Rw4G004870

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000286
Physical Location & Seq
Reverse (-)
9566195 .. 9575274
9080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004870.1

Sequence Viewer

Length: 924 bp
ATGGAAGAAATACGTGAAGTAGCTCTAGCATACTATGAGAATTGCACACCAGAACTTCGGGGTAAGACATGGGAGTTCTTTCAATCCATGGATACGAACGGCGATCACCGAATCAGGCCCAGGGAGTTCAATGAATTCCACCGTCAGACCGGTTACAACACGATCATTACTGACCCAGAATTATTTGCAAAGCTTGGCCGCAATGGTGACAGCGGGCTGGATTTTTCCGAATTTCTCACTTTCTACGCAATGGAATATCTATATGCAGAAGCTTTAGAGTACTATAATAAAGCAACTCCAGAGCTTCAGAGTTCAGTTGTGTCTTTCTTTCAATCCATGGTTACAGACGGTGCAAACCAAATCAGCTCCACTGTGTTCAACGATTTCCTCAAGCAAAAAGGCTACAGCTGGATTATTAATGATGAACACTTGTTCCAGAGACTCCACCGCAACCGTGATGGAAGCCTCGATTTCTGGGAAATGCTCACCTTCTACTACATCATCAGCACAAGGTACTTCCACTGCCGAGAATGTCAGGTGTACCTTAGCGGGCTGTATTTTACCTACGTTGCTTGCTTTGAGGGAGGAGCTCATTATTGTCATCCTCATTCTGATCCCGGCACCTACAATCTATGTGCAGCCTACTATCGCAGTAGGAATTCCATCTCTCAACACCCTCACCACACATACTTCTTGGACAACCACATATTGCTACGCTCTAAAAGAGGTCATCCTCCTTATGCTGCACCACAATTGCCACTACCGGCAGAAGCCAAGCTCGGTATCGAAGCCAACCTTGCGCTAAGCAGCTTCCAATTCTTTGTCACCCACGGCAAGACAGATCAAGTGCCGAAAGTCCCTAGTCCGCTGGACCATGGAGGAGAGGAGACTTGCTGCAACGCGGCCAAGATGACCACAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

307

Amino Acids

35.55

Weight (kDa)

5.81

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 620
AccII CGCG 1 cut(s) 902
AciI CCGC 6 cut(s) 199, 213, 448, 549, 866, 902
AclWI GGATC 1 cut(s) 608
AcoI YGGCCR 2 cut(s) 196, 903
AcsI RAATTY 3 cut(s) 134, 230, 658
AcuI CTGAAG 1 cut(s) 290
AfaI GTAC 3 cut(s) 281, 515, 542
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 4 cut(s) 83, 130, 332, 379
AjnI CCWGG 1 cut(s) 119
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 9 cut(s) 23, 193, 272, 304, 366, 408, 590, 778, 810
AluI AGCT 9 cut(s) 23, 193, 272, 304, 366, 408, 590, 778, 810
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 2 cut(s) 433, 881
AlwI GGATC 1 cut(s) 608
AoxI GGCC 3 cut(s) 116, 196, 903
ApeKI GCWGC 4 cut(s) 638, 743, 807, 894
ApoI RAATTY 3 cut(s) 134, 230, 658
AseI ATTAAT 1 cut(s) 417
AsiGI ACCGGT 1 cut(s) 149
AspLEI GCGC 1 cut(s) 802
AspS9I GGNCC 2 cut(s) 117, 871
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 5 cut(s) 98, 218, 478, 671, 817
AvaII GGWCC 1 cut(s) 871
BanI GGYRCC 1 cut(s) 620
BanII GRGCYC 1 cut(s) 592
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 4 cut(s) 650, 730, 819, 881
BccI CCATC 2 cut(s) 452, 671
BceAI ACGGC 2 cut(s) 115, 847
BciT130I CCWGG 1 cut(s) 121
BciVI GTATCC 1 cut(s) 85
BcnI CCSGG 1 cut(s) 618
BcoDI GTCTC 2 cut(s) 433, 881
BfaI CTAG 2 cut(s) 26, 861
BfmI CTRYAG 1 cut(s) 403
BfuI GTATCC 1 cut(s) 85
BisI GCNGC 6 cut(s) 199, 639, 744, 808, 895, 903
BlpI GCTNAGC 1 cut(s) 803
BlsI GCNGC 6 cut(s) 200, 640, 745, 809, 896, 904
BmcAI AGTACT 1 cut(s) 281
Bme1390I CCNGG 2 cut(s) 121, 618
Bme18I GGWCC 1 cut(s) 871
BmgT120I GGNCC 2 cut(s) 117, 871
BmiI GGNNCC 1 cut(s) 622
BmrFI CCNGG 2 cut(s) 121, 618
BpmI CTGGAG 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 545
Bpu1102I GCTNAGC 1 cut(s) 803
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 618
BsaAI YACGTR 1 cut(s) 14
BsaJI CCNNGG 6 cut(s) 87, 119, 120, 336, 829, 874
BsaWI WCCGGW 1 cut(s) 149
Bse118I RCCGGY 2 cut(s) 149, 763
Bse3DI GCAATG 2 cut(s) 208, 255
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 6 cut(s) 87, 119, 120, 336, 829, 874
BseGI GGATG 2 cut(s) 601, 730
BseMI GCAATG 2 cut(s) 208, 255
BseRI GAGGAG 3 cut(s) 600, 894, 899
BseXI GCAGC 4 cut(s) 650, 730, 819, 881
BsgI GTGCAG 2 cut(s) 657, 729
Bsh1236I CGCG 1 cut(s) 902
BshFI GGCC 3 cut(s) 118, 198, 905
BshNI GGYRCC 1 cut(s) 620
BshTI ACCGGT 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 592
BsiSI CCGG 3 cut(s) 150, 618, 764
BslFI GGGAC 1 cut(s) 842
BsmAI GTCTC 2 cut(s) 433, 881
BsmFI GGGAC 1 cut(s) 842
BsnI GGCC 3 cut(s) 118, 198, 905
Bsp1286I GDGCHC 1 cut(s) 592
Bsp143I GATC 4 cut(s) 103, 162, 613, 841
Bsp1720I GCTNAGC 1 cut(s) 803
Bsp19I CCATGG 3 cut(s) 87, 336, 874
BspACI CCGC 6 cut(s) 199, 213, 448, 549, 866, 902
BspANI GGCC 3 cut(s) 118, 198, 905
BspFNI CGCG 1 cut(s) 902
BspLI GGNNCC 1 cut(s) 622
BspPI GGATC 1 cut(s) 608
BspT107I GGYRCC 1 cut(s) 620
BsrDI GCAATG 2 cut(s) 208, 255
BsrFI RCCGGY 2 cut(s) 149, 763
BssAI RCCGGY 2 cut(s) 149, 763
BssECI CCNNGG 6 cut(s) 87, 119, 120, 336, 829, 874
BssMI GATC 4 cut(s) 103, 162, 613, 841
BssT1I CCWWGG 3 cut(s) 87, 336, 874
Bst2UI CCWGG 1 cut(s) 121
Bst4CI ACNGT 4 cut(s) 143, 350, 373, 455
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 3 cut(s) 215, 551, 574
BstDEI CTNAG 2 cut(s) 545, 803
BstDSI CCRYGG 4 cut(s) 87, 336, 829, 874
BstF5I GGATG 2 cut(s) 601, 730
BstFNI CGCG 1 cut(s) 902
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 4 cut(s) 106, 165, 616, 844
BstMAI GTCTC 2 cut(s) 433, 881
BstMBI GATC 4 cut(s) 103, 162, 613, 841
BstMWI GCNNNNNNNGC 1 cut(s) 797
BstNI CCWGG 1 cut(s) 121
BstSCI CCNGG 2 cut(s) 119, 616
BstSFI CTRYAG 1 cut(s) 403
BstUI CGCG 1 cut(s) 902
BstV1I GCAGC 4 cut(s) 650, 730, 819, 881
BsuI GTATCC 1 cut(s) 85
BsuRI GGCC 3 cut(s) 118, 198, 905
BtgI CCRYGG 4 cut(s) 87, 336, 829, 874
BtsCI GGATG 2 cut(s) 601, 730
BtsI GCAGTG 1 cut(s) 520
BtsIMutI CAGTG 2 cut(s) 369, 520
Cac8I GCNNGC 3 cut(s) 215, 551, 574
CfoI GCGC 1 cut(s) 802
Cfr10I RCCGGY 2 cut(s) 149, 763
Cfr13I GGNCC 2 cut(s) 117, 871
Csp6I GTAC 3 cut(s) 280, 514, 541
CspAI ACCGGT 1 cut(s) 149
CviAII CATG 4 cut(s) 69, 88, 337, 875
CviQI GTAC 3 cut(s) 280, 514, 541
DdeI CTNAG 2 cut(s) 545, 803
DpnI GATC 4 cut(s) 105, 164, 615, 843
DpnII GATC 4 cut(s) 103, 162, 613, 841
EaeI YGGCCR 2 cut(s) 196, 903
Ecl136II GAGCTC 1 cut(s) 590
Eco130I CCWWGG 3 cut(s) 87, 336, 874
Eco24I GRGCYC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 871
Eco53kI GAGCTC 1 cut(s) 590
Eco57I CTGAAG 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 590
EcoRI GAATTC 2 cut(s) 134, 658
EcoRII CCWGG 1 cut(s) 119
EcoT14I CCWWGG 3 cut(s) 87, 336, 874
EcoT38I GRGCYC 1 cut(s) 592
ErhI CCWWGG 3 cut(s) 87, 336, 874
FaeI CATG 4 cut(s) 72, 91, 340, 878
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FaqI GGGAC 1 cut(s) 842
FatI CATG 4 cut(s) 68, 87, 336, 874
FauI CCCGC 2 cut(s) 206, 542
Fnu4HI GCNGC 6 cut(s) 199, 639, 744, 808, 895, 903
FokI GGATG 2 cut(s) 588, 717
FriOI GRGCYC 1 cut(s) 592
Fsp4HI GCNGC 6 cut(s) 199, 639, 744, 808, 895, 903
FspBI CTAG 2 cut(s) 26, 861
GlaI GCGC 1 cut(s) 801
GluI GCNGC 6 cut(s) 199, 639, 744, 808, 895, 903
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 3 cut(s) 118, 198, 905
HapII CCGG 3 cut(s) 150, 618, 764
HhaI GCGC 1 cut(s) 802
Hin1II CATG 4 cut(s) 72, 91, 340, 878
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HindIII AAGCTT 2 cut(s) 191, 270
HinfI GANTC 2 cut(s) 111, 441
HpaII CCGG 3 cut(s) 150, 618, 764
HphI GGTGA 5 cut(s) 98, 218, 478, 671, 817
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 5 cut(s) 147, 229, 309, 613, 923
Hpy188III TCNNGA 2 cut(s) 299, 436
Hpy8I GTNNAC 1 cut(s) 541
HpyAV CCTTC 1 cut(s) 499
HpyCH4III ACNGT 4 cut(s) 143, 350, 373, 455
HpyCH4IV ACGT 2 cut(s) 13, 567
HpyCH4V TGCA 7 cut(s) 45, 188, 266, 353, 638, 746, 897
HpyF10VI GCNNNNNNNGC 1 cut(s) 797
HpyF3I CTNAG 2 cut(s) 545, 803
HpySE526I ACGT 2 cut(s) 13, 567
Hsp92II CATG 4 cut(s) 72, 91, 340, 878
HspAI GCGC 1 cut(s) 800
Kzo9I GATC 4 cut(s) 103, 162, 613, 841
LmnI GCTCC 2 cut(s) 371, 587
Lsp1109I GCAGC 4 cut(s) 650, 730, 819, 881
MaeI CTAG 2 cut(s) 26, 861
MaeII ACGT 2 cut(s) 13, 567
MaeIII GTNAC 4 cut(s) 152, 206, 340, 823
MalI GATC 4 cut(s) 105, 164, 615, 843
MboI GATC 4 cut(s) 103, 162, 613, 841
MboII GAAGA 1 cut(s) 17
MfeI CAATTG 1 cut(s) 752
MhlI GDGCHC 1 cut(s) 592
MluCI AATT 7 cut(s) 40, 134, 179, 230, 658, 752, 815
MlyI GAGTC 1 cut(s) 435
MseI TTAA 1 cut(s) 417
MspA1I CMGCKG 3 cut(s) 213, 408, 868
MspI CCGG 3 cut(s) 150, 618, 764
MspR9I CCNGG 2 cut(s) 121, 618
MunI CAATTG 1 cut(s) 752
MvaI CCWGG 1 cut(s) 121
MvnI CGCG 1 cut(s) 902
MwoI GCNNNNNNNGC 1 cut(s) 797
NciI CCSGG 1 cut(s) 618
NcoI CCATGG 3 cut(s) 87, 336, 874
NdeII GATC 4 cut(s) 103, 162, 613, 841
NlaIII CATG 4 cut(s) 72, 91, 340, 878
NlaIV GGNNCC 1 cut(s) 622
NmeAIII GCCGAG 1 cut(s) 551
NmuCI GTSAC 2 cut(s) 206, 823
PasI CCCWGGG 1 cut(s) 120
PfeI GAWTC 1 cut(s) 111
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 6 cut(s) 200, 640, 745, 809, 896, 904
PleI GAGTC 1 cut(s) 435
PpsI GAGTC 1 cut(s) 435
Ppu21I YACGTR 1 cut(s) 14
PshBI ATTAAT 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 592
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 1 cut(s) 622
PspPI GGNCC 2 cut(s) 117, 871
PvuII CAGCTG 1 cut(s) 408
RsaI GTAC 3 cut(s) 281, 515, 542
RsaNI GTAC 3 cut(s) 280, 514, 541
SacI GAGCTC 1 cut(s) 592
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 6 cut(s) 199, 639, 744, 808, 895, 903
Sau3AI GATC 4 cut(s) 103, 162, 613, 841
Sau96I GGNCC 2 cut(s) 117, 871
ScaI AGTACT 1 cut(s) 281
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 2 cut(s) 121, 618
SduI GDGCHC 1 cut(s) 592
SfcI CTRYAG 1 cut(s) 403
SinI GGWCC 1 cut(s) 871
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 7 cut(s) 40, 134, 179, 230, 658, 752, 815
SsiI CCGC 6 cut(s) 199, 213, 448, 549, 866, 902
SspMI CTAG 2 cut(s) 26, 861
SstI GAGCTC 1 cut(s) 592
StyD4I CCNGG 2 cut(s) 119, 616
StyI CCWWGG 3 cut(s) 87, 336, 874
TaaI ACNGT 4 cut(s) 143, 350, 373, 455
TaiI ACGT 2 cut(s) 16, 570
TaqI TCGA 2 cut(s) 468, 786
TasI AATT 7 cut(s) 40, 134, 179, 230, 658, 752, 815
TatI WGTACW 1 cut(s) 279
TauI GCSGC 2 cut(s) 201, 905
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 2 cut(s) 376, 527
TseFI GTSAC 2 cut(s) 206, 823
TseI GCWGC 4 cut(s) 638, 743, 807, 894
Tsp45I GTSAC 2 cut(s) 206, 823
TspDTI ATGAA 2 cut(s) 147, 438
TspRI CASTG 2 cut(s) 376, 527
VpaK11BI GGWCC 1 cut(s) 871
VspI ATTAAT 1 cut(s) 417
XapI RAATTY 3 cut(s) 134, 230, 658
XspI CTAG 2 cut(s) 26, 861
ZrmI AGTACT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.