Rh4CG066000

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000286
Physical Location & Seq
Forward (+)
12490660 .. 12492584
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG066000.1

Sequence Viewer

Length: 795 bp
ATGGAAGAAATACGTGAGGCTGCTTTAGCTTACTACCATAACTTGCCAGAGGACCTACAGAAGCAGGCATCTGCAAAATTCAAAGAAATTGATTCAAATGGTAATGGCACAATAAGCATCAGGGAATTCAAGAAGAGTATGGGAAGCTCTTTCGACAATGACTCCGCTATCATGAAGAGAAGCTTCAAGGAGCTGGACAAGAACGGAGACGGCAAGTTGGATTTCAACGAATACATCACTCTTTACTATCTTGTAGAGAGTGGTAGGGTGCTGATTTATTGTGCGGGCAATGGATGTGAGGCCGCACCTTTTCTCAAGGGACTCTATTTTACTTGTGTCGACTGCTTCCACCATAACGAAAATGAAACTTTTGATCTCTGCACCTCCTGCTACCGCAATGCTGACTTTGTTCATGAACACACCAACTTTGTGGATAACCATGTGTTACTTCGTTCCAAGGCTGCTTGTTGTGAATCCACATCTGAGCCATGCTCTTCTGGGTCTGATTCTGAGTCCAATCAAGTTAGTCGATCTGGATCAAAGAGAGTGCAAGTTAAGCGATCTGGATCAAAGAGGGTGAGTTTGTTTTCTTCACAAGCAGTCAACTATACTTCTTTGCATTTCAAAATTGTTTTCAGTATACTTCTTTGCCTTTTAATTAAGGTATGTAGTATTATCACGATGAATTTGACTTCAGCTAAACACATCCTGCTAGATGAACAAACACTATATAATACAAGACAAAAACATTGGTGCGTGACATCTCATCTTAAATTTCTAATTGGAGTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

264

Amino Acids

29.8

Weight (kDa)

7.1

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 25 - 83 3e-11 EF-hand domain pair
EF-hand_1 PF00036 58 - 81 9.1e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 339, 640
AciI CCGC 4 cut(s) 165, 284, 303, 394
AclWI GGATC 2 cut(s) 544, 574
AcsI RAATTY 4 cut(s) 77, 125, 685, 773
AcuI CTGAAG 1 cut(s) 678
AgsI TTSAA 6 cut(s) 82, 96, 130, 187, 226, 625
AluBI AGCT 5 cut(s) 29, 147, 183, 193, 698
AluI AGCT 5 cut(s) 29, 147, 183, 193, 698
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 2 cut(s) 544, 574
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 2 cut(s) 20, 461
ApoI RAATTY 4 cut(s) 77, 125, 685, 773
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 589
AvaII GGWCC 1 cut(s) 52
BbvI GCAGC 2 cut(s) 7, 448
BceAI ACGGC 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 1 cut(s) 713
BfmI CTRYAG 1 cut(s) 56
BisI GCNGC 3 cut(s) 21, 303, 462
BlsI GCNGC 3 cut(s) 22, 304, 463
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmsI GCATC 2 cut(s) 77, 126
BplI GAGNNNNNCTC 2 cut(s) 476, 508
BpuEI CTTGAG 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 14
BsaBI GATNNNNATC 2 cut(s) 535, 565
BsaJI CCNNGG 1 cut(s) 456
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bse3DI GCAATG 2 cut(s) 295, 403
Bse8I GATNNNNATC 2 cut(s) 535, 565
BseDI CCNNGG 1 cut(s) 456
BseGI GGATG 2 cut(s) 299, 705
BseJI GATNNNNATC 2 cut(s) 535, 565
BseMI GCAATG 2 cut(s) 295, 403
BseMII CTCAG 2 cut(s) 474, 501
BseXI GCAGC 2 cut(s) 7, 448
BsgI GTGCAG 1 cut(s) 364
BshFI GGCC 1 cut(s) 302
BslFI GGGAC 1 cut(s) 333
BsmAI GTCTC 1 cut(s) 201
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 333
BsnI GGCC 1 cut(s) 302
Bsp143I GATC 5 cut(s) 373, 530, 536, 560, 566
BspACI CCGC 4 cut(s) 165, 284, 303, 394
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 2 cut(s) 475, 502
BspHI TCATGA 2 cut(s) 171, 412
BspPI GGATC 2 cut(s) 544, 574
BspQI GCTCTTC 1 cut(s) 499
BsrDI GCAATG 2 cut(s) 295, 403
BssECI CCNNGG 1 cut(s) 456
BssMI GATC 5 cut(s) 373, 530, 536, 560, 566
BssNAI GTATAC 1 cut(s) 641
BssT1I CCWWGG 1 cut(s) 456
Bst1107I GTATAC 1 cut(s) 641
Bst6I CTCTTC 3 cut(s) 128, 170, 499
BstAPI GCANNNNNTGC 1 cut(s) 387
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 66, 286
BstDEI CTNAG 2 cut(s) 483, 510
BstF5I GGATG 2 cut(s) 299, 705
BstKTI GATC 5 cut(s) 376, 533, 539, 563, 569
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 5 cut(s) 373, 530, 536, 560, 566
BstMWI GCNNNNNNNGC 4 cut(s) 26, 114, 387, 556
BstSFI CTRYAG 1 cut(s) 56
BstV1I GCAGC 2 cut(s) 7, 448
BstXI CCANNNNNNTGG 1 cut(s) 430
BstZ17I GTATAC 1 cut(s) 641
BsuRI GGCC 1 cut(s) 302
BtsCI GGATG 2 cut(s) 299, 705
Cac8I GCNNGC 2 cut(s) 66, 286
CciI TCATGA 2 cut(s) 171, 412
Cfr13I GGNCC 1 cut(s) 52
CviAII CATG 4 cut(s) 172, 413, 440, 489
CviJI RGCY 9 cut(s) 20, 29, 147, 183, 193, 302, 461, 487, 698
CviKI_1 RGCY 9 cut(s) 20, 29, 147, 183, 193, 302, 461, 487, 698
DdeI CTNAG 2 cut(s) 483, 510
DpnI GATC 5 cut(s) 375, 532, 538, 562, 568
DpnII GATC 5 cut(s) 373, 530, 536, 560, 566
Eam1104I CTCTTC 3 cut(s) 128, 170, 499
EarI CTCTTC 3 cut(s) 128, 170, 499
Eco130I CCWWGG 1 cut(s) 456
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 456
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 4 cut(s) 175, 416, 443, 492
FalI AAGNNNNNCTT 2 cut(s) 167, 199
FaqI GGGAC 1 cut(s) 333
FatI CATG 4 cut(s) 171, 412, 439, 488
FauI CCCGC 1 cut(s) 277
FblI GTMKAC 2 cut(s) 339, 640
Fnu4HI GCNGC 3 cut(s) 21, 303, 462
FokI GGATG 2 cut(s) 306, 692
Fsp4HI GCNGC 3 cut(s) 21, 303, 462
FspBI CTAG 1 cut(s) 713
GluI GCNGC 3 cut(s) 21, 303, 462
HaeIII GGCC 1 cut(s) 302
Hin1II CATG 4 cut(s) 175, 416, 443, 492
HincII GTYRAC 2 cut(s) 340, 604
HindII GTYRAC 2 cut(s) 340, 604
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 6 cut(s) 92, 161, 321, 473, 506, 512
HphI GGTGA 1 cut(s) 589
Hpy166II GTNNAC 3 cut(s) 340, 604, 641
Hpy188I TCNGA 3 cut(s) 484, 505, 511
Hpy188III TCNNGA 6 cut(s) 130, 172, 413, 534, 564, 679
Hpy8I GTNNAC 3 cut(s) 340, 604, 641
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 4 cut(s) 74, 381, 550, 619
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 114, 387, 556
HpyF3I CTNAG 2 cut(s) 483, 510
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 4 cut(s) 175, 416, 443, 492
Kzo9I GATC 5 cut(s) 373, 530, 536, 560, 566
LguI GCTCTTC 1 cut(s) 499
LmnI GCTCC 1 cut(s) 190
LpnPI CCDG 9 cut(s) 50, 60, 106, 179, 400, 483, 519, 549, 722
Lsp1109I GCAGC 2 cut(s) 7, 448
LweI GCATC 2 cut(s) 77, 126
MaeI CTAG 1 cut(s) 713
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 2 cut(s) 444, 757
MalI GATC 5 cut(s) 375, 532, 538, 562, 568
MboI GATC 5 cut(s) 373, 530, 536, 560, 566
MboII GAAGA 5 cut(s) 17, 145, 187, 486, 582
MluCI AATT 9 cut(s) 77, 87, 125, 627, 657, 685, 773, 780, 790
MlyI GAGTC 3 cut(s) 155, 315, 521
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 5 cut(s) 10, 43, 292, 394, 567
MseI TTAA 5 cut(s) 555, 656, 660, 771, 789
MwoI GCNNNNNNNGC 4 cut(s) 26, 114, 387, 556
NdeII GATC 5 cut(s) 373, 530, 536, 560, 566
NlaIII CATG 4 cut(s) 175, 416, 443, 492
NmuCI GTSAC 1 cut(s) 757
PacI TTAATTAA 1 cut(s) 660
PagI TCATGA 2 cut(s) 171, 412
PciSI GCTCTTC 1 cut(s) 499
PfeI GAWTC 3 cut(s) 92, 473, 506
PkrI GCNGC 3 cut(s) 22, 304, 463
PleI GAGTC 3 cut(s) 155, 315, 520
PpsI GAGTC 3 cut(s) 155, 315, 520
Ppu21I YACGTR 1 cut(s) 14
PpuMI RGGWCCY 1 cut(s) 52
Psp5II RGGWCCY 1 cut(s) 52
PspPI GGNCC 1 cut(s) 52
PspPPI RGGWCCY 1 cut(s) 52
SalI GTCGAC 1 cut(s) 338
SapI GCTCTTC 1 cut(s) 499
SaqAI TTAA 5 cut(s) 555, 656, 660, 771, 789
SatI GCNGC 3 cut(s) 21, 303, 462
Sau3AI GATC 5 cut(s) 373, 530, 536, 560, 566
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 3 cut(s) 155, 315, 521
SfaNI GCATC 2 cut(s) 77, 126
SfcI CTRYAG 1 cut(s) 56
SinI GGWCC 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 9 cut(s) 77, 87, 125, 627, 657, 685, 773, 780, 790
SsiI CCGC 4 cut(s) 165, 284, 303, 394
SspMI CTAG 1 cut(s) 713
StyI CCWWGG 1 cut(s) 456
TaiI ACGT 1 cut(s) 16
TaqI TCGA 3 cut(s) 153, 339, 529
TasI AATT 9 cut(s) 77, 87, 125, 627, 657, 685, 773, 780, 790
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 3 cut(s) 92, 473, 506
Tru1I TTAA 5 cut(s) 555, 656, 660, 771, 789
Tru9I TTAA 5 cut(s) 555, 656, 660, 771, 789
TseFI GTSAC 1 cut(s) 757
TseI GCWGC 2 cut(s) 20, 461
Tsp45I GTSAC 1 cut(s) 757
TspDTI ATGAA 6 cut(s) 188, 378, 401, 429, 698, 732
TspGWI ACGGA 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 52
XapI RAATTY 4 cut(s) 77, 125, 685, 773
XmiI GTMKAC 2 cut(s) 339, 640
XspI CTAG 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.