RchiOBHm_Chr5g0024631

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000286
Physical Location & Seq
Reverse (-)
18715519 .. 18716085
567 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30437

Sequence Viewer

Length: 567 bp
ATGGACGAGTTGCATCAGGCAGCTTCAGCATATTACAACAACGGGTCACCTGAGATTCAGCAACTAGCATGGAGCTTCTTCCAATCCATGGACACAAACTGCGACGGCCAAGTTGACTGGAACGAGTTCTCCACATTTCTGTACCAAAGCGGCAACCAATGGATCAGCCCCAGCTTCTTCAACGATCTGGATCGGAACCACGACGGTGCTCTCGATTTCATGGAAGTTCTCACGTTTTACTATATCATCAAGACTAGGAGCTTCTGGTGCAGCATCTGCGGGATTTGTCTCACGGGGCTCTACTTCACTTGTGTTGAGTGCTTTGACAACGTCAACTCAAACACTTACGATCTTTGTCCCAGATGCTATGAATCGAAGCCGGAAAGGCAAAGGTATAACCAGCAGAGTGATCACCATCATGACTACTTTATGGATAACCATATGTTGCTCAGATCAAAGAGAGGAGGATATGGTTCTGCAATCCTGGTATGGTTCTTAAATACACTTGTTGGAATCAATTTGGTCCAAAAACTCCTAGCTTCCATATATATGATCCAGTGTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8 ZZ

Protein Analysis

188

Amino Acids

22.06

Weight (kDa)

5.05

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 22 - 81 4.5e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 88
AciI CCGC 2 cut(s) 150, 279
AclWI GGATC 3 cut(s) 170, 198, 547
AcoI YGGCCR 1 cut(s) 106
AcuI CTGAAG 1 cut(s) 9
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 1 cut(s) 483
AleI CACNNNNGTG 1 cut(s) 204
AloI GAACNNNNNNTCC 2 cut(s) 113, 145
AluBI AGCT 5 cut(s) 23, 75, 174, 261, 539
AluI AGCT 5 cut(s) 23, 75, 174, 261, 539
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 3 cut(s) 170, 198, 547
AlwNI CAGNNNCTG 1 cut(s) 276
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 2 cut(s) 20, 270
Asp700I GAANNNNTTC 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 523
AsuHPI GGTGA 2 cut(s) 39, 404
AvaII GGWCC 1 cut(s) 523
BanII GRGCYC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 2 cut(s) 32, 282
BccI CCATC 1 cut(s) 423
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 1 cut(s) 485
BclI TGATCA 1 cut(s) 409
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 3 cut(s) 65, 255, 536
BglI GCCNNNNNGGC 1 cut(s) 385
BisI GCNGC 3 cut(s) 21, 151, 271
BlsI GCNGC 3 cut(s) 22, 152, 272
Bme1390I CCNGG 1 cut(s) 485
Bme18I GGWCC 1 cut(s) 523
BmgT120I GGNCC 1 cut(s) 523
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 1 cut(s) 485
BmsI GCATC 3 cut(s) 22, 282, 353
BsaBI GATNNNNATC 2 cut(s) 189, 414
BsaJI CCNNGG 1 cut(s) 87
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 2 cut(s) 122, 556
Bse8I GATNNNNATC 2 cut(s) 189, 414
BseBI CCWGG 1 cut(s) 485
BseDI CCNNGG 1 cut(s) 87
BseJI GATNNNNATC 2 cut(s) 189, 414
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMII CTCAG 2 cut(s) 42, 463
BseNI ACTGG 2 cut(s) 122, 556
BseRI GAGGAG 1 cut(s) 477
BseXI GCAGC 2 cut(s) 32, 282
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 289
BshFI GGCC 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 342
BsnI GGCC 1 cut(s) 108
Bsp1286I GDGCHC 2 cut(s) 211, 300
Bsp143I GATC 7 cut(s) 162, 184, 190, 349, 409, 452, 552
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 2 cut(s) 150, 279
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 2 cut(s) 43, 462
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 1 cut(s) 197
BspPI GGATC 3 cut(s) 170, 198, 547
BsrI ACTGG 2 cut(s) 122, 556
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 7 cut(s) 162, 184, 190, 349, 409, 452, 552
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 485
Bst4CI ACNGT 1 cut(s) 206
BstAPI GCANNNNNTGC 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 51, 449
BstDSI CCRYGG 1 cut(s) 87
BstEII GGTNACC 1 cut(s) 45
BstKTI GATC 7 cut(s) 165, 187, 193, 352, 412, 455, 555
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 7 cut(s) 162, 184, 190, 349, 409, 452, 552
BstMWI GCNNNNNNNGC 4 cut(s) 26, 267, 276, 385
BstNI CCWGG 1 cut(s) 485
BstPI GGTNACC 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 483
BstV1I GCAGC 2 cut(s) 32, 282
BsuRI GGCC 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 563
CaiI CAGNNNCTG 1 cut(s) 276
CciI TCATGA 1 cut(s) 418
Cfr13I GGNCC 1 cut(s) 523
Csp6I GTAC 1 cut(s) 142
CviAII CATG 4 cut(s) 69, 88, 220, 419
CviJI RGCY 9 cut(s) 23, 75, 108, 168, 174, 261, 298, 379, 539
CviKI_1 RGCY 9 cut(s) 23, 75, 108, 168, 174, 261, 298, 379, 539
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 2 cut(s) 51, 449
DpnI GATC 7 cut(s) 164, 186, 192, 351, 411, 454, 554
DpnII GATC 7 cut(s) 162, 184, 190, 349, 409, 452, 552
EaeI YGGCCR 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 9
Eco91I GGTNACC 1 cut(s) 45
EcoO65I GGTNACC 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 483
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 4 cut(s) 72, 91, 223, 422
FaqI GGGAC 1 cut(s) 342
FatI CATG 4 cut(s) 68, 87, 219, 418
FauI CCCGC 1 cut(s) 272
FauNDI CATATG 1 cut(s) 441
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 3 cut(s) 21, 151, 271
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 3 cut(s) 21, 151, 271
FspBI CTAG 3 cut(s) 65, 255, 536
GluI GCNGC 3 cut(s) 21, 151, 271
GsaI CCCAGC 1 cut(s) 174
HaeIII GGCC 1 cut(s) 108
HapII CCGG 1 cut(s) 380
Hin1II CATG 4 cut(s) 72, 91, 223, 422
HincII GTYRAC 2 cut(s) 115, 334
HindII GTYRAC 2 cut(s) 115, 334
HinfI GANTC 3 cut(s) 55, 371, 513
HpaII CCGG 1 cut(s) 380
HphI GGTGA 2 cut(s) 39, 404
Hpy166II GTNNAC 2 cut(s) 115, 334
Hpy188I TCNGA 2 cut(s) 195, 452
Hpy188III TCNNGA 4 cut(s) 188, 212, 250, 419
Hpy8I GTNNAC 2 cut(s) 115, 334
Hpy99I CGWCG 2 cut(s) 107, 206
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 2 cut(s) 233, 330
HpyCH4V TGCA 3 cut(s) 13, 270, 479
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 267, 276, 385
HpyF3I CTNAG 2 cut(s) 51, 449
HpySE526I ACGT 2 cut(s) 233, 330
Hsp92II CATG 4 cut(s) 72, 91, 223, 422
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 7 cut(s) 162, 184, 190, 349, 409, 452, 552
LmnI GCTCC 2 cut(s) 72, 258
Lsp1109I GCAGC 2 cut(s) 32, 282
LweI GCATC 3 cut(s) 22, 282, 353
MaeI CTAG 3 cut(s) 65, 255, 536
MaeII ACGT 2 cut(s) 233, 330
MaeIII GTNAC 1 cut(s) 45
MalI GATC 7 cut(s) 164, 186, 192, 351, 411, 454, 554
MboI GATC 7 cut(s) 162, 184, 190, 349, 409, 452, 552
MboII GAAGA 2 cut(s) 70, 169
MhlI GDGCHC 2 cut(s) 211, 300
MluCI AATT 1 cut(s) 517
MmeI TCCRAC 1 cut(s) 490
MnlI CCTC 2 cut(s) 455, 458
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 2 cut(s) 497, 565
MslI CAYNNNNRTG 3 cut(s) 204, 417, 548
MspI CCGG 1 cut(s) 380
MspR9I CCNGG 1 cut(s) 485
MvaI CCWGG 1 cut(s) 485
MwoI GCNNNNNNNGC 4 cut(s) 26, 267, 276, 385
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 441
NdeII GATC 7 cut(s) 162, 184, 190, 349, 409, 452, 552
NlaIII CATG 4 cut(s) 72, 91, 223, 422
NlaIV GGNNCC 1 cut(s) 197
NmuCI GTSAC 1 cut(s) 45
OliI CACNNNNGTG 1 cut(s) 204
PagI TCATGA 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 3 cut(s) 55, 371, 513
PflFI GACNNNGTC 1 cut(s) 329
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 3 cut(s) 22, 152, 272
Psp6I CCWGG 1 cut(s) 483
PspEI GGTNACC 1 cut(s) 45
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 1 cut(s) 483
PspN4I GGNNCC 1 cut(s) 197
PspPI GGNCC 1 cut(s) 523
PstNI CAGNNNCTG 1 cut(s) 276
PsyI GACNNNGTC 1 cut(s) 329
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
RseI CAYNNNNRTG 3 cut(s) 204, 417, 548
SaqAI TTAA 2 cut(s) 497, 565
SatI GCNGC 3 cut(s) 21, 151, 271
Sau3AI GATC 7 cut(s) 162, 184, 190, 349, 409, 452, 552
Sau96I GGNCC 1 cut(s) 523
ScrFI CCNGG 1 cut(s) 485
SduI GDGCHC 2 cut(s) 211, 300
SetI ASST 9 cut(s) 25, 52, 77, 176, 236, 263, 333, 395, 541
SfaNI GCATC 3 cut(s) 22, 282, 353
SinI GGWCC 1 cut(s) 523
SmiMI CAYNNNNRTG 3 cut(s) 204, 417, 548
Sse9I AATT 1 cut(s) 517
SsiI CCGC 2 cut(s) 150, 279
SspMI CTAG 3 cut(s) 65, 255, 536
StyD4I CCNGG 1 cut(s) 483
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 2 cut(s) 236, 333
TaqI TCGA 2 cut(s) 213, 374
TasI AATT 1 cut(s) 517
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 3 cut(s) 55, 371, 513
Tru1I TTAA 2 cut(s) 497, 565
Tru9I TTAA 2 cut(s) 497, 565
TscAI CASTG 1 cut(s) 563
TseFI GTSAC 1 cut(s) 45
TseI GCWGC 2 cut(s) 20, 270
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 2 cut(s) 208, 384
TspRI CASTG 1 cut(s) 563
Tth111I GACNNNGTC 1 cut(s) 329
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 523
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 3 cut(s) 65, 255, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.