Prupe.1G049600_v2.0.a1

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000286
Physical Location & Seq
Forward (+)
3486625 .. 3489087
2463 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G049600.1

Sequence Viewer

Length: 819 bp
ATGGAGGAGTTGCGTCAAATTGCATTTGCATATTACAAGGATGTACCGGAGCACGTGAAGATGTGGGTGCAAGAGTTCTTGTCGGAAATCGACAGGGATGGGAACGAAAAAGTGAGCCTGCAAGAGTTCTTGGCATACATGGGCATGCATGAAGATTCGAAGCATCTCTGTACCCCAAGTTTCTTCGATGAGCTAAAGCAGGAAGGCAGGGAAGAGTTAGAGTTCATAGACTTGCTGGCTCTCTTCTACATTGTTTCCACTGGAAGGCCATTCTGCAGTGGCCACTGCAAAAACTTCATAAAGGGAGGGTATTTCACTTGCGTCCAATGCTTTTATCATTCCCCAAAAACCTTCGATCTTTGCGCCGTTTGTTACCTTGACGGCAAGTATGTCCATCGCCACAAAAACTTCCTTGATGACTTCCTAGTGCTAAAAACCAAGAGGGCAGTGGGAGATAATTTGAACCAGCATGGAGAAGCATCAAGCATAACTAGTAAGGCAAAGGCACCAGAGTCTGGCACTGGCAGTACACCAGCAGAGACAATGAATTCTTCACGTTCTTCTTCCTCTTCTTCATCATCATTATCTTTTTCTTCTTCTACTACTACTGCTACTACAGCAGAGTCAACAAACGACTCTACTTCTTCTGGTACACTCACACCAAGGACAAGCAGTTCCACTACTACATCCAATGCACTGGTTCCCGTGAATCCAAGGCGTAATGATACAAGTGCCGGGAGGAAAGCGTTAAAAGCGATAGAGATTCTGCTCGCAGTGGGAAATATCTTTGTTACTTCCAGTCAGTGTACCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5

Protein Analysis

273

Amino Acids

30.01

Weight (kDa)

6.07

Isoelectric Point (pI)

56.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 505
AccB7I CCANNNNNTGG 1 cut(s) 515
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 1 cut(s) 547
AcvI CACGTG 1 cut(s) 55
AfaI GTAC 5 cut(s) 45, 172, 529, 652, 808
AfiI CCNNNNNNNGG 2 cut(s) 264, 515
AgsI TTSAA 1 cut(s) 463
AhlI ACTAGT 1 cut(s) 491
AloI GAACNNNNNNTCC 2 cut(s) 658, 690
AluBI AGCT 1 cut(s) 193
AluI AGCT 1 cut(s) 193
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 1 cut(s) 533
AlwNI CAGNNNCTG 1 cut(s) 515
AoxI GGCC 2 cut(s) 266, 280
ApoI RAATTY 1 cut(s) 547
AspLEI GCGC 1 cut(s) 365
AsuC2I CCSGG 1 cut(s) 736
AsuII TTCGAA 1 cut(s) 158
BalI TGGCCA 1 cut(s) 282
BanI GGYRCC 1 cut(s) 505
BbrPI CACGTG 1 cut(s) 55
Bbv12I GWGCWC 1 cut(s) 54
BccI CCATC 2 cut(s) 92, 402
BceAI ACGGC 2 cut(s) 350, 397
BcnI CCSGG 1 cut(s) 736
BcoDI GTCTC 1 cut(s) 533
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 2 cut(s) 425, 492
BfmI CTRYAG 2 cut(s) 274, 615
Bme1390I CCNGG 1 cut(s) 736
BmiI GGNNCC 2 cut(s) 507, 702
BmrFI CCNGG 1 cut(s) 736
BmsI GCATC 2 cut(s) 172, 488
Bpu14I TTCGAA 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 736
BsaAI YACGTR 1 cut(s) 55
BsaJI CCNNGG 2 cut(s) 662, 713
BsaWI WCCGGW 1 cut(s) 46
Bsc4I CCNNNNNNNGG 2 cut(s) 264, 515
Bse1I ACTGG 4 cut(s) 265, 526, 702, 798
BseDI CCNNGG 2 cut(s) 662, 713
BseGI GGATG 3 cut(s) 46, 103, 686
BseLI CCNNNNNNNGG 2 cut(s) 264, 515
BseNI ACTGG 4 cut(s) 265, 526, 702, 798
BseRI GAGGAG 1 cut(s) 20
BshFI GGCC 2 cut(s) 268, 282
BshNI GGYRCC 1 cut(s) 505
BsiHKAI GWGCWC 1 cut(s) 54
BsiSI CCGG 2 cut(s) 47, 735
BslI CCNNNNNNNGG 2 cut(s) 264, 515
BsmAI GTCTC 1 cut(s) 533
BsnI GGCC 2 cut(s) 268, 282
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 1 cut(s) 355
BspANI GGCC 2 cut(s) 268, 282
BspLI GGNNCC 2 cut(s) 507, 702
BspMAI CTGCAG 1 cut(s) 278
BspT104I TTCGAA 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 505
BsrI ACTGG 4 cut(s) 265, 526, 702, 798
BssECI CCNNGG 2 cut(s) 662, 713
BssMI GATC 1 cut(s) 355
BssT1I CCWWGG 2 cut(s) 662, 713
Bst6I CTCTTC 3 cut(s) 207, 248, 574
BstBAI YACGTR 1 cut(s) 55
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 4 cut(s) 119, 146, 237, 771
BstF5I GGATG 3 cut(s) 46, 103, 686
BstHHI GCGC 1 cut(s) 365
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 1 cut(s) 533
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 3 cut(s) 327, 617, 752
BstNSI RCATGY 1 cut(s) 148
BstSCI CCNGG 1 cut(s) 734
BstSFI CTRYAG 2 cut(s) 274, 615
BstXI CCANNNNNNTGG 1 cut(s) 697
BsuRI GGCC 2 cut(s) 268, 282
BtgZI GCGATG 1 cut(s) 380
BtsCI GGATG 3 cut(s) 46, 103, 686
BtsI GCAGTG 4 cut(s) 283, 283, 453, 780
BtsIMutI CAGTG 8 cut(s) 258, 283, 283, 453, 519, 695, 780, 809
Cac8I GCNNGC 4 cut(s) 119, 146, 237, 771
CaiI CAGNNNCTG 1 cut(s) 515
CfoI GCGC 1 cut(s) 365
CseI GACGC 2 cut(s) 2, 310
Csp6I GTAC 5 cut(s) 44, 171, 528, 651, 807
CviAII CATG 4 cut(s) 139, 145, 149, 470
CviJI RGCY 5 cut(s) 117, 193, 239, 268, 282
CviKI_1 RGCY 5 cut(s) 117, 193, 239, 268, 282
CviQI GTAC 5 cut(s) 44, 171, 528, 651, 807
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
EaeI YGGCCR 1 cut(s) 280
Eam1104I CTCTTC 3 cut(s) 207, 248, 574
EarI CTCTTC 3 cut(s) 207, 248, 574
Eco130I CCWWGG 2 cut(s) 662, 713
Eco72I CACGTG 1 cut(s) 55
EcoRI GAATTC 1 cut(s) 547
EcoT14I CCWWGG 2 cut(s) 662, 713
EcoT22I ATGCAT 1 cut(s) 150
ErhI CCWWGG 2 cut(s) 662, 713
FaeI CATG 4 cut(s) 142, 148, 152, 473
FatI CATG 4 cut(s) 138, 144, 148, 469
FokI GGATG 3 cut(s) 53, 110, 673
FspBI CTAG 2 cut(s) 425, 492
GlaI GCGC 1 cut(s) 364
HaeIII GGCC 2 cut(s) 268, 282
HapII CCGG 2 cut(s) 47, 735
HgaI GACGC 2 cut(s) 2, 310
HhaI GCGC 1 cut(s) 365
Hin1II CATG 4 cut(s) 142, 148, 152, 473
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HincII GTYRAC 1 cut(s) 627
HindII GTYRAC 1 cut(s) 627
HinfI GANTC 6 cut(s) 155, 512, 623, 635, 709, 763
HpaII CCGG 2 cut(s) 47, 735
Hpy166II GTNNAC 4 cut(s) 530, 627, 653, 807
Hpy188I TCNGA 1 cut(s) 85
Hpy8I GTNNAC 4 cut(s) 530, 627, 653, 807
HpyAV CCTTC 3 cut(s) 197, 258, 361
HpyCH4IV ACGT 2 cut(s) 54, 556
HpyCH4V TGCA 8 cut(s) 23, 29, 70, 121, 148, 276, 288, 695
HpyF10VI GCNNNNNNNGC 3 cut(s) 327, 617, 752
HpySE526I ACGT 2 cut(s) 54, 556
Hsp92II CATG 4 cut(s) 142, 148, 152, 473
HspAI GCGC 1 cut(s) 363
Kzo9I GATC 1 cut(s) 355
LmnI GCTCC 1 cut(s) 49
LweI GCATC 2 cut(s) 172, 488
MaeI CTAG 2 cut(s) 425, 492
MaeII ACGT 2 cut(s) 54, 556
MaeIII GTNAC 2 cut(s) 371, 790
MalI GATC 1 cut(s) 357
MboI GATC 1 cut(s) 355
MhlI GDGCHC 1 cut(s) 54
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 3 cut(s) 18, 457, 547
MluNI TGGCCA 1 cut(s) 282
MlyI GAGTC 3 cut(s) 521, 629, 632
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 4 cut(s) 299, 435, 577, 732
Mox20I TGGCCA 1 cut(s) 282
Mph1103I ATGCAT 1 cut(s) 150
MscI TGGCCA 1 cut(s) 282
MseI TTAA 1 cut(s) 749
MslI CAYNNNNRTG 1 cut(s) 143
Msp20I TGGCCA 1 cut(s) 282
MspI CCGG 2 cut(s) 47, 735
MspR9I CCNGG 1 cut(s) 736
MwoI GCNNNNNNNGC 3 cut(s) 327, 617, 752
NciI CCSGG 1 cut(s) 736
NdeII GATC 1 cut(s) 355
NlaIII CATG 4 cut(s) 142, 148, 152, 473
NlaIV GGNNCC 2 cut(s) 507, 702
NsiI ATGCAT 1 cut(s) 150
NspI RCATGY 1 cut(s) 148
NspV TTCGAA 1 cut(s) 158
PaeI GCATGC 1 cut(s) 148
PfeI GAWTC 3 cut(s) 155, 709, 763
PflMI CCANNNNNTGG 1 cut(s) 515
PleI GAGTC 3 cut(s) 520, 629, 631
PmaCI CACGTG 1 cut(s) 55
PmlI CACGTG 1 cut(s) 55
PpsI GAGTC 3 cut(s) 520, 629, 631
Ppu21I YACGTR 1 cut(s) 55
PspCI CACGTG 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 507, 702
PstI CTGCAG 1 cut(s) 278
PstNI CAGNNNCTG 1 cut(s) 515
RsaI GTAC 5 cut(s) 45, 172, 529, 652, 808
RsaNI GTAC 5 cut(s) 44, 171, 528, 651, 807
RseI CAYNNNNRTG 1 cut(s) 143
SaqAI TTAA 1 cut(s) 749
Sau3AI GATC 1 cut(s) 355
SchI GAGTC 3 cut(s) 521, 629, 632
ScrFI CCNGG 1 cut(s) 736
SduI GDGCHC 1 cut(s) 54
SetI ASST 5 cut(s) 57, 195, 353, 378, 559
SfaNI GCATC 2 cut(s) 172, 488
SfcI CTRYAG 2 cut(s) 274, 615
SfuI TTCGAA 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 143
SpeI ACTAGT 1 cut(s) 491
SphI GCATGC 1 cut(s) 148
Sse9I AATT 3 cut(s) 18, 457, 547
SspMI CTAG 2 cut(s) 425, 492
StyD4I CCNGG 1 cut(s) 734
StyI CCWWGG 2 cut(s) 662, 713
TaiI ACGT 2 cut(s) 57, 559
TaqI TCGA 4 cut(s) 90, 158, 186, 354
TasI AATT 3 cut(s) 18, 457, 547
TatI WGTACW 1 cut(s) 527
TfiI GAWTC 3 cut(s) 155, 709, 763
Tru1I TTAA 1 cut(s) 749
Tru9I TTAA 1 cut(s) 749
TscAI CASTG 8 cut(s) 265, 283, 290, 453, 526, 702, 780, 809
TspDTI ATGAA 5 cut(s) 165, 214, 286, 560, 564
TspRI CASTG 8 cut(s) 265, 283, 290, 453, 526, 702, 780, 809
Van91I CCANNNNNTGG 1 cut(s) 515
XapI RAATTY 1 cut(s) 547
XceI RCATGY 1 cut(s) 148
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XspI CTAG 2 cut(s) 425, 492
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.