RchiOBHm_Chr4g0395461

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000286
Physical Location & Seq
Reverse (-)
11163413 .. 11163943
531 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36790

Sequence Viewer

Length: 531 bp
ATGGGAGACATCAAAGAAATTCGTGATGCTGCTTTAGCTTATTACCATAATTTGTCTGAAGAGCAAAAGCAGAAGGTGTATACCTTTTTTCAAGAAATTGACATAGATGGTGATGGAACAATAACGATGGAGGAGTTCAAGCAGAGGTTTGAAGGTAGTTTCGGCGACAATGTCAACCGTACCTTCGAGGACCTAGACAAAAATGGAGATGGCAGTTTGGATTTTGAGGAATCTATCACTCTTTACTACTCTTTTAAGACCCGGAGGACTATTAGTTGCGATGGATGCAAAGCCTTTTTTCTAAAGGGACTGTATTTCACTTGCGTGGACTGCTTCGAGCATAACCGAACTACAACTTTTGATCTTTGCACCTCCTGCTACCGCAGCCGCAACTACGTTCACGAACACTGCAACTTTTTGGACAACTATGCCTTACTACGTACTAAGGCTGCTTATACCAAATCTGGGTCGACAAACCAGGTGATTTATCAATTGAACTCTTACAGATTAGAAGCCTACTGTCATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

176

Amino Acids

20.61

Weight (kDa)

5.23

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 14 - 50 3.9e-06 EF-hand domain pair
EF-hand_8 PF13833 23 - 51 8e-06 EF-hand domain pair
EF-hand_1 PF00036 28 - 49 6.8e-06 EF hand domain
EF-hand_5 PF13202 29 - 48 5.9e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 80, 470
AciI CCGC 2 cut(s) 382, 388
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 2 cut(s) 181, 442
AfiI CCNNNNNNNGG 1 cut(s) 465
AgsI TTSAA 4 cut(s) 92, 139, 152, 496
AjnI CCWGG 1 cut(s) 477
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 1 cut(s) 38
AluI AGCT 1 cut(s) 38
ApeKI GCWGC 3 cut(s) 29, 384, 449
ApoI RAATTY 1 cut(s) 18
AspS9I GGNCC 1 cut(s) 190
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 2 cut(s) 122, 493
AvaII GGWCC 1 cut(s) 190
BbvI GCAGC 3 cut(s) 16, 396, 436
BccI CCATC 5 cut(s) 101, 107, 121, 203, 275
BciT130I CCWGG 1 cut(s) 479
BcnI CCSGG 1 cut(s) 262
BfaI CTAG 1 cut(s) 194
BisI GCNGC 4 cut(s) 30, 385, 388, 450
BlsI GCNGC 4 cut(s) 31, 386, 389, 451
Bme1390I CCNGG 2 cut(s) 262, 479
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 190
BmrFI CCNGG 2 cut(s) 262, 479
BmsI GCATC 2 cut(s) 16, 275
BpuMI CCSGG 1 cut(s) 262
BsaAI YACGTR 1 cut(s) 440
Bsc4I CCNNNNNNNGG 1 cut(s) 465
BseBI CCWGG 1 cut(s) 479
BseGI GGATG 1 cut(s) 290
BseLI CCNNNNNNNGG 1 cut(s) 465
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 3 cut(s) 16, 396, 436
BsiSI CCGG 1 cut(s) 262
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 465
BsmFI GGGAC 1 cut(s) 321
Bsp143I GATC 1 cut(s) 361
BspACI CCGC 2 cut(s) 382, 388
BspQI GCTCTTC 1 cut(s) 54
BssMI GATC 1 cut(s) 361
BssNAI GTATAC 1 cut(s) 81
Bst1107I GTATAC 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 3 cut(s) 179, 312, 521
Bst6I CTCTTC 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 375
BstBAI YACGTR 1 cut(s) 440
BstDEI CTNAG 1 cut(s) 444
BstF5I GGATG 1 cut(s) 290
BstKTI GATC 1 cut(s) 364
BstMBI GATC 1 cut(s) 361
BstMWI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 375, 384
BstNI CCWGG 1 cut(s) 479
BstSCI CCNGG 2 cut(s) 260, 477
BstSNI TACGTA 1 cut(s) 440
BstV1I GCAGC 3 cut(s) 16, 396, 436
BstZ17I GTATAC 1 cut(s) 81
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 290
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 406
Cfr13I GGNCC 1 cut(s) 190
CsiI ACCWGGT 1 cut(s) 477
Csp6I GTAC 2 cut(s) 180, 441
CviJI RGCY 5 cut(s) 38, 293, 387, 449, 515
CviKI_1 RGCY 5 cut(s) 38, 293, 387, 449, 515
CviQI GTAC 2 cut(s) 180, 441
DdeI CTNAG 1 cut(s) 444
DpnI GATC 1 cut(s) 363
DpnII GATC 1 cut(s) 361
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
Eco105I TACGTA 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 78
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 477
FaiI YATR 7 cut(s) 48, 81, 104, 342, 429, 456, 525
FaqI GGGAC 1 cut(s) 321
FblI GTMKAC 2 cut(s) 80, 470
Fnu4HI GCNGC 4 cut(s) 30, 385, 388, 450
FokI GGATG 1 cut(s) 297
Fsp4HI GCNGC 4 cut(s) 30, 385, 388, 450
FspBI CTAG 1 cut(s) 194
GluI GCNGC 4 cut(s) 30, 385, 388, 450
HapII CCGG 1 cut(s) 262
HincII GTYRAC 2 cut(s) 175, 471
HindII GTYRAC 2 cut(s) 175, 471
HinfI GANTC 1 cut(s) 230
HpaII CCGG 1 cut(s) 262
HphI GGTGA 2 cut(s) 122, 493
Hpy166II GTNNAC 5 cut(s) 81, 175, 328, 400, 471
Hpy188I TCNGA 1 cut(s) 58
Hpy188III TCNNGA 3 cut(s) 23, 92, 401
Hpy8I GTNNAC 5 cut(s) 81, 175, 328, 400, 471
HpyAV CCTTC 3 cut(s) 67, 146, 193
HpyCH4III ACNGT 3 cut(s) 179, 312, 521
HpyCH4IV ACGT 2 cut(s) 396, 439
HpyCH4V TGCA 3 cut(s) 288, 369, 411
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 375, 384
HpyF3I CTNAG 1 cut(s) 444
HpySE526I ACGT 2 cut(s) 396, 439
Kzo9I GATC 1 cut(s) 361
LguI GCTCTTC 1 cut(s) 54
LpnPI CCDG 5 cut(s) 275, 388, 450, 464, 491
Lsp1109I GCAGC 3 cut(s) 16, 396, 436
LweI GCATC 2 cut(s) 16, 275
MabI ACCWGGT 1 cut(s) 477
MaeI CTAG 1 cut(s) 194
MaeII ACGT 2 cut(s) 396, 439
MalI GATC 1 cut(s) 363
MboI GATC 1 cut(s) 361
MboII GAAGA 1 cut(s) 71
MfeI CAATTG 1 cut(s) 491
MluCI AATT 4 cut(s) 18, 49, 96, 491
MnlI CCTC 6 cut(s) 124, 138, 181, 220, 258, 382
MseI TTAA 1 cut(s) 255
MslI CAYNNNNRTG 1 cut(s) 323
MspI CCGG 1 cut(s) 262
MspR9I CCNGG 2 cut(s) 262, 479
MunI CAATTG 1 cut(s) 491
MvaI CCWGG 1 cut(s) 479
MwoI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 375, 384
NciI CCSGG 1 cut(s) 262
NdeII GATC 1 cut(s) 361
OliI CACNNNNGTG 1 cut(s) 323
PciSI GCTCTTC 1 cut(s) 54
PfeI GAWTC 1 cut(s) 230
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 4 cut(s) 31, 386, 389, 451
Ppu21I YACGTR 1 cut(s) 440
PpuMI RGGWCCY 1 cut(s) 190
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspPI GGNCC 1 cut(s) 190
PspPPI RGGWCCY 1 cut(s) 190
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 2 cut(s) 181, 442
RsaNI GTAC 2 cut(s) 180, 441
RseI CAYNNNNRTG 1 cut(s) 323
SalI GTCGAC 1 cut(s) 469
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 4 cut(s) 30, 385, 388, 450
Sau3AI GATC 1 cut(s) 361
Sau96I GGNCC 1 cut(s) 190
ScrFI CCNGG 2 cut(s) 262, 479
SexAI ACCWGGT 1 cut(s) 477
SfaNI GCATC 2 cut(s) 16, 275
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 323
SnaBI TACGTA 1 cut(s) 440
Sse9I AATT 4 cut(s) 18, 49, 96, 491
SsiI CCGC 2 cut(s) 382, 388
SspMI CTAG 1 cut(s) 194
StyD4I CCNGG 2 cut(s) 260, 477
TaaI ACNGT 3 cut(s) 179, 312, 521
TaiI ACGT 2 cut(s) 399, 442
TaqI TCGA 3 cut(s) 186, 336, 470
TasI AATT 4 cut(s) 18, 49, 96, 491
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 1 cut(s) 413
TseI GCWGC 3 cut(s) 29, 384, 449
TspRI CASTG 1 cut(s) 413
Tth111I GACNNNGTC 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 190
XapI RAATTY 1 cut(s) 18
XmiI GTMKAC 2 cut(s) 80, 470
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.