Rroxscaffold_1G00054640

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000286
Physical Location & Seq
Forward (+)
76025936 .. 76026985
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00054640.1

Sequence Viewer

Length: 639 bp
ATGGACGAGCTGCGTCAAGCAGCTTCTGCATATTACAACAACGGGTCACCTGAGATTCAGCAACTAGCATGGAGCTTCTTCCAATCCATGGACACAAACTGCGACAGCCAAGTTGACTGGAACGAGTTCTCCGCATTTCTCTACCAAAGCGGCAACCAATGGATCAGCCCCAGCTTCTTCAACGATCTTGACCGGAACCACGACGGTGCCCTCGATTTCATGGAAGTTCTCACGTTTTACTACATCATCAAGACCAGGAGCTTCTGGTGCAGTGTTTGCGGGATATGTCTCACGGGGCTCTACTTCACTTGTGTTGAGTGCTTTGACAACGTGAACTCGAACACTTATGATCTTTGTCCCGGATGCTATGAGTCGAAGCCGGAAAGGCAAAGGTATAACCAGCAGAGTGATCACCATCATGCCTCCTTTATGGATAACCATATGTTGCTCAGATCAAAGAGAGGAGGATATGGTTCTGCAAACCTGGCTACTTTGCATAGTTTCAGTAATATTGACAAAATGCATGATATGCAGGCCATAGATTCACCTGGAGGTGGACCTGGACGGACCAGAATGCGCCAAACTATGAAAGCATTGGATACGGCAGTGAATGTGGCAACTTTATGTAGCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8 ZZ

Protein Analysis

212

Amino Acids

24.19

Weight (kDa)

5.22

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 22 - 81 3.6e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 206
AccB7I CCANNNNNTGG 1 cut(s) 88
AciI CCGC 3 cut(s) 132, 150, 279
AclWI GGATC 1 cut(s) 170
AfiI CCNNNNNNNGG 2 cut(s) 88, 554
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 4 cut(s) 254, 483, 547, 559
AleI CACNNNNGTG 1 cut(s) 204
AloI GAACNNNNNNTCC 2 cut(s) 113, 145
AluBI AGCT 5 cut(s) 10, 23, 75, 174, 261
AluI AGCT 5 cut(s) 10, 23, 75, 174, 261
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 1 cut(s) 170
AlwNI CAGNNNCTG 1 cut(s) 26
AoxI GGCC 1 cut(s) 534
ApeKI GCWGC 2 cut(s) 10, 20
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 579
AspS9I GGNCC 2 cut(s) 557, 567
AsuC2I CCSGG 1 cut(s) 360
AsuHPI GGTGA 3 cut(s) 39, 404, 537
AvaII GGWCC 2 cut(s) 557, 567
BaeGI GKGCMC 1 cut(s) 211
BaeI ACNNNNGTAYC 2 cut(s) 591, 624
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 1 cut(s) 300
BbvI GCAGC 1 cut(s) 32
BccI CCATC 1 cut(s) 423
BceAI ACGGC 1 cut(s) 618
BciT130I CCWGG 4 cut(s) 256, 485, 549, 561
BciVI GTATCC 1 cut(s) 592
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 1 cut(s) 360
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 1 cut(s) 65
BfuI GTATCC 1 cut(s) 592
BglI GCCNNNNNGGC 1 cut(s) 385
BisI GCNGC 3 cut(s) 11, 21, 151
BlsI GCNGC 3 cut(s) 12, 22, 152
Bme1390I CCNGG 5 cut(s) 256, 360, 485, 549, 561
Bme18I GGWCC 2 cut(s) 557, 567
BmgT120I GGNCC 2 cut(s) 557, 567
BmiI GGNNCC 2 cut(s) 197, 208
BmrFI CCNGG 5 cut(s) 256, 360, 485, 549, 561
BmsI GCATC 1 cut(s) 353
BpmI CTGGAG 1 cut(s) 570
BpuMI CCSGG 1 cut(s) 360
BsaBI GATNNNNATC 1 cut(s) 414
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 554
Bse1I ACTGG 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 4 cut(s) 256, 485, 549, 561
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 368
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 88, 554
BseMII CTCAG 2 cut(s) 42, 463
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 477
BseSI GKGCMC 1 cut(s) 211
BseXI GCAGC 1 cut(s) 32
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 289
BshFI GGCC 1 cut(s) 536
BshNI GGYRCC 1 cut(s) 206
BsiSI CCGG 3 cut(s) 193, 360, 380
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 2 cut(s) 88, 554
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 342
BsmI GAATGC 1 cut(s) 579
BsnI GGCC 1 cut(s) 536
Bsp1286I GDGCHC 2 cut(s) 211, 300
Bsp143I GATC 5 cut(s) 162, 184, 349, 409, 452
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 3 cut(s) 132, 150, 279
BspANI GGCC 1 cut(s) 536
BspCNI CTCAG 2 cut(s) 43, 462
BspLI GGNNCC 2 cut(s) 197, 208
BspPI GGATC 1 cut(s) 170
BspT107I GGYRCC 1 cut(s) 206
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 5 cut(s) 162, 184, 349, 409, 452
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 4 cut(s) 256, 485, 549, 561
Bst4CI ACNGT 1 cut(s) 206
BstAPI GCANNNNNTGC 3 cut(s) 26, 276, 529
BstC8I GCNNGC 1 cut(s) 534
BstDEI CTNAG 2 cut(s) 51, 449
BstDSI CCRYGG 1 cut(s) 87
BstEII GGTNACC 1 cut(s) 45
BstF5I GGATG 1 cut(s) 368
BstHHI GCGC 1 cut(s) 579
BstKTI GATC 5 cut(s) 165, 187, 352, 412, 455
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 5 cut(s) 162, 184, 349, 409, 452
BstMWI GCNNNNNNNGC 6 cut(s) 26, 267, 276, 385, 485, 529
BstNI CCWGG 4 cut(s) 256, 485, 549, 561
BstPI GGTNACC 1 cut(s) 45
BstSCI CCNGG 5 cut(s) 254, 358, 483, 547, 559
BstSLI GKGCMC 1 cut(s) 211
BstV1I GCAGC 1 cut(s) 32
BsuI GTATCC 1 cut(s) 592
BsuRI GGCC 1 cut(s) 536
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 1 cut(s) 368
BtsI GCAGTG 2 cut(s) 277, 612
BtsIMutI CAGTG 2 cut(s) 277, 612
Cac8I GCNNGC 1 cut(s) 534
CaiI CAGNNNCTG 1 cut(s) 26
CfoI GCGC 1 cut(s) 579
Cfr13I GGNCC 2 cut(s) 557, 567
CseI GACGC 1 cut(s) 2
CviAII CATG 5 cut(s) 69, 88, 220, 419, 524
DdeI CTNAG 2 cut(s) 51, 449
DpnI GATC 5 cut(s) 164, 186, 351, 411, 454
DpnII GATC 5 cut(s) 162, 184, 349, 409, 452
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 557, 567
Eco91I GGTNACC 1 cut(s) 45
EcoO65I GGTNACC 1 cut(s) 45
EcoRII CCWGG 4 cut(s) 254, 483, 547, 559
EcoT14I CCWWGG 1 cut(s) 87
EcoT22I ATGCAT 1 cut(s) 525
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 5 cut(s) 72, 91, 223, 422, 527
FaqI GGGAC 1 cut(s) 342
FatI CATG 5 cut(s) 68, 87, 219, 418, 523
FauI CCCGC 1 cut(s) 272
FauNDI CATATG 1 cut(s) 441
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 3 cut(s) 11, 21, 151
FokI GGATG 1 cut(s) 375
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 3 cut(s) 11, 21, 151
FspBI CTAG 1 cut(s) 65
GlaI GCGC 1 cut(s) 578
GluI GCNGC 3 cut(s) 11, 21, 151
GsaI CCCAGC 1 cut(s) 174
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 1 cut(s) 536
HapII CCGG 3 cut(s) 193, 360, 380
HgaI GACGC 1 cut(s) 2
HhaI GCGC 1 cut(s) 579
Hin1II CATG 5 cut(s) 72, 91, 223, 422, 527
Hin6I GCGC 1 cut(s) 577
HinP1I GCGC 1 cut(s) 577
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 3 cut(s) 55, 371, 542
HpaII CCGG 3 cut(s) 193, 360, 380
HphI GGTGA 3 cut(s) 39, 404, 537
Hpy166II GTNNAC 3 cut(s) 115, 334, 557
Hpy188I TCNGA 1 cut(s) 452
Hpy188III TCNNGA 2 cut(s) 188, 250
Hpy8I GTNNAC 3 cut(s) 115, 334, 557
Hpy99I CGWCG 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 2 cut(s) 233, 330
HpyCH4V TGCA 6 cut(s) 29, 270, 479, 496, 523, 532
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 267, 276, 385, 485, 529
HpyF3I CTNAG 2 cut(s) 51, 449
HpySE526I ACGT 2 cut(s) 233, 330
Hsp92II CATG 5 cut(s) 72, 91, 223, 422, 527
HspAI GCGC 1 cut(s) 577
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 5 cut(s) 162, 184, 349, 409, 452
LmnI GCTCC 2 cut(s) 72, 258
Lsp1109I GCAGC 1 cut(s) 32
LweI GCATC 1 cut(s) 353
MaeI CTAG 1 cut(s) 65
MaeII ACGT 2 cut(s) 233, 330
MaeIII GTNAC 1 cut(s) 45
MalI GATC 5 cut(s) 164, 186, 351, 411, 454
MboI GATC 5 cut(s) 162, 184, 349, 409, 452
MboII GAAGA 2 cut(s) 70, 169
MhlI GDGCHC 2 cut(s) 211, 300
MlyI GAGTC 1 cut(s) 380
MnlI CCTC 5 cut(s) 221, 433, 455, 458, 545
Mph1103I ATGCAT 1 cut(s) 525
MroXI GAANNNNTTC 1 cut(s) 125
MslI CAYNNNNRTG 2 cut(s) 204, 417
MspI CCGG 3 cut(s) 193, 360, 380
MspR9I CCNGG 5 cut(s) 256, 360, 485, 549, 561
Mva1269I GAATGC 1 cut(s) 579
MvaI CCWGG 4 cut(s) 256, 485, 549, 561
MwoI GCNNNNNNNGC 6 cut(s) 26, 267, 276, 385, 485, 529
NciI CCSGG 1 cut(s) 360
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 441
NdeII GATC 5 cut(s) 162, 184, 349, 409, 452
NlaIII CATG 5 cut(s) 72, 91, 223, 422, 527
NlaIV GGNNCC 2 cut(s) 197, 208
NmuCI GTSAC 1 cut(s) 45
NsiI ATGCAT 1 cut(s) 525
OliI CACNNNNGTG 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 579
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 55, 542
PflMI CCANNNNNTGG 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 358
PkrI GCNGC 3 cut(s) 12, 22, 152
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp6I CCWGG 4 cut(s) 254, 483, 547, 559
PspEI GGTNACC 1 cut(s) 45
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 4 cut(s) 254, 483, 547, 559
PspN4I GGNNCC 2 cut(s) 197, 208
PspPI GGNCC 2 cut(s) 557, 567
PstNI CAGNNNCTG 1 cut(s) 26
RseI CAYNNNNRTG 2 cut(s) 204, 417
SatI GCNGC 3 cut(s) 11, 21, 151
Sau3AI GATC 5 cut(s) 162, 184, 349, 409, 452
Sau96I GGNCC 2 cut(s) 557, 567
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 5 cut(s) 256, 360, 485, 549, 561
SduI GDGCHC 2 cut(s) 211, 300
SfaNI GCATC 1 cut(s) 353
SinI GGWCC 2 cut(s) 557, 567
SmiMI CAYNNNNRTG 2 cut(s) 204, 417
SsiI CCGC 3 cut(s) 132, 150, 279
SspI AATATT 1 cut(s) 511
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 5 cut(s) 254, 358, 483, 547, 559
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 2 cut(s) 236, 333
TaqI TCGA 3 cut(s) 213, 338, 374
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 55, 542
TscAI CASTG 2 cut(s) 277, 612
TseFI GTSAC 1 cut(s) 45
TseI GCWGC 2 cut(s) 10, 20
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 2 cut(s) 208, 602
TspGWI ACGGA 1 cut(s) 580
TspRI CASTG 2 cut(s) 277, 612
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 557, 567
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 1 cut(s) 65
Zsp2I ATGCAT 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.