Rh5BG173500

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000286
Physical Location & Seq
Reverse (-)
18126132 .. 18127209
1078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG173500.1

Sequence Viewer

Length: 591 bp
ATGGACGAGTTGCATCAGGCAGCTTCAGCATATTACAACAACGGGTCGCCTGAGATTCAGCAACTAGCATGGAGCTTCTTCCAATCCATGGACACAAACTGCGACGGCCAAGTTGACTGGAACGAGTTCTCCGCATTTCTCTACCAAAGCGGCAACCAATGGATCAGCCCCAGCTTCTTCAACGATCTGGACCGGAACCACGACGGTGCTCTCGATTTCATGGAAGTTCTCACGTTTTACTATATCATCAAGACTAGGAGCTTCTGGTGCAGCGTCTGCGGGATTTGTCTCACGGGGCTCTACTTCACTTGTGTTGAGTGCTTTGACAACGTGAACTCAAACACTTACGATCTTTGTCCCAGATGCTATGAGTCGAAGCCGGAAAGGCAAAGGTATAACCAGCAGAGTGATCACCATCATGACTACTTTATGGATAACCATATGTTGCTCAGATCAAAGAGAGGAGGATATGGTTCTGCAAACCTGGCCATAGATTCACCTGGAGGTGGACCAGGACGGACCAAAATGCGACAAACGATGAAAGCATTGGATACGGCAGTGAATGTGGCAACTTTGTGTAGCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8 ZZ

Protein Analysis

196

Amino Acids

22.48

Weight (kDa)

5.07

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 21 - 81 1.4e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 88
AciI CCGC 3 cut(s) 132, 150, 279
AclWI GGATC 1 cut(s) 170
AcoI YGGCCR 2 cut(s) 106, 486
AcuI CTGAAG 1 cut(s) 9
AfiI CCNNNNNNNGG 2 cut(s) 88, 506
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 3 cut(s) 483, 499, 511
AleI CACNNNNGTG 1 cut(s) 204
AloI GAACNNNNNNTCC 2 cut(s) 113, 145
AluBI AGCT 4 cut(s) 23, 75, 174, 261
AluI AGCT 4 cut(s) 23, 75, 174, 261
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 1 cut(s) 170
AlwNI CAGNNNCTG 1 cut(s) 276
AoxI GGCC 2 cut(s) 106, 486
ApeKI GCWGC 2 cut(s) 20, 270
Asp700I GAANNNNTTC 1 cut(s) 125
AspS9I GGNCC 3 cut(s) 190, 509, 519
AsuHPI GGTGA 2 cut(s) 404, 489
AvaII GGWCC 3 cut(s) 190, 509, 519
BaeI ACNNNNGTAYC 2 cut(s) 543, 576
BalI TGGCCA 1 cut(s) 488
BanII GRGCYC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 2 cut(s) 32, 282
BccI CCATC 1 cut(s) 423
BceAI ACGGC 2 cut(s) 121, 570
BciT130I CCWGG 3 cut(s) 485, 501, 513
BciVI GTATCC 1 cut(s) 544
BclI TGATCA 1 cut(s) 409
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 2 cut(s) 65, 255
BfuI GTATCC 1 cut(s) 544
BglI GCCNNNNNGGC 1 cut(s) 385
BisI GCNGC 3 cut(s) 21, 151, 271
BlsI GCNGC 3 cut(s) 22, 152, 272
Bme1390I CCNGG 3 cut(s) 485, 501, 513
Bme18I GGWCC 3 cut(s) 190, 509, 519
BmgT120I GGNCC 3 cut(s) 190, 509, 519
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 3 cut(s) 485, 501, 513
BmsI GCATC 2 cut(s) 22, 353
BpmI CTGGAG 1 cut(s) 522
BsaBI GATNNNNATC 1 cut(s) 414
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 506
Bse1I ACTGG 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 3 cut(s) 485, 501, 513
BseDI CCNNGG 1 cut(s) 87
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 88, 506
BseMII CTCAG 2 cut(s) 42, 463
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 477
BseXI GCAGC 2 cut(s) 32, 282
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 289
BshFI GGCC 2 cut(s) 108, 488
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 2 cut(s) 193, 380
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 2 cut(s) 88, 506
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 342
BsnI GGCC 2 cut(s) 108, 488
Bsp1286I GDGCHC 2 cut(s) 211, 300
Bsp143I GATC 5 cut(s) 162, 184, 349, 409, 452
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 3 cut(s) 132, 150, 279
BspANI GGCC 2 cut(s) 108, 488
BspCNI CTCAG 2 cut(s) 43, 462
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 1 cut(s) 197
BspPI GGATC 1 cut(s) 170
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 5 cut(s) 162, 184, 349, 409, 452
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 3 cut(s) 485, 501, 513
Bst4CI ACNGT 1 cut(s) 206
BstAPI GCANNNNNTGC 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 51, 449
BstDSI CCRYGG 1 cut(s) 87
BstKTI GATC 5 cut(s) 165, 187, 352, 412, 455
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 5 cut(s) 162, 184, 349, 409, 452
BstMWI GCNNNNNNNGC 5 cut(s) 26, 267, 276, 385, 485
BstNI CCWGG 3 cut(s) 485, 501, 513
BstSCI CCNGG 3 cut(s) 483, 499, 511
BstV1I GCAGC 2 cut(s) 32, 282
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 2 cut(s) 108, 488
BtgI CCRYGG 1 cut(s) 87
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 1 cut(s) 564
CaiI CAGNNNCTG 1 cut(s) 276
CciI TCATGA 1 cut(s) 418
Cfr13I GGNCC 3 cut(s) 190, 509, 519
CseI GACGC 1 cut(s) 262
CviAII CATG 4 cut(s) 69, 88, 220, 419
CviJI RGCY 9 cut(s) 23, 75, 108, 168, 174, 261, 298, 379, 488
CviKI_1 RGCY 9 cut(s) 23, 75, 108, 168, 174, 261, 298, 379, 488
DdeI CTNAG 2 cut(s) 51, 449
DpnI GATC 5 cut(s) 164, 186, 351, 411, 454
DpnII GATC 5 cut(s) 162, 184, 349, 409, 452
EaeI YGGCCR 2 cut(s) 106, 486
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 3 cut(s) 190, 509, 519
Eco57I CTGAAG 1 cut(s) 9
EcoRII CCWGG 3 cut(s) 483, 499, 511
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 4 cut(s) 72, 91, 223, 422
FaqI GGGAC 1 cut(s) 342
FatI CATG 4 cut(s) 68, 87, 219, 418
FauI CCCGC 1 cut(s) 272
FauNDI CATATG 1 cut(s) 441
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 3 cut(s) 21, 151, 271
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 3 cut(s) 21, 151, 271
FspBI CTAG 2 cut(s) 65, 255
GluI GCNGC 3 cut(s) 21, 151, 271
GsaI CCCAGC 1 cut(s) 174
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 2 cut(s) 108, 488
HapII CCGG 2 cut(s) 193, 380
HgaI GACGC 1 cut(s) 262
Hin1II CATG 4 cut(s) 72, 91, 223, 422
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 3 cut(s) 55, 371, 494
HpaII CCGG 2 cut(s) 193, 380
HphI GGTGA 2 cut(s) 404, 489
Hpy166II GTNNAC 3 cut(s) 115, 334, 509
Hpy188I TCNGA 1 cut(s) 452
Hpy188III TCNNGA 4 cut(s) 188, 212, 250, 419
Hpy8I GTNNAC 3 cut(s) 115, 334, 509
Hpy99I CGWCG 2 cut(s) 107, 206
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 2 cut(s) 233, 330
HpyCH4V TGCA 3 cut(s) 13, 270, 479
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 267, 276, 385, 485
HpyF3I CTNAG 2 cut(s) 51, 449
HpySE526I ACGT 2 cut(s) 233, 330
Hsp92II CATG 4 cut(s) 72, 91, 223, 422
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 5 cut(s) 162, 184, 349, 409, 452
LmnI GCTCC 2 cut(s) 72, 258
Lsp1109I GCAGC 2 cut(s) 32, 282
LweI GCATC 2 cut(s) 22, 353
MaeI CTAG 2 cut(s) 65, 255
MaeII ACGT 2 cut(s) 233, 330
MalI GATC 5 cut(s) 164, 186, 351, 411, 454
MboI GATC 5 cut(s) 162, 184, 349, 409, 452
MboII GAAGA 2 cut(s) 70, 169
MhlI GDGCHC 2 cut(s) 211, 300
MlsI TGGCCA 1 cut(s) 488
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 1 cut(s) 380
MnlI CCTC 3 cut(s) 455, 458, 497
Mox20I TGGCCA 1 cut(s) 488
MroXI GAANNNNTTC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 488
MslI CAYNNNNRTG 2 cut(s) 204, 417
Msp20I TGGCCA 1 cut(s) 488
MspI CCGG 2 cut(s) 193, 380
MspR9I CCNGG 3 cut(s) 485, 501, 513
MvaI CCWGG 3 cut(s) 485, 501, 513
MwoI GCNNNNNNNGC 5 cut(s) 26, 267, 276, 385, 485
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 441
NdeII GATC 5 cut(s) 162, 184, 349, 409, 452
NlaIII CATG 4 cut(s) 72, 91, 223, 422
NlaIV GGNNCC 1 cut(s) 197
OliI CACNNNNGTG 1 cut(s) 204
PagI TCATGA 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 55, 494
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 3 cut(s) 22, 152, 272
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp6I CCWGG 3 cut(s) 483, 499, 511
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 3 cut(s) 483, 499, 511
PspN4I GGNNCC 1 cut(s) 197
PspPI GGNCC 3 cut(s) 190, 509, 519
PstNI CAGNNNCTG 1 cut(s) 276
RseI CAYNNNNRTG 2 cut(s) 204, 417
SatI GCNGC 3 cut(s) 21, 151, 271
Sau3AI GATC 5 cut(s) 162, 184, 349, 409, 452
Sau96I GGNCC 3 cut(s) 190, 509, 519
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 3 cut(s) 485, 501, 513
SduI GDGCHC 2 cut(s) 211, 300
SfaNI GCATC 2 cut(s) 22, 353
SinI GGWCC 3 cut(s) 190, 509, 519
SmiMI CAYNNNNRTG 2 cut(s) 204, 417
SsiI CCGC 3 cut(s) 132, 150, 279
SspMI CTAG 2 cut(s) 65, 255
StyD4I CCNGG 3 cut(s) 483, 499, 511
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 2 cut(s) 236, 333
TaqI TCGA 2 cut(s) 213, 374
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 55, 494
TscAI CASTG 1 cut(s) 564
TseI GCWGC 2 cut(s) 20, 270
TspDTI ATGAA 2 cut(s) 208, 554
TspGWI ACGGA 1 cut(s) 532
TspRI CASTG 1 cut(s) 564
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 3 cut(s) 190, 509, 519
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 2 cut(s) 65, 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.