Rmu_sc0004849.1_g000001

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004849.1
OG0000286
Physical Location & Seq
Reverse (-)
2845 .. 3918
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004849.1_g000001.1.cds

Sequence Viewer

Length: 819 bp
atggaggagttgcgacagacggcattagcatattaccaggatgccccagaagacatgcggtcggcagtagaacattttttccaggagatggatcgtgatgcaaaggaccgagtgagccggcaagatttcttagaatacatgaagatggatgaagattgcgcacatatgagtaaccacgggttctttgatgtgctgaagaaggaagagagcgaggagctggatatcatggacgtgatgactctcctctacatactatatagtgggagaccattctgtaatggagactgcaaaaacttcattaagggaagctatttcacttgcgtcaaatgctatgataattccaccgatacattcaacgtttgcgcggcctgttatcttgacgccaactatgtccaccaacacgaaaagttccttgataactatcttctgtcagaagccaagaggagggaagctctgagcttgaagctgcggcaggaagcatcaaacttaattaatgagtcaaaggcacatgagtccgttggagcaaaagatctgaaaaagaagctccagtctagcagtggcagtagtacagtaacttcaagagaagcaaacgcttcttcttcttctccaaaaccaccgacgacaccggagcccagccgttccacatccaatgcactggagggaagtaatagctccacatccaacgctctggttcccgtgaatcctaagcctaaacatagaagtagtgcagggaagaaagcaattaagacggcggagttggcacttgccctgggaaatatttttctaaccagtactgcgtctcagtgtgccattctctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5

Protein Analysis

272

Amino Acids

30.34

Weight (kDa)

5.85

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 160
AccB7I CCANNNNNTGG 1 cut(s) 88
AccII CGCG 1 cut(s) 365
AciI CCGC 4 cut(s) 58, 365, 469, 752
AclI AACGTT 1 cut(s) 357
AclWI GGATC 1 cut(s) 99
AcuI CTGAAG 1 cut(s) 215
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 2 cut(s) 568, 793
AfiI CCNNNNNNNGG 2 cut(s) 88, 444
AgsI TTSAA 3 cut(s) 355, 463, 579
AhdI GACNNNNNGTC 1 cut(s) 58
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 3 cut(s) 36, 81, 768
AloI GAACNNNNNNTCC 2 cut(s) 63, 95
AluBI AGCT 7 cut(s) 217, 309, 452, 459, 466, 544, 672
AluI AGCT 7 cut(s) 217, 309, 452, 459, 466, 544, 672
Alw26I GTCTC 3 cut(s) 259, 276, 804
AlwI GGATC 1 cut(s) 99
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 1 cut(s) 466
AseI ATTAAT 1 cut(s) 492
AspLEI GCGC 2 cut(s) 161, 365
AspS9I GGNCC 1 cut(s) 106
AvaII GGWCC 1 cut(s) 106
BanII GRGCYC 1 cut(s) 633
BarI GAAGNNNNNNTAC 2 cut(s) 559, 591
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 453
BccI CCATC 2 cut(s) 82, 139
BceAI ACGGC 3 cut(s) 36, 621, 765
BciT130I CCWGG 3 cut(s) 38, 83, 770
BcoDI GTCTC 3 cut(s) 259, 276, 804
BfaI CTAG 1 cut(s) 552
BglII AGATCT 1 cut(s) 529
BisI GCNGC 3 cut(s) 366, 467, 470
BlsI GCNGC 3 cut(s) 367, 468, 471
BmcAI AGTACT 1 cut(s) 793
Bme1390I CCNGG 3 cut(s) 38, 83, 770
Bme18I GGWCC 1 cut(s) 106
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 2 cut(s) 630, 693
BmrFI CCNGG 3 cut(s) 38, 83, 770
BmsI GCATC 3 cut(s) 31, 88, 488
BpiI GAAGAC 1 cut(s) 57
BplI GAGNNNNNCTC 2 cut(s) 436, 468
BpmI CTGGAG 2 cut(s) 530, 677
Bpu10I CCTNAGC 1 cut(s) 705
BsaBI GATNNNNATC 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 3 cut(s) 175, 768, 769
BsaWI WCCGGW 1 cut(s) 625
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 444
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 3 cut(s) 547, 660, 789
Bse8I GATNNNNATC 1 cut(s) 420
BseBI CCWGG 3 cut(s) 38, 83, 770
BseDI CCNNGG 3 cut(s) 175, 768, 769
BseGI GGATG 4 cut(s) 46, 154, 644, 677
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 2 cut(s) 88, 444
BseMII CTCAG 2 cut(s) 446, 815
BseNI ACTGG 3 cut(s) 547, 660, 789
BseRI GAGGAG 4 cut(s) 20, 227, 233, 457
BseXI GCAGC 1 cut(s) 453
BseYI CCCAGC 1 cut(s) 632
BsgI GTGCAG 1 cut(s) 747
Bsh1236I CGCG 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 1 cut(s) 368
BsiEI CGRYCG 1 cut(s) 62
BsiSI CCGG 2 cut(s) 118, 626
BslI CCNNNNNNNGG 2 cut(s) 88, 444
BsmAI GTCTC 3 cut(s) 259, 276, 804
BsmBI CGTCTC 1 cut(s) 804
BsnI GGCC 1 cut(s) 368
Bso31I GGTCTC 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 633
Bsp143I GATC 2 cut(s) 91, 529
BspACI CCGC 4 cut(s) 58, 365, 469, 752
BspANI GGCC 1 cut(s) 368
BspCNI CTCAG 2 cut(s) 447, 814
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 2 cut(s) 630, 693
BspPI GGATC 1 cut(s) 99
BspTNI GGTCTC 1 cut(s) 259
BsrFI RCCGGY 1 cut(s) 117
BsrI ACTGG 3 cut(s) 547, 660, 789
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 3 cut(s) 175, 768, 769
BssMI GATC 2 cut(s) 91, 529
BssNI GRCGYC 1 cut(s) 381
Bst2UI CCWGG 3 cut(s) 38, 83, 770
Bst4CI ACNGT 1 cut(s) 571
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 4 cut(s) 130, 455, 705, 801
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 4 cut(s) 46, 154, 644, 677
BstFNI CGCG 1 cut(s) 365
BstHHI GCGC 2 cut(s) 161, 365
BstKTI GATC 2 cut(s) 94, 532
BstMAI GTCTC 3 cut(s) 259, 276, 804
BstMBI GATC 2 cut(s) 91, 529
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 2 cut(s) 327, 758
BstNI CCWGG 3 cut(s) 38, 83, 770
BstNSI RCATGY 1 cut(s) 58
BstSCI CCNGG 3 cut(s) 36, 81, 768
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 1 cut(s) 453
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 529
BstXI CCANNNNNNTGG 2 cut(s) 655, 688
BstYI RGATCY 1 cut(s) 529
BsuRI GGCC 1 cut(s) 368
BtgI CCRYGG 1 cut(s) 175
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 4 cut(s) 46, 154, 644, 677
BtsI GCAGTG 1 cut(s) 562
BtsIMutI CAGTG 3 cut(s) 562, 653, 809
Cac8I GCNNGC 1 cut(s) 119
CfoI GCGC 2 cut(s) 161, 365
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 106
CseI GACGC 3 cut(s) 310, 389, 786
Csp6I GTAC 2 cut(s) 567, 792
CviAII CATG 4 cut(s) 55, 139, 226, 509
CviQI GTAC 2 cut(s) 567, 792
DdeI CTNAG 4 cut(s) 130, 455, 705, 801
DpnI GATC 2 cut(s) 93, 531
DpnII GATC 2 cut(s) 91, 529
DriI GACNNNNNGTC 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 198
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 198
EciI GGCGGA 1 cut(s) 767
Eco24I GRGCYC 1 cut(s) 633
Eco31I GGTCTC 1 cut(s) 259
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 215
EcoRII CCWGG 3 cut(s) 36, 81, 768
EcoRV GATATC 1 cut(s) 223
EcoT38I GRGCYC 1 cut(s) 633
Esp3I CGTCTC 1 cut(s) 804
FaeI CATG 4 cut(s) 58, 142, 229, 512
FatI CATG 4 cut(s) 54, 138, 225, 508
FauNDI CATATG 1 cut(s) 165
Fnu4HI GCNGC 3 cut(s) 366, 467, 470
FokI GGATG 4 cut(s) 53, 161, 631, 664
FriOI GRGCYC 1 cut(s) 633
Fsp4HI GCNGC 3 cut(s) 366, 467, 470
FspBI CTAG 1 cut(s) 552
FspI TGCGCA 1 cut(s) 160
GlaI GCGC 2 cut(s) 160, 364
GluI GCNGC 3 cut(s) 366, 467, 470
GsaI CCCAGC 1 cut(s) 636
GsuI CTGGAG 2 cut(s) 530, 677
HaeIII GGCC 1 cut(s) 368
HapII CCGG 2 cut(s) 118, 626
HgaI GACGC 3 cut(s) 310, 389, 786
HhaI GCGC 2 cut(s) 161, 365
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 4 cut(s) 58, 142, 229, 512
Hin6I GCGC 2 cut(s) 159, 363
HinP1I GCGC 2 cut(s) 159, 363
HinfI GANTC 4 cut(s) 238, 497, 512, 700
HpaII CCGG 2 cut(s) 118, 626
Hpy166II GTNNAC 1 cut(s) 394
Hpy188I TCNGA 4 cut(s) 433, 456, 534, 818
Hpy188III TCNNGA 3 cut(s) 95, 377, 579
Hpy8I GTNNAC 1 cut(s) 394
Hpy99I CGWCG 1 cut(s) 622
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 571
HpyCH4IV ACGT 2 cut(s) 231, 357
HpyCH4V TGCA 4 cut(s) 101, 288, 653, 728
HpyF10VI GCNNNNNNNGC 2 cut(s) 327, 758
HpyF3I CTNAG 4 cut(s) 130, 455, 705, 801
HpySE526I ACGT 2 cut(s) 231, 357
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 4 cut(s) 58, 142, 229, 512
HspAI GCGC 2 cut(s) 159, 363
KroI GCCGGC 1 cut(s) 117
KroNI GCCGGC 1 cut(s) 119
Kzo9I GATC 2 cut(s) 91, 529
LmnI GCTCC 5 cut(s) 214, 521, 549, 628, 677
Lsp1109I GCAGC 1 cut(s) 453
LweI GCATC 3 cut(s) 31, 88, 488
MaeI CTAG 1 cut(s) 552
MaeII ACGT 2 cut(s) 231, 357
MaeIII GTNAC 2 cut(s) 170, 571
MalI GATC 2 cut(s) 93, 531
MboI GATC 2 cut(s) 91, 529
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 1 cut(s) 633
MluCI AATT 3 cut(s) 337, 489, 741
MlyI GAGTC 3 cut(s) 232, 506, 521
MmeI TCCRAC 2 cut(s) 499, 705
MnlI CCTC 5 cut(s) 205, 254, 435, 438, 652
MroNI GCCGGC 1 cut(s) 117
MseI TTAA 4 cut(s) 300, 488, 492, 744
MslI CAYNNNNRTG 2 cut(s) 143, 230
MspI CCGG 2 cut(s) 118, 626
MspR9I CCNGG 3 cut(s) 38, 83, 770
MvaI CCWGG 3 cut(s) 38, 83, 770
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 2 cut(s) 327, 758
NaeI GCCGGC 1 cut(s) 119
NdeI CATATG 1 cut(s) 165
NdeII GATC 2 cut(s) 91, 529
NgoMIV GCCGGC 1 cut(s) 117
NlaIII CATG 4 cut(s) 58, 142, 229, 512
NlaIV GGNNCC 2 cut(s) 630, 693
NsbI TGCGCA 1 cut(s) 160
NspI RCATGY 1 cut(s) 58
PacI TTAATTAA 1 cut(s) 492
PasI CCCWGGG 1 cut(s) 769
PdiI GCCGGC 1 cut(s) 119
PfeI GAWTC 1 cut(s) 700
PflMI CCANNNNNTGG 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 81
PkrI GCNGC 3 cut(s) 367, 468, 471
PleI GAGTC 3 cut(s) 232, 505, 520
PpsI GAGTC 3 cut(s) 232, 505, 520
PshBI ATTAAT 1 cut(s) 492
Psp1406I AACGTT 1 cut(s) 357
Psp6I CCWGG 3 cut(s) 36, 81, 768
PspFI CCCAGC 1 cut(s) 632
PspGI CCWGG 3 cut(s) 36, 81, 768
PspN4I GGNNCC 2 cut(s) 630, 693
PspPI GGNCC 1 cut(s) 106
PsuI RGATCY 1 cut(s) 529
RsaI GTAC 2 cut(s) 568, 793
RsaNI GTAC 2 cut(s) 567, 792
RseI CAYNNNNRTG 2 cut(s) 143, 230
SaqAI TTAA 4 cut(s) 300, 488, 492, 744
SatI GCNGC 3 cut(s) 366, 467, 470
Sau3AI GATC 2 cut(s) 91, 529
Sau96I GGNCC 1 cut(s) 106
ScaI AGTACT 1 cut(s) 793
SchI GAGTC 3 cut(s) 232, 506, 521
ScrFI CCNGG 3 cut(s) 38, 83, 770
SduI GDGCHC 1 cut(s) 633
SetI ASST 9 cut(s) 219, 234, 311, 360, 454, 461, 468, 546, 674
SfaNI GCATC 3 cut(s) 31, 88, 488
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 2 cut(s) 143, 230
Sse9I AATT 3 cut(s) 337, 489, 741
SsiI CCGC 4 cut(s) 58, 365, 469, 752
SspI AATATT 1 cut(s) 778
SspMI CTAG 1 cut(s) 552
StyD4I CCNGG 3 cut(s) 36, 81, 768
TaaI ACNGT 1 cut(s) 571
TaiI ACGT 2 cut(s) 234, 360
TaqII GACCGA 1 cut(s) 123
TasI AATT 3 cut(s) 337, 489, 741
TatI WGTACW 2 cut(s) 566, 791
TauI GCSGC 2 cut(s) 368, 472
TfiI GAWTC 1 cut(s) 700
Tru1I TTAA 4 cut(s) 300, 488, 492, 744
Tru9I TTAA 4 cut(s) 300, 488, 492, 744
TscAI CASTG 3 cut(s) 562, 660, 809
TseI GCWGC 1 cut(s) 466
TspDTI ATGAA 3 cut(s) 155, 165, 286
TspGWI ACGGA 1 cut(s) 505
TspRI CASTG 3 cut(s) 562, 660, 809
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 106
VspI ATTAAT 1 cut(s) 492
XceI RCATGY 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
XspI CTAG 1 cut(s) 552
ZrmI AGTACT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.