RchiOBHm_Chr4g0395741

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000286
Physical Location & Seq
Forward (+)
11654286 .. 11654699
414 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36816

Sequence Viewer

Length: 414 bp
ATGGCAATGGAAGAACTACATGCAGCTGCTTCAGCATACTACCAGAATGGCACTCAACAGACTCGGAATCTGGCATGGTCTTTCTTCCAATCTATGGACACCAACGGTGATGGTCGAATCAGCTGCTCGGAGTTCAAGGACTTCCTCCGGCAAAGTGGCTACAACTGGATTATTAACGACCCAAACTTCTTCAACAGACTTGACCGCAATGGGGATGGTGGATTGGATTTTGGGGAAGTTCTCACTTTCTACTACATCATTAAAACGAGGAAAATCCGATGCCAAGGGTCCCAGTGCGGCGCATACCTCTTCGGTCTGTATTTCACTTGCGTTGCTTGCTTTGACGGAGCTCATGGGCATACCTTTGATCTCTGTCCTGCCTGCTATCGCAGTCGGAGTTACTATCACCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

137

Amino Acids

15.78

Weight (kDa)

6.49

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 26 - 52 2.8e-06 EF-hand domain
EF-hand_7 PF13499 27 - 85 1.7e-07 EF-hand domain pair
EF-hand_1 PF00036 28 - 50 4.7e-06 EF hand domain
EF-hand_5 PF13202 28 - 49 5.7e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 94
AciI CCGC 2 cut(s) 205, 297
AcuI CTGAAG 1 cut(s) 15
AfiI CCNNNNNNNGG 2 cut(s) 94, 211
AgsI TTSAA 2 cut(s) 136, 193
AluBI AGCT 3 cut(s) 26, 123, 350
AluI AGCT 3 cut(s) 26, 123, 350
Alw21I GWGCWC 1 cut(s) 352
ApeKI GCWGC 3 cut(s) 23, 26, 123
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 1 cut(s) 288
AsuHPI GGTGA 2 cut(s) 119, 398
AvaII GGWCC 1 cut(s) 288
BanII GRGCYC 1 cut(s) 352
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 3 cut(s) 13, 35, 110
BccI CCATC 2 cut(s) 104, 209
BcgI CGANNNNNNTGC 2 cut(s) 105, 139
BfaI CTAG 1 cut(s) 412
BisI GCNGC 4 cut(s) 24, 27, 124, 298
BlsI GCNGC 4 cut(s) 25, 28, 125, 299
Bme18I GGWCC 1 cut(s) 288
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 2 cut(s) 289, 290
BmrI ACTGGG 1 cut(s) 286
BmsI GCATC 1 cut(s) 269
BmuI ACTGGG 1 cut(s) 286
BsaJI CCNNGG 1 cut(s) 283
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 211
Bse1I ACTGG 2 cut(s) 170, 292
Bse3DI GCAATG 2 cut(s) 12, 214
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 1 cut(s) 220
BseLI CCNNNNNNNGG 2 cut(s) 94, 211
BseMI GCAATG 2 cut(s) 12, 214
BseNI ACTGG 2 cut(s) 170, 292
BseXI GCAGC 3 cut(s) 13, 35, 110
BsiHKAI GWGCWC 1 cut(s) 352
BsiSI CCGG 1 cut(s) 148
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 2 cut(s) 94, 211
BsmFI GGGAC 1 cut(s) 274
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 1 cut(s) 367
BspACI CCGC 2 cut(s) 205, 297
BspLI GGNNCC 2 cut(s) 289, 290
BsrDI GCAATG 2 cut(s) 12, 214
BsrI ACTGG 2 cut(s) 170, 292
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 1 cut(s) 367
BssT1I CCWWGG 1 cut(s) 283
Bst4CI ACNGT 1 cut(s) 107
Bst6I CTCTTC 1 cut(s) 314
BstC8I GCNNGC 2 cut(s) 337, 382
BstF5I GGATG 1 cut(s) 220
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 1 cut(s) 370
BstMBI GATC 1 cut(s) 367
BstMWI GCNNNNNNNGC 2 cut(s) 32, 336
BstNSI RCATGY 1 cut(s) 23
BstV1I GCAGC 3 cut(s) 13, 35, 110
BtsCI GGATG 1 cut(s) 220
BtsIMutI CAGTG 1 cut(s) 299
Cac8I GCNNGC 2 cut(s) 337, 382
CfoI GCGC 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 288
CviAII CATG 3 cut(s) 20, 75, 353
CviJI RGCY 4 cut(s) 26, 123, 159, 350
CviKI_1 RGCY 4 cut(s) 26, 123, 159, 350
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 314
EarI CTCTTC 1 cut(s) 314
Ecl136II GAGCTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 15
EcoICRI GAGCTC 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 3 cut(s) 23, 78, 356
FaiI YATR 7 cut(s) 21, 37, 76, 95, 304, 354, 360
FaqI GGGAC 1 cut(s) 274
FatI CATG 3 cut(s) 19, 74, 352
Fnu4HI GCNGC 4 cut(s) 24, 27, 124, 298
FokI GGATG 1 cut(s) 227
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 4 cut(s) 24, 27, 124, 298
FspBI CTAG 1 cut(s) 412
GlaI GCGC 1 cut(s) 301
GluI GCNGC 4 cut(s) 24, 27, 124, 298
HapII CCGG 1 cut(s) 148
HhaI GCGC 1 cut(s) 302
Hin1II CATG 3 cut(s) 23, 78, 356
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HinfI GANTC 3 cut(s) 61, 67, 117
HpaII CCGG 1 cut(s) 148
HphI GGTGA 2 cut(s) 119, 398
Hpy188I TCNGA 4 cut(s) 66, 130, 278, 396
HpyCH4III ACNGT 1 cut(s) 107
HpyCH4V TGCA 1 cut(s) 23
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 336
Hsp92II CATG 3 cut(s) 23, 78, 356
HspAI GCGC 1 cut(s) 300
KflI GGGWCCC 1 cut(s) 288
Kzo9I GATC 1 cut(s) 367
LmnI GCTCC 1 cut(s) 347
LpnPI CCDG 7 cut(s) 56, 56, 151, 161, 305, 390, 394
Lsp1109I GCAGC 3 cut(s) 13, 35, 110
LweI GCATC 1 cut(s) 269
MaeI CTAG 1 cut(s) 412
MaeIII GTNAC 1 cut(s) 398
MalI GATC 1 cut(s) 369
MboI GATC 1 cut(s) 367
MboII GAAGA 4 cut(s) 23, 76, 181, 301
MhlI GDGCHC 1 cut(s) 352
MlyI GAGTC 1 cut(s) 55
MmeI TCCRAC 1 cut(s) 374
MnlI CCTC 3 cut(s) 155, 261, 317
MseI TTAA 2 cut(s) 174, 261
MspA1I CMGCKG 2 cut(s) 26, 123
MspI CCGG 1 cut(s) 148
MwoI GCNNNNNNNGC 2 cut(s) 32, 336
NdeII GATC 1 cut(s) 367
NlaIII CATG 3 cut(s) 23, 78, 356
NlaIV GGNNCC 2 cut(s) 289, 290
NspI RCATGY 1 cut(s) 23
PfeI GAWTC 2 cut(s) 67, 117
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 4 cut(s) 25, 28, 125, 299
PleI GAGTC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 55
PpuMI RGGWCCY 1 cut(s) 288
Psp124BI GAGCTC 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 288
PspN4I GGNNCC 2 cut(s) 289, 290
PspPI GGNCC 1 cut(s) 288
PspPPI RGGWCCY 1 cut(s) 288
PvuII CAGCTG 2 cut(s) 26, 123
SacI GAGCTC 1 cut(s) 352
SaqAI TTAA 2 cut(s) 174, 261
SatI GCNGC 4 cut(s) 24, 27, 124, 298
Sau3AI GATC 1 cut(s) 367
Sau96I GGNCC 1 cut(s) 288
SchI GAGTC 1 cut(s) 55
SduI GDGCHC 1 cut(s) 352
SetI ASST 5 cut(s) 28, 125, 309, 352, 365
SfaNI GCATC 1 cut(s) 269
SinI GGWCC 1 cut(s) 288
SsiI CCGC 2 cut(s) 205, 297
SspMI CTAG 1 cut(s) 412
SstI GAGCTC 1 cut(s) 352
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 1 cut(s) 107
TaqI TCGA 1 cut(s) 115
TaqII GACCGA 1 cut(s) 302
TauI GCSGC 1 cut(s) 300
TfiI GAWTC 2 cut(s) 67, 117
Tru1I TTAA 2 cut(s) 174, 261
Tru9I TTAA 2 cut(s) 174, 261
TscAI CASTG 1 cut(s) 299
TseI GCWGC 3 cut(s) 23, 26, 123
TspGWI ACGGA 1 cut(s) 360
TspRI CASTG 1 cut(s) 299
Van91I CCANNNNNTGG 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 288
XceI RCATGY 1 cut(s) 23
XspI CTAG 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.