Rw0G022980

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01067
OG0000286
Physical Location & Seq
Reverse (-)
5091 .. 5480
390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G022980.1

Sequence Viewer

Length: 390 bp
ATGGAAGAACTACATGCAGCTGCTTTAGCATACTACGACAATGGCACTCAACAGCTTCGGGATCTGGCAGGGTCTTTCTTCCGATCTATGGACACCAACGGGGATGGTCAAATCAGCTGCTTGGAGTTCAATGACTTCCTCCAGCAAAGTGGCTACAACTGGATTATTAACGATCCAAACTTCTTCAAAAGACTTGACAGCAATGGCGATGGTGGATTGGATTTTGGGGAAGTGCTCACTTTCTATTATATCATTAAAACGAGGTACATCAAGTGCCAAGGGTATCGGTGCGGTGTACACCTCTGTGGTTTGTATTTCACTTGCGTTGCTTGCTTTGACGGAGCTCATGAGCATCGCTCCACCTTTGATCTCTGTCTTGCCTGCAGGGCC
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

130

Amino Acids

14.78

Weight (kDa)

4.97

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 291
AclWI GGATC 2 cut(s) 69, 167
AfaI GTAC 2 cut(s) 266, 297
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 2 cut(s) 130, 187
AleI CACNNNNGTG 1 cut(s) 303
AluBI AGCT 4 cut(s) 20, 55, 117, 344
AluI AGCT 4 cut(s) 20, 55, 117, 344
Alw21I GWGCWC 2 cut(s) 237, 346
AlwI GGATC 2 cut(s) 69, 167
ApeKI GCWGC 3 cut(s) 17, 20, 117
AspS9I GGNCC 1 cut(s) 387
BanII GRGCYC 1 cut(s) 346
Bbv12I GWGCWC 2 cut(s) 237, 346
BbvI GCAGC 3 cut(s) 7, 29, 104
BccI CCATC 2 cut(s) 98, 203
BfmI CTRYAG 1 cut(s) 382
BglI GCCNNNNNGGC 1 cut(s) 386
BisI GCNGC 3 cut(s) 18, 21, 118
BlsI GCNGC 3 cut(s) 19, 22, 119
BmgT120I GGNCC 1 cut(s) 387
BmsI GCATC 1 cut(s) 361
BplI GAGNNNNNCTC 2 cut(s) 341, 373
BpmI CTGGAG 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 277
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 208
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 1 cut(s) 109
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMI GCAATG 1 cut(s) 208
BseNI ACTGG 1 cut(s) 164
BseXI GCAGC 3 cut(s) 7, 29, 104
BshFI GGCC 1 cut(s) 389
BsiHKAI GWGCWC 2 cut(s) 237, 346
BslI CCNNNNNNNGG 1 cut(s) 88
BsnI GGCC 1 cut(s) 389
Bsp1286I GDGCHC 2 cut(s) 237, 346
Bsp1407I TGTACA 1 cut(s) 295
Bsp143I GATC 4 cut(s) 61, 83, 172, 367
BspACI CCGC 1 cut(s) 291
BspANI GGCC 1 cut(s) 389
BspHI TCATGA 1 cut(s) 346
BspMAI CTGCAG 1 cut(s) 386
BspPI GGATC 2 cut(s) 69, 167
BsrDI GCAATG 1 cut(s) 208
BsrGI TGTACA 1 cut(s) 295
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 4 cut(s) 61, 83, 172, 367
BssT1I CCWWGG 1 cut(s) 277
BstAUI TGTACA 1 cut(s) 295
BstC8I GCNNGC 2 cut(s) 331, 382
BstF5I GGATG 1 cut(s) 109
BstKTI GATC 4 cut(s) 64, 86, 175, 370
BstMBI GATC 4 cut(s) 61, 83, 172, 367
BstMWI GCNNNNNNNGC 3 cut(s) 26, 330, 386
BstNSI RCATGY 1 cut(s) 17
BstSFI CTRYAG 1 cut(s) 382
BstV1I GCAGC 3 cut(s) 7, 29, 104
BstX2I RGATCY 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 149
BstYI RGATCY 1 cut(s) 61
BsuRI GGCC 1 cut(s) 389
BtgZI GCGATG 2 cut(s) 222, 338
BtsCI GGATG 1 cut(s) 109
Cac8I GCNNGC 2 cut(s) 331, 382
CciI TCATGA 1 cut(s) 346
Cfr13I GGNCC 1 cut(s) 387
Csp6I GTAC 2 cut(s) 265, 296
CviAII CATG 2 cut(s) 14, 347
CviJI RGCY 6 cut(s) 20, 55, 117, 153, 344, 389
CviKI_1 RGCY 6 cut(s) 20, 55, 117, 153, 344, 389
CviQI GTAC 2 cut(s) 265, 296
DpnI GATC 4 cut(s) 63, 85, 174, 369
DpnII GATC 4 cut(s) 61, 83, 172, 367
Ecl136II GAGCTC 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 346
Eco53kI GAGCTC 1 cut(s) 344
EcoICRI GAGCTC 1 cut(s) 344
EcoT14I CCWWGG 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 2 cut(s) 17, 350
FaiI YATR 5 cut(s) 15, 31, 89, 249, 348
FatI CATG 2 cut(s) 13, 346
Fnu4HI GCNGC 3 cut(s) 18, 21, 118
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 346
Fsp4HI GCNGC 3 cut(s) 18, 21, 118
GluI GCNGC 3 cut(s) 18, 21, 118
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 1 cut(s) 389
Hin1II CATG 2 cut(s) 17, 350
Hpy166II GTNNAC 2 cut(s) 296, 298
Hpy188I TCNGA 1 cut(s) 83
Hpy188III TCNNGA 2 cut(s) 59, 347
Hpy8I GTNNAC 2 cut(s) 296, 298
HpyCH4V TGCA 2 cut(s) 17, 384
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 330, 386
Hsp92II CATG 2 cut(s) 17, 350
Kzo9I GATC 4 cut(s) 61, 83, 172, 367
LmnI GCTCC 2 cut(s) 341, 362
LpnPI CCDG 5 cut(s) 50, 54, 145, 155, 370
Lsp1109I GCAGC 3 cut(s) 7, 29, 104
LweI GCATC 1 cut(s) 361
MalI GATC 4 cut(s) 63, 85, 174, 369
MboI GATC 4 cut(s) 61, 83, 172, 367
MboII GAAGA 3 cut(s) 17, 70, 175
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 2 cut(s) 237, 346
MnlI CCTC 3 cut(s) 149, 255, 311
MseI TTAA 2 cut(s) 168, 255
MslI CAYNNNNRTG 1 cut(s) 303
MspA1I CMGCKG 2 cut(s) 20, 117
MwoI GCNNNNNNNGC 3 cut(s) 26, 330, 386
NdeII GATC 4 cut(s) 61, 83, 172, 367
NlaIII CATG 2 cut(s) 17, 350
NspI RCATGY 1 cut(s) 17
OliI CACNNNNGTG 1 cut(s) 303
PagI TCATGA 1 cut(s) 346
PkrI GCNGC 3 cut(s) 19, 22, 119
Psp124BI GAGCTC 1 cut(s) 346
PspPI GGNCC 1 cut(s) 387
PstI CTGCAG 1 cut(s) 386
PsuI RGATCY 1 cut(s) 61
PvuII CAGCTG 2 cut(s) 20, 117
RsaI GTAC 2 cut(s) 266, 297
RsaNI GTAC 2 cut(s) 265, 296
RseI CAYNNNNRTG 1 cut(s) 303
SacI GAGCTC 1 cut(s) 346
SaqAI TTAA 2 cut(s) 168, 255
SatI GCNGC 3 cut(s) 18, 21, 118
Sau3AI GATC 4 cut(s) 61, 83, 172, 367
Sau96I GGNCC 1 cut(s) 387
SbfI CCTGCAGG 1 cut(s) 386
SdaI CCTGCAGG 1 cut(s) 386
SduI GDGCHC 2 cut(s) 237, 346
SetI ASST 7 cut(s) 22, 57, 119, 266, 303, 346, 365
SfaNI GCATC 1 cut(s) 361
SfcI CTRYAG 1 cut(s) 382
SmiMI CAYNNNNRTG 1 cut(s) 303
Sse8387I CCTGCAGG 1 cut(s) 386
SsiI CCGC 1 cut(s) 291
SstI GAGCTC 1 cut(s) 346
StyI CCWWGG 1 cut(s) 277
TatI WGTACW 1 cut(s) 295
Tru1I TTAA 2 cut(s) 168, 255
Tru9I TTAA 2 cut(s) 168, 255
TseI GCWGC 3 cut(s) 17, 20, 117
TspGWI ACGGA 1 cut(s) 354
XceI RCATGY 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.