Rorug03G0356400

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000286
Physical Location & Seq
Forward (+)
44224762 .. 44225123
362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0356400.1

Sequence Viewer

Length: 294 bp
ATGGATTCATTAATGGAAGGTGTAACCTACAAGGTTGCAACCATTACGCCCTTGGCTGCTGGGTTCCTTACATTTGTTGCTGTGGAGTGCTTTGTTGATCAATTGGTTGCTCCAATCAAACCCACAGTTGGGCAGTACTTGGCCAAGTACCTTGTTGCCTGCAGTGGACCTGTGGTCACCAAATACTTGAACATCTACGTTGCTTACATTCTCAGCTACAAGACCAACATCGAGAATCTGTTGAAACAAGTAAAGAAGCTCAAGGCCGCAAGAGACTGTTGGAAATTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

97

Amino Acids

10.81

Weight (kDa)

9.17

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 1 cut(s) 284
AfaI GTAC 2 cut(s) 137, 149
AfiI CCNNNNNNNGG 2 cut(s) 128, 129
AgsI TTSAA 2 cut(s) 190, 244
AhdI GACNNNNNGTC 1 cut(s) 173
AluBI AGCT 2 cut(s) 216, 259
AluI AGCT 2 cut(s) 216, 259
Alw26I GTCTC 1 cut(s) 267
AoxI GGCC 2 cut(s) 141, 264
ApeKI GCWGC 1 cut(s) 56
ApoI RAATTY 1 cut(s) 284
AseI ATTAAT 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 169
AvaII GGWCC 1 cut(s) 167
BalI TGGCCA 1 cut(s) 143
BbvI GCAGC 1 cut(s) 43
BclI TGATCA 1 cut(s) 97
BcoDI GTCTC 1 cut(s) 267
BfmI CTRYAG 1 cut(s) 160
BisI GCNGC 2 cut(s) 57, 267
BlsI GCNGC 2 cut(s) 58, 268
BmcAI AGTACT 1 cut(s) 137
Bme18I GGWCC 1 cut(s) 167
BmeRI GACNNNNNGTC 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 129
BseDI CCNNGG 1 cut(s) 51
BseLI CCNNNNNNNGG 2 cut(s) 128, 129
BseMII CTCAG 1 cut(s) 226
BseXI GCAGC 1 cut(s) 43
BseYI CCCAGC 1 cut(s) 59
BshFI GGCC 2 cut(s) 143, 266
BslI CCNNNNNNNGG 2 cut(s) 128, 129
BsmAI GTCTC 1 cut(s) 267
BsnI GGCC 2 cut(s) 143, 266
Bsp143I GATC 1 cut(s) 97
BspACI CCGC 1 cut(s) 267
BspANI GGCC 2 cut(s) 143, 266
BspCNI CTCAG 1 cut(s) 225
BspLI GGNNCC 1 cut(s) 65
BspMAI CTGCAG 1 cut(s) 164
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 1 cut(s) 97
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 2 cut(s) 127, 278
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 212
BstEII GGTNACC 1 cut(s) 175
BstKTI GATC 1 cut(s) 100
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 1 cut(s) 97
BstPI GGTNACC 1 cut(s) 175
BstSFI CTRYAG 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 43
BsuRI GGCC 2 cut(s) 143, 266
BtsI GCAGTG 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 167
Csp6I GTAC 2 cut(s) 136, 148
CviJI RGCY 5 cut(s) 56, 143, 216, 259, 266
CviKI_1 RGCY 5 cut(s) 56, 143, 216, 259, 266
CviQI GTAC 2 cut(s) 136, 148
DdeI CTNAG 1 cut(s) 212
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
DriI GACNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 141
Eam1105I GACNNNNNGTC 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 167
Eco91I GGTNACC 1 cut(s) 175
EcoO65I GGTNACC 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 51
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 2 cut(s) 57, 267
Fsp4HI GCNGC 2 cut(s) 57, 267
GluI GCNGC 2 cut(s) 57, 267
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 2 cut(s) 143, 266
HinfI GANTC 2 cut(s) 5, 235
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 167
Hpy188III TCNNGA 1 cut(s) 232
Hpy8I GTNNAC 1 cut(s) 167
HpyAV CCTTC 1 cut(s) 11
HpyCH4III ACNGT 2 cut(s) 127, 278
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 2 cut(s) 38, 162
HpyF3I CTNAG 1 cut(s) 212
HpySE526I ACGT 1 cut(s) 198
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 1 cut(s) 97
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 3 cut(s) 45, 172, 183
Lsp1109I GCAGC 1 cut(s) 43
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 2 cut(s) 22, 175
MalI GATC 1 cut(s) 99
MboI GATC 1 cut(s) 97
MfeI CAATTG 1 cut(s) 101
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 3 cut(s) 101, 284, 289
MluNI TGGCCA 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 260
Mox20I TGGCCA 1 cut(s) 143
MscI TGGCCA 1 cut(s) 143
MseI TTAA 3 cut(s) 11, 288, 292
Msp20I TGGCCA 1 cut(s) 143
MunI CAATTG 1 cut(s) 101
NdeII GATC 1 cut(s) 97
NlaIV GGNNCC 1 cut(s) 65
NmuCI GTSAC 1 cut(s) 175
PacI TTAATTAA 1 cut(s) 292
PfeI GAWTC 2 cut(s) 5, 235
PkrI GCNGC 2 cut(s) 58, 268
PshBI ATTAAT 1 cut(s) 11
PspEI GGTNACC 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 59
PspN4I GGNNCC 1 cut(s) 65
PspPI GGNCC 1 cut(s) 167
PstI CTGCAG 1 cut(s) 164
RsaI GTAC 2 cut(s) 137, 149
RsaNI GTAC 2 cut(s) 136, 148
SaqAI TTAA 3 cut(s) 11, 288, 292
SatI GCNGC 2 cut(s) 57, 267
Sau3AI GATC 1 cut(s) 97
Sau96I GGNCC 1 cut(s) 167
ScaI AGTACT 1 cut(s) 137
SetI ASST 8 cut(s) 22, 29, 36, 153, 172, 201, 218, 261
SfcI CTRYAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 3 cut(s) 101, 284, 289
SsiI CCGC 1 cut(s) 267
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 2 cut(s) 127, 278
TaiI ACGT 1 cut(s) 201
TaqI TCGA 1 cut(s) 231
TasI AATT 3 cut(s) 101, 284, 289
TatI WGTACW 1 cut(s) 135
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 2 cut(s) 5, 235
Tru1I TTAA 3 cut(s) 11, 288, 292
Tru9I TTAA 3 cut(s) 11, 288, 292
TscAI CASTG 1 cut(s) 169
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 1 cut(s) 56
Tsp45I GTSAC 1 cut(s) 175
TspRI CASTG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 167
VspI ATTAAT 1 cut(s) 11
XapI RAATTY 1 cut(s) 284
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
ZrmI AGTACT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.