Rw4G005120

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000286
Physical Location & Seq
Forward (+)
10417861 .. 10419143
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G005120.1

Sequence Viewer

Length: 564 bp
ATGGCAATGGAAGAACTACATGCAGCTGCTTTAGCATACTACGAGAATAGCACTCAAAGGACTTGGGATCTGGCAAGGTCTTTCTTCCAATCTATGGACACCAACGGTGATGGTCGAATCAGCCACTCGGAACTCGACCTCAATGGGGATGGTGGATTGGATTTTGGGGAAGTTCTCACTTTCCACTACATCATTAAAATGAGGATCCTCCGATGCCAAGGGTCCCCGTGCGGCGCACAGTACCTCTTCGGTCTGTATTTCACTTGCGTTGCTTGCTTTGACGGAGCACATGGGCATACCTTTGATCTCTGTCCTGCCTGCTATCGCAATCGGAGTTACCAGCACCACACATACTTCTTGGAAAATCATGTATTGCTGCGCTCCAAACGAGGCCTCTCTCCTCAACCAGATCTCAACATGGCAATCACTCCACCCCCAGCCCCGCCTCCATCAGTTTATACTATCATTAATAATTACTACATTGCTGCTGAACCCCCAAAGGGTGACGAGTTTGAGGCGGTTCTTACTGCTGCAAGTGTGGTTTCTAATTGTACAATCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

187

Amino Acids

20.88

Weight (kDa)

5.51

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 94
AciI CCGC 3 cut(s) 231, 443, 518
AclWI GGATC 3 cut(s) 75, 199, 212
AfaI GTAC 2 cut(s) 242, 553
AfiI CCNNNNNNNGG 3 cut(s) 94, 145, 500
AluBI AGCT 1 cut(s) 26
AluI AGCT 1 cut(s) 26
Alw21I GWGCWC 1 cut(s) 289
AlwI GGATC 3 cut(s) 75, 199, 212
AoxI GGCC 1 cut(s) 391
ApeKI GCWGC 5 cut(s) 23, 26, 376, 485, 530
AseI ATTAAT 1 cut(s) 468
AspLEI GCGC 2 cut(s) 236, 381
AspS9I GGNCC 1 cut(s) 222
AsuHPI GGTGA 2 cut(s) 119, 515
AvaII GGWCC 1 cut(s) 222
BamHI GGATCC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 289
BbvI GCAGC 5 cut(s) 13, 35, 363, 472, 517
BccI CCATC 3 cut(s) 104, 143, 457
BglII AGATCT 1 cut(s) 409
BisI GCNGC 6 cut(s) 24, 27, 232, 377, 486, 531
BlsI GCNGC 6 cut(s) 25, 28, 233, 378, 487, 532
Bme18I GGWCC 1 cut(s) 222
BmgT120I GGNCC 1 cut(s) 222
BmiI GGNNCC 3 cut(s) 206, 223, 224
BmsI GCATC 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 217
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 145, 500
Bse3DI GCAATG 2 cut(s) 12, 480
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 3 cut(s) 94, 145, 500
BseMI GCAATG 2 cut(s) 12, 480
BseRI GAGGAG 1 cut(s) 390
BseXI GCAGC 5 cut(s) 13, 35, 363, 472, 517
BseYI CCCAGC 1 cut(s) 436
BshFI GGCC 1 cut(s) 393
BsiHKAI GWGCWC 1 cut(s) 289
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 3 cut(s) 94, 145, 500
BsmFI GGGAC 1 cut(s) 208
BsnI GGCC 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 289
Bsp1407I TGTACA 1 cut(s) 551
Bsp143I GATC 4 cut(s) 67, 204, 304, 409
BspACI CCGC 3 cut(s) 231, 443, 518
BspANI GGCC 1 cut(s) 393
BspLI GGNNCC 3 cut(s) 206, 223, 224
BspPI GGATC 3 cut(s) 75, 199, 212
BsrDI GCAATG 2 cut(s) 12, 480
BsrGI TGTACA 1 cut(s) 551
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 4 cut(s) 67, 204, 304, 409
BssT1I CCWWGG 1 cut(s) 217
Bst4CI ACNGT 2 cut(s) 107, 240
Bst6I CTCTTC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 551
BstC8I GCNNGC 2 cut(s) 274, 319
BstF5I GGATG 1 cut(s) 154
BstHHI GCGC 2 cut(s) 236, 381
BstKTI GATC 4 cut(s) 70, 207, 307, 412
BstMBI GATC 4 cut(s) 67, 204, 304, 409
BstMWI GCNNNNNNNGC 2 cut(s) 32, 273
BstNSI RCATGY 1 cut(s) 23
BstV1I GCAGC 5 cut(s) 13, 35, 363, 472, 517
BstX2I RGATCY 3 cut(s) 67, 204, 409
BstYI RGATCY 3 cut(s) 67, 204, 409
BsuRI GGCC 1 cut(s) 393
BtsCI GGATG 1 cut(s) 154
Cac8I GCNNGC 2 cut(s) 274, 319
CfoI GCGC 2 cut(s) 236, 381
Cfr13I GGNCC 1 cut(s) 222
Csp6I GTAC 2 cut(s) 241, 552
CviAII CATG 5 cut(s) 20, 290, 368, 418, 559
CviJI RGCY 4 cut(s) 26, 123, 393, 440
CviKI_1 RGCY 4 cut(s) 26, 123, 393, 440
CviQI GTAC 2 cut(s) 241, 552
DpnI GATC 4 cut(s) 69, 206, 306, 411
DpnII GATC 4 cut(s) 67, 204, 304, 409
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 217
Eco147I AGGCCT 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 222
EcoO109I RGGNCCY 1 cut(s) 222
EcoT14I CCWWGG 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 5 cut(s) 23, 293, 371, 421, 562
FaqI GGGAC 1 cut(s) 208
FatI CATG 5 cut(s) 19, 289, 367, 417, 558
FauI CCCGC 1 cut(s) 450
Fnu4HI GCNGC 6 cut(s) 24, 27, 232, 377, 486, 531
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 6 cut(s) 24, 27, 232, 377, 486, 531
GlaI GCGC 2 cut(s) 235, 380
GluI GCNGC 6 cut(s) 24, 27, 232, 377, 486, 531
GsaI CCCAGC 1 cut(s) 440
HaeIII GGCC 1 cut(s) 393
HhaI GCGC 2 cut(s) 236, 381
Hin1II CATG 5 cut(s) 23, 293, 371, 421, 562
Hin6I GCGC 2 cut(s) 234, 379
HinP1I GCGC 2 cut(s) 234, 379
HinfI GANTC 1 cut(s) 117
HphI GGTGA 2 cut(s) 119, 515
Hpy188I TCNGA 3 cut(s) 130, 212, 333
HpyCH4III ACNGT 2 cut(s) 107, 240
HpyCH4V TGCA 2 cut(s) 23, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 273
Hsp92II CATG 5 cut(s) 23, 293, 371, 421, 562
HspAI GCGC 2 cut(s) 234, 379
KflI GGGWCCC 1 cut(s) 222
Kzo9I GATC 4 cut(s) 67, 204, 304, 409
LmnI GCTCC 2 cut(s) 284, 386
LpnPI CCDG 6 cut(s) 56, 327, 331, 353, 420, 450
Lsp1109I GCAGC 5 cut(s) 13, 35, 363, 472, 517
LweI GCATC 1 cut(s) 203
MaeIII GTNAC 2 cut(s) 335, 503
MalI GATC 4 cut(s) 69, 206, 306, 411
MboI GATC 4 cut(s) 67, 204, 304, 409
MboII GAAGA 3 cut(s) 23, 76, 238
MflI RGATCY 3 cut(s) 67, 204, 409
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 2 cut(s) 472, 547
MnlI CCTC 9 cut(s) 149, 195, 218, 254, 383, 404, 411, 456, 508
MseI TTAA 2 cut(s) 195, 468
MslI CAYNNNNRTG 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 32, 273
NdeII GATC 4 cut(s) 67, 204, 304, 409
NlaIII CATG 5 cut(s) 23, 293, 371, 421, 562
NlaIV GGNNCC 3 cut(s) 206, 223, 224
NmuCI GTSAC 1 cut(s) 503
NspI RCATGY 1 cut(s) 23
PceI AGGCCT 1 cut(s) 393
PfeI GAWTC 1 cut(s) 117
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 6 cut(s) 25, 28, 233, 378, 487, 532
PpuMI RGGWCCY 1 cut(s) 222
PshBI ATTAAT 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 222
PspFI CCCAGC 1 cut(s) 436
PspN4I GGNNCC 3 cut(s) 206, 223, 224
PspPI GGNCC 1 cut(s) 222
PspPPI RGGWCCY 1 cut(s) 222
PsuI RGATCY 3 cut(s) 67, 204, 409
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 2 cut(s) 242, 553
RsaNI GTAC 2 cut(s) 241, 552
RseI CAYNNNNRTG 1 cut(s) 197
SaqAI TTAA 2 cut(s) 195, 468
SatI GCNGC 6 cut(s) 24, 27, 232, 377, 486, 531
Sau3AI GATC 4 cut(s) 67, 204, 304, 409
Sau96I GGNCC 1 cut(s) 222
SduI GDGCHC 1 cut(s) 289
SetI ASST 5 cut(s) 28, 80, 141, 246, 302
SfaNI GCATC 1 cut(s) 203
SinI GGWCC 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 197
Sse9I AATT 2 cut(s) 472, 547
SseBI AGGCCT 1 cut(s) 393
SsiI CCGC 3 cut(s) 231, 443, 518
StuI AGGCCT 1 cut(s) 393
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 2 cut(s) 107, 240
TaqI TCGA 2 cut(s) 115, 135
TaqII GACCGA 1 cut(s) 239
TasI AATT 2 cut(s) 472, 547
TatI WGTACW 1 cut(s) 551
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 2 cut(s) 195, 468
Tru9I TTAA 2 cut(s) 195, 468
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 5 cut(s) 23, 26, 376, 485, 530
Tsp45I GTSAC 1 cut(s) 503
TspGWI ACGGA 1 cut(s) 297
Van91I CCANNNNNTGG 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 222
VspI ATTAAT 1 cut(s) 468
XceI RCATGY 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.