RchiOBHm_Chr5g0024661

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000286
Physical Location & Seq
Reverse (-)
18744413 .. 18745003
591 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30440

Sequence Viewer

Length: 591 bp
ATGGAGGAATTACTCCAGGCAGCAGTAGCATACTACAACAATGGTTCGAATAATCTCCGGGAACTAGCATCCAACTTCTTCAAGAGCATGGACACCAACCGTGACCGTCGAGTAAGCCGGTCGGAGTTCGTCCGATTTCTACAGCAAAGCGGGTACAATATCAACCCCTACATCTTCAACGCGCTCGATCGAAACGGCGACGGTTACCTGGACTTTTGGGAAGTTCTCACACTGTACTACATCATCAAAACCAGGTTGTATCATTGTGCTGACTGCGGAGTACGCCTCTTGGGGCTGTATTTCACGTGTGTTGAGTGCTTTGACAGAGCCGGCGATACCTATGATCTGTGCTCCATCTGCTATTCGGCCAGGCAGTTTCGTCACAGCCACACTCTCTTCTTGGATAATCATATGCTGCTCATAGCAAAAAGAAGACAAGCTCGTGGTAGTCATAACCTGAATCAGGTATTGCTTATTCTTTTTCTTTTTCTTTTTTTAACGGAACAGGGCAGTGGCTTATCTACCGCTCCATTGAATAAAAGATCGAAGAAAAGAGTACAAGGGATGAGGACCCAACCAAAACCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

196

Amino Acids

22.83

Weight (kDa)

9.3

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 26 - 80 2.6e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 527
AccII CGCG 1 cut(s) 182
AciI CCGC 3 cut(s) 150, 276, 525
AcoI YGGCCR 1 cut(s) 366
AcvI CACGTG 1 cut(s) 306
AfaI GTAC 4 cut(s) 155, 236, 282, 558
AfiI CCNNNNNNNGG 1 cut(s) 463
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 3 cut(s) 82, 178, 535
AjnI CCWGG 4 cut(s) 15, 207, 251, 368
AluBI AGCT 1 cut(s) 440
AluI AGCT 1 cut(s) 440
Alw21I GWGCWC 1 cut(s) 353
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 2 cut(s) 20, 415
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 1 cut(s) 570
AsuC2I CCSGG 1 cut(s) 59
AsuII TTCGAA 1 cut(s) 47
AvaII GGWCC 1 cut(s) 570
BauI CACGAG 1 cut(s) 441
BbrPI CACGTG 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 439
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 2 cut(s) 32, 402
BccI CCATC 1 cut(s) 362
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 4 cut(s) 17, 209, 253, 370
BcnI CCSGG 1 cut(s) 59
BfaI CTAG 1 cut(s) 65
BfmI CTRYAG 1 cut(s) 140
BisI GCNGC 2 cut(s) 21, 416
BlsI GCNGC 2 cut(s) 22, 417
Bme1390I CCNGG 5 cut(s) 17, 59, 209, 253, 370
Bme18I GGWCC 1 cut(s) 570
BmgT120I GGNCC 1 cut(s) 570
BmiI GGNNCC 1 cut(s) 572
BmrFI CCNGG 5 cut(s) 17, 59, 209, 253, 370
BmsI GCATC 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 439
BplI GAGNNNNNCTC 2 cut(s) 270, 302
Bpu14I TTCGAA 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 59
BsaAI YACGTR 1 cut(s) 306
Bsc4I CCNNNNNNNGG 1 cut(s) 463
Bse118I RCCGGY 2 cut(s) 117, 329
BseBI CCWGG 4 cut(s) 17, 209, 253, 370
BseGI GGATG 2 cut(s) 68, 570
BseLI CCNNNNNNNGG 1 cut(s) 463
BseXI GCAGC 2 cut(s) 32, 402
Bsh1236I CGCG 1 cut(s) 182
Bsh1285I CGRYCG 2 cut(s) 122, 190
BshFI GGCC 1 cut(s) 368
BsiEI CGRYCG 2 cut(s) 122, 190
BsiHKAI GWGCWC 1 cut(s) 353
BsiSI CCGG 3 cut(s) 58, 118, 330
BslI CCNNNNNNNGG 1 cut(s) 463
BsnI GGCC 1 cut(s) 368
Bsp119I TTCGAA 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 3 cut(s) 187, 343, 542
BspACI CCGC 3 cut(s) 150, 276, 525
BspANI GGCC 1 cut(s) 368
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 1 cut(s) 572
BspT104I TTCGAA 1 cut(s) 47
BsrBI CCGCTC 1 cut(s) 527
BsrFI RCCGGY 2 cut(s) 117, 329
BssAI RCCGGY 2 cut(s) 117, 329
BssMI GATC 3 cut(s) 187, 343, 542
BssSI CACGAG 1 cut(s) 441
Bst2BI CACGAG 1 cut(s) 441
Bst2UI CCWGG 4 cut(s) 17, 209, 253, 370
Bst4CI ACNGT 4 cut(s) 101, 107, 203, 234
Bst6I CTCTTC 1 cut(s) 401
BstBAI YACGTR 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 331
BstEII GGTNACC 1 cut(s) 203
BstENI CCTNNNNNAGG 1 cut(s) 461
BstF5I GGATG 2 cut(s) 68, 570
BstFNI CGCG 1 cut(s) 182
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 3 cut(s) 190, 346, 545
BstMBI GATC 3 cut(s) 187, 343, 542
BstMCI CGRYCG 2 cut(s) 122, 190
BstMWI GCNNNNNNNGC 3 cut(s) 26, 282, 357
BstNI CCWGG 4 cut(s) 17, 209, 253, 370
BstPI GGTNACC 1 cut(s) 203
BstSCI CCNGG 5 cut(s) 15, 57, 207, 251, 368
BstSFI CTRYAG 1 cut(s) 140
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 2 cut(s) 32, 402
BstV2I GAAGAC 1 cut(s) 439
BsuRI GGCC 1 cut(s) 368
BtsCI GGATG 2 cut(s) 68, 570
BtsI GCAGTG 1 cut(s) 517
BtsIMutI CAGTG 2 cut(s) 230, 517
Cac8I GCNNGC 1 cut(s) 331
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 2 cut(s) 117, 329
Cfr13I GGNCC 1 cut(s) 570
CsiI ACCWGGT 1 cut(s) 251
Csp6I GTAC 4 cut(s) 154, 235, 281, 557
CviAII CATG 1 cut(s) 88
CviJI RGCY 7 cut(s) 117, 295, 329, 368, 387, 440, 516
CviKI_1 RGCY 7 cut(s) 117, 295, 329, 368, 387, 440, 516
CviQI GTAC 4 cut(s) 154, 235, 281, 557
DpnI GATC 3 cut(s) 189, 345, 544
DpnII GATC 3 cut(s) 187, 343, 542
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Eco47I GGWCC 1 cut(s) 570
Eco72I CACGTG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 203
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoO109I RGGNCCY 1 cut(s) 570
EcoO65I GGTNACC 1 cut(s) 203
EcoRII CCWGG 4 cut(s) 15, 207, 251, 368
FaeI CATG 1 cut(s) 91
FaiI YATR 7 cut(s) 31, 89, 342, 411, 413, 422, 453
FatI CATG 1 cut(s) 87
FauI CCCGC 1 cut(s) 143
FauNDI CATATG 1 cut(s) 411
Fnu4HI GCNGC 2 cut(s) 21, 416
FokI GGATG 2 cut(s) 55, 577
Fsp4HI GCNGC 2 cut(s) 21, 416
FspBI CTAG 1 cut(s) 65
GlaI GCGC 1 cut(s) 183
GluI GCNGC 2 cut(s) 21, 416
HaeIII GGCC 1 cut(s) 368
HapII CCGG 3 cut(s) 58, 118, 330
HhaI GCGC 1 cut(s) 184
Hin1II CATG 1 cut(s) 91
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HinfI GANTC 1 cut(s) 460
HpaII CCGG 3 cut(s) 58, 118, 330
Hpy188I TCNGA 2 cut(s) 124, 134
Hpy188III TCNNGA 1 cut(s) 82
Hpy99I CGWCG 2 cut(s) 111, 203
HpyCH4III ACNGT 4 cut(s) 101, 107, 203, 234
HpyCH4IV ACGT 1 cut(s) 305
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 282, 357
HpySE526I ACGT 1 cut(s) 305
Hsp92II CATG 1 cut(s) 91
HspAI GCGC 1 cut(s) 182
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Kzo9I GATC 3 cut(s) 187, 343, 542
LmnI GCTCC 2 cut(s) 356, 532
Lsp1109I GCAGC 2 cut(s) 32, 402
LweI GCATC 1 cut(s) 77
MabI ACCWGGT 1 cut(s) 251
MaeI CTAG 1 cut(s) 65
MaeII ACGT 1 cut(s) 305
MaeIII GTNAC 3 cut(s) 101, 203, 380
MalI GATC 3 cut(s) 189, 345, 544
MbiI CCGCTC 1 cut(s) 527
MboI GATC 3 cut(s) 187, 343, 542
MboII GAAGA 5 cut(s) 70, 166, 388, 444, 559
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 1 cut(s) 8
MmeI TCCRAC 2 cut(s) 96, 102
MnlI CCTC 2 cut(s) 296, 561
MroNI GCCGGC 1 cut(s) 329
MseI TTAA 1 cut(s) 497
MspI CCGG 3 cut(s) 58, 118, 330
MspR9I CCNGG 5 cut(s) 17, 59, 209, 253, 370
MvaI CCWGG 4 cut(s) 17, 209, 253, 370
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 3 cut(s) 26, 282, 357
NaeI GCCGGC 1 cut(s) 331
NciI CCSGG 1 cut(s) 59
NdeI CATATG 1 cut(s) 411
NdeII GATC 3 cut(s) 187, 343, 542
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 1 cut(s) 572
NmuCI GTSAC 2 cut(s) 101, 380
NspV TTCGAA 1 cut(s) 47
PdiI GCCGGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 460
PfoI TCCNGGA 1 cut(s) 57
PkrI GCNGC 2 cut(s) 22, 417
Ple19I CGATCG 1 cut(s) 190
PmaCI CACGTG 1 cut(s) 306
PmlI CACGTG 1 cut(s) 306
Ppu21I YACGTR 1 cut(s) 306
PpuMI RGGWCCY 1 cut(s) 570
Psp5II RGGWCCY 1 cut(s) 570
Psp6I CCWGG 4 cut(s) 15, 207, 251, 368
PspCI CACGTG 1 cut(s) 306
PspEI GGTNACC 1 cut(s) 203
PspGI CCWGG 4 cut(s) 15, 207, 251, 368
PspN4I GGNNCC 1 cut(s) 572
PspPI GGNCC 1 cut(s) 570
PspPPI RGGWCCY 1 cut(s) 570
PvuI CGATCG 1 cut(s) 190
RsaI GTAC 4 cut(s) 155, 236, 282, 558
RsaNI GTAC 4 cut(s) 154, 235, 281, 557
SaqAI TTAA 1 cut(s) 497
SatI GCNGC 2 cut(s) 21, 416
Sau3AI GATC 3 cut(s) 187, 343, 542
Sau96I GGNCC 1 cut(s) 570
ScrFI CCNGG 5 cut(s) 17, 59, 209, 253, 370
SduI GDGCHC 1 cut(s) 353
SetI ASST 8 cut(s) 210, 257, 308, 341, 442, 459, 468, 586
SexAI ACCWGGT 1 cut(s) 251
SfaNI GCATC 1 cut(s) 77
SfcI CTRYAG 1 cut(s) 140
SfuI TTCGAA 1 cut(s) 47
SinI GGWCC 1 cut(s) 570
Sse9I AATT 1 cut(s) 8
SsiI CCGC 3 cut(s) 150, 276, 525
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 5 cut(s) 15, 57, 207, 251, 368
TaaI ACNGT 4 cut(s) 101, 107, 203, 234
TaiI ACGT 1 cut(s) 308
TaqI TCGA 5 cut(s) 47, 109, 186, 190, 545
TasI AATT 1 cut(s) 8
TatI WGTACW 2 cut(s) 234, 556
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 1 cut(s) 497
Tru9I TTAA 1 cut(s) 497
TscAI CASTG 2 cut(s) 237, 517
TseFI GTSAC 2 cut(s) 101, 380
TseI GCWGC 2 cut(s) 20, 415
Tsp45I GTSAC 2 cut(s) 101, 380
TspGWI ACGGA 1 cut(s) 515
TspRI CASTG 2 cut(s) 237, 517
VpaK11BI GGWCC 1 cut(s) 570
XagI CCTNNNNNAGG 1 cut(s) 461
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.