RLG00000009612

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000286
Physical Location & Seq
Reverse (-)
55349411 .. 55359936
10526 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009612

Sequence Viewer

Length: 330 bp
ATGGAAGAAATACGTGAGGCTGCTTTAGCTTACTACCATAACTTGCCGGAGGACCTACAGAAGCCGGCATCTGCAAAATTCAAAGAAATTGATTCAAATGGTAATGGCACAATAAGCATCAGGGAATTCAAGAAGAGTATGGGAAGCTCTTTCGACAATGACTCCATGATCATGAAGAGAAGCTTCAAGGAGCTGGACAAGAACGGAGACGGCAATCGGTCTGGATCAAAGAGAGCGCAAGTTGAGCAATCTGGAACAAAGCGGGTGACTGGGTTAATATGCTCAACATCTGAGGGCGCGCAGTATTTGGAGCAATCAAATCTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

110

Amino Acids

12.13

Weight (kDa)

5.9

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 26 - 71 2.5e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 299
AciI CCGC 1 cut(s) 262
AclWI GGATC 1 cut(s) 232
AcsI RAATTY 2 cut(s) 77, 125
AgsI TTSAA 4 cut(s) 82, 96, 130, 187
AluBI AGCT 4 cut(s) 29, 147, 183, 193
AluI AGCT 4 cut(s) 29, 147, 183, 193
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 1 cut(s) 232
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 2 cut(s) 77, 125
AspLEI GCGC 3 cut(s) 238, 299, 301
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 277
AvaII GGWCC 1 cut(s) 52
BbvI GCAGC 1 cut(s) 7
BceAI ACGGC 1 cut(s) 226
BclI TGATCA 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 56
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmrI ACTGGG 1 cut(s) 279
BmsI GCATC 2 cut(s) 77, 126
BmuI ACTGGG 1 cut(s) 279
BsaAI YACGTR 1 cut(s) 14
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 274
BsePI GCGCGC 1 cut(s) 297
BseXI GCAGC 1 cut(s) 7
Bsh1236I CGCG 1 cut(s) 299
BsiSI CCGG 2 cut(s) 47, 65
BsmAI GTCTC 1 cut(s) 201
BsmBI CGTCTC 1 cut(s) 201
Bsp143I GATC 2 cut(s) 168, 224
BspACI CCGC 1 cut(s) 262
BspCNI CTCAG 1 cut(s) 283
BspFNI CGCG 1 cut(s) 299
BspHI TCATGA 1 cut(s) 171
BspPI GGATC 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 1 cut(s) 274
BssAI RCCGGY 1 cut(s) 64
BssHII GCGCGC 1 cut(s) 297
BssMI GATC 2 cut(s) 168, 224
Bst6I CTCTTC 2 cut(s) 128, 170
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 66, 299
BstDEI CTNAG 1 cut(s) 291
BstFNI CGCG 1 cut(s) 299
BstHHI GCGC 3 cut(s) 238, 299, 301
BstKTI GATC 2 cut(s) 171, 227
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 2 cut(s) 168, 224
BstMWI GCNNNNNNNGC 3 cut(s) 26, 114, 244
BstSFI CTRYAG 1 cut(s) 56
BstUI CGCG 1 cut(s) 299
BstV1I GCAGC 1 cut(s) 7
Cac8I GCNNGC 2 cut(s) 66, 299
CciI TCATGA 1 cut(s) 171
CfoI GCGC 3 cut(s) 238, 299, 301
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 1 cut(s) 52
CviAII CATG 2 cut(s) 166, 172
CviJI RGCY 6 cut(s) 20, 29, 64, 147, 183, 193
CviKI_1 RGCY 6 cut(s) 20, 29, 64, 147, 183, 193
DdeI CTNAG 1 cut(s) 291
DpnI GATC 2 cut(s) 170, 226
DpnII GATC 2 cut(s) 168, 224
Eam1104I CTCTTC 2 cut(s) 128, 170
EarI CTCTTC 2 cut(s) 128, 170
Eco47I GGWCC 1 cut(s) 52
EcoO109I RGGNCCY 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 125
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 2 cut(s) 169, 175
FaiI YATR 5 cut(s) 39, 140, 167, 173, 280
FalI AAGNNNNNCTT 2 cut(s) 167, 199
FatI CATG 2 cut(s) 165, 171
FauI CCCGC 1 cut(s) 255
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 1 cut(s) 21
Fsp4HI GCNGC 1 cut(s) 21
GlaI GCGC 3 cut(s) 237, 298, 300
GluI GCNGC 1 cut(s) 21
HapII CCGG 2 cut(s) 47, 65
HhaI GCGC 3 cut(s) 238, 299, 301
Hin1II CATG 2 cut(s) 169, 175
Hin6I GCGC 3 cut(s) 236, 297, 299
HinP1I GCGC 3 cut(s) 236, 297, 299
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 2 cut(s) 92, 161
HpaII CCGG 2 cut(s) 47, 65
HphI GGTGA 1 cut(s) 277
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 5 cut(s) 130, 172, 222, 252, 323
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 1 cut(s) 74
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 114, 244
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 2 cut(s) 169, 175
HspAI GCGC 3 cut(s) 236, 297, 299
KroI GCCGGC 1 cut(s) 64
KroNI GCCGGC 1 cut(s) 66
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 2 cut(s) 168, 224
LmnI GCTCC 2 cut(s) 190, 310
LpnPI CCDG 8 cut(s) 60, 78, 106, 179, 207, 237, 255, 308
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 2 cut(s) 77, 126
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 1 cut(s) 265
MalI GATC 2 cut(s) 170, 226
MboI GATC 2 cut(s) 168, 224
MboII GAAGA 3 cut(s) 17, 145, 187
MluCI AATT 3 cut(s) 77, 87, 125
MlyI GAGTC 1 cut(s) 155
MnlI CCTC 3 cut(s) 10, 43, 286
MroNI GCCGGC 1 cut(s) 64
MseI TTAA 1 cut(s) 275
MslI CAYNNNNRTG 1 cut(s) 170
MspI CCGG 2 cut(s) 47, 65
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 3 cut(s) 26, 114, 244
NaeI GCCGGC 1 cut(s) 66
NdeII GATC 2 cut(s) 168, 224
NgoMIV GCCGGC 1 cut(s) 64
NlaIII CATG 2 cut(s) 169, 175
NmuCI GTSAC 1 cut(s) 265
PagI TCATGA 1 cut(s) 171
PauI GCGCGC 1 cut(s) 297
PdiI GCCGGC 1 cut(s) 66
PfeI GAWTC 1 cut(s) 92
PkrI GCNGC 1 cut(s) 22
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
Ppu21I YACGTR 1 cut(s) 14
PpuMI RGGWCCY 1 cut(s) 52
Psp5II RGGWCCY 1 cut(s) 52
PspPI GGNCC 1 cut(s) 52
PspPPI RGGWCCY 1 cut(s) 52
PteI GCGCGC 1 cut(s) 297
RseI CAYNNNNRTG 1 cut(s) 170
SaqAI TTAA 1 cut(s) 275
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 2 cut(s) 168, 224
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 1 cut(s) 155
SetI ASST 6 cut(s) 16, 31, 57, 149, 185, 195
SfaNI GCATC 2 cut(s) 77, 126
SfcI CTRYAG 1 cut(s) 56
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 170
Sse9I AATT 3 cut(s) 77, 87, 125
SsiI CCGC 1 cut(s) 262
TaiI ACGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 153
TaqII GACCGA 1 cut(s) 207
TasI AATT 3 cut(s) 77, 87, 125
TfiI GAWTC 1 cut(s) 92
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 188
TspGWI ACGGA 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 52
XapI RAATTY 2 cut(s) 77, 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.