pycom13g19680

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000286
Physical Location & Seq
Reverse (-)
15637371 .. 15638487
1117 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g19680.1

Sequence Viewer

Length: 726 bp
ATGGAGGATTTGCGCCCAACAGCATTTGCATACTACAAGTATGCGCCGACGGATGTTCAACGGTTGGTAGATGAGACTTTCTACGAAATGGATCTAGACAAGAACGACCGTGTGAGCTGGCAAGAGTTCACGAAATACATGGAGATGCATGGAGATTGCAAGCATCTGTGTAACTCTCATTTTTTCGACGAACTAAAGAAGGAAGGACATGAAGAGCTTGATTTCATGGACGTTATGACTCTCTTCTACATTGTTTATAGTGGAAAACCTTTTTGTAACGGGCAGTGCAGAAAATTTATCAAGGGAGTTTATTTCACCTGTGTCAAATGCTTTGATGATGATGCCACTGATGCCACCAACACGTTCAATGTTTGCCCTGCTTGTTATGTTGATGGAAAATATGTCCATGGCCACGACAAATTCCTTGATAACTTCCTGCTTCTTCAAAGGAACAGGATGACAGCTTTGAACCAACAAGCAGCGCCAGTCACTAATAAGTCAAAGGCACCCAAGTATGGAAGTACATCGGAGACAAGCAATTCGTCTTCTCCAGCTCCTCAAGCTCCTCAAGCTCCTCCTCGTCCACTCACACCTCATACATCCAATGCAATGGTTCCCGTTAATCCAATGCCAAAAAATAGTGCCGGGAGGAAAGCATTGAAGACGATAGAAATCCTACTGGCCCTGGGAAATATCTTTGCTGCCACTCAGTGCACCATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5

Protein Analysis

242

Amino Acids

27.32

Weight (kDa)

6.38

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 505
AclWI GGATC 1 cut(s) 99
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 2 cut(s) 293, 419
AdeI CACNNNGTG 1 cut(s) 711
AfaI GTAC 1 cut(s) 523
AfiI CCNNNNNNNGG 1 cut(s) 515
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 5 cut(s) 59, 367, 446, 469, 661
AjnI CCWGG 1 cut(s) 684
AluBI AGCT 6 cut(s) 117, 217, 464, 554, 563, 572
AluI AGCT 6 cut(s) 117, 217, 464, 554, 563, 572
Alw21I GWGCWC 1 cut(s) 716
Alw26I GTCTC 2 cut(s) 68, 524
Alw44I GTGCAC 1 cut(s) 712
AlwI GGATC 1 cut(s) 99
AoxI GGCC 2 cut(s) 409, 681
ApaLI GTGCAC 1 cut(s) 712
ApeKI GCWGC 2 cut(s) 479, 701
ApoI RAATTY 2 cut(s) 293, 419
ArsI GACNNNNNNTTYG 2 cut(s) 523, 555
AspLEI GCGC 3 cut(s) 15, 46, 484
AspS9I GGNCC 1 cut(s) 682
AsuC2I CCSGG 1 cut(s) 646
AsuHPI GGTGA 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 716
BalI TGGCCA 1 cut(s) 411
BanI GGYRCC 1 cut(s) 505
BbsI GAAGAC 2 cut(s) 537, 668
Bbv12I GWGCWC 1 cut(s) 716
BbvI GCAGC 2 cut(s) 491, 688
BccI CCATC 2 cut(s) 386, 725
BciT130I CCWGG 1 cut(s) 686
BcnI CCSGG 1 cut(s) 646
BcoDI GTCTC 2 cut(s) 68, 524
BfaI CTAG 1 cut(s) 95
BfoI RGCGCY 1 cut(s) 485
BisI GCNGC 2 cut(s) 480, 702
BlsI GCNGC 2 cut(s) 481, 703
Bme1390I CCNGG 2 cut(s) 646, 686
BmgT120I GGNCC 1 cut(s) 682
BmiI GGNNCC 2 cut(s) 507, 615
BmrFI CCNGG 2 cut(s) 646, 686
BmsI GCATC 4 cut(s) 135, 172, 331, 340
BpiI GAAGAC 2 cut(s) 537, 668
BpmI CTGGAG 1 cut(s) 534
BpuEI CTTGAG 2 cut(s) 543, 552
BpuMI CCSGG 1 cut(s) 646
BsaBI GATNNNNATC 1 cut(s) 671
BsaJI CCNNGG 3 cut(s) 406, 684, 685
Bsc4I CCNNNNNNNGG 1 cut(s) 515
Bse1I ACTGG 2 cut(s) 485, 684
Bse3DI GCAATG 1 cut(s) 615
Bse8I GATNNNNATC 1 cut(s) 671
BseBI CCWGG 1 cut(s) 686
BseDI CCNNGG 3 cut(s) 406, 684, 685
BseGI GGATG 3 cut(s) 58, 462, 599
BseJI GATNNNNATC 1 cut(s) 671
BseLI CCNNNNNNNGG 1 cut(s) 515
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 1 cut(s) 722
BseNI ACTGG 2 cut(s) 485, 684
BseRI GAGGAG 4 cut(s) 546, 555, 564, 567
BseSI GKGCMC 1 cut(s) 716
BseXI GCAGC 2 cut(s) 491, 688
BsgI GTGCAG 1 cut(s) 307
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 2 cut(s) 411, 683
BshNI GGYRCC 1 cut(s) 505
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 716
BsiSI CCGG 1 cut(s) 645
BslI CCNNNNNNNGG 1 cut(s) 515
BsmAI GTCTC 2 cut(s) 68, 524
BsnI GGCC 2 cut(s) 411, 683
Bsp1286I GDGCHC 1 cut(s) 716
Bsp143I GATC 1 cut(s) 91
Bsp19I CCATGG 1 cut(s) 406
BspANI GGCC 2 cut(s) 411, 683
BspCNI CTCAG 1 cut(s) 721
BspLI GGNNCC 2 cut(s) 507, 615
BspPI GGATC 1 cut(s) 99
BspQI GCTCTTC 1 cut(s) 207
BspT107I GGYRCC 1 cut(s) 505
BsrDI GCAATG 1 cut(s) 615
BsrI ACTGG 2 cut(s) 485, 684
BssECI CCNNGG 3 cut(s) 406, 684, 685
BssMI GATC 1 cut(s) 91
BssT1I CCWWGG 1 cut(s) 406
Bst2UI CCWGG 1 cut(s) 686
Bst4CI ACNGT 2 cut(s) 63, 110
Bst6I CTCTTC 2 cut(s) 207, 248
BstC8I GCNNGC 2 cut(s) 119, 161
BstDEI CTNAG 1 cut(s) 708
BstDSI CCRYGG 1 cut(s) 406
BstF5I GGATG 3 cut(s) 58, 462, 599
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 3 cut(s) 15, 46, 484
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 2 cut(s) 68, 524
BstMBI GATC 1 cut(s) 91
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 3 cut(s) 350, 560, 569
BstNI CCWGG 1 cut(s) 686
BstSCI CCNGG 2 cut(s) 644, 684
BstSLI GKGCMC 1 cut(s) 716
BstV1I GCAGC 2 cut(s) 491, 688
BstV2I GAAGAC 2 cut(s) 537, 668
BstX2I RGATCY 1 cut(s) 91
BstXI CCANNNNNNTGG 1 cut(s) 610
BstYI RGATCY 1 cut(s) 91
BsuRI GGCC 2 cut(s) 411, 683
BtgI CCRYGG 1 cut(s) 406
BtsCI GGATG 3 cut(s) 58, 462, 599
BtsI GCAGTG 1 cut(s) 290
BtsIMutI CAGTG 3 cut(s) 290, 345, 716
Cac8I GCNNGC 2 cut(s) 119, 161
CfoI GCGC 3 cut(s) 15, 46, 484
Cfr13I GGNCC 1 cut(s) 682
Csp6I GTAC 1 cut(s) 522
CviAII CATG 6 cut(s) 139, 149, 209, 226, 407, 721
CviJI RGCY 8 cut(s) 117, 217, 411, 464, 554, 563, 572, 683
CviKI_1 RGCY 8 cut(s) 117, 217, 411, 464, 554, 563, 572, 683
CviQI GTAC 1 cut(s) 522
DdeI CTNAG 1 cut(s) 708
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
DraIII CACNNNGTG 1 cut(s) 711
EaeI YGGCCR 1 cut(s) 409
Eam1104I CTCTTC 2 cut(s) 207, 248
EarI CTCTTC 2 cut(s) 207, 248
Eco130I CCWWGG 1 cut(s) 406
EcoRII CCWGG 1 cut(s) 684
EcoT14I CCWWGG 1 cut(s) 406
EcoT22I ATGCAT 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 6 cut(s) 142, 152, 212, 229, 410, 724
FatI CATG 6 cut(s) 138, 148, 208, 225, 406, 720
Fnu4HI GCNGC 2 cut(s) 480, 702
FokI GGATG 3 cut(s) 65, 469, 586
Fsp4HI GCNGC 2 cut(s) 480, 702
FspBI CTAG 1 cut(s) 95
GlaI GCGC 3 cut(s) 14, 45, 483
GluI GCNGC 2 cut(s) 480, 702
GsuI CTGGAG 1 cut(s) 534
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 2 cut(s) 411, 683
HapII CCGG 1 cut(s) 645
HhaI GCGC 3 cut(s) 15, 46, 484
Hin1II CATG 6 cut(s) 142, 152, 212, 229, 410, 724
Hin6I GCGC 3 cut(s) 13, 44, 482
HinP1I GCGC 3 cut(s) 13, 44, 482
HinfI GANTC 1 cut(s) 238
HpaII CCGG 1 cut(s) 645
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 3 cut(s) 129, 584, 714
Hpy188I TCNGA 1 cut(s) 529
Hpy188III TCNNGA 2 cut(s) 95, 130
Hpy8I GTNNAC 3 cut(s) 129, 584, 714
Hpy99I CGWCG 2 cut(s) 52, 191
HpyAV CCTTC 2 cut(s) 193, 197
HpyCH4III ACNGT 2 cut(s) 63, 110
HpyCH4IV ACGT 2 cut(s) 231, 362
HpyCH4V TGCA 6 cut(s) 29, 148, 159, 288, 608, 714
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 560, 569
HpyF3I CTNAG 1 cut(s) 708
HpySE526I ACGT 2 cut(s) 231, 362
Hsp92II CATG 6 cut(s) 142, 152, 212, 229, 410, 724
HspAI GCGC 3 cut(s) 13, 44, 482
Kzo9I GATC 1 cut(s) 91
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 3 cut(s) 559, 568, 577
Lsp1109I GCAGC 2 cut(s) 491, 688
LweI GCATC 4 cut(s) 135, 172, 331, 340
MaeI CTAG 1 cut(s) 95
MaeII ACGT 2 cut(s) 231, 362
MaeIII GTNAC 3 cut(s) 170, 275, 487
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 5 cut(s) 224, 235, 434, 537, 673
MflI RGATCY 1 cut(s) 91
MhlI GDGCHC 1 cut(s) 716
MlsI TGGCCA 1 cut(s) 411
MluCI AATT 3 cut(s) 293, 419, 538
MluNI TGGCCA 1 cut(s) 411
MlyI GAGTC 1 cut(s) 232
MnlI CCTC 6 cut(s) 567, 576, 585, 588, 603, 642
Mox20I TGGCCA 1 cut(s) 411
Mph1103I ATGCAT 1 cut(s) 150
MscI TGGCCA 1 cut(s) 411
MseI TTAA 1 cut(s) 621
MslI CAYNNNNRTG 2 cut(s) 143, 719
Msp20I TGGCCA 1 cut(s) 411
MspI CCGG 1 cut(s) 645
MspR9I CCNGG 2 cut(s) 646, 686
MvaI CCWGG 1 cut(s) 686
MwoI GCNNNNNNNGC 3 cut(s) 350, 560, 569
NciI CCSGG 1 cut(s) 646
NcoI CCATGG 1 cut(s) 406
NdeII GATC 1 cut(s) 91
NlaIII CATG 6 cut(s) 142, 152, 212, 229, 410, 724
NlaIV GGNNCC 2 cut(s) 507, 615
NmuCI GTSAC 1 cut(s) 487
NsiI ATGCAT 1 cut(s) 150
PasI CCCWGGG 1 cut(s) 685
PciSI GCTCTTC 1 cut(s) 207
PkrI GCNGC 2 cut(s) 481, 703
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 684
PspGI CCWGG 1 cut(s) 684
PspN4I GGNNCC 2 cut(s) 507, 615
PspPI GGNCC 1 cut(s) 682
PsuI RGATCY 1 cut(s) 91
RsaI GTAC 1 cut(s) 523
RsaNI GTAC 1 cut(s) 522
RseI CAYNNNNRTG 2 cut(s) 143, 719
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 2 cut(s) 480, 702
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 1 cut(s) 682
SchI GAGTC 1 cut(s) 232
ScrFI CCNGG 2 cut(s) 646, 686
SduI GDGCHC 1 cut(s) 716
SfaNI GCATC 4 cut(s) 135, 172, 331, 340
SmiMI CAYNNNNRTG 2 cut(s) 143, 719
SmlI CTYRAG 2 cut(s) 558, 567
SmoI CTYRAG 2 cut(s) 558, 567
Sse9I AATT 3 cut(s) 293, 419, 538
SspMI CTAG 1 cut(s) 95
StyD4I CCNGG 2 cut(s) 644, 684
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 2 cut(s) 63, 110
TaiI ACGT 2 cut(s) 234, 365
TaqI TCGA 1 cut(s) 186
TasI AATT 3 cut(s) 293, 419, 538
TatI WGTACW 1 cut(s) 521
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TscAI CASTG 3 cut(s) 290, 352, 716
TseFI GTSAC 1 cut(s) 487
TseI GCWGC 2 cut(s) 479, 701
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 2 cut(s) 214, 225
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 3 cut(s) 290, 352, 716
VneI GTGCAC 1 cut(s) 712
XapI RAATTY 2 cut(s) 293, 419
XbaI TCTAGA 1 cut(s) 94
XspI CTAG 1 cut(s) 95
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.