RchiOBHm_Chr4g0395801

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000286
Physical Location & Seq
Forward (+)
11839042 .. 11839626
585 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36822

Sequence Viewer

Length: 585 bp
ATGGCAATGGAAGAACTACATGCAGCTGCTTTAGCATACTACGACAATGGCACTCAACAGCTTCGGGATCTGGCAGGGTCTTTCTTCCGATCTATGGACACCAACGGGGATGGTCAAATCAGCTGCTTGGAGTTCAATGACTTCCTCCAGCAAAGTGGCTACAACTGGATTATTAACGATCCAAACTTCTTCAAAAGACTTGACCGCAATGGCGATGGTGGATTGGATTTTGGGGAAGTTCTCACTTTCTATTATATCATTAAAACGAGGTACATCAAGTGCCAAGGGTATCGGTGCGGTGTACACCTCTGTGGTTTGTATTTCACTTGCGTTGCTTGCTTTGACGGATCTCATGAGCATTGCTCCACCTTTGATCTCTGTCCTGCCTGCTATCGCAATCGGAGTTACTATCACCACCAGCACCACACATACTTCTTGGATAATCATGTATTGCTGCGCTCCAAACGAGGCCTCTCTCCATATGCTCCACCAAATCTCAACATGGTACATAATTGTATTACATTCTTTATCACATTTATGCGCCTTGACCTTCACTTTGTTCTACATTTGTACGTAGACCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

194

Amino Acids

22.64

Weight (kDa)

6.28

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 576
AciI CCGC 2 cut(s) 205, 297
AclWI GGATC 3 cut(s) 75, 173, 355
AfaI GTAC 4 cut(s) 272, 303, 507, 572
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 2 cut(s) 136, 193
AleI CACNNNNGTG 1 cut(s) 309
AluBI AGCT 3 cut(s) 26, 61, 123
AluI AGCT 3 cut(s) 26, 61, 123
AlwI GGATC 3 cut(s) 75, 173, 355
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 4 cut(s) 23, 26, 123, 454
ArsI GACNNNNNNTTYG 2 cut(s) 539, 571
AspLEI GCGC 2 cut(s) 459, 543
AsuHPI GGTGA 1 cut(s) 404
BbvI GCAGC 4 cut(s) 13, 35, 110, 441
BccI CCATC 2 cut(s) 104, 209
BisI GCNGC 4 cut(s) 24, 27, 124, 455
BlsI GCNGC 4 cut(s) 25, 28, 125, 456
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 1 cut(s) 131
BsaAI YACGTR 1 cut(s) 574
BsaJI CCNNGG 1 cut(s) 283
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 3 cut(s) 12, 214, 358
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 3 cut(s) 12, 214, 358
BseNI ACTGG 1 cut(s) 170
BseXI GCAGC 4 cut(s) 13, 35, 110, 441
BshFI GGCC 1 cut(s) 471
BslI CCNNNNNNNGG 1 cut(s) 94
BsnI GGCC 1 cut(s) 471
Bsp1407I TGTACA 1 cut(s) 301
Bsp143I GATC 5 cut(s) 67, 89, 178, 347, 373
BspACI CCGC 2 cut(s) 205, 297
BspANI GGCC 1 cut(s) 471
BspHI TCATGA 1 cut(s) 352
BspPI GGATC 3 cut(s) 75, 173, 355
BsrDI GCAATG 3 cut(s) 12, 214, 358
BsrGI TGTACA 1 cut(s) 301
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 5 cut(s) 67, 89, 178, 347, 373
BssT1I CCWWGG 1 cut(s) 283
BstAUI TGTACA 1 cut(s) 301
BstBAI YACGTR 1 cut(s) 574
BstC8I GCNNGC 2 cut(s) 337, 388
BstF5I GGATG 1 cut(s) 115
BstHHI GCGC 2 cut(s) 459, 543
BstKTI GATC 5 cut(s) 70, 92, 181, 350, 376
BstMBI GATC 5 cut(s) 67, 89, 178, 347, 373
BstMWI GCNNNNNNNGC 2 cut(s) 32, 336
BstNSI RCATGY 1 cut(s) 23
BstSNI TACGTA 1 cut(s) 574
BstV1I GCAGC 4 cut(s) 13, 35, 110, 441
BstX2I RGATCY 2 cut(s) 67, 347
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 2 cut(s) 67, 347
BsuRI GGCC 1 cut(s) 471
BtgZI GCGATG 1 cut(s) 228
BtsCI GGATG 1 cut(s) 115
Cac8I GCNNGC 2 cut(s) 337, 388
CciI TCATGA 1 cut(s) 352
CfoI GCGC 2 cut(s) 459, 543
Csp6I GTAC 4 cut(s) 271, 302, 506, 571
CviAII CATG 4 cut(s) 20, 353, 446, 502
CviJI RGCY 5 cut(s) 26, 61, 123, 159, 471
CviKI_1 RGCY 5 cut(s) 26, 61, 123, 159, 471
CviQI GTAC 4 cut(s) 271, 302, 506, 571
DpnI GATC 5 cut(s) 69, 91, 180, 349, 375
DpnII GATC 5 cut(s) 67, 89, 178, 347, 373
Eco105I TACGTA 1 cut(s) 574
Eco130I CCWWGG 1 cut(s) 283
Eco147I AGGCCT 1 cut(s) 471
EcoT14I CCWWGG 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 4 cut(s) 23, 356, 449, 505
FatI CATG 4 cut(s) 19, 352, 445, 501
FauNDI CATATG 1 cut(s) 481
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 4 cut(s) 24, 27, 124, 455
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 4 cut(s) 24, 27, 124, 455
GlaI GCGC 2 cut(s) 458, 542
GluI GCNGC 4 cut(s) 24, 27, 124, 455
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 1 cut(s) 471
HhaI GCGC 2 cut(s) 459, 543
Hin1II CATG 4 cut(s) 23, 356, 449, 505
Hin6I GCGC 2 cut(s) 457, 541
HinP1I GCGC 2 cut(s) 457, 541
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 3 cut(s) 302, 304, 577
Hpy188I TCNGA 2 cut(s) 89, 402
Hpy188III TCNNGA 2 cut(s) 65, 353
Hpy8I GTNNAC 3 cut(s) 302, 304, 577
HpyAV CCTTC 1 cut(s) 560
HpyCH4IV ACGT 1 cut(s) 573
HpyCH4V TGCA 1 cut(s) 23
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 336
HpySE526I ACGT 1 cut(s) 573
Hsp92II CATG 4 cut(s) 23, 356, 449, 505
HspAI GCGC 2 cut(s) 457, 541
Kzo9I GATC 5 cut(s) 67, 89, 178, 347, 373
LmnI GCTCC 3 cut(s) 368, 464, 490
LpnPI CCDG 7 cut(s) 56, 60, 151, 161, 396, 400, 431
Lsp1109I GCAGC 4 cut(s) 13, 35, 110, 441
MaeII ACGT 1 cut(s) 573
MaeIII GTNAC 1 cut(s) 404
MalI GATC 5 cut(s) 69, 91, 180, 349, 375
MboI GATC 5 cut(s) 67, 89, 178, 347, 373
MboII GAAGA 3 cut(s) 23, 76, 181
MflI RGATCY 2 cut(s) 67, 347
MluCI AATT 1 cut(s) 511
MnlI CCTC 5 cut(s) 155, 261, 317, 461, 482
MseI TTAA 2 cut(s) 174, 261
MslI CAYNNNNRTG 2 cut(s) 309, 536
MspA1I CMGCKG 2 cut(s) 26, 123
MwoI GCNNNNNNNGC 2 cut(s) 32, 336
NdeI CATATG 1 cut(s) 481
NdeII GATC 5 cut(s) 67, 89, 178, 347, 373
NlaIII CATG 4 cut(s) 23, 356, 449, 505
NspI RCATGY 1 cut(s) 23
OliI CACNNNNGTG 1 cut(s) 309
PagI TCATGA 1 cut(s) 352
PceI AGGCCT 1 cut(s) 471
PkrI GCNGC 4 cut(s) 25, 28, 125, 456
Ppu21I YACGTR 1 cut(s) 574
PsuI RGATCY 2 cut(s) 67, 347
PvuII CAGCTG 2 cut(s) 26, 123
RsaI GTAC 4 cut(s) 272, 303, 507, 572
RsaNI GTAC 4 cut(s) 271, 302, 506, 571
RseI CAYNNNNRTG 2 cut(s) 309, 536
SaqAI TTAA 2 cut(s) 174, 261
SatI GCNGC 4 cut(s) 24, 27, 124, 455
Sau3AI GATC 5 cut(s) 67, 89, 178, 347, 373
SetI ASST 9 cut(s) 28, 63, 125, 272, 309, 371, 552, 576, 582
SmiMI CAYNNNNRTG 2 cut(s) 309, 536
SnaBI TACGTA 1 cut(s) 574
Sse9I AATT 1 cut(s) 511
SseBI AGGCCT 1 cut(s) 471
SsiI CCGC 2 cut(s) 205, 297
StuI AGGCCT 1 cut(s) 471
StyI CCWWGG 1 cut(s) 283
TaiI ACGT 1 cut(s) 576
TasI AATT 1 cut(s) 511
TatI WGTACW 1 cut(s) 301
Tru1I TTAA 2 cut(s) 174, 261
Tru9I TTAA 2 cut(s) 174, 261
TseI GCWGC 4 cut(s) 23, 26, 123, 454
TspGWI ACGGA 1 cut(s) 360
XceI RCATGY 1 cut(s) 23
XmiI GTMKAC 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.