Prupe.1G049800_v2.0.a1

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000286
Physical Location & Seq
Forward (+)
3493637 .. 3494077
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G049800.1

Sequence Viewer

Length: 441 bp
ATGGAGGAGCTGCGGCAGACGGTGCTTGCTTATTACAAGGATGCTCCACAACACATAAAGCGGTCCGTGGATGAGTGTTTCATAGAGATGGACGTGGATGGGAATGACCGAGTGAGCTGGCAAGAGTTCTTGGCATACATGGAGATGCATGAAGATTGCAAGCATCTGAGTACTTGCAGCTTCTTCAATGAGCTCAAGAAGGAAGAAAAGGAAGGGTTAGATTTCATGGACGTGGTGATTCTCGTCTACATAATTTACAGTGGAAAACCATTCTGCAATGGGCACTCTGGAAGCTTTATAAAGGGAACGTATTTCACTTGTGTCAAGTGCTTTGATGGCCATGAGCATGGCCAATGTAGTGTCCCAAACAAGACCTTCAACGTTTGCACTGTTTGTTACGTTGATGGCAAAATTTGTCCATGGCCTCGAATGGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5

Protein Analysis

147

Amino Acids

16.94

Weight (kDa)

5.3

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 299
AccI GTMKAC 1 cut(s) 246
AciI CCGC 2 cut(s) 13, 61
AclI AACGTT 1 cut(s) 381
AcoI YGGCCR 2 cut(s) 337, 349
AcsI RAATTY 1 cut(s) 411
AfaI GTAC 1 cut(s) 172
AgsI TTSAA 2 cut(s) 187, 379
AjiI CACGTC 2 cut(s) 94, 232
AluBI AGCT 5 cut(s) 10, 117, 180, 193, 294
AluI AGCT 5 cut(s) 10, 117, 180, 193, 294
Alw21I GWGCWC 1 cut(s) 195
AoxI GGCC 3 cut(s) 337, 349, 422
ApeKI GCWGC 2 cut(s) 10, 177
ApoI RAATTY 1 cut(s) 411
Asp700I GAANNNNTTC 1 cut(s) 433
AspS9I GGNCC 1 cut(s) 63
AsuHPI GGTGA 1 cut(s) 247
AvaII GGWCC 1 cut(s) 63
BaeGI GKGCMC 1 cut(s) 285
BalI TGGCCA 2 cut(s) 339, 351
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 1 cut(s) 189
BccI CCATC 4 cut(s) 82, 92, 329, 398
BisI GCNGC 3 cut(s) 11, 14, 178
BlsI GCNGC 3 cut(s) 12, 15, 179
BmcAI AGTACT 1 cut(s) 172
Bme18I GGWCC 1 cut(s) 63
BmgBI CACGTC 2 cut(s) 94, 232
BmgT120I GGNCC 1 cut(s) 63
BmsI GCATC 3 cut(s) 31, 135, 172
BpuEI CTTGAG 1 cut(s) 179
BsaJI CCNNGG 2 cut(s) 66, 419
Bse3DI GCAATG 1 cut(s) 283
BseDI CCNNGG 2 cut(s) 66, 419
BseGI GGATG 3 cut(s) 46, 76, 103
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 158
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 285
BseXI GCAGC 1 cut(s) 189
BshFI GGCC 3 cut(s) 339, 351, 424
BsiHKAI GWGCWC 1 cut(s) 195
BslFI GGGAC 1 cut(s) 347
BsmFI GGGAC 1 cut(s) 347
BsnI GGCC 3 cut(s) 339, 351, 424
Bsp1286I GDGCHC 2 cut(s) 195, 285
Bsp19I CCATGG 1 cut(s) 419
BspACI CCGC 2 cut(s) 13, 61
BspANI GGCC 3 cut(s) 339, 351, 424
BspCNI CTCAG 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 283
BssECI CCNNGG 2 cut(s) 66, 419
BssT1I CCWWGG 1 cut(s) 419
Bst4CI ACNGT 3 cut(s) 22, 260, 391
BstAPI GCANNNNNTGC 1 cut(s) 22
BstC8I GCNNGC 3 cut(s) 27, 119, 161
BstDEI CTNAG 1 cut(s) 167
BstDSI CCRYGG 2 cut(s) 66, 419
BstF5I GGATG 3 cut(s) 46, 76, 103
BstMWI GCNNNNNNNGC 2 cut(s) 22, 336
BstSLI GKGCMC 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 189
BstXI CCANNNNNNTGG 1 cut(s) 347
BsuRI GGCC 3 cut(s) 339, 351, 424
BtgI CCRYGG 2 cut(s) 66, 419
BtrI CACGTC 2 cut(s) 94, 232
BtsCI GGATG 3 cut(s) 46, 76, 103
BtsIMutI CAGTG 2 cut(s) 265, 387
Cac8I GCNNGC 3 cut(s) 27, 119, 161
Cfr13I GGNCC 1 cut(s) 63
CpoI CGGWCCG 1 cut(s) 63
Csp6I GTAC 1 cut(s) 171
CspI CGGWCCG 1 cut(s) 63
CviAII CATG 6 cut(s) 139, 149, 226, 341, 347, 420
CviJI RGCY 8 cut(s) 10, 117, 180, 193, 294, 339, 351, 424
CviKI_1 RGCY 8 cut(s) 10, 117, 180, 193, 294, 339, 351, 424
CviQI GTAC 1 cut(s) 171
DdeI CTNAG 1 cut(s) 167
EaeI YGGCCR 2 cut(s) 337, 349
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 419
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 193
EcoICRI GAGCTC 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 419
EcoT22I ATGCAT 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 6 cut(s) 142, 152, 229, 344, 350, 423
FaqI GGGAC 1 cut(s) 347
FatI CATG 6 cut(s) 138, 148, 225, 340, 346, 419
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 3 cut(s) 11, 14, 178
FokI GGATG 3 cut(s) 53, 83, 110
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 3 cut(s) 11, 14, 178
GluI GCNGC 3 cut(s) 11, 14, 178
HaeIII GGCC 3 cut(s) 339, 351, 424
Hin1II CATG 6 cut(s) 142, 152, 229, 344, 350, 423
HindIII AAGCTT 1 cut(s) 292
HinfI GANTC 1 cut(s) 238
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 1 cut(s) 168
Hpy188III TCNNGA 3 cut(s) 196, 288, 438
Hpy8I GTNNAC 1 cut(s) 247
HpyAV CCTTC 3 cut(s) 193, 206, 385
HpyCH4III ACNGT 3 cut(s) 22, 260, 391
HpyCH4IV ACGT 5 cut(s) 93, 231, 308, 381, 399
HpyCH4V TGCA 5 cut(s) 148, 159, 177, 276, 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 336
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 5 cut(s) 93, 231, 308, 381, 399
Hsp92II CATG 6 cut(s) 142, 152, 229, 344, 350, 423
LmnI GCTCC 2 cut(s) 7, 49
LpnPI CCDG 2 cut(s) 103, 273
Lsp1109I GCAGC 1 cut(s) 189
LweI GCATC 3 cut(s) 31, 135, 172
MaeII ACGT 5 cut(s) 93, 231, 308, 381, 399
MaeIII GTNAC 1 cut(s) 395
MboII GAAGA 3 cut(s) 164, 175, 215
MhlI GDGCHC 2 cut(s) 195, 285
MlsI TGGCCA 2 cut(s) 339, 351
MluCI AATT 2 cut(s) 252, 411
MluNI TGGCCA 2 cut(s) 339, 351
MnlI CCTC 1 cut(s) 435
Mox20I TGGCCA 2 cut(s) 339, 351
Mph1103I ATGCAT 1 cut(s) 150
MroXI GAANNNNTTC 1 cut(s) 433
MscI TGGCCA 2 cut(s) 339, 351
MslI CAYNNNNRTG 4 cut(s) 86, 143, 230, 345
Msp20I TGGCCA 2 cut(s) 339, 351
MwoI GCNNNNNNNGC 2 cut(s) 22, 336
NcoI CCATGG 1 cut(s) 419
NlaIII CATG 6 cut(s) 142, 152, 229, 344, 350, 423
NsiI ATGCAT 1 cut(s) 150
PdmI GAANNNNTTC 1 cut(s) 433
PfeI GAWTC 1 cut(s) 238
PkrI GCNGC 3 cut(s) 12, 15, 179
PsiI TTATAA 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 195
Psp1406I AACGTT 1 cut(s) 381
PspPI GGNCC 1 cut(s) 63
RsaI GTAC 1 cut(s) 172
RsaNI GTAC 1 cut(s) 171
RseI CAYNNNNRTG 4 cut(s) 86, 143, 230, 345
Rsr2I CGGWCCG 1 cut(s) 63
RsrII CGGWCCG 1 cut(s) 63
SacI GAGCTC 1 cut(s) 195
SatI GCNGC 3 cut(s) 11, 14, 178
Sau96I GGNCC 1 cut(s) 63
ScaI AGTACT 1 cut(s) 172
SduI GDGCHC 2 cut(s) 195, 285
SfaNI GCATC 3 cut(s) 31, 135, 172
SinI GGWCC 1 cut(s) 63
SmiMI CAYNNNNRTG 4 cut(s) 86, 143, 230, 345
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 2 cut(s) 252, 411
SsiI CCGC 2 cut(s) 13, 61
SstI GAGCTC 1 cut(s) 195
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 3 cut(s) 22, 260, 391
TaiI ACGT 5 cut(s) 96, 234, 311, 384, 402
TaqI TCGA 1 cut(s) 427
TaqII GACCGA 1 cut(s) 123
TasI AATT 2 cut(s) 252, 411
TatI WGTACW 1 cut(s) 170
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 1 cut(s) 238
TscAI CASTG 2 cut(s) 265, 394
TseI GCWGC 2 cut(s) 10, 177
TspDTI ATGAA 3 cut(s) 70, 165, 214
TspGWI ACGGA 1 cut(s) 55
TspRI CASTG 2 cut(s) 265, 394
VpaK11BI GGWCC 1 cut(s) 63
XapI RAATTY 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 246
XmnI GAANNNNTTC 1 cut(s) 433
ZrmI AGTACT 1 cut(s) 172
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.