RchiOBHm_Chr4g0395571

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000286
Physical Location & Seq
Forward (+)
11332528 .. 11333055
528 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36801

Sequence Viewer

Length: 528 bp
ATGCAAGAAATACACAACGCTGCTTTAGCTTATTACCATAACTTATCAGATGAGCTAAAGCAGAAGGCATCTGAAAAATTCAAAGAAATGGATTCGAATGATAATGGCACAATAAGCATCAAGGAGTTCCGGCGGAGTTTGGGCAGCTCCGAGATCAAGCGCTGCTTCAAGTTGCTGGACAAAACCGGAGACGGCAAGTTGGATTTCAACGAATTCATCACTCTTTACTACCTCGTGGAGAGTGGGAGAGGGGTTTTCTGTGACGGCCATGGATGTAAGGCCTCCCCTTTTCTCAACGGACTGTATTTTACTTGCGTTGAGTGCTTCCATAACAACGAAGCTGCAACGTTCGATCTCTGCACCTCCTGCTACCGCGATGGAAAGTATGTTCACAACCATGCCAGCTTTTTGGATAATTACGTCTTTTTGCGTTCCAAGGCTGCTTGTGAATCCACAACACCAACGGAAGATGATCATCATGAGGTAACATATGATCAGTTTATATTAGAAACAGAAAATGATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

175

Amino Acids

20.05

Weight (kDa)

5.1

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 21 - 77 6.5e-11 EF-hand domain pair
EF-hand_1 PF00036 51 - 75 2.6e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 375
AciI CCGC 2 cut(s) 133, 373
AclI AACGTT 1 cut(s) 347
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 2 cut(s) 77, 212
AfeI AGCGCT 1 cut(s) 161
AgsI TTSAA 3 cut(s) 82, 169, 208
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
AluBI AGCT 5 cut(s) 29, 55, 147, 341, 405
AluI AGCT 5 cut(s) 29, 55, 147, 341, 405
Alw26I GTCTC 1 cut(s) 183
Aor51HI AGCGCT 1 cut(s) 161
AoxI GGCC 2 cut(s) 265, 279
ApeKI GCWGC 5 cut(s) 20, 144, 162, 341, 440
ApoI RAATTY 2 cut(s) 77, 212
AspLEI GCGC 1 cut(s) 162
AsuII TTCGAA 1 cut(s) 95
BauI CACGAG 1 cut(s) 233
BbvI GCAGC 5 cut(s) 7, 149, 156, 328, 427
BccI CCATC 1 cut(s) 371
BceAI ACGGC 2 cut(s) 208, 280
BclI TGATCA 2 cut(s) 472, 493
BcoDI GTCTC 1 cut(s) 183
BfoI RGCGCY 1 cut(s) 163
BisI GCNGC 5 cut(s) 21, 145, 163, 342, 441
BlsI GCNGC 5 cut(s) 22, 146, 164, 343, 442
BmsI GCATC 2 cut(s) 77, 126
Bpu14I TTCGAA 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 474
BsaJI CCNNGG 2 cut(s) 268, 435
BsaWI WCCGGW 1 cut(s) 185
Bse8I GATNNNNATC 1 cut(s) 474
BseDI CCNNGG 2 cut(s) 268, 435
BseGI GGATG 1 cut(s) 278
BseJI GATNNNNATC 1 cut(s) 474
BseXI GCAGC 5 cut(s) 7, 149, 156, 328, 427
BsgI GTGCAG 1 cut(s) 343
Bsh1236I CGCG 1 cut(s) 375
BshFI GGCC 2 cut(s) 267, 281
BsiSI CCGG 2 cut(s) 130, 186
BsmAI GTCTC 1 cut(s) 183
BsmBI CGTCTC 1 cut(s) 183
BsnI GGCC 2 cut(s) 267, 281
Bsp119I TTCGAA 1 cut(s) 95
Bsp143I GATC 4 cut(s) 153, 352, 472, 493
Bsp19I CCATGG 1 cut(s) 268
BspACI CCGC 2 cut(s) 133, 373
BspANI GGCC 2 cut(s) 267, 281
BspFNI CGCG 1 cut(s) 375
BspHI TCATGA 1 cut(s) 478
BspT104I TTCGAA 1 cut(s) 95
BssECI CCNNGG 2 cut(s) 268, 435
BssMI GATC 4 cut(s) 153, 352, 472, 493
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 2 cut(s) 268, 435
Bst2BI CACGAG 1 cut(s) 233
Bst4CI ACNGT 1 cut(s) 303
BstAPI GCANNNNNTGC 1 cut(s) 366
BstBI TTCGAA 1 cut(s) 95
BstC8I GCNNGC 1 cut(s) 403
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 1 cut(s) 278
BstFNI CGCG 1 cut(s) 375
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 4 cut(s) 156, 355, 475, 496
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 4 cut(s) 153, 352, 472, 493
BstMWI GCNNNNNNNGC 4 cut(s) 26, 114, 321, 366
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 5 cut(s) 7, 149, 156, 328, 427
BstXI CCANNNNNNTGG 1 cut(s) 409
BsuRI GGCC 2 cut(s) 267, 281
BtgI CCRYGG 1 cut(s) 268
BtgZI GCGATG 1 cut(s) 390
BtsCI GGATG 1 cut(s) 278
Cac8I GCNNGC 1 cut(s) 403
CciI TCATGA 1 cut(s) 478
CfoI GCGC 1 cut(s) 162
CviAII CATG 3 cut(s) 269, 398, 479
CviJI RGCY 8 cut(s) 29, 55, 147, 267, 281, 341, 405, 440
CviKI_1 RGCY 8 cut(s) 29, 55, 147, 267, 281, 341, 405, 440
DpnI GATC 4 cut(s) 155, 354, 474, 495
DpnII GATC 4 cut(s) 153, 352, 472, 493
EaeI YGGCCR 1 cut(s) 265
EciI GGCGGA 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 268, 435
Eco147I AGGCCT 1 cut(s) 281
Eco47III AGCGCT 1 cut(s) 161
EcoRI GAATTC 1 cut(s) 212
EcoT14I CCWWGG 2 cut(s) 268, 435
ErhI CCWWGG 2 cut(s) 268, 435
Esp3I CGTCTC 1 cut(s) 183
FaeI CATG 3 cut(s) 272, 401, 482
FaiI YATR 9 cut(s) 39, 270, 330, 387, 399, 480, 490, 492, 503
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FatI CATG 3 cut(s) 268, 397, 478
FauNDI CATATG 1 cut(s) 490
FbaI TGATCA 2 cut(s) 472, 493
Fnu4HI GCNGC 5 cut(s) 21, 145, 163, 342, 441
FokI GGATG 1 cut(s) 285
Fsp4HI GCNGC 5 cut(s) 21, 145, 163, 342, 441
GlaI GCGC 1 cut(s) 161
GluI GCNGC 5 cut(s) 21, 145, 163, 342, 441
HaeII RGCGCY 1 cut(s) 163
HaeIII GGCC 2 cut(s) 267, 281
HapII CCGG 2 cut(s) 130, 186
HhaI GCGC 1 cut(s) 162
Hin1II CATG 3 cut(s) 272, 401, 482
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HinfI GANTC 2 cut(s) 92, 449
HpaII CCGG 2 cut(s) 130, 186
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 3 cut(s) 49, 73, 151
Hpy188III TCNNGA 1 cut(s) 479
Hpy8I GTNNAC 1 cut(s) 391
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 1 cut(s) 303
HpyCH4IV ACGT 2 cut(s) 347, 420
HpyCH4V TGCA 3 cut(s) 4, 344, 360
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 114, 321, 366
HpySE526I ACGT 2 cut(s) 347, 420
Hsp92II CATG 3 cut(s) 272, 401, 482
HspAI GCGC 1 cut(s) 160
Ksp22I TGATCA 2 cut(s) 472, 493
Kzo9I GATC 4 cut(s) 153, 352, 472, 493
LmnI GCTCC 1 cut(s) 152
LpnPI CCDG 5 cut(s) 143, 161, 199, 379, 415
Lsp1109I GCAGC 5 cut(s) 7, 149, 156, 328, 427
LweI GCATC 2 cut(s) 77, 126
MaeII ACGT 2 cut(s) 347, 420
MaeIII GTNAC 2 cut(s) 260, 484
MalI GATC 4 cut(s) 155, 354, 474, 495
MboI GATC 4 cut(s) 153, 352, 472, 493
MboII GAAGA 1 cut(s) 479
MluCI AATT 3 cut(s) 77, 212, 415
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 5 cut(s) 242, 242, 292, 373, 475
MseI TTAA 1 cut(s) 526
MslI CAYNNNNRTG 1 cut(s) 396
MspI CCGG 2 cut(s) 130, 186
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 4 cut(s) 26, 114, 321, 366
NcoI CCATGG 1 cut(s) 268
NdeI CATATG 1 cut(s) 490
NdeII GATC 4 cut(s) 153, 352, 472, 493
NlaIII CATG 3 cut(s) 272, 401, 482
NmuCI GTSAC 1 cut(s) 260
NspV TTCGAA 1 cut(s) 95
PagI TCATGA 1 cut(s) 478
PceI AGGCCT 1 cut(s) 281
PfeI GAWTC 2 cut(s) 92, 449
PkrI GCNGC 5 cut(s) 22, 146, 164, 343, 442
Psp1406I AACGTT 1 cut(s) 347
RseI CAYNNNNRTG 1 cut(s) 396
SaqAI TTAA 1 cut(s) 526
SatI GCNGC 5 cut(s) 21, 145, 163, 342, 441
Sau3AI GATC 4 cut(s) 153, 352, 472, 493
SfaNI GCATC 2 cut(s) 77, 126
SfuI TTCGAA 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 396
Sse9I AATT 3 cut(s) 77, 212, 415
SseBI AGGCCT 1 cut(s) 281
SsiI CCGC 2 cut(s) 133, 373
StuI AGGCCT 1 cut(s) 281
StyI CCWWGG 2 cut(s) 268, 435
TaaI ACNGT 1 cut(s) 303
TaiI ACGT 2 cut(s) 350, 423
TaqI TCGA 2 cut(s) 95, 351
TasI AATT 3 cut(s) 77, 212, 415
TfiI GAWTC 2 cut(s) 92, 449
Tru1I TTAA 1 cut(s) 526
Tru9I TTAA 1 cut(s) 526
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 5 cut(s) 20, 144, 162, 341, 440
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 1 cut(s) 205
TspGWI ACGGA 2 cut(s) 312, 479
XapI RAATTY 2 cut(s) 77, 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.