Rh4DG056800

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000286
Physical Location & Seq
Forward (+)
8755204 .. 8796773
41570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG056800.1

Sequence Viewer

Length: 534 bp
ATGGCACTCAACAGCTTCGGGATCTGGCAGGGTCTTTCTTCCGATCTATGGACACCAACGGTGATGGTCAAATCGATCAGCTGCTTGGAATTCAATGACTTCCTCCAGCAAAGCGGCTACAACTGGATTATTAACGACCCAAACTTCTTCAAAAGACTCGACCGCAATGGCGATAATGGATTGGATTTTGGGGAAGTTCTCACATTCTACTACATCATTAAAACGAGGTACATCCGATGCCAAGGGTACCAGTGCGGCGTACACCTCTGCGGTTTGTATTTCACTTGCGGTGGTTGCTTTGACGAGGCTCATGAACATCGCTCCACCTTTGATCTATGTCCTGCCTGCTATCGCAATTGGAATTACTATCATCACCAGCACCACACTTCTTGGATAATCATGTATTGCTGCGCTCCAAACGAGGCCTCTCTCCATATGCTCAACCAGATCTCAACATGTTGGTTCCAGGTATTTCGCGTGTTTGAGATGGCTCTTGCTGCTGCAAGTGTGGCTGCTAATTGTACAATGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

177

Amino Acids

20.58

Weight (kDa)

6.01

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 246
AccII CGCG 1 cut(s) 477
AciI CCGC 5 cut(s) 114, 163, 255, 270, 288
AclWI GGATC 1 cut(s) 29
AcsI RAATTY 1 cut(s) 89
AfaI GTAC 4 cut(s) 230, 248, 261, 523
AfiI CCNNNNNNNGG 1 cut(s) 48
AflIII ACRYGT 1 cut(s) 455
AgsI TTSAA 2 cut(s) 94, 151
AjnI CCWGG 1 cut(s) 465
AluBI AGCT 2 cut(s) 15, 81
AluI AGCT 2 cut(s) 15, 81
AlwI GGATC 1 cut(s) 29
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 5 cut(s) 81, 408, 497, 500, 512
ApoI RAATTY 1 cut(s) 89
Asp718I GGTACC 1 cut(s) 246
AspLEI GCGC 1 cut(s) 413
AsuHPI GGTGA 2 cut(s) 73, 365
BanI GGYRCC 1 cut(s) 246
BbvI GCAGC 5 cut(s) 68, 395, 484, 487, 499
BccI CCATC 2 cut(s) 58, 481
BciT130I CCWGG 1 cut(s) 467
BglII AGATCT 1 cut(s) 447
BisI GCNGC 7 cut(s) 82, 115, 256, 409, 498, 501, 513
BlsI GCNGC 7 cut(s) 83, 116, 257, 410, 499, 502, 514
Bme1390I CCNGG 1 cut(s) 467
BmiI GGNNCC 2 cut(s) 248, 464
BmrFI CCNGG 1 cut(s) 467
BmsI GCATC 1 cut(s) 227
BpmI CTGGAG 1 cut(s) 89
Bsa29I ATCGAT 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 241
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse1I ACTGG 2 cut(s) 128, 250
Bse3DI GCAATG 1 cut(s) 172
BseBI CCWGG 1 cut(s) 467
BseCI ATCGAT 1 cut(s) 74
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 1 cut(s) 231
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMI GCAATG 1 cut(s) 172
BseNI ACTGG 2 cut(s) 128, 250
BseXI GCAGC 5 cut(s) 68, 395, 484, 487, 499
Bsh1236I CGCG 1 cut(s) 477
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 1 cut(s) 425
BshNI GGYRCC 1 cut(s) 246
BshVI ATCGAT 1 cut(s) 74
BsiEI CGRYCG 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 48
BsnI GGCC 1 cut(s) 425
Bsp1407I TGTACA 1 cut(s) 521
Bsp143I GATC 5 cut(s) 21, 43, 75, 331, 447
BspACI CCGC 5 cut(s) 114, 163, 255, 270, 288
BspANI GGCC 1 cut(s) 425
BspDI ATCGAT 1 cut(s) 74
BspFNI CGCG 1 cut(s) 477
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 2 cut(s) 248, 464
BspPI GGATC 1 cut(s) 29
BspT107I GGYRCC 1 cut(s) 246
BsrDI GCAATG 1 cut(s) 172
BsrGI TGTACA 1 cut(s) 521
BsrI ACTGG 2 cut(s) 128, 250
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 5 cut(s) 21, 43, 75, 331, 447
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 1 cut(s) 467
Bst4CI ACNGT 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 346
BstF5I GGATG 1 cut(s) 231
BstFNI CGCG 1 cut(s) 477
BstHHI GCGC 1 cut(s) 413
BstKTI GATC 5 cut(s) 24, 46, 78, 334, 450
BstMBI GATC 5 cut(s) 21, 43, 75, 331, 447
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 3 cut(s) 294, 497, 509
BstNI CCWGG 1 cut(s) 467
BstNSI RCATGY 1 cut(s) 459
BstSCI CCNGG 1 cut(s) 465
BstUI CGCG 1 cut(s) 477
BstV1I GCAGC 5 cut(s) 68, 395, 484, 487, 499
BstX2I RGATCY 2 cut(s) 21, 447
BstYI RGATCY 2 cut(s) 21, 447
Bsu15I ATCGAT 1 cut(s) 74
BsuRI GGCC 1 cut(s) 425
BsuTUI ATCGAT 1 cut(s) 74
BtgZI GCGATG 1 cut(s) 302
BtsCI GGATG 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 257
Cac8I GCNNGC 1 cut(s) 346
CciI TCATGA 1 cut(s) 310
CfoI GCGC 1 cut(s) 413
ClaI ATCGAT 1 cut(s) 74
Csp6I GTAC 4 cut(s) 229, 247, 260, 522
CspCI CAANNNNNGTGG 2 cut(s) 371, 406
CviAII CATG 3 cut(s) 311, 400, 456
CviJI RGCY 7 cut(s) 15, 81, 117, 308, 425, 491, 512
CviKI_1 RGCY 7 cut(s) 15, 81, 117, 308, 425, 491, 512
CviQI GTAC 4 cut(s) 229, 247, 260, 522
DpnI GATC 5 cut(s) 23, 45, 77, 333, 449
DpnII GATC 5 cut(s) 21, 43, 75, 331, 447
Eco130I CCWWGG 1 cut(s) 241
Eco147I AGGCCT 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 465
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 3 cut(s) 314, 403, 459
FaiI YATR 7 cut(s) 49, 312, 337, 401, 435, 437, 457
FatI CATG 3 cut(s) 310, 399, 455
FauNDI CATATG 1 cut(s) 435
Fnu4HI GCNGC 7 cut(s) 82, 115, 256, 409, 498, 501, 513
FokI GGATG 1 cut(s) 218
Fsp4HI GCNGC 7 cut(s) 82, 115, 256, 409, 498, 501, 513
GlaI GCGC 1 cut(s) 412
GluI GCNGC 7 cut(s) 82, 115, 256, 409, 498, 501, 513
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 1 cut(s) 425
HhaI GCGC 1 cut(s) 413
Hin1II CATG 3 cut(s) 314, 403, 459
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HinfI GANTC 1 cut(s) 156
HphI GGTGA 2 cut(s) 73, 365
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 2 cut(s) 43, 236
Hpy188III TCNNGA 2 cut(s) 19, 311
Hpy8I GTNNAC 1 cut(s) 262
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4V TGCA 1 cut(s) 503
HpyF10VI GCNNNNNNNGC 3 cut(s) 294, 497, 509
Hsp92II CATG 3 cut(s) 314, 403, 459
HspAI GCGC 1 cut(s) 411
KpnI GGTACC 1 cut(s) 250
Kzo9I GATC 5 cut(s) 21, 43, 75, 331, 447
LmnI GCTCC 2 cut(s) 326, 418
Lsp1109I GCAGC 5 cut(s) 68, 395, 484, 487, 499
LweI GCATC 1 cut(s) 227
MalI GATC 5 cut(s) 23, 45, 77, 333, 449
MboI GATC 5 cut(s) 21, 43, 75, 331, 447
MboII GAAGA 2 cut(s) 30, 139
MfeI CAATTG 1 cut(s) 355
MflI RGATCY 2 cut(s) 21, 447
MluCI AATT 4 cut(s) 89, 355, 361, 517
MlyI GAGTC 1 cut(s) 150
MnlI CCTC 6 cut(s) 113, 219, 275, 298, 415, 436
MseI TTAA 2 cut(s) 132, 219
MspA1I CMGCKG 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 467
MunI CAATTG 1 cut(s) 355
MvaI CCWGG 1 cut(s) 467
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 3 cut(s) 294, 497, 509
NdeI CATATG 1 cut(s) 435
NdeII GATC 5 cut(s) 21, 43, 75, 331, 447
NlaIII CATG 3 cut(s) 314, 403, 459
NlaIV GGNNCC 2 cut(s) 248, 464
NspI RCATGY 1 cut(s) 459
PagI TCATGA 1 cut(s) 310
PceI AGGCCT 1 cut(s) 425
PciI ACATGT 1 cut(s) 455
PkrI GCNGC 7 cut(s) 83, 116, 257, 410, 499, 502, 514
PleI GAGTC 1 cut(s) 150
PpsI GAGTC 1 cut(s) 150
PscI ACATGT 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 465
PspGI CCWGG 1 cut(s) 465
PspN4I GGNNCC 2 cut(s) 248, 464
PsuI RGATCY 2 cut(s) 21, 447
PvuII CAGCTG 1 cut(s) 81
RsaI GTAC 4 cut(s) 230, 248, 261, 523
RsaNI GTAC 4 cut(s) 229, 247, 260, 522
SaqAI TTAA 2 cut(s) 132, 219
SatI GCNGC 7 cut(s) 82, 115, 256, 409, 498, 501, 513
Sau3AI GATC 5 cut(s) 21, 43, 75, 331, 447
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 1 cut(s) 467
SetI ASST 6 cut(s) 17, 83, 230, 267, 329, 471
SfaNI GCATC 1 cut(s) 227
Sse9I AATT 4 cut(s) 89, 355, 361, 517
SseBI AGGCCT 1 cut(s) 425
SsiI CCGC 5 cut(s) 114, 163, 255, 270, 288
StuI AGGCCT 1 cut(s) 425
StyD4I CCNGG 1 cut(s) 465
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 1 cut(s) 61
TaqI TCGA 2 cut(s) 74, 159
TasI AATT 4 cut(s) 89, 355, 361, 517
TatI WGTACW 1 cut(s) 521
TauI GCSGC 2 cut(s) 117, 258
Tru1I TTAA 2 cut(s) 132, 219
Tru9I TTAA 2 cut(s) 132, 219
TscAI CASTG 1 cut(s) 257
TseI GCWGC 5 cut(s) 81, 408, 497, 500, 512
TspDTI ATGAA 1 cut(s) 327
TspRI CASTG 1 cut(s) 257
XapI RAATTY 1 cut(s) 89
XceI RCATGY 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.