FvH4_4g04900

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000286
Physical Location & Seq
Forward (+)
4244000 .. 4249229
5230 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04900.t1

Sequence Viewer

Length: 996 bp
ATGGGAGACATGAAAGAAATTCGTGATGCTGCTTTAGCTTATTACTATAATCTGTCTAAAGAGCAACAGCATGAGGTGTATACCTTTTTTCAAGCAATGGATAAAGATGGCGACGGAGCGATAACCATCGAGGAGTTCGAGCAGATTCTCACTCTCTTTTCCCGCGACTATGTCAATCGCTTGTTCCGGGCGATAGATAAAAATGGCGATGGTGTTTTGGATTTTGAGGAAGCTATCACCTTTTACTATGTTCTCTGTACTTATAGGATGGTCAATTGCGATGGATGCAAAGATTCTCTTCTAACGGGACTGTACTTCACTTGTGTCGACTGCTTCGATCATAACCAAACTACCACTTTTGATCTTTGCATCTCCTGCTACCGCACCCGCAACTACGTTCACGAACACTGCAACTTTGTGGACAACTATGCCTTGCTGCGTACCAGGGCTGCTTATATCAATCACGTATCAACAGACCAGGTCCAGCGAACAAGTTCAGAAAAGTCGGTTGATGACTGTCAAGTTCCTCGATCAAAATCTAAAAAAGTGTATGCCTTTTTTCGAGCAATGGATAAAGATGGCGACGGATCAATAACCATCAAGGAGTTCGAGGAGATTCTCACCATCTCTTCCCGCCCCAATGCCAATCGCTTGTTCCGGGCGATGGATAAAAATGGCGATGGTGTTTTGGATTTTGAGGAAGCTATAACCTTTTACTATGTTCTCTGTAATTATAGGATGGTTGGTTGCGACGGATGCAAAGATTCTCTTCTAACGGGACTGTACTTCACTTGTATCGACTGCTTCGATCATAACCAAACTACCAGTTTTGATCTTTGCATCTCCTGCTACCGCACCCGCAACTACGTTCACCAACACTGCAACTTTTTGGACAACTGTGCCATACTGCGTACCGAGGCTGCTTATATCAATCACGTATTAACAGACCAGGTCCAGGTGATTTATCAATTCAACTCTTTTGCATGGATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

332

Amino Acids

38.5

Weight (kDa)

4.99

Isoelectric Point (pI)

30.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 26 - 51 2.6e-06 EF hand domain
EF-hand_7 PF13499 27 - 82 1.6e-10 EF-hand domain pair
EF-hand_5 PF13202 29 - 49 1.3e-06 EF hand
EF-hand_7 PF13499 182 - 239 1.7e-09 EF-hand domain pair
EF-hand_1 PF00036 185 - 208 4.9e-06 EF hand domain
EF-hand_5 PF13202 185 - 206 7.3e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 80, 327
AccII CGCG 1 cut(s) 165
AciI CCGC 6 cut(s) 163, 382, 388, 634, 853, 859
AclWI GGATC 1 cut(s) 595
AcsI RAATTY 1 cut(s) 18
AfaI GTAC 5 cut(s) 259, 314, 442, 785, 913
AfiI CCNNNNNNNGG 2 cut(s) 664, 955
AgsI TTSAA 2 cut(s) 92, 973
AjnI CCWGG 4 cut(s) 443, 477, 948, 954
AjuI GAANNNNNNNTTGG 2 cut(s) 638, 670
AluBI AGCT 3 cut(s) 38, 233, 704
AluI AGCT 3 cut(s) 38, 233, 704
AlwI GGATC 1 cut(s) 595
ApeKI GCWGC 4 cut(s) 29, 436, 449, 920
ApoI RAATTY 1 cut(s) 18
AseI ATTAAT 1 cut(s) 990
Asp700I GAANNNNTTC 1 cut(s) 493
AspS9I GGNCC 2 cut(s) 481, 952
AsuC2I CCSGG 2 cut(s) 188, 659
AsuHPI GGTGA 4 cut(s) 229, 613, 863, 970
AvaII GGWCC 2 cut(s) 481, 952
BbvI GCAGC 4 cut(s) 16, 423, 436, 907
BciT130I CCWGG 4 cut(s) 445, 479, 950, 956
BcnI CCSGG 2 cut(s) 188, 659
BisI GCNGC 4 cut(s) 30, 437, 450, 921
BlsI GCNGC 4 cut(s) 31, 438, 451, 922
Bme1390I CCNGG 6 cut(s) 188, 445, 479, 659, 950, 956
Bme18I GGWCC 2 cut(s) 481, 952
BmgT120I GGNCC 2 cut(s) 481, 952
BmrFI CCNGG 6 cut(s) 188, 445, 479, 659, 950, 956
BmsI GCATC 5 cut(s) 16, 275, 378, 746, 849
BpuMI CCSGG 2 cut(s) 188, 659
BsaAI YACGTR 2 cut(s) 466, 937
BsaBI GATNNNNATC 2 cut(s) 125, 535
BsaJI CCNNGG 2 cut(s) 444, 915
Bsc4I CCNNNNNNNGG 2 cut(s) 664, 955
Bse1I ACTGG 1 cut(s) 825
Bse3DI GCAATG 2 cut(s) 102, 573
Bse8I GATNNNNATC 2 cut(s) 125, 535
BseBI CCWGG 4 cut(s) 445, 479, 950, 956
BseDI CCNNGG 2 cut(s) 444, 915
BseGI GGATG 4 cut(s) 273, 290, 744, 761
BseJI GATNNNNATC 2 cut(s) 125, 535
BseLI CCNNNNNNNGG 2 cut(s) 664, 955
BseMI GCAATG 2 cut(s) 102, 573
BseNI ACTGG 1 cut(s) 825
BseRI GAGGAG 2 cut(s) 146, 626
BseXI GCAGC 4 cut(s) 16, 423, 436, 907
Bsh1236I CGCG 1 cut(s) 165
BsiSI CCGG 2 cut(s) 187, 658
BslFI GGGAC 2 cut(s) 321, 792
BslI CCNNNNNNNGG 2 cut(s) 664, 955
BsmFI GGGAC 2 cut(s) 321, 792
Bsp143I GATC 6 cut(s) 337, 361, 530, 587, 808, 832
BspACI CCGC 6 cut(s) 163, 382, 388, 634, 853, 859
BspFNI CGCG 1 cut(s) 165
BspPI GGATC 1 cut(s) 595
BsrDI GCAATG 2 cut(s) 102, 573
BsrI ACTGG 1 cut(s) 825
BssECI CCNNGG 2 cut(s) 444, 915
BssMI GATC 6 cut(s) 337, 361, 530, 587, 808, 832
BssNAI GTATAC 1 cut(s) 81
Bst1107I GTATAC 1 cut(s) 81
Bst2UI CCWGG 4 cut(s) 445, 479, 950, 956
Bst4CI ACNGT 4 cut(s) 312, 518, 783, 899
Bst6I CTCTTC 3 cut(s) 303, 634, 774
BstAPI GCANNNNNTGC 2 cut(s) 375, 846
BstBAI YACGTR 2 cut(s) 466, 937
BstF5I GGATG 4 cut(s) 273, 290, 744, 761
BstFNI CGCG 1 cut(s) 165
BstKTI GATC 6 cut(s) 340, 364, 533, 590, 811, 835
BstMBI GATC 6 cut(s) 337, 361, 530, 587, 808, 832
BstMWI GCNNNNNNNGC 5 cut(s) 35, 285, 375, 756, 846
BstNI CCWGG 4 cut(s) 445, 479, 950, 956
BstSCI CCNGG 6 cut(s) 186, 443, 477, 657, 948, 954
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 4 cut(s) 16, 423, 436, 907
BstZ17I GTATAC 1 cut(s) 81
BtgZI GCGATG 4 cut(s) 222, 294, 677, 693
BtsCI GGATG 4 cut(s) 273, 290, 744, 761
BtsI GCAGTG 2 cut(s) 406, 877
BtsIMutI CAGTG 2 cut(s) 406, 877
Cfr13I GGNCC 2 cut(s) 481, 952
CsiI ACCWGGT 2 cut(s) 477, 948
Csp6I GTAC 5 cut(s) 258, 313, 441, 784, 912
CviAII CATG 3 cut(s) 10, 71, 984
CviJI RGCY 5 cut(s) 38, 233, 449, 704, 920
CviKI_1 RGCY 5 cut(s) 38, 233, 449, 704, 920
CviQI GTAC 5 cut(s) 258, 313, 441, 784, 912
DpnI GATC 6 cut(s) 339, 363, 532, 589, 810, 834
DpnII GATC 6 cut(s) 337, 361, 530, 587, 808, 832
Eam1104I CTCTTC 3 cut(s) 303, 634, 774
EarI CTCTTC 3 cut(s) 303, 634, 774
Eco47I GGWCC 2 cut(s) 481, 952
EcoRII CCWGG 4 cut(s) 443, 477, 948, 954
FaeI CATG 3 cut(s) 13, 74, 987
FalI AAGNNNNNCTT 4 cut(s) 282, 314, 753, 785
FaqI GGGAC 2 cut(s) 321, 792
FatI CATG 3 cut(s) 9, 70, 983
FauI CCCGC 4 cut(s) 170, 395, 641, 866
FblI GTMKAC 2 cut(s) 80, 327
Fnu4HI GCNGC 4 cut(s) 30, 437, 450, 921
FokI GGATG 4 cut(s) 280, 297, 751, 768
Fsp4HI GCNGC 4 cut(s) 30, 437, 450, 921
GluI GCNGC 4 cut(s) 30, 437, 450, 921
HapII CCGG 2 cut(s) 187, 658
Hin1II CATG 3 cut(s) 13, 74, 987
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 4 cut(s) 145, 293, 616, 764
HpaII CCGG 2 cut(s) 187, 658
HphI GGTGA 4 cut(s) 229, 613, 863, 970
Hpy166II GTNNAC 5 cut(s) 81, 328, 400, 421, 871
Hpy188I TCNGA 1 cut(s) 499
Hpy188III TCNNGA 2 cut(s) 23, 401
Hpy8I GTNNAC 5 cut(s) 81, 328, 400, 421, 871
Hpy99I CGWCG 3 cut(s) 116, 587, 755
HpyCH4III ACNGT 4 cut(s) 312, 518, 783, 899
HpyCH4IV ACGT 4 cut(s) 396, 465, 867, 936
HpyCH4V TGCA 7 cut(s) 288, 369, 411, 759, 840, 882, 983
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 285, 375, 756, 846
HpySE526I ACGT 4 cut(s) 396, 465, 867, 936
Hsp92II CATG 3 cut(s) 13, 74, 987
Kzo9I GATC 6 cut(s) 337, 361, 530, 587, 808, 832
LmnI GCTCC 1 cut(s) 116
Lsp1109I GCAGC 4 cut(s) 16, 423, 436, 907
LweI GCATC 5 cut(s) 16, 275, 378, 746, 849
MabI ACCWGGT 2 cut(s) 477, 948
MaeII ACGT 4 cut(s) 396, 465, 867, 936
MalI GATC 6 cut(s) 339, 363, 532, 589, 810, 834
MboI GATC 6 cut(s) 337, 361, 530, 587, 808, 832
MboII GAAGA 3 cut(s) 290, 621, 761
MfeI CAATTG 1 cut(s) 274
MluCI AATT 5 cut(s) 18, 274, 730, 968, 991
MnlI CCTC 7 cut(s) 67, 124, 220, 537, 604, 691, 910
MroXI GAANNNNTTC 1 cut(s) 493
MseI TTAA 3 cut(s) 941, 990, 994
MspI CCGG 2 cut(s) 187, 658
MspR9I CCNGG 6 cut(s) 188, 445, 479, 659, 950, 956
MunI CAATTG 1 cut(s) 274
MvaI CCWGG 4 cut(s) 445, 479, 950, 956
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 5 cut(s) 35, 285, 375, 756, 846
NciI CCSGG 2 cut(s) 188, 659
NdeII GATC 6 cut(s) 337, 361, 530, 587, 808, 832
NlaIII CATG 3 cut(s) 13, 74, 987
PacI TTAATTAA 1 cut(s) 994
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 333, 804
PdmI GAANNNNTTC 1 cut(s) 493
PfeI GAWTC 4 cut(s) 145, 293, 616, 764
PflFI GACNNNGTC 3 cut(s) 170, 479, 950
PkrI GCNGC 4 cut(s) 31, 438, 451, 922
Ppu21I YACGTR 2 cut(s) 466, 937
PshBI ATTAAT 1 cut(s) 990
Psp6I CCWGG 4 cut(s) 443, 477, 948, 954
PspGI CCWGG 4 cut(s) 443, 477, 948, 954
PspPI GGNCC 2 cut(s) 481, 952
PsyI GACNNNGTC 3 cut(s) 170, 479, 950
RsaI GTAC 5 cut(s) 259, 314, 442, 785, 913
RsaNI GTAC 5 cut(s) 258, 313, 441, 784, 912
SalI GTCGAC 1 cut(s) 326
SaqAI TTAA 3 cut(s) 941, 990, 994
SatI GCNGC 4 cut(s) 30, 437, 450, 921
Sau3AI GATC 6 cut(s) 337, 361, 530, 587, 808, 832
Sau96I GGNCC 2 cut(s) 481, 952
ScrFI CCNGG 6 cut(s) 188, 445, 479, 659, 950, 956
SexAI ACCWGGT 2 cut(s) 477, 948
SfaNI GCATC 5 cut(s) 16, 275, 378, 746, 849
SinI GGWCC 2 cut(s) 481, 952
Sse9I AATT 5 cut(s) 18, 274, 730, 968, 991
SsiI CCGC 6 cut(s) 163, 382, 388, 634, 853, 859
StyD4I CCNGG 6 cut(s) 186, 443, 477, 657, 948, 954
TaaI ACNGT 4 cut(s) 312, 518, 783, 899
TaiI ACGT 4 cut(s) 399, 468, 870, 939
TaqI TCGA 9 cut(s) 129, 138, 327, 336, 529, 562, 609, 798, 807
TasI AATT 5 cut(s) 18, 274, 730, 968, 991
TatI WGTACW 3 cut(s) 257, 312, 783
TfiI GAWTC 4 cut(s) 145, 293, 616, 764
Tru1I TTAA 3 cut(s) 941, 990, 994
Tru9I TTAA 3 cut(s) 941, 990, 994
TscAI CASTG 2 cut(s) 413, 884
TseI GCWGC 4 cut(s) 29, 436, 449, 920
TspDTI ATGAA 1 cut(s) 26
TspGWI ACGGA 3 cut(s) 129, 600, 768
TspRI CASTG 2 cut(s) 413, 884
Tth111I GACNNNGTC 3 cut(s) 170, 479, 950
VpaK11BI GGWCC 2 cut(s) 481, 952
VspI ATTAAT 1 cut(s) 990
XapI RAATTY 1 cut(s) 18
XmiI GTMKAC 2 cut(s) 80, 327
XmnI GAANNNNTTC 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.