Rh4DG056200

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000286
Physical Location & Seq
Forward (+)
8699974 .. 8702764
2791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG056200.1

Sequence Viewer

Length: 582 bp
ATGGACATGCAAGAAATACGCAACGCTGCTTTAGCTTATTACCAGAACTTATCAGATGAGCTAAAGCAGAAGGCATCTGAAAAATTTAAAGAAATGGATTCGAATGATAATGGCACAATAAGCATCAAGGAGTTCCGGCGGAGTTTGGGCAGCTCCGAGATCAAGCGCTGCTTCAAGTTGCTGGACAAAACCGGAGACGGCAAGTTGGATTTCAACGAATTCATCACTCTTTACTATCTCGTGGAGAGTGGGAGAGGGGTTTTCTGTGATGGCCATGGATGTAAGGCCTCCCCCTTTCTCAACGGACTGTATTTTACTTGCGTTGAGTGCTTCCACAACAACGAAGCTGCAACGTTCGATCTCTGCACCTCCTGCTACCGCGATGGAAAGTATATTCACAACCATGCCAGCTTTTTGGATAACTACGTCTTGCTGCGTTCCAAGGCTGCTTGTGAATCCACAACACCAACGGAAGATGATCATCATGAGAGGGCAGTATTTGAAGCAATTAAATCACTCAATTCCCAAGTTAGCTTTGGAGATGCTGCTGCTGATGAGTCCAGTTCCTGCAGCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

193

Amino Acids

21.71

Weight (kDa)

5.13

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 23 - 79 8.1e-11 EF-hand domain pair
EF-hand_1 PF00036 53 - 77 3e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 381
AciI CCGC 2 cut(s) 139, 379
AclI AACGTT 1 cut(s) 353
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 2 cut(s) 83, 218
AfeI AGCGCT 1 cut(s) 167
AgsI TTSAA 3 cut(s) 175, 214, 503
AluBI AGCT 6 cut(s) 35, 61, 153, 347, 411, 534
AluI AGCT 6 cut(s) 35, 61, 153, 347, 411, 534
Alw26I GTCTC 1 cut(s) 189
AlwNI CAGNNNCTG 1 cut(s) 567
Aor51HI AGCGCT 1 cut(s) 167
AoxI GGCC 2 cut(s) 271, 285
ApeKI GCWGC 9 cut(s) 26, 150, 168, 347, 433, 446, 545, 548, 570
ApoI RAATTY 2 cut(s) 83, 218
AspLEI GCGC 1 cut(s) 168
AsuII TTCGAA 1 cut(s) 101
BalI TGGCCA 1 cut(s) 273
BauI CACGAG 1 cut(s) 239
BbvI GCAGC 8 cut(s) 13, 155, 162, 334, 420, 433, 532, 535
BccI CCATC 2 cut(s) 263, 377
BceAI ACGGC 1 cut(s) 214
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 568
BfoI RGCGCY 1 cut(s) 169
BisI GCNGC 9 cut(s) 27, 151, 169, 348, 434, 447, 546, 549, 571
BlsI GCNGC 9 cut(s) 28, 152, 170, 349, 435, 448, 547, 550, 572
BmsI GCATC 3 cut(s) 83, 132, 532
Bpu14I TTCGAA 1 cut(s) 101
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 2 cut(s) 274, 441
BsaWI WCCGGW 1 cut(s) 191
Bse1I ACTGG 1 cut(s) 561
Bse8I GATNNNNATC 1 cut(s) 480
BseDI CCNNGG 2 cut(s) 274, 441
BseGI GGATG 1 cut(s) 284
BseJI GATNNNNATC 1 cut(s) 480
BseNI ACTGG 1 cut(s) 561
BseXI GCAGC 8 cut(s) 13, 155, 162, 334, 420, 433, 532, 535
BsgI GTGCAG 1 cut(s) 349
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 2 cut(s) 273, 287
BsiSI CCGG 2 cut(s) 136, 192
BsmAI GTCTC 1 cut(s) 189
BsmBI CGTCTC 1 cut(s) 189
BsnI GGCC 2 cut(s) 273, 287
Bsp119I TTCGAA 1 cut(s) 101
Bsp143I GATC 3 cut(s) 159, 358, 478
Bsp19I CCATGG 1 cut(s) 274
BspACI CCGC 2 cut(s) 139, 379
BspANI GGCC 2 cut(s) 273, 287
BspFNI CGCG 1 cut(s) 381
BspHI TCATGA 1 cut(s) 484
BspMAI CTGCAG 1 cut(s) 572
BspT104I TTCGAA 1 cut(s) 101
BsrI ACTGG 1 cut(s) 561
BssECI CCNNGG 2 cut(s) 274, 441
BssMI GATC 3 cut(s) 159, 358, 478
BssSI CACGAG 1 cut(s) 239
BssT1I CCWWGG 2 cut(s) 274, 441
Bst2BI CACGAG 1 cut(s) 239
Bst4CI ACNGT 1 cut(s) 309
BstAPI GCANNNNNTGC 1 cut(s) 372
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 409
BstDSI CCRYGG 1 cut(s) 274
BstF5I GGATG 1 cut(s) 284
BstFNI CGCG 1 cut(s) 381
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 3 cut(s) 162, 361, 481
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 3 cut(s) 159, 358, 478
BstMWI GCNNNNNNNGC 4 cut(s) 32, 120, 327, 372
BstNSI RCATGY 1 cut(s) 10
BstSFI CTRYAG 1 cut(s) 568
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 8 cut(s) 13, 155, 162, 334, 420, 433, 532, 535
BstXI CCANNNNNNTGG 1 cut(s) 415
BsuRI GGCC 2 cut(s) 273, 287
BtgI CCRYGG 1 cut(s) 274
BtgZI GCGATG 1 cut(s) 396
BtsCI GGATG 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 409
CaiI CAGNNNCTG 1 cut(s) 567
CciI TCATGA 1 cut(s) 484
CfoI GCGC 1 cut(s) 168
CviAII CATG 4 cut(s) 7, 275, 404, 485
CviJI RGCY 9 cut(s) 35, 61, 153, 273, 287, 347, 411, 446, 534
CviKI_1 RGCY 9 cut(s) 35, 61, 153, 273, 287, 347, 411, 446, 534
DpnI GATC 3 cut(s) 161, 360, 480
DpnII GATC 3 cut(s) 159, 358, 478
DraI TTTAAA 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 271
EciI GGCGGA 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 274, 441
Eco147I AGGCCT 1 cut(s) 287
Eco47III AGCGCT 1 cut(s) 167
EcoRI GAATTC 1 cut(s) 218
EcoT14I CCWWGG 2 cut(s) 274, 441
ErhI CCWWGG 2 cut(s) 274, 441
Esp3I CGTCTC 1 cut(s) 189
FaeI CATG 4 cut(s) 10, 278, 407, 488
FaiI YATR 6 cut(s) 8, 276, 393, 405, 486, 578
FalI AAGNNNNNCTT 2 cut(s) 155, 187
FatI CATG 4 cut(s) 6, 274, 403, 484
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 9 cut(s) 27, 151, 169, 348, 434, 447, 546, 549, 571
FokI GGATG 1 cut(s) 291
Fsp4HI GCNGC 9 cut(s) 27, 151, 169, 348, 434, 447, 546, 549, 571
GlaI GCGC 1 cut(s) 167
GluI GCNGC 9 cut(s) 27, 151, 169, 348, 434, 447, 546, 549, 571
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 2 cut(s) 273, 287
HapII CCGG 2 cut(s) 136, 192
HhaI GCGC 1 cut(s) 168
Hin1II CATG 4 cut(s) 10, 278, 407, 488
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HinfI GANTC 3 cut(s) 98, 455, 557
HpaII CCGG 2 cut(s) 136, 192
Hpy188I TCNGA 3 cut(s) 55, 79, 157
Hpy188III TCNNGA 1 cut(s) 485
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 1 cut(s) 309
HpyCH4IV ACGT 2 cut(s) 353, 426
HpyCH4V TGCA 4 cut(s) 10, 350, 366, 570
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 120, 327, 372
HpySE526I ACGT 2 cut(s) 353, 426
Hsp92II CATG 4 cut(s) 10, 278, 407, 488
HspAI GCGC 1 cut(s) 166
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 3 cut(s) 159, 358, 478
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 7 cut(s) 56, 149, 167, 205, 385, 421, 574
Lsp1109I GCAGC 8 cut(s) 13, 155, 162, 334, 420, 433, 532, 535
LweI GCATC 3 cut(s) 83, 132, 532
MaeII ACGT 2 cut(s) 353, 426
MalI GATC 3 cut(s) 161, 360, 480
MboI GATC 3 cut(s) 159, 358, 478
MboII GAAGA 1 cut(s) 485
MlsI TGGCCA 1 cut(s) 273
MluCI AATT 4 cut(s) 83, 218, 507, 520
MluNI TGGCCA 1 cut(s) 273
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 1 cut(s) 186
MnlI CCTC 4 cut(s) 248, 298, 379, 483
Mox20I TGGCCA 1 cut(s) 273
MscI TGGCCA 1 cut(s) 273
MseI TTAA 2 cut(s) 87, 510
MslI CAYNNNNRTG 1 cut(s) 402
Msp20I TGGCCA 1 cut(s) 273
MspI CCGG 2 cut(s) 136, 192
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 4 cut(s) 32, 120, 327, 372
NcoI CCATGG 1 cut(s) 274
NdeII GATC 3 cut(s) 159, 358, 478
NlaIII CATG 4 cut(s) 10, 278, 407, 488
NspI RCATGY 1 cut(s) 10
NspV TTCGAA 1 cut(s) 101
PagI TCATGA 1 cut(s) 484
PceI AGGCCT 1 cut(s) 287
PfeI GAWTC 2 cut(s) 98, 455
PkrI GCNGC 9 cut(s) 28, 152, 170, 349, 435, 448, 547, 550, 572
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Psp1406I AACGTT 1 cut(s) 353
PstI CTGCAG 1 cut(s) 572
PstNI CAGNNNCTG 1 cut(s) 567
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 2 cut(s) 87, 510
SatI GCNGC 9 cut(s) 27, 151, 169, 348, 434, 447, 546, 549, 571
Sau3AI GATC 3 cut(s) 159, 358, 478
SchI GAGTC 1 cut(s) 566
SetI ASST 9 cut(s) 37, 63, 155, 349, 356, 371, 413, 429, 536
SfaNI GCATC 3 cut(s) 83, 132, 532
SfcI CTRYAG 1 cut(s) 568
SfuI TTCGAA 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 402
Sse9I AATT 4 cut(s) 83, 218, 507, 520
SseBI AGGCCT 1 cut(s) 287
SsiI CCGC 2 cut(s) 139, 379
StuI AGGCCT 1 cut(s) 287
StyI CCWWGG 2 cut(s) 274, 441
TaaI ACNGT 1 cut(s) 309
TaiI ACGT 2 cut(s) 356, 429
TaqI TCGA 2 cut(s) 101, 357
TasI AATT 4 cut(s) 83, 218, 507, 520
TfiI GAWTC 2 cut(s) 98, 455
Tru1I TTAA 2 cut(s) 87, 510
Tru9I TTAA 2 cut(s) 87, 510
TseI GCWGC 9 cut(s) 26, 150, 168, 347, 433, 446, 545, 548, 570
TspDTI ATGAA 1 cut(s) 211
TspGWI ACGGA 2 cut(s) 318, 485
XapI RAATTY 2 cut(s) 83, 218
XceI RCATGY 1 cut(s) 10
XcmI CCANNNNNNNNNTGG 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.