EF hand
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 537 |
| AccII | CGCG | 1 cut(s) | 600 |
| AciI | CCGC | 3 cut(s) | 205, 297, 521 |
| AclWI | GGATC | 2 cut(s) | 75, 173 |
| AfaI | GTAC | 3 cut(s) | 272, 303, 646 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 94 |
| AgsI | TTSAA | 2 cut(s) | 136, 193 |
| AjnI | CCWGG | 1 cut(s) | 588 |
| AleI | CACNNNNGTG | 1 cut(s) | 309 |
| AluBI | AGCT | 4 cut(s) | 26, 61, 123, 350 |
| AluI | AGCT | 4 cut(s) | 26, 61, 123, 350 |
| Alw21I | GWGCWC | 1 cut(s) | 352 |
| AlwI | GGATC | 2 cut(s) | 75, 173 |
| AoxI | GGCC | 1 cut(s) | 469 |
| AseI | ATTAAT | 1 cut(s) | 546 |
| AspLEI | GCGC | 1 cut(s) | 459 |
| AsuHPI | GGTGA | 1 cut(s) | 404 |
| BanII | GRGCYC | 1 cut(s) | 352 |
| Bbv12I | GWGCWC | 1 cut(s) | 352 |
| BccI | CCATC | 3 cut(s) | 104, 209, 604 |
| BciT130I | CCWGG | 1 cut(s) | 590 |
| BglII | AGATCT | 1 cut(s) | 493 |
| Bme1390I | CCNGG | 1 cut(s) | 590 |
| BmiI | GGNNCC | 1 cut(s) | 587 |
| BmrFI | CCNGG | 1 cut(s) | 590 |
| BmsI | GCATC | 1 cut(s) | 367 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 347, 379 |
| BpmI | CTGGAG | 2 cut(s) | 131, 510 |
| BpuEI | CTTGAG | 1 cut(s) | 626 |
| BsaJI | CCNNGG | 1 cut(s) | 283 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 508, 538 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 94 |
| Bse1I | ACTGG | 1 cut(s) | 170 |
| Bse3DI | GCAATG | 3 cut(s) | 12, 214, 513 |
| BseBI | CCWGG | 1 cut(s) | 590 |
| BseDI | CCNNGG | 1 cut(s) | 283 |
| BseGI | GGATG | 1 cut(s) | 115 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 94 |
| BseMI | GCAATG | 3 cut(s) | 12, 214, 513 |
| BseNI | ACTGG | 1 cut(s) | 170 |
| BsgI | GTGCAG | 1 cut(s) | 549 |
| Bsh1236I | CGCG | 1 cut(s) | 600 |
| BshFI | GGCC | 1 cut(s) | 471 |
| BsiHKAI | GWGCWC | 1 cut(s) | 352 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 94 |
| BsnI | GGCC | 1 cut(s) | 471 |
| Bsp1286I | GDGCHC | 1 cut(s) | 352 |
| Bsp1407I | TGTACA | 2 cut(s) | 301, 644 |
| Bsp143I | GATC | 5 cut(s) | 67, 89, 178, 373, 493 |
| BspACI | CCGC | 3 cut(s) | 205, 297, 521 |
| BspANI | GGCC | 1 cut(s) | 471 |
| BspFNI | CGCG | 1 cut(s) | 600 |
| BspHI | TCATGA | 1 cut(s) | 352 |
| BspLI | GGNNCC | 1 cut(s) | 587 |
| BspPI | GGATC | 2 cut(s) | 75, 173 |
| BsrDI | GCAATG | 3 cut(s) | 12, 214, 513 |
| BsrGI | TGTACA | 2 cut(s) | 301, 644 |
| BsrI | ACTGG | 1 cut(s) | 170 |
| BssECI | CCNNGG | 1 cut(s) | 283 |
| BssMI | GATC | 5 cut(s) | 67, 89, 178, 373, 493 |
| BssT1I | CCWWGG | 1 cut(s) | 283 |
| Bst2UI | CCWGG | 1 cut(s) | 590 |
| BstAUI | TGTACA | 2 cut(s) | 301, 644 |
| BstC8I | GCNNGC | 3 cut(s) | 337, 388, 602 |
| BstF5I | GGATG | 1 cut(s) | 115 |
| BstFNI | CGCG | 1 cut(s) | 600 |
| BstHHI | GCGC | 1 cut(s) | 459 |
| BstKTI | GATC | 5 cut(s) | 70, 92, 181, 376, 496 |
| BstMBI | GATC | 5 cut(s) | 67, 89, 178, 373, 493 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 32, 336, 620, 632 |
| BstNI | CCWGG | 1 cut(s) | 590 |
| BstNSI | RCATGY | 1 cut(s) | 23 |
| BstSCI | CCNGG | 1 cut(s) | 588 |
| BstUI | CGCG | 1 cut(s) | 600 |
| BstX2I | RGATCY | 2 cut(s) | 67, 493 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 155 |
| BstYI | RGATCY | 2 cut(s) | 67, 493 |
| BsuRI | GGCC | 1 cut(s) | 471 |
| BtgZI | GCGATG | 3 cut(s) | 228, 344, 541 |
| BtsCI | GGATG | 1 cut(s) | 115 |
| Cac8I | GCNNGC | 3 cut(s) | 337, 388, 602 |
| CciI | TCATGA | 1 cut(s) | 352 |
| CfoI | GCGC | 1 cut(s) | 459 |
| Csp6I | GTAC | 3 cut(s) | 271, 302, 645 |
| CviAII | CATG | 5 cut(s) | 20, 353, 446, 502, 652 |
| CviJI | RGCY | 8 cut(s) | 26, 61, 123, 159, 350, 471, 614, 635 |
| CviKI_1 | RGCY | 8 cut(s) | 26, 61, 123, 159, 350, 471, 614, 635 |
| CviQI | GTAC | 3 cut(s) | 271, 302, 645 |
| DpnI | GATC | 5 cut(s) | 69, 91, 180, 375, 495 |
| DpnII | GATC | 5 cut(s) | 67, 89, 178, 373, 493 |
| Ecl136II | GAGCTC | 1 cut(s) | 350 |
| Eco130I | CCWWGG | 1 cut(s) | 283 |
| Eco147I | AGGCCT | 1 cut(s) | 471 |
| Eco24I | GRGCYC | 1 cut(s) | 352 |
| Eco53kI | GAGCTC | 1 cut(s) | 350 |
| EcoICRI | GAGCTC | 1 cut(s) | 350 |
| EcoRII | CCWGG | 1 cut(s) | 588 |
| EcoT14I | CCWWGG | 1 cut(s) | 283 |
| EcoT38I | GRGCYC | 1 cut(s) | 352 |
| ErhI | CCWWGG | 1 cut(s) | 283 |
| FaeI | CATG | 5 cut(s) | 23, 356, 449, 505, 655 |
| FatI | CATG | 5 cut(s) | 19, 352, 445, 501, 651 |
| FauI | CCCGC | 1 cut(s) | 528 |
| FauNDI | CATATG | 1 cut(s) | 481 |
| FokI | GGATG | 1 cut(s) | 122 |
| FriOI | GRGCYC | 1 cut(s) | 352 |
| GlaI | GCGC | 1 cut(s) | 458 |
| GsuI | CTGGAG | 2 cut(s) | 131, 510 |
| HaeIII | GGCC | 1 cut(s) | 471 |
| HhaI | GCGC | 1 cut(s) | 459 |
| Hin1II | CATG | 5 cut(s) | 23, 356, 449, 505, 655 |
| Hin6I | GCGC | 1 cut(s) | 457 |
| HinP1I | GCGC | 1 cut(s) | 457 |
| HinfI | GANTC | 1 cut(s) | 511 |
| HphI | GGTGA | 1 cut(s) | 404 |
| Hpy166II | GTNNAC | 2 cut(s) | 302, 304 |
| Hpy188I | TCNGA | 2 cut(s) | 89, 402 |
| Hpy188III | TCNNGA | 2 cut(s) | 65, 353 |
| Hpy8I | GTNNAC | 2 cut(s) | 302, 304 |
| HpyCH4V | TGCA | 3 cut(s) | 23, 566, 626 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 32, 336, 620, 632 |
| Hsp92II | CATG | 5 cut(s) | 23, 356, 449, 505, 655 |
| HspAI | GCGC | 1 cut(s) | 457 |
| Kzo9I | GATC | 5 cut(s) | 67, 89, 178, 373, 493 |
| LmnI | GCTCC | 4 cut(s) | 347, 368, 464, 490 |
| LweI | GCATC | 1 cut(s) | 367 |
| MaeIII | GTNAC | 1 cut(s) | 404 |
| MalI | GATC | 5 cut(s) | 69, 91, 180, 375, 495 |
| MboI | GATC | 5 cut(s) | 67, 89, 178, 373, 493 |
| MboII | GAAGA | 3 cut(s) | 23, 76, 181 |
| MflI | RGATCY | 2 cut(s) | 67, 493 |
| MhlI | GDGCHC | 1 cut(s) | 352 |
| MluCI | AATT | 2 cut(s) | 547, 640 |
| MlyI | GAGTC | 1 cut(s) | 505 |
| MmeI | TCCRAC | 1 cut(s) | 539 |
| MnlI | CCTC | 7 cut(s) | 155, 261, 317, 461, 482, 534, 579 |
| MseI | TTAA | 3 cut(s) | 174, 261, 546 |
| MslI | CAYNNNNRTG | 2 cut(s) | 309, 506 |
| MspA1I | CMGCKG | 2 cut(s) | 26, 123 |
| MspR9I | CCNGG | 1 cut(s) | 590 |
| MvaI | CCWGG | 1 cut(s) | 590 |
| MvnI | CGCG | 1 cut(s) | 600 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 32, 336, 620, 632 |
| NdeI | CATATG | 1 cut(s) | 481 |
| NdeII | GATC | 5 cut(s) | 67, 89, 178, 373, 493 |
| NlaIII | CATG | 5 cut(s) | 23, 356, 449, 505, 655 |
| NlaIV | GGNNCC | 1 cut(s) | 587 |
| NspI | RCATGY | 1 cut(s) | 23 |
| OliI | CACNNNNGTG | 1 cut(s) | 309 |
| PagI | TCATGA | 1 cut(s) | 352 |
| PceI | AGGCCT | 1 cut(s) | 471 |
| PleI | GAGTC | 1 cut(s) | 505 |
| PpsI | GAGTC | 1 cut(s) | 505 |
| PshBI | ATTAAT | 1 cut(s) | 546 |
| PsiI | TTATAA | 1 cut(s) | 537 |
| Psp124BI | GAGCTC | 1 cut(s) | 352 |
| Psp6I | CCWGG | 1 cut(s) | 588 |
| PspGI | CCWGG | 1 cut(s) | 588 |
| PspN4I | GGNNCC | 1 cut(s) | 587 |
| PsuI | RGATCY | 2 cut(s) | 67, 493 |
| PvuII | CAGCTG | 2 cut(s) | 26, 123 |
| RsaI | GTAC | 3 cut(s) | 272, 303, 646 |
| RsaNI | GTAC | 3 cut(s) | 271, 302, 645 |
| RseI | CAYNNNNRTG | 2 cut(s) | 309, 506 |
| SacI | GAGCTC | 1 cut(s) | 352 |
| SaqAI | TTAA | 3 cut(s) | 174, 261, 546 |
| Sau3AI | GATC | 5 cut(s) | 67, 89, 178, 373, 493 |
| SchI | GAGTC | 1 cut(s) | 505 |
| ScrFI | CCNGG | 1 cut(s) | 590 |
| SduI | GDGCHC | 1 cut(s) | 352 |
| SetI | ASST | 9 cut(s) | 28, 63, 125, 272, 309, 352, 371, 571, 594 |
| SfaNI | GCATC | 1 cut(s) | 367 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 309, 506 |
| SmlI | CTYRAG | 1 cut(s) | 605 |
| SmoI | CTYRAG | 1 cut(s) | 605 |
| Sse9I | AATT | 2 cut(s) | 547, 640 |
| SseBI | AGGCCT | 1 cut(s) | 471 |
| SsiI | CCGC | 3 cut(s) | 205, 297, 521 |
| SspI | AATATT | 1 cut(s) | 544 |
| SstI | GAGCTC | 1 cut(s) | 352 |
| StuI | AGGCCT | 1 cut(s) | 471 |
| StyD4I | CCNGG | 1 cut(s) | 588 |
| StyI | CCWWGG | 1 cut(s) | 283 |
| TasI | AATT | 2 cut(s) | 547, 640 |
| TatI | WGTACW | 2 cut(s) | 301, 644 |
| Tru1I | TTAA | 3 cut(s) | 174, 261, 546 |
| Tru9I | TTAA | 3 cut(s) | 174, 261, 546 |
| TspGWI | ACGGA | 1 cut(s) | 360 |
| VspI | ATTAAT | 1 cut(s) | 546 |
| XceI | RCATGY | 1 cut(s) | 23 |