FvH4_4g04860

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000286
Physical Location & Seq
Forward (+)
4226429 .. 4228600
2172 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04860.t1

Sequence Viewer

Length: 759 bp
ATGGACATGCAAGAAATACGCAACGCTGCTTTAGCTTATTACCAGAACTTATCTGATGAGCTAAAGCAGAAGGCAACTGAAAGATTTAAAGAAATGGATTCGAATGGTAATGGCACAATAAGCATCAATGAGTTCCGGCGGAGTTTGGGTAGCTCCGAGATCAAGCGCTGCTTCCAGTTGCTGGACAAAACCGGAGACGGCAAGTTAGATATTAACGAGTTCATCACTCTTTACTACCTTGTGGAGAGTGGGAGAGGGGTTTTCTGTAGCGGCAATGGATGTAAGGCCTCCCCTTTTCTCAACGGACTGTATTTTACTTGCGTTGAGTGCTTCCACAACAACGAAGCCGCAACGTTTGATCTCTGCACCTCCTGCTACCGCGATGGAGAGTATGTTCATAACCATGCTAGCTTTTTGGATAACTACGTCTTCCTGCGTTCCAAGGCTGCTTGTGAATCTACCACACCAACTGAAGATGATCATCATGAGGTAAACGCTGCAGAATCTGCTGAACCAATTAGTCGAGGACTTGTAAAAGTTTCCGATCTATTTAGTCGATTTGGGTCGTCTGAATCTCCAAGCTCTCCTAAGATCGATGGTGGGTCTTATGAGTCTGGTTCTGAGTATGACGCTGAATCCGATCAAGTTAGTCGATCTGGGTCTAAAAGAAGGGCAGTATTTGAAGCAATAAAATCACTGAATTCCCAAGTGAGCATTGGAGATGCTGCTGCAGATGAGTCCAGTTGCAGCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

253

Amino Acids

27.76

Weight (kDa)

4.81

Isoelectric Point (pI)

53.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 23 - 79 2.1e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 181
AccII CGCG 1 cut(s) 381
AciI CCGC 4 cut(s) 139, 270, 348, 379
AclI AACGTT 1 cut(s) 353
AcsI RAATTY 1 cut(s) 700
AcuI CTGAAG 1 cut(s) 492
AfeI AGCGCT 1 cut(s) 167
AfiI CCNNNNNNNGG 1 cut(s) 181
AgsI TTSAA 1 cut(s) 683
AloI GAACNNNNNNTCC 2 cut(s) 378, 410
AluBI AGCT 5 cut(s) 35, 61, 153, 411, 582
AluI AGCT 5 cut(s) 35, 61, 153, 411, 582
Alw26I GTCTC 1 cut(s) 189
AlwNI CAGNNNCTG 2 cut(s) 181, 506
Aor51HI AGCGCT 1 cut(s) 167
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 7 cut(s) 26, 168, 446, 497, 725, 728, 747
ApoI RAATTY 1 cut(s) 700
AspLEI GCGC 1 cut(s) 168
AsuII TTCGAA 1 cut(s) 101
AsuNHI GCTAGC 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 421
BbvI GCAGC 6 cut(s) 13, 155, 433, 484, 712, 715
BccI CCATC 2 cut(s) 377, 590
BceAI ACGGC 1 cut(s) 214
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 3 cut(s) 265, 498, 729
BfoI RGCGCY 1 cut(s) 169
BisI GCNGC 9 cut(s) 27, 169, 271, 348, 447, 498, 726, 729, 748
BlsI GCNGC 9 cut(s) 28, 170, 272, 349, 448, 499, 727, 730, 749
BmsI GCATC 2 cut(s) 132, 712
BmtI GCTAGC 1 cut(s) 411
BpiI GAAGAC 1 cut(s) 421
Bpu14I TTCGAA 1 cut(s) 101
Bsa29I ATCGAT 1 cut(s) 594
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 1 cut(s) 441
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 4 cut(s) 133, 163, 378, 408
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse1I ACTGG 2 cut(s) 175, 741
Bse3DI GCAATG 1 cut(s) 280
Bse8I GATNNNNATC 1 cut(s) 480
BseCI ATCGAT 1 cut(s) 594
BseDI CCNNGG 1 cut(s) 441
BseGI GGATG 1 cut(s) 284
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 1 cut(s) 612
BseNI ACTGG 2 cut(s) 175, 741
BseXI GCAGC 6 cut(s) 13, 155, 433, 484, 712, 715
BsgI GTGCAG 1 cut(s) 349
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 1 cut(s) 287
BshVI ATCGAT 1 cut(s) 594
BsiSI CCGG 2 cut(s) 136, 192
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 1 cut(s) 189
BsmBI CGTCTC 1 cut(s) 189
BsnI GGCC 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 101
Bsp143I GATC 7 cut(s) 159, 358, 478, 544, 591, 640, 653
BspACI CCGC 4 cut(s) 139, 270, 348, 379
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 1 cut(s) 613
BspDI ATCGAT 1 cut(s) 594
BspFNI CGCG 1 cut(s) 381
BspHI TCATGA 1 cut(s) 484
BspMAI CTGCAG 2 cut(s) 502, 733
BspOI GCTAGC 1 cut(s) 411
BspT104I TTCGAA 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 280
BsrI ACTGG 2 cut(s) 175, 741
BssECI CCNNGG 1 cut(s) 441
BssMI GATC 7 cut(s) 159, 358, 478, 544, 591, 640, 653
BssT1I CCWWGG 1 cut(s) 441
Bst4CI ACNGT 1 cut(s) 309
BstAPI GCANNNNNTGC 2 cut(s) 372, 506
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 2 cut(s) 588, 621
BstF5I GGATG 1 cut(s) 284
BstFNI CGCG 1 cut(s) 381
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 7 cut(s) 162, 361, 481, 547, 594, 643, 656
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 7 cut(s) 159, 358, 478, 544, 591, 640, 653
BstMWI GCNNNNNNNGC 5 cut(s) 32, 120, 327, 372, 506
BstNSI RCATGY 1 cut(s) 10
BstSFI CTRYAG 3 cut(s) 265, 498, 729
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 6 cut(s) 13, 155, 433, 484, 712, 715
BstV2I GAAGAC 1 cut(s) 421
Bsu15I ATCGAT 1 cut(s) 594
BsuRI GGCC 1 cut(s) 287
BsuTUI ATCGAT 1 cut(s) 594
BtgZI GCGATG 1 cut(s) 396
BtsCI GGATG 1 cut(s) 284
BtsIMutI CAGTG 1 cut(s) 695
Cac8I GCNNGC 1 cut(s) 409
CaiI CAGNNNCTG 2 cut(s) 181, 506
CciI TCATGA 1 cut(s) 484
CfoI GCGC 1 cut(s) 168
ClaI ATCGAT 1 cut(s) 594
CseI GACGC 1 cut(s) 638
CviAII CATG 3 cut(s) 7, 404, 485
CviJI RGCY 8 cut(s) 35, 61, 153, 287, 347, 411, 446, 582
CviKI_1 RGCY 8 cut(s) 35, 61, 153, 287, 347, 411, 446, 582
DdeI CTNAG 2 cut(s) 588, 621
DpnI GATC 7 cut(s) 161, 360, 480, 546, 593, 642, 655
DpnII GATC 7 cut(s) 159, 358, 478, 544, 591, 640, 653
DraI TTTAAA 1 cut(s) 88
EciI GGCGGA 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 441
Eco147I AGGCCT 1 cut(s) 287
Eco47III AGCGCT 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 492
EcoRI GAATTC 1 cut(s) 700
EcoT14I CCWWGG 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 441
Esp3I CGTCTC 1 cut(s) 189
FaeI CATG 3 cut(s) 10, 407, 488
FaiI YATR 8 cut(s) 8, 393, 399, 405, 486, 609, 627, 755
FalI AAGNNNNNCTT 2 cut(s) 155, 187
FatI CATG 3 cut(s) 6, 403, 484
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 9 cut(s) 27, 169, 271, 348, 447, 498, 726, 729, 748
FokI GGATG 1 cut(s) 291
Fsp4HI GCNGC 9 cut(s) 27, 169, 271, 348, 447, 498, 726, 729, 748
FspBI CTAG 1 cut(s) 408
GlaI GCGC 1 cut(s) 167
GluI GCNGC 9 cut(s) 27, 169, 271, 348, 447, 498, 726, 729, 748
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 1 cut(s) 287
HapII CCGG 2 cut(s) 136, 192
HgaI GACGC 1 cut(s) 638
HhaI GCGC 1 cut(s) 168
Hin1II CATG 3 cut(s) 10, 407, 488
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HinfI GANTC 7 cut(s) 98, 455, 503, 572, 611, 635, 737
HpaII CCGG 2 cut(s) 136, 192
Hpy166II GTNNAC 1 cut(s) 493
Hpy188I TCNGA 6 cut(s) 55, 157, 544, 571, 622, 640
Hpy188III TCNNGA 1 cut(s) 485
Hpy8I GTNNAC 1 cut(s) 493
HpyAV CCTTC 2 cut(s) 64, 663
HpyCH4III ACNGT 1 cut(s) 309
HpyCH4IV ACGT 2 cut(s) 353, 426
HpyCH4V TGCA 5 cut(s) 10, 366, 500, 731, 747
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 120, 327, 372, 506
HpyF3I CTNAG 2 cut(s) 588, 621
HpySE526I ACGT 2 cut(s) 353, 426
Hsp92II CATG 3 cut(s) 10, 407, 488
HspAI GCGC 1 cut(s) 166
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 7 cut(s) 159, 358, 478, 544, 591, 640, 653
LmnI GCTCC 1 cut(s) 158
Lsp1109I GCAGC 6 cut(s) 13, 155, 433, 484, 712, 715
LweI GCATC 2 cut(s) 132, 712
MaeI CTAG 1 cut(s) 408
MaeII ACGT 2 cut(s) 353, 426
MalI GATC 7 cut(s) 161, 360, 480, 546, 593, 642, 655
MboI GATC 7 cut(s) 159, 358, 478, 544, 591, 640, 653
MboII GAAGA 2 cut(s) 421, 485
MluCI AATT 2 cut(s) 516, 700
MlyI GAGTC 2 cut(s) 620, 746
MnlI CCTC 5 cut(s) 248, 298, 379, 481, 518
MseI TTAA 2 cut(s) 87, 213
MslI CAYNNNNRTG 1 cut(s) 402
MspI CCGG 2 cut(s) 136, 192
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 5 cut(s) 32, 120, 327, 372, 506
NdeII GATC 7 cut(s) 159, 358, 478, 544, 591, 640, 653
NheI GCTAGC 1 cut(s) 407
NlaIII CATG 3 cut(s) 10, 407, 488
NspI RCATGY 1 cut(s) 10
NspV TTCGAA 1 cut(s) 101
PagI TCATGA 1 cut(s) 484
PceI AGGCCT 1 cut(s) 287
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PfeI GAWTC 5 cut(s) 98, 455, 503, 572, 635
PflMI CCANNNNNTGG 1 cut(s) 181
PkrI GCNGC 9 cut(s) 28, 170, 272, 349, 448, 499, 727, 730, 749
PleI GAGTC 2 cut(s) 619, 745
PpsI GAGTC 2 cut(s) 619, 745
Psp1406I AACGTT 1 cut(s) 353
PstI CTGCAG 2 cut(s) 502, 733
PstNI CAGNNNCTG 2 cut(s) 181, 506
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 2 cut(s) 87, 213
SatI GCNGC 9 cut(s) 27, 169, 271, 348, 447, 498, 726, 729, 748
Sau3AI GATC 7 cut(s) 159, 358, 478, 544, 591, 640, 653
SchI GAGTC 2 cut(s) 620, 746
SfaNI GCATC 2 cut(s) 132, 712
SfcI CTRYAG 3 cut(s) 265, 498, 729
SfuI TTCGAA 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 402
Sse9I AATT 2 cut(s) 516, 700
SseBI AGGCCT 1 cut(s) 287
SsiI CCGC 4 cut(s) 139, 270, 348, 379
SspMI CTAG 1 cut(s) 408
StuI AGGCCT 1 cut(s) 287
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 1 cut(s) 309
TaiI ACGT 2 cut(s) 356, 429
TaqI TCGA 5 cut(s) 101, 523, 556, 594, 652
TasI AATT 2 cut(s) 516, 700
TauI GCSGC 2 cut(s) 273, 350
TfiI GAWTC 5 cut(s) 98, 455, 503, 572, 635
Tru1I TTAA 2 cut(s) 87, 213
Tru9I TTAA 2 cut(s) 87, 213
TscAI CASTG 1 cut(s) 702
TseI GCWGC 7 cut(s) 26, 168, 446, 497, 725, 728, 747
TspDTI ATGAA 2 cut(s) 211, 386
TspGWI ACGGA 1 cut(s) 318
TspRI CASTG 1 cut(s) 702
Van91I CCANNNNNTGG 1 cut(s) 181
XapI RAATTY 1 cut(s) 700
XceI RCATGY 1 cut(s) 10
XcmI CCANNNNNNNNNTGG 1 cut(s) 713
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.