 887
Bst6I CTCTTC 1 cut(s) 882
BstACI GRCGYC 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 2 cut(s) 266, 411
BstDSI CCRYGG 1 cut(s) 502
BstF5I GGATG 2 cut(s) 283, 1006
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 2 cut(s) 191, 265
BstKTI GATC 9 cut(s) 246, 400, 417, 450, 462, 658, 666, 732, 801
BstMAI GTCTC 2 cut(s) 173, 362
BstMBI GATC 9 cut(s) 243, 397, 414, 447, 459, 655, 663, 729, 798
BstMWI GCNNNNNNNGC 2 cut(s) 581, 779
BstNI CCWGG 3 cut(s) 302, 445, 488
BstNSI RCATGY 1 cut(s) 256
BstSCI CCNGG 5 cut(s) 204, 258, 300, 443, 486
BstV1I GCAGC 4 cut(s) 568, 691, 959, 1016
BstV2I GAAGAC 1 cut(s) 982
BstX2I RGATCY 2 cut(s) 447, 798
BstYI RGATCY 2 cut(s) 447, 798
BsuRI GGCC 3 cut(s) 28, 83, 434
BtgI CCRYGG 1 cut(s) 502
BtgZI GCGATG 1 cut(s) 657
BtrI CACGTC 1 cut(s) 358
BtsCI GGATG 2 cut(s) 283, 1006
BtsIMutI CAGTG 1 cut(s) 892
Cac8I GCNNGC 1 cut(s) 26
CciI TCATGA 2 cut(s) 333, 858
CfoI GCGC 2 cut(s) 191, 265
Cfr10I RCCGGY 3 cut(s) 60, 79, 121
Cfr13I GGNCC 2 cut(s) 27, 432
CseI GACGC 1 cut(s) 136
Csp6I GTAC 2 cut(s) 256, 695
CspAI ACCGGT 1 cut(s) 60
CviAII CATG 8 cut(s) 253, 334, 422, 503, 668, 761, 811, 859
CviQI GTAC 2 cut(s) 256, 695
DdeI CTNAG 2 cut(s) 266, 411
DpnI GATC 9 cut(s) 245, 399, 416, 449, 461, 657, 665, 731, 800
DpnII GATC 9 cut(s) 243, 397, 414, 447, 459, 655, 663, 729, 798
DraIII CACNNNGTG 1 cut(s) 358
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 882
EarI CTCTTC 1 cut(s) 882
Eco130I CCWWGG 3 cut(s) 321, 502, 790
Eco31I GGTCTC 1 cut(s) 362
Eco57I CTGAAG 3 cut(s) 495, 863, 876
EcoO109I RGGNCCY 1 cut(s) 27
EcoRI GAATTC 1 cut(s) 641
EcoRII CCWGG 3 cut(s) 300, 443, 486
EcoT14I CCWWGG 3 cut(s) 321, 502, 790
EcoT22I ATGCAT 1 cut(s) 673
ErhI CCWWGG 3 cut(s) 321, 502, 790
FaeI CATG 8 cut(s) 256, 337, 425, 506, 671, 764, 814, 862
FaqI GGGAC 4 cut(s) 144, 266, 272, 278
FatI CATG 8 cut(s) 252, 333, 421, 502, 667, 760, 810, 858
FauI CCCGC 1 cut(s) 381
FbaI TGATCA 1 cut(s) 459
Fnu4HI GCNGC 4 cut(s) 582, 705, 948, 1005
FokI GGATG 2 cut(s) 290, 1013
Fsp4HI GCNGC 4 cut(s) 582, 705, 948, 1005
GlaI GCGC 2 cut(s) 190, 264
GluI GCNGC 4 cut(s) 582, 705, 948, 1005
GsaI CCCAGC 1 cut(s) 519
HaeII RGCGCY 1 cut(s) 266
HaeIII GGCC 3 cut(s) 28, 83, 434
HapII CCGG 5 cut(s) 61, 80, 122, 205, 260
HgaI GACGC 1 cut(s) 136
HhaI GCGC 2 cut(s) 191, 265
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 8 cut(s) 256, 337, 425, 506, 671, 764, 814, 862
Hin6I GCGC 2 cut(s) 189, 263
HinP1I GCGC 2 cut(s) 189, 263
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HinfI GANTC 5 cut(s) 232, 308, 529, 593, 862
HpaII CCGG 5 cut(s) 61, 80, 122, 205, 260
HphI GGTGA 4 cut(s) 64, 69, 79, 111
Hpy166II GTNNAC 1 cut(s) 787
Hpy188I TCNGA 9 cut(s) 103, 239, 397, 414, 546, 618, 819, 867, 895
Hpy188III TCNNGA 7 cut(s) 194, 247, 334, 721, 802, 859, 881
Hpy8I GTNNAC 1 cut(s) 787
Hpy99I CGWCG 3 cut(s) 130, 221, 359
HpyAV CCTTC 4 cut(s) 37, 108, 595, 727
HpyCH4III ACNGT 2 cut(s) 542, 887
HpyCH4IV ACGT 2 cut(s) 357, 483
HpyCH4V TGCA 5 cut(s) 671, 832, 922, 947, 1004
HpyF10VI GCNNNNNNNGC 2 cut(s) 581, 779
HpyF3I CTNAG 2 cut(s) 266, 411
HpySE526I ACGT 2 cut(s) 357, 483
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 8 cut(s) 256, 337, 425, 506, 671, 764, 814, 862
HspAI GCGC 2 cut(s) 189, 263
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 9 cut(s) 243, 397, 414, 447, 459, 655, 663, 729, 798
LmnI GCTCC 1 cut(s) 196
Lsp1109I GCAGC 4 cut(s) 568, 691, 959, 1016
LweI GCATC 4 cut(s) 530, 680, 760, 991
MaeII ACGT 2 cut(s) 357, 483
MaeIII GTNAC 3 cut(s) 52, 530, 974
MalI GATC 9 cut(s) 245, 399, 416, 449, 461, 657, 665, 731, 800
MboI GATC 9 cut(s) 243, 397, 414, 447, 459, 655, 663, 729, 798
MboII GAAGA 5 cut(s) 193, 419, 832, 869, 982
MfeI CAATTG 1 cut(s) 690
MflI RGATCY 2 cut(s) 447, 798
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 4 cut(s) 641, 690, 853, 960
MlyI GAGTC 3 cut(s) 317, 538, 587
MmeI TCCRAC 1 cut(s) 475
Mph1103I ATGCAT 1 cut(s) 673
MseI TTAA 3 cut(s) 612, 852, 938
MslI CAYNNNNRTG 1 cut(s) 759
MspA1I CMGCKG 2 cut(s) 515, 950
MspI CCGG 5 cut(s) 61, 80, 122, 205, 260
MspR9I CCNGG 5 cut(s) 206, 260, 302, 445, 488
MunI CAATTG 1 cut(s) 690
MvaI CCWGG 3 cut(s) 302, 445, 488
MwoI GCNNNNNNNGC 2 cut(s) 581, 779
NciI CCSGG 2 cut(s) 206, 260
NcoI CCATGG 1 cut(s) 502
NdeII GATC 9 cut(s) 243, 397, 414, 447, 459, 655, 663, 729, 798
NlaIII CATG 8 cut(s) 256, 337, 425, 506, 671, 764, 814, 862
NlaIV GGNNCC 5 cut(s) 29, 210, 350, 449, 800
NmeAIII GCCGAG 1 cut(s) 150
NmuCI GTSAC 2 cut(s) 52, 530
NsiI ATGCAT 1 cut(s) 673
NspI RCATGY 1 cut(s) 256
PagI TCATGA 2 cut(s) 333, 858
PciI ACATGT 1 cut(s) 252
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PfeI GAWTC 2 cut(s) 232, 862
PflMI CCANNNNNTGG 1 cut(s) 358
PinAI ACCGGT 1 cut(s) 60
PkrI GCNGC 4 cut(s) 583, 706, 949, 1006
PleI GAGTC 3 cut(s) 316, 537, 587
PpsI GAGTC 3 cut(s) 316, 537, 587
PscI ACATGT 1 cut(s) 252
Psp6I CCWGG 3 cut(s) 300, 443, 486
PspFI CCCAGC 1 cut(s) 515
PspGI CCWGG 3 cut(s) 300, 443, 486
PspN4I GGNNCC 5 cut(s) 29, 210, 350, 449, 800
PspPI GGNCC 2 cut(s) 27, 432
PsuI RGATCY 2 cut(s) 447, 798
PvuII CAGCTG 2 cut(s) 515, 950
RsaI GTAC 2 cut(s) 257, 696
RsaNI GTAC 2 cut(s) 256, 695
RseI CAYNNNNRTG 1 cut(s) 759
SaqAI TTAA 3 cut(s) 612, 852, 938
SatI GCNGC 4 cut(s) 582, 705, 948, 1005
Sau3AI GATC 9 cut(s) 243, 397, 414, 447, 459, 655, 663, 729, 798
Sau96I GGNCC 2 cut(s) 27, 432
SchI GAGTC 3 cut(s) 317, 538, 587
ScrFI CCNGG 5 cut(s) 206, 260, 302, 445, 488
SduI GDGCHC 1 cut(s) 183
SfaNI GCATC 4 cut(s) 530, 680, 760, 991
SgrAI CRCCGGYG 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 759
Sse9I AATT 4 cut(s) 641, 690, 853, 960
SsiI CCGC 2 cut(s) 275, 374
StyD4I CCNGG 5 cut(s) 204, 258, 300, 443, 486
StyI CCWWGG 3 cut(s) 321, 502, 790
TaaI ACNGT 2 cut(s) 542, 887
TaiI ACGT 2 cut(s) 360, 486
TaqI TCGA 3 cut(s) 586, 650, 720
TasI AATT 4 cut(s) 641, 690, 853, 960
TfiI GAWTC 2 cut(s) 232, 862
Tru1I TTAA 3 cut(s) 612, 852, 938
Tru9I TTAA 3 cut(s) 612, 852, 938
TscAI CASTG 1 cut(s) 892
TseFI GTSAC 2 cut(s) 52, 530
TseI GCWGC 4 cut(s) 581, 704, 947, 1004
Tsp45I GTSAC 2 cut(s) 52, 530
TspDTI ATGAA 3 cut(s) 614, 777, 847
TspRI CASTG 1 cut(s) 892
Van91I CCANNNNNTGG 1 cut(s) 358
XapI RAATTY 1 cut(s) 641
XceI RCATGY 1 cut(s) 256
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.