Rh4DG056100

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000286
Physical Location & Seq
Forward (+)
8679131 .. 8680310
1180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG056100.1

Sequence Viewer

Length: 531 bp
ATGGGAAGCTCTTTCGACAATGACTCCGTGATCATGAAGAGAAGCTTCAAGGAGCTGGACAAGAACGGAGACGGCAAGTTGGATTTCAACGAATACATCACTCTTTACTATCTTGTAGAGAGCGGTAGGGTGCTGATTTATTGTGCGGGCGATGGATGTGAGGCCGCACCTTTTCTCAAGGGACTCTATTTTACTTGCGTCGACTGCTTCCACCATAACGAAAATGAAACTTTCGATCTCTGCACCTCCTGCTACCGCAATGCTGCCTTTGTTCATGAACACACCAACTTTGTGGATAACCATGTGTTACTTCGTTCCAAGGCTGCTTGTTGTGAATCCACATCTGAGCCATGCTCTTCTGGGTCTGATTCTGAGTCCAATCAAGTTAGTCGATCTGGATCAAAGAGAGTGGAAGTTAAGCGATCTGGATCAAAGAGGAGGGCAGTATTTGGAGCAATCAATTCTGGAGTGAGCGTTGCACAGGCAGTAGGAAGTGTTGCAGCTGATGCTGCTTCATGCAGCATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

176

Amino Acids

19.11

Weight (kDa)

5.71

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 12 - 35 5.1e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 123
AccI GTMKAC 1 cut(s) 201
AciI CCGC 4 cut(s) 123, 146, 165, 256
AclWI GGATC 2 cut(s) 406, 436
AgsI TTSAA 2 cut(s) 49, 88
AluBI AGCT 4 cut(s) 9, 45, 55, 503
AluI AGCT 4 cut(s) 9, 45, 55, 503
Alw26I GTCTC 1 cut(s) 63
AlwI GGATC 2 cut(s) 406, 436
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 5 cut(s) 263, 323, 500, 509, 519
BbvI GCAGC 4 cut(s) 250, 310, 496, 512
BccI CCATC 1 cut(s) 146
BceAI ACGGC 1 cut(s) 88
BclI TGATCA 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 63
BisI GCNGC 6 cut(s) 165, 264, 324, 501, 510, 520
BlsI GCNGC 6 cut(s) 166, 265, 325, 502, 511, 521
BmsI GCATC 1 cut(s) 496
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpmI CTGGAG 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 161
BsaBI GATNNNNATC 2 cut(s) 397, 427
BsaJI CCNNGG 1 cut(s) 318
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 459, 489
Bse3DI GCAATG 1 cut(s) 265
Bse8I GATNNNNATC 2 cut(s) 397, 427
BseDI CCNNGG 1 cut(s) 318
BseGI GGATG 1 cut(s) 161
BseJI GATNNNNATC 2 cut(s) 397, 427
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 2 cut(s) 336, 363
BseRI GAGGAG 1 cut(s) 451
BseXI GCAGC 4 cut(s) 250, 310, 496, 512
BsgI GTGCAG 1 cut(s) 226
BshFI GGCC 1 cut(s) 164
BslFI GGGAC 1 cut(s) 195
BsmAI GTCTC 1 cut(s) 63
BsmBI CGTCTC 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 195
BsnI GGCC 1 cut(s) 164
Bsp143I GATC 6 cut(s) 30, 235, 392, 398, 422, 428
BspACI CCGC 4 cut(s) 123, 146, 165, 256
BspANI GGCC 1 cut(s) 164
BspCNI CTCAG 2 cut(s) 337, 364
BspHI TCATGA 2 cut(s) 33, 274
BspPI GGATC 2 cut(s) 406, 436
BspQI GCTCTTC 1 cut(s) 361
BsrBI CCGCTC 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 265
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 6 cut(s) 30, 235, 392, 398, 422, 428
BssT1I CCWWGG 1 cut(s) 318
Bst6I CTCTTC 2 cut(s) 32, 361
BstAPI GCANNNNNTGC 2 cut(s) 249, 506
BstC8I GCNNGC 1 cut(s) 148
BstDEI CTNAG 2 cut(s) 345, 372
BstF5I GGATG 1 cut(s) 161
BstKTI GATC 6 cut(s) 33, 238, 395, 401, 425, 431
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 6 cut(s) 30, 235, 392, 398, 422, 428
BstMWI GCNNNNNNNGC 4 cut(s) 204, 249, 506, 509
BstV1I GCAGC 4 cut(s) 250, 310, 496, 512
BstXI CCANNNNNNTGG 1 cut(s) 292
BsuRI GGCC 1 cut(s) 164
BtgZI GCGATG 1 cut(s) 165
BtsCI GGATG 1 cut(s) 161
Cac8I GCNNGC 1 cut(s) 148
CciI TCATGA 2 cut(s) 33, 274
CseI GACGC 1 cut(s) 187
CspCI CAANNNNNGTGG 2 cut(s) 390, 425
CviAII CATG 6 cut(s) 34, 275, 302, 351, 516, 526
CviJI RGCY 7 cut(s) 9, 45, 55, 164, 323, 349, 503
CviKI_1 RGCY 7 cut(s) 9, 45, 55, 164, 323, 349, 503
DdeI CTNAG 2 cut(s) 345, 372
DpnI GATC 6 cut(s) 32, 237, 394, 400, 424, 430
DpnII GATC 6 cut(s) 30, 235, 392, 398, 422, 428
Eam1104I CTCTTC 2 cut(s) 32, 361
EarI CTCTTC 2 cut(s) 32, 361
Eco130I CCWWGG 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
Esp3I CGTCTC 1 cut(s) 63
FaeI CATG 6 cut(s) 37, 278, 305, 354, 519, 529
FaiI YATR 7 cut(s) 35, 216, 276, 303, 352, 517, 527
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 1 cut(s) 195
FatI CATG 6 cut(s) 33, 274, 301, 350, 515, 525
FauI CCCGC 1 cut(s) 139
FbaI TGATCA 1 cut(s) 30
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 6 cut(s) 165, 264, 324, 501, 510, 520
FokI GGATG 1 cut(s) 168
Fsp4HI GCNGC 6 cut(s) 165, 264, 324, 501, 510, 520
GluI GCNGC 6 cut(s) 165, 264, 324, 501, 510, 520
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 1 cut(s) 164
HgaI GACGC 1 cut(s) 187
Hin1II CATG 6 cut(s) 37, 278, 305, 354, 519, 529
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 43
HinfI GANTC 5 cut(s) 23, 183, 335, 368, 374
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 3 cut(s) 346, 367, 373
Hpy188III TCNNGA 5 cut(s) 34, 275, 396, 426, 465
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 1 cut(s) 203
HpyCH4V TGCA 4 cut(s) 243, 479, 500, 519
HpyF10VI GCNNNNNNNGC 4 cut(s) 204, 249, 506, 509
HpyF3I CTNAG 2 cut(s) 345, 372
Hsp92II CATG 6 cut(s) 37, 278, 305, 354, 519, 529
Ksp22I TGATCA 1 cut(s) 30
Kzo9I GATC 6 cut(s) 30, 235, 392, 398, 422, 428
LguI GCTCTTC 1 cut(s) 361
LmnI GCTCC 2 cut(s) 52, 452
LpnPI CCDG 7 cut(s) 41, 262, 345, 381, 411, 450, 467
Lsp1109I GCAGC 4 cut(s) 250, 310, 496, 512
LweI GCATC 1 cut(s) 496
MaeIII GTNAC 1 cut(s) 306
MalI GATC 6 cut(s) 32, 237, 394, 400, 424, 430
MbiI CCGCTC 1 cut(s) 123
MboI GATC 6 cut(s) 30, 235, 392, 398, 422, 428
MboII GAAGA 2 cut(s) 49, 348
MluCI AATT 1 cut(s) 460
MlyI GAGTC 3 cut(s) 17, 177, 383
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 4 cut(s) 154, 256, 429, 432
MseI TTAA 1 cut(s) 417
MspA1I CMGCKG 1 cut(s) 503
MwoI GCNNNNNNNGC 4 cut(s) 204, 249, 506, 509
NdeII GATC 6 cut(s) 30, 235, 392, 398, 422, 428
NlaIII CATG 6 cut(s) 37, 278, 305, 354, 519, 529
PagI TCATGA 2 cut(s) 33, 274
PciSI GCTCTTC 1 cut(s) 361
PfeI GAWTC 2 cut(s) 335, 368
PkrI GCNGC 6 cut(s) 166, 265, 325, 502, 511, 521
PleI GAGTC 3 cut(s) 17, 177, 382
PpsI GAGTC 3 cut(s) 17, 177, 382
PvuII CAGCTG 1 cut(s) 503
SalI GTCGAC 1 cut(s) 200
SapI GCTCTTC 1 cut(s) 361
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 6 cut(s) 165, 264, 324, 501, 510, 520
Sau3AI GATC 6 cut(s) 30, 235, 392, 398, 422, 428
SchI GAGTC 3 cut(s) 17, 177, 383
SetI ASST 6 cut(s) 11, 47, 57, 172, 248, 505
SfaNI GCATC 1 cut(s) 496
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 1 cut(s) 460
SsiI CCGC 4 cut(s) 123, 146, 165, 256
StyI CCWWGG 1 cut(s) 318
TaqI TCGA 4 cut(s) 15, 201, 234, 391
TasI AATT 1 cut(s) 460
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 2 cut(s) 335, 368
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TseI GCWGC 5 cut(s) 263, 323, 500, 509, 519
TspDTI ATGAA 5 cut(s) 50, 240, 263, 291, 504
TspGWI ACGGA 2 cut(s) 16, 81
XmiI GTMKAC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.