Rroxscaffold_5G00340760

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000286
Physical Location & Seq
Reverse (-)
9242256 .. 9243576
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00340760.1

Sequence Viewer

Length: 612 bp
ATGGAGGAGTTGCGACAAACGGCATTAGCATATTACCAGGATGCCCCAGAAGACATGCGGTCGGCAGTAGACCATTTTTTCCAGGAGATGGATCCTGATGCAAAGGACCGAGTGAGCCGGCAAGATTTCTTAGAATACATGAAGATGGATGAAGATTGCGCACATATGAGTAACCACAGTTTCTTTGATGTGCTGAAGACGGAAGAGAGTGAGGGGCTGGATTTTATGGACGTAAGCCAAGAGGAGGAAGCTCTGAGCTTGAAGCTGCAGCAAGAAGCATCAAGCATAATTAATGGGTCAAAGGCACATGAGTCCGTTGGAGCAAAAGATCTGAAAAAGAAGCTCCAGTCTAGCAGTGGCAGTAGAACAGTAACTTTAAGAGAAGCAAACGCTGCTTCTTCTCCTAAACCACCGACACCGGAGCCAAGCAGTCCCACATCCAATGCACCGGAGGGAAGTAATAGATCCACATCCAACGCTCTGGTTCCTGTGAATCCTAAGCCTAAACATAGAAGTAGTGCAGGGAAGAATGCAATTAAGGCGGTGGAGCTGGCACTTGCCCTCGGAAATATATTTGTCACCAGTACTGCGTCTCAGTGTGTCATTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5

Protein Analysis

203

Amino Acids

22.18

Weight (kDa)

5.08

Isoelectric Point (pI)

65.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 160
AccB7I CCANNNNNTGG 1 cut(s) 88
AccI GTMKAC 1 cut(s) 69
AciI CCGC 2 cut(s) 58, 542
AclWI GGATC 3 cut(s) 86, 99, 459
AcuI CTGAAG 1 cut(s) 215
AfaI GTAC 1 cut(s) 586
AfiI CCNNNNNNNGG 2 cut(s) 88, 244
AgsI TTSAA 1 cut(s) 262
AhdI GACNNNNNGTC 1 cut(s) 58
AjnI CCWGG 2 cut(s) 36, 81
AluBI AGCT 5 cut(s) 251, 258, 265, 343, 550
AluI AGCT 5 cut(s) 251, 258, 265, 343, 550
Alw26I GTCTC 1 cut(s) 597
AlwI GGATC 3 cut(s) 86, 99, 459
ApeKI GCWGC 3 cut(s) 265, 268, 392
AseI ATTAAT 1 cut(s) 291
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 1 cut(s) 571
AvaII GGWCC 1 cut(s) 106
BamHI GGATCC 1 cut(s) 91
BbsI GAAGAC 2 cut(s) 57, 203
BbvI GCAGC 3 cut(s) 252, 280, 379
BccI CCATC 2 cut(s) 82, 139
BceAI ACGGC 1 cut(s) 36
BciT130I CCWGG 2 cut(s) 38, 83
BcoDI GTCTC 1 cut(s) 597
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 266
BglII AGATCT 1 cut(s) 328
BisI GCNGC 3 cut(s) 266, 269, 393
BlsI GCNGC 3 cut(s) 267, 270, 394
BmcAI AGTACT 1 cut(s) 586
Bme1390I CCNGG 2 cut(s) 38, 83
Bme18I GGWCC 1 cut(s) 106
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 3 cut(s) 93, 423, 486
BmrFI CCNGG 2 cut(s) 38, 83
BmsI GCATC 3 cut(s) 31, 88, 287
BpiI GAAGAC 2 cut(s) 57, 203
BpmI CTGGAG 1 cut(s) 329
Bpu10I CCTNAGC 1 cut(s) 498
BsaBI GATNNNNATC 1 cut(s) 469
BsaJI CCNNGG 1 cut(s) 562
BsaWI WCCGGW 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 244
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 2 cut(s) 346, 582
Bse8I GATNNNNATC 1 cut(s) 469
BseBI CCWGG 2 cut(s) 38, 83
BseDI CCNNGG 1 cut(s) 562
BseGI GGATG 4 cut(s) 46, 154, 437, 470
BseJI GATNNNNATC 1 cut(s) 469
BseLI CCNNNNNNNGG 2 cut(s) 88, 244
BseMII CTCAG 2 cut(s) 245, 608
BseNI ACTGG 2 cut(s) 346, 582
BseRI GAGGAG 2 cut(s) 20, 257
BseXI GCAGC 3 cut(s) 252, 280, 379
BsgI GTGCAG 1 cut(s) 540
Bsh1285I CGRYCG 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 62
BsiSI CCGG 3 cut(s) 118, 419, 449
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 2 cut(s) 88, 244
BsmAI GTCTC 1 cut(s) 597
BsmBI CGTCTC 1 cut(s) 597
BsmFI GGGAC 1 cut(s) 417
BsmI GAATGC 1 cut(s) 535
Bsp143I GATC 3 cut(s) 91, 328, 464
BspACI CCGC 2 cut(s) 58, 542
BspCNI CTCAG 2 cut(s) 246, 607
BspLI GGNNCC 3 cut(s) 93, 423, 486
BspMAI CTGCAG 1 cut(s) 270
BspPI GGATC 3 cut(s) 86, 99, 459
BsrFI RCCGGY 1 cut(s) 117
BsrI ACTGG 2 cut(s) 346, 582
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 1 cut(s) 562
BssMI GATC 3 cut(s) 91, 328, 464
Bst2UI CCWGG 2 cut(s) 38, 83
Bst4CI ACNGT 2 cut(s) 179, 370
Bst6I CTCTTC 1 cut(s) 198
BstAPI GCANNNNNTGC 1 cut(s) 392
BstC8I GCNNGC 2 cut(s) 119, 552
BstDEI CTNAG 4 cut(s) 130, 254, 498, 594
BstF5I GGATG 4 cut(s) 46, 154, 437, 470
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 3 cut(s) 94, 331, 467
BstMAI GTCTC 1 cut(s) 597
BstMBI GATC 3 cut(s) 91, 328, 464
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 2 cut(s) 392, 539
BstNI CCWGG 2 cut(s) 38, 83
BstNSI RCATGY 1 cut(s) 58
BstSCI CCNGG 2 cut(s) 36, 81
BstSFI CTRYAG 1 cut(s) 266
BstV1I GCAGC 3 cut(s) 252, 280, 379
BstV2I GAAGAC 2 cut(s) 57, 203
BstX2I RGATCY 3 cut(s) 91, 328, 464
BstXI CCANNNNNNTGG 1 cut(s) 481
BstYI RGATCY 3 cut(s) 91, 328, 464
BtsCI GGATG 4 cut(s) 46, 154, 437, 470
BtsI GCAGTG 1 cut(s) 361
BtsIMutI CAGTG 2 cut(s) 361, 602
Cac8I GCNNGC 2 cut(s) 119, 552
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 106
CseI GACGC 1 cut(s) 579
Csp6I GTAC 1 cut(s) 585
CviAII CATG 3 cut(s) 55, 139, 308
CviQI GTAC 1 cut(s) 585
DdeI CTNAG 4 cut(s) 130, 254, 498, 594
DpnI GATC 3 cut(s) 93, 330, 466
DpnII GATC 3 cut(s) 91, 328, 464
DriI GACNNNNNGTC 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 198
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 36, 81
Esp3I CGTCTC 1 cut(s) 597
FaeI CATG 3 cut(s) 58, 142, 311
FaqI GGGAC 1 cut(s) 417
FatI CATG 3 cut(s) 54, 138, 307
FauNDI CATATG 1 cut(s) 165
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 266, 269, 393
FokI GGATG 4 cut(s) 53, 161, 424, 457
Fsp4HI GCNGC 3 cut(s) 266, 269, 393
FspBI CTAG 1 cut(s) 351
FspI TGCGCA 1 cut(s) 160
GlaI GCGC 1 cut(s) 160
GluI GCNGC 3 cut(s) 266, 269, 393
GsuI CTGGAG 1 cut(s) 329
HapII CCGG 3 cut(s) 118, 419, 449
HgaI GACGC 1 cut(s) 579
HhaI GCGC 1 cut(s) 161
Hin1II CATG 3 cut(s) 58, 142, 311
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 2 cut(s) 311, 493
HpaII CCGG 3 cut(s) 118, 419, 449
HphI GGTGA 1 cut(s) 571
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 4 cut(s) 255, 333, 566, 611
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 1 cut(s) 70
HpyCH4III ACNGT 2 cut(s) 179, 370
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 5 cut(s) 101, 268, 446, 521, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 539
HpyF3I CTNAG 4 cut(s) 130, 254, 498, 594
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 3 cut(s) 58, 142, 311
HspAI GCGC 1 cut(s) 159
KroI GCCGGC 1 cut(s) 117
KroNI GCCGGC 1 cut(s) 119
Kzo9I GATC 3 cut(s) 91, 328, 464
LmnI GCTCC 4 cut(s) 320, 348, 421, 547
Lsp1109I GCAGC 3 cut(s) 252, 280, 379
LweI GCATC 3 cut(s) 31, 88, 287
MaeI CTAG 1 cut(s) 351
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 3 cut(s) 170, 370, 577
MalI GATC 3 cut(s) 93, 330, 466
MboI GATC 3 cut(s) 91, 328, 464
MboII GAAGA 7 cut(s) 62, 154, 164, 208, 215, 390, 538
MflI RGATCY 3 cut(s) 91, 328, 464
MluCI AATT 2 cut(s) 288, 534
MlyI GAGTC 1 cut(s) 320
MmeI TCCRAC 2 cut(s) 298, 498
MnlI CCTC 5 cut(s) 205, 235, 238, 445, 572
MroNI GCCGGC 1 cut(s) 117
MseI TTAA 3 cut(s) 291, 377, 537
MslI CAYNNNNRTG 1 cut(s) 143
MspI CCGG 3 cut(s) 118, 419, 449
MspR9I CCNGG 2 cut(s) 38, 83
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 2 cut(s) 38, 83
MwoI GCNNNNNNNGC 2 cut(s) 392, 539
NaeI GCCGGC 1 cut(s) 119
NdeI CATATG 1 cut(s) 165
NdeII GATC 3 cut(s) 91, 328, 464
NgoMIV GCCGGC 1 cut(s) 117
NlaIII CATG 3 cut(s) 58, 142, 311
NlaIV GGNNCC 3 cut(s) 93, 423, 486
NmuCI GTSAC 1 cut(s) 577
NsbI TGCGCA 1 cut(s) 160
NspI RCATGY 1 cut(s) 58
PctI GAATGC 1 cut(s) 535
PdiI GCCGGC 1 cut(s) 119
PfeI GAWTC 1 cut(s) 493
PflMI CCANNNNNTGG 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 81
PkrI GCNGC 3 cut(s) 267, 270, 394
PleI GAGTC 1 cut(s) 319
PpsI GAGTC 1 cut(s) 319
PshBI ATTAAT 1 cut(s) 291
Psp6I CCWGG 2 cut(s) 36, 81
PspGI CCWGG 2 cut(s) 36, 81
PspN4I GGNNCC 3 cut(s) 93, 423, 486
PspPI GGNCC 1 cut(s) 106
PstI CTGCAG 1 cut(s) 270
PsuI RGATCY 3 cut(s) 91, 328, 464
RsaI GTAC 1 cut(s) 586
RsaNI GTAC 1 cut(s) 585
RseI CAYNNNNRTG 1 cut(s) 143
SaqAI TTAA 3 cut(s) 291, 377, 537
SatI GCNGC 3 cut(s) 266, 269, 393
Sau3AI GATC 3 cut(s) 91, 328, 464
Sau96I GGNCC 1 cut(s) 106
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 2 cut(s) 38, 83
SetI ASST 6 cut(s) 234, 253, 260, 267, 345, 552
SfaNI GCATC 3 cut(s) 31, 88, 287
SfcI CTRYAG 1 cut(s) 266
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 143
Sse9I AATT 2 cut(s) 288, 534
SsiI CCGC 2 cut(s) 58, 542
SspMI CTAG 1 cut(s) 351
StyD4I CCNGG 2 cut(s) 36, 81
TaaI ACNGT 2 cut(s) 179, 370
TaiI ACGT 1 cut(s) 234
TaqII GACCGA 1 cut(s) 123
TasI AATT 2 cut(s) 288, 534
TatI WGTACW 1 cut(s) 584
TfiI GAWTC 1 cut(s) 493
Tru1I TTAA 3 cut(s) 291, 377, 537
Tru9I TTAA 3 cut(s) 291, 377, 537
TscAI CASTG 2 cut(s) 361, 602
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 3 cut(s) 265, 268, 392
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 2 cut(s) 155, 165
TspGWI ACGGA 2 cut(s) 215, 304
TspRI CASTG 2 cut(s) 361, 602
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 106
VspI ATTAAT 1 cut(s) 291
XceI RCATGY 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 1 cut(s) 353
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 1 cut(s) 351
ZrmI AGTACT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.