RLG00000009621

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000286
Physical Location & Seq
Forward (+)
55498825 .. 55501488
2664 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009621

Sequence Viewer

Length: 648 bp
ATGGGAGACATCAAAGAAATCCGTGATGCTGCTTTAGCTTATTACCATAATTTGTCTGAAAAGCAAAAGCATGAGGTGTATACCTTTTTTCAAGAAATAGATACAGATGGTGATGGAACAATAACCATGGAGGAGTTCGAGCAGCTGTTTGAGGGTTGTTACGGCGACCATGTCAACCGTACCTTCCAGTACCTAGACAAAAATGGAGATGGCAGTTTGGATTTTGAGGAATCTGTCACTCTTTACTACTCTCTTATGACTCAGAAGATTATTGGTTGCGATGGATGCAAAACCCTTTTTCTAAAGGGTCTGTATTTCACTTGCGTGGAGTGCTTCGAGCATAACCAAACTACAACTTTTGATCTTTGCACCTCCTGCTACCGCAGCCGTAACTACGTTCACCAACACTGCAACTTTTTGGACAACTACGCCTTACTGCGTGCCAAGGCTGCTTATAACAATTCTGGATCGACAAACCAGCCAACAAGCTCGGCAAAGTCGATTGATGGATATGATCTTGAGAACTATGAAGTTCTCCAATCGAGATCTAAAAAATTAGCAGCTCTGGAAGCCTTCACTACCGGTGTTGAGATCGGAAATGCTGTGGGAAATATTGCTGTTGCCGCTAGTTCATGCATCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

216

Amino Acids

24.22

Weight (kDa)

4.78

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 14 - 50 2.5e-07 EF-hand domain pair
EF-hand_1 PF00036 26 - 51 1.8e-06 EF hand domain
EF-hand_6 PF13405 28 - 51 5.6e-06 EF-hand domain
EF-hand_5 PF13202 29 - 49 1.5e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 456
AccI GTMKAC 1 cut(s) 80
AciI CCGC 2 cut(s) 382, 624
AclWI GGATC 1 cut(s) 475
AfaI GTAC 2 cut(s) 181, 191
AgeI ACCGGT 1 cut(s) 581
AgsI TTSAA 1 cut(s) 92
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 4 cut(s) 38, 145, 489, 563
AluI AGCT 4 cut(s) 38, 145, 489, 563
AlwI GGATC 1 cut(s) 475
ApeKI GCWGC 5 cut(s) 29, 142, 384, 449, 560
AsiGI ACCGGT 1 cut(s) 581
AsuHPI GGTGA 2 cut(s) 122, 392
BbvI GCAGC 5 cut(s) 16, 154, 396, 436, 572
BccI CCATC 5 cut(s) 101, 107, 203, 275, 500
BceAI ACGGC 2 cut(s) 178, 372
BfaI CTAG 2 cut(s) 194, 627
BglII AGATCT 1 cut(s) 545
BisI GCNGC 6 cut(s) 30, 143, 385, 450, 561, 624
BlsI GCNGC 6 cut(s) 31, 144, 386, 451, 562, 625
BmsI GCATC 2 cut(s) 16, 275
BpuEI CTTGAG 1 cut(s) 539
BsaJI CCNNGG 2 cut(s) 126, 444
BsaWI WCCGGW 1 cut(s) 581
Bse118I RCCGGY 1 cut(s) 581
Bse1I ACTGG 1 cut(s) 187
BseDI CCNNGG 2 cut(s) 126, 444
BseGI GGATG 1 cut(s) 290
BseMII CTCAG 1 cut(s) 275
BseNI ACTGG 1 cut(s) 187
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 5 cut(s) 16, 154, 396, 436, 572
BshTI ACCGGT 1 cut(s) 581
BsiSI CCGG 1 cut(s) 582
Bsp143I GATC 5 cut(s) 361, 467, 514, 545, 591
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 2 cut(s) 382, 624
BspCNI CTCAG 1 cut(s) 274
BspPI GGATC 1 cut(s) 475
BsrFI RCCGGY 1 cut(s) 581
BsrI ACTGG 1 cut(s) 187
BssAI RCCGGY 1 cut(s) 581
BssECI CCNNGG 2 cut(s) 126, 444
BssMI GATC 5 cut(s) 361, 467, 514, 545, 591
BssNAI GTATAC 1 cut(s) 81
BssT1I CCWWGG 2 cut(s) 126, 444
Bst1107I GTATAC 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 375
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 1 cut(s) 261
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 290
BstKTI GATC 5 cut(s) 364, 470, 517, 548, 594
BstMBI GATC 5 cut(s) 361, 467, 514, 545, 591
BstMWI GCNNNNNNNGC 8 cut(s) 35, 285, 330, 375, 384, 449, 569, 623
BstV1I GCAGC 5 cut(s) 16, 154, 396, 436, 572
BstX2I RGATCY 1 cut(s) 545
BstYI RGATCY 1 cut(s) 545
BstZ17I GTATAC 1 cut(s) 81
BtgI CCRYGG 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 290
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 1 cut(s) 441
Cfr10I RCCGGY 1 cut(s) 581
Csp6I GTAC 2 cut(s) 180, 190
CspAI ACCGGT 1 cut(s) 581
CviAII CATG 4 cut(s) 71, 127, 170, 633
CviJI RGCY 8 cut(s) 38, 145, 387, 449, 481, 489, 563, 572
CviKI_1 RGCY 8 cut(s) 38, 145, 387, 449, 481, 489, 563, 572
CviQI GTAC 2 cut(s) 180, 190
DdeI CTNAG 1 cut(s) 261
DpnI GATC 5 cut(s) 363, 469, 516, 547, 593
DpnII GATC 5 cut(s) 361, 467, 514, 545, 591
Eco130I CCWWGG 2 cut(s) 126, 444
EcoT14I CCWWGG 2 cut(s) 126, 444
EcoT22I ATGCAT 1 cut(s) 638
ErhI CCWWGG 2 cut(s) 126, 444
FaeI CATG 4 cut(s) 74, 130, 173, 636
FatI CATG 4 cut(s) 70, 126, 169, 632
FblI GTMKAC 1 cut(s) 80
Fnu4HI GCNGC 6 cut(s) 30, 143, 385, 450, 561, 624
FokI GGATG 1 cut(s) 297
Fsp4HI GCNGC 6 cut(s) 30, 143, 385, 450, 561, 624
FspBI CTAG 2 cut(s) 194, 627
GluI GCNGC 6 cut(s) 30, 143, 385, 450, 561, 624
HapII CCGG 1 cut(s) 582
Hin1II CATG 4 cut(s) 74, 130, 173, 636
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 2 cut(s) 230, 259
HpaII CCGG 1 cut(s) 582
HphI GGTGA 2 cut(s) 122, 392
Hpy166II GTNNAC 3 cut(s) 81, 175, 400
Hpy188I TCNGA 3 cut(s) 58, 264, 596
Hpy188III TCNNGA 5 cut(s) 92, 465, 518, 543, 566
Hpy8I GTNNAC 3 cut(s) 81, 175, 400
HpyAV CCTTC 2 cut(s) 193, 583
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 4 cut(s) 288, 369, 411, 636
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 285, 330, 375, 384, 449, 569, 623
HpyF3I CTNAG 1 cut(s) 261
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 4 cut(s) 74, 130, 173, 636
Kzo9I GATC 5 cut(s) 361, 467, 514, 545, 591
LpnPI CCDG 6 cut(s) 200, 388, 450, 491, 551, 595
Lsp1109I GCAGC 5 cut(s) 16, 154, 396, 436, 572
LweI GCATC 2 cut(s) 16, 275
MaeI CTAG 2 cut(s) 194, 627
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 3 cut(s) 158, 235, 389
MalI GATC 5 cut(s) 363, 469, 516, 547, 593
MboI GATC 5 cut(s) 361, 467, 514, 545, 591
MboII GAAGA 1 cut(s) 277
MflI RGATCY 1 cut(s) 545
MluCI AATT 3 cut(s) 49, 460, 554
MlyI GAGTC 1 cut(s) 253
MnlI CCTC 5 cut(s) 67, 124, 145, 220, 382
Mph1103I ATGCAT 1 cut(s) 638
MslI CAYNNNNRTG 2 cut(s) 323, 641
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 1 cut(s) 582
MwoI GCNNNNNNNGC 8 cut(s) 35, 285, 330, 375, 384, 449, 569, 623
NcoI CCATGG 1 cut(s) 126
NdeII GATC 5 cut(s) 361, 467, 514, 545, 591
NlaIII CATG 4 cut(s) 74, 130, 173, 636
NmeAIII GCCGAG 1 cut(s) 470
NmuCI GTSAC 1 cut(s) 235
NsiI ATGCAT 1 cut(s) 638
OliI CACNNNNGTG 1 cut(s) 323
PcsI WCGNNNNNNNCGW 1 cut(s) 497
PfeI GAWTC 1 cut(s) 230
PflFI GACNNNGTC 1 cut(s) 170
PinAI ACCGGT 1 cut(s) 581
PkrI GCNGC 6 cut(s) 31, 144, 386, 451, 562, 625
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PsiI TTATAA 1 cut(s) 456
PsuI RGATCY 1 cut(s) 545
PsyI GACNNNGTC 1 cut(s) 170
PvuII CAGCTG 1 cut(s) 145
RsaI GTAC 2 cut(s) 181, 191
RsaNI GTAC 2 cut(s) 180, 190
RseI CAYNNNNRTG 2 cut(s) 323, 641
SatI GCNGC 6 cut(s) 30, 143, 385, 450, 561, 624
Sau3AI GATC 5 cut(s) 361, 467, 514, 545, 591
SchI GAGTC 1 cut(s) 253
SfaNI GCATC 2 cut(s) 16, 275
SmiMI CAYNNNNRTG 2 cut(s) 323, 641
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
Sse9I AATT 3 cut(s) 49, 460, 554
SsiI CCGC 2 cut(s) 382, 624
SspI AATATT 1 cut(s) 613
SspMI CTAG 2 cut(s) 194, 627
StyI CCWWGG 2 cut(s) 126, 444
TaaI ACNGT 1 cut(s) 179
TaiI ACGT 1 cut(s) 399
TaqI TCGA 5 cut(s) 138, 336, 470, 500, 542
TasI AATT 3 cut(s) 49, 460, 554
TauI GCSGC 1 cut(s) 626
TfiI GAWTC 1 cut(s) 230
TscAI CASTG 1 cut(s) 413
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 5 cut(s) 29, 142, 384, 449, 560
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 2 cut(s) 543, 621
TspGWI ACGGA 1 cut(s) 11
TspRI CASTG 1 cut(s) 413
Tth111I GACNNNGTC 1 cut(s) 170
XmiI GTMKAC 1 cut(s) 80
XspI CTAG 2 cut(s) 194, 627
Zsp2I ATGCAT 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.