Prupe.4G134500_v2.0.a1

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000286
Physical Location & Seq
Reverse (-)
7434086 .. 7434735
650 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G134500.1

Sequence Viewer

Length: 558 bp
ATGGAGGAACTACACCAGGCAGCTTTAGCATATTACAACAATGGGTCACAAAATCTTCAGCTGCTGGCTGCCAACTTCTTCCGATCCATGGACAGAAATGGCGATGGTCGAGTAAGCAAGTCTGAGTTCATTACATTTCTTCGGCAGAACGGCTATGGTTGGATTAACCCTAGCTTCTTCAACAATCTGGATCGAAACCGTGATGGCGGTCTGGATTTTGAAGAAGTGCTTACATTTTACTACATCATTAAGACCAGGAACTTGTGGTGCGATGGTTGTGGGGTGTGTCTAAAAGGGTTGTATTTTACGTGTGTTGAGTGCTTCGACAGAGCCGCCAACACGTATGATCTATGCTCCCGTTGCTACTCCAGCAGGCGGTTCCGCCACAACCACACCTGCTTCTTGGATAACCATATGCTGCTGAGATCAAAGAGAACGCAACTTAGACAAGCTGTTAAAGATTTTCTTTTGGATACAATTCTGTTAATGAAAACAATCATTGATGCAGGTAATCACTCCAGCACGACAAATTTTCTACCCTCCAGCTACACCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8 ZZ

Protein Analysis

186

Amino Acids

21.5

Weight (kDa)

8.37

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 404
Acc36I ACCTGC 2 cut(s) 404, 497
AciI CCGC 4 cut(s) 207, 333, 376, 382
AclWI GGATC 2 cut(s) 78, 198
AcsI RAATTY 1 cut(s) 529
AcuI CTGAAG 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 88, 375
AflIII ACRYGT 2 cut(s) 308, 339
AgsI TTSAA 2 cut(s) 181, 221
AjnI CCWGG 2 cut(s) 15, 254
AluBI AGCT 5 cut(s) 23, 61, 174, 452, 546
AluI AGCT 5 cut(s) 23, 61, 174, 452, 546
AlwI GGATC 2 cut(s) 78, 198
AlwNI CAGNNNCTG 1 cut(s) 64
ApeKI GCWGC 4 cut(s) 20, 61, 68, 418
ApoI RAATTY 1 cut(s) 529
BbvI GCAGC 4 cut(s) 32, 48, 55, 405
BccI CCATC 3 cut(s) 98, 197, 266
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 2 cut(s) 17, 256
BciVI GTATCC 1 cut(s) 466
BfaI CTAG 2 cut(s) 171, 556
BfuAI ACCTGC 2 cut(s) 404, 497
BfuI GTATCC 1 cut(s) 466
BisI GCNGC 5 cut(s) 21, 62, 69, 333, 419
BlsI GCNGC 5 cut(s) 22, 63, 70, 334, 420
Bme1390I CCNGG 2 cut(s) 17, 256
BmiI GGNNCC 1 cut(s) 380
BmrFI CCNGG 2 cut(s) 17, 256
BmsI GCATC 1 cut(s) 493
BpmI CTGGAG 3 cut(s) 352, 502, 526
BsaAI YACGTR 2 cut(s) 309, 342
BsaJI CCNNGG 1 cut(s) 87
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 375
BseBI CCWGG 2 cut(s) 17, 256
BseDI CCNNGG 1 cut(s) 87
BseLI CCNNNNNNNGG 2 cut(s) 88, 375
BseMII CTCAG 2 cut(s) 114, 413
BseXI GCAGC 4 cut(s) 32, 48, 55, 405
BslI CCNNNNNNNGG 2 cut(s) 88, 375
Bsp143I GATC 4 cut(s) 83, 190, 346, 425
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 4 cut(s) 207, 333, 376, 382
BspCNI CTCAG 2 cut(s) 115, 414
BspLI GGNNCC 1 cut(s) 380
BspMI ACCTGC 2 cut(s) 404, 497
BspPI GGATC 2 cut(s) 78, 198
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 4 cut(s) 83, 190, 346, 425
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 2 cut(s) 17, 256
Bst4CI ACNGT 1 cut(s) 200
BstBAI YACGTR 2 cut(s) 309, 342
BstC8I GCNNGC 2 cut(s) 66, 374
BstDEI CTNAG 3 cut(s) 123, 422, 443
BstDSI CCRYGG 1 cut(s) 87
BstKTI GATC 4 cut(s) 86, 193, 349, 428
BstMBI GATC 4 cut(s) 83, 190, 346, 425
BstMWI GCNNNNNNNGC 4 cut(s) 26, 360, 369, 552
BstNI CCWGG 2 cut(s) 17, 256
BstSCI CCNGG 2 cut(s) 15, 254
BstV1I GCAGC 4 cut(s) 32, 48, 55, 405
BsuI GTATCC 1 cut(s) 466
BtgI CCRYGG 1 cut(s) 87
BtgZI GCGATG 2 cut(s) 117, 285
BveI ACCTGC 2 cut(s) 404, 497
Cac8I GCNNGC 2 cut(s) 66, 374
CaiI CAGNNNCTG 1 cut(s) 64
CviAII CATG 1 cut(s) 88
CviJI RGCY 8 cut(s) 23, 61, 68, 153, 174, 332, 452, 546
CviKI_1 RGCY 8 cut(s) 23, 61, 68, 153, 174, 332, 452, 546
DdeI CTNAG 3 cut(s) 123, 422, 443
DpnI GATC 4 cut(s) 85, 192, 348, 427
DpnII GATC 4 cut(s) 83, 190, 346, 425
EciI GGCGGA 1 cut(s) 371
Eco130I CCWWGG 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 41
EcoRII CCWGG 2 cut(s) 15, 254
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 1 cut(s) 91
FaiI YATR 7 cut(s) 31, 89, 156, 345, 352, 414, 416
FalI AAGNNNNNCTT 4 cut(s) 213, 245, 450, 482
FatI CATG 1 cut(s) 87
FauNDI CATATG 1 cut(s) 414
Fnu4HI GCNGC 5 cut(s) 21, 62, 69, 333, 419
Fsp4HI GCNGC 5 cut(s) 21, 62, 69, 333, 419
FspBI CTAG 2 cut(s) 171, 556
GluI GCNGC 5 cut(s) 21, 62, 69, 333, 419
GsuI CTGGAG 3 cut(s) 352, 502, 526
Hin1II CATG 1 cut(s) 91
Hpy188I TCNGA 2 cut(s) 83, 124
Hpy188III TCNNGA 2 cut(s) 188, 212
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4IV ACGT 2 cut(s) 308, 341
HpyCH4V TGCA 1 cut(s) 506
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 360, 369, 552
HpyF3I CTNAG 3 cut(s) 123, 422, 443
HpySE526I ACGT 2 cut(s) 308, 341
Hsp92II CATG 1 cut(s) 91
Kzo9I GATC 4 cut(s) 83, 190, 346, 425
LmnI GCTCC 1 cut(s) 359
Lsp1109I GCAGC 4 cut(s) 32, 48, 55, 405
LweI GCATC 1 cut(s) 493
MaeI CTAG 2 cut(s) 171, 556
MaeII ACGT 2 cut(s) 308, 341
MaeIII GTNAC 1 cut(s) 45
MalI GATC 4 cut(s) 85, 192, 348, 427
MboI GATC 4 cut(s) 83, 190, 346, 425
MboII GAAGA 5 cut(s) 47, 70, 131, 169, 233
MluCI AATT 2 cut(s) 477, 529
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 1 cut(s) 550
MseI TTAA 4 cut(s) 165, 249, 456, 485
MspA1I CMGCKG 1 cut(s) 61
MspR9I CCNGG 2 cut(s) 17, 256
MvaI CCWGG 2 cut(s) 17, 256
MwoI GCNNNNNNNGC 4 cut(s) 26, 360, 369, 552
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 414
NdeII GATC 4 cut(s) 83, 190, 346, 425
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 1 cut(s) 380
NmuCI GTSAC 1 cut(s) 45
PaqCI CACCTGC 1 cut(s) 404
PkrI GCNGC 5 cut(s) 22, 63, 70, 334, 420
Ppu21I YACGTR 2 cut(s) 309, 342
Psp6I CCWGG 2 cut(s) 15, 254
PspGI CCWGG 2 cut(s) 15, 254
PspN4I GGNNCC 1 cut(s) 380
PstNI CAGNNNCTG 1 cut(s) 64
PvuII CAGCTG 1 cut(s) 61
SaqAI TTAA 4 cut(s) 165, 249, 456, 485
SatI GCNGC 5 cut(s) 21, 62, 69, 333, 419
Sau3AI GATC 4 cut(s) 83, 190, 346, 425
ScrFI CCNGG 2 cut(s) 17, 256
SfaNI GCATC 1 cut(s) 493
Sse9I AATT 2 cut(s) 477, 529
SsiI CCGC 4 cut(s) 207, 333, 376, 382
SspMI CTAG 2 cut(s) 171, 556
StyD4I CCNGG 2 cut(s) 15, 254
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 200
TaiI ACGT 2 cut(s) 311, 344
TaqI TCGA 3 cut(s) 109, 193, 324
TasI AATT 2 cut(s) 477, 529
TauI GCSGC 1 cut(s) 335
Tru1I TTAA 4 cut(s) 165, 249, 456, 485
Tru9I TTAA 4 cut(s) 165, 249, 456, 485
TseFI GTSAC 1 cut(s) 45
TseI GCWGC 4 cut(s) 20, 61, 68, 418
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 2 cut(s) 118, 503
XapI RAATTY 1 cut(s) 529
XspI CTAG 2 cut(s) 171, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.