Rh4AG060000

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000286
Physical Location & Seq
Reverse (-)
12496679 .. 12499711
3033 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG060000.1

Sequence Viewer

Length: 642 bp
ATGGGAGACATCAAAGAAATCCGTGATGCTTCTTTAGCTTATTACCATAACTTGTCTGAAAAGCAAAAGCATGAGGTGTCTACCTTTTTTCAAGGAATAGATACAGATGGTGATGGAACAATAACCATGGAGGAGTTCAAGCAGCTGTTTGAGGGTTGTTTCGGCGACCGTGTCAACCGTATCTTTCAGAAGCTAGACAAAAATGGAGATGGTAGTTTGGATTTTGAGGAATCTGTCACTCTTTACTACTCTCTTATAACTGATAACATTATTAGTTGCGATGGATGCAATGCCCCTTTTCTAAAGGGTCTGTATTTCACTTGCGTGGAGTGCTTCGAGCATAACCAAACTACAACTTTTGATCTTTGCACCTCCTGCTACCGCAGCCATAACTACATTCACGAACACTGCATCTTTTTGGACAACAACGCCTTACTGCGTACCAAGGCTGCTTATAACAATTCTGGATCGACAAACCAGCCAACATTTGCCGAGCTTGATGGATCTGATCTTGAGAACAATGAAGTTTCTCGATCGAGATCTAAAAAATTAGCAGCTCTGAAAGTCATCACTACCGGTGTTGAAATCGGAAATGCTGTGGGAAATATTGCTGTTGCCACTGGTACATGCATCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

213

Amino Acids

23.74

Weight (kDa)

4.85

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 22 - 52 3.5e-06 EF-hand domain pair
EF-hand_1 PF00036 26 - 51 7.1e-06 EF hand domain
EF-hand_5 PF13202 29 - 49 2.8e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 257, 456
AccI GTMKAC 1 cut(s) 80
AciI CCGC 1 cut(s) 382
AclWI GGATC 2 cut(s) 475, 511
AfaI GTAC 2 cut(s) 442, 625
AgeI ACCGGT 1 cut(s) 575
AgsI TTSAA 3 cut(s) 92, 139, 584
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 5 cut(s) 38, 145, 193, 496, 557
AluI AGCT 5 cut(s) 38, 145, 193, 496, 557
AlwI GGATC 2 cut(s) 475, 511
ApeKI GCWGC 4 cut(s) 142, 384, 449, 554
AsiGI ACCGGT 1 cut(s) 575
AsuHPI GGTGA 1 cut(s) 122
BbvI GCAGC 4 cut(s) 154, 396, 436, 566
BccI CCATC 5 cut(s) 101, 107, 203, 275, 494
BfaI CTAG 1 cut(s) 194
BglII AGATCT 1 cut(s) 539
BisI GCNGC 4 cut(s) 143, 385, 450, 555
BlsI GCNGC 4 cut(s) 144, 386, 451, 556
BmsI GCATC 3 cut(s) 16, 275, 420
BpuEI CTTGAG 1 cut(s) 533
BsaBI GATNNNNATC 1 cut(s) 538
BsaJI CCNNGG 2 cut(s) 126, 444
BsaWI WCCGGW 1 cut(s) 575
Bse118I RCCGGY 1 cut(s) 575
Bse1I ACTGG 1 cut(s) 625
Bse3DI GCAATG 1 cut(s) 295
Bse8I GATNNNNATC 1 cut(s) 538
BseDI CCNNGG 2 cut(s) 126, 444
BseGI GGATG 1 cut(s) 290
BseJI GATNNNNATC 1 cut(s) 538
BseMI GCAATG 1 cut(s) 295
BseNI ACTGG 1 cut(s) 625
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 4 cut(s) 154, 396, 436, 566
Bsh1285I CGRYCG 2 cut(s) 169, 536
BshTI ACCGGT 1 cut(s) 575
BsiEI CGRYCG 2 cut(s) 169, 536
BsiSI CCGG 1 cut(s) 576
Bsp143I GATC 6 cut(s) 361, 467, 503, 508, 533, 539
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 1 cut(s) 382
BspPI GGATC 2 cut(s) 475, 511
BsrDI GCAATG 1 cut(s) 295
BsrFI RCCGGY 1 cut(s) 575
BsrI ACTGG 1 cut(s) 625
BssAI RCCGGY 1 cut(s) 575
BssECI CCNNGG 2 cut(s) 126, 444
BssMI GATC 6 cut(s) 361, 467, 503, 508, 533, 539
BssT1I CCWWGG 2 cut(s) 126, 444
Bst4CI ACNGT 2 cut(s) 170, 179
BstAPI GCANNNNNTGC 1 cut(s) 375
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 290
BstKTI GATC 6 cut(s) 364, 470, 506, 511, 536, 542
BstMBI GATC 6 cut(s) 361, 467, 503, 508, 533, 539
BstMCI CGRYCG 2 cut(s) 169, 536
BstMWI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 375, 384
BstNSI RCATGY 1 cut(s) 630
BstV1I GCAGC 4 cut(s) 154, 396, 436, 566
BstX2I RGATCY 2 cut(s) 503, 539
BstYI RGATCY 2 cut(s) 503, 539
BtgI CCRYGG 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 290
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 2 cut(s) 406, 618
Cfr10I RCCGGY 1 cut(s) 575
Csp6I GTAC 2 cut(s) 441, 624
CspAI ACCGGT 1 cut(s) 575
CviAII CATG 3 cut(s) 71, 127, 627
CviJI RGCY 8 cut(s) 38, 145, 193, 387, 449, 481, 496, 557
CviKI_1 RGCY 8 cut(s) 38, 145, 193, 387, 449, 481, 496, 557
CviQI GTAC 2 cut(s) 441, 624
DpnI GATC 6 cut(s) 363, 469, 505, 510, 535, 541
DpnII GATC 6 cut(s) 361, 467, 503, 508, 533, 539
Eco130I CCWWGG 2 cut(s) 126, 444
EcoT14I CCWWGG 2 cut(s) 126, 444
EcoT22I ATGCAT 1 cut(s) 632
ErhI CCWWGG 2 cut(s) 126, 444
FaeI CATG 3 cut(s) 74, 130, 630
FaiI YATR 9 cut(s) 48, 72, 128, 257, 342, 390, 456, 628, 638
FatI CATG 3 cut(s) 70, 126, 626
FblI GTMKAC 1 cut(s) 80
Fnu4HI GCNGC 4 cut(s) 143, 385, 450, 555
FokI GGATG 1 cut(s) 297
Fsp4HI GCNGC 4 cut(s) 143, 385, 450, 555
FspBI CTAG 1 cut(s) 194
GluI GCNGC 4 cut(s) 143, 385, 450, 555
HapII CCGG 1 cut(s) 576
Hin1II CATG 3 cut(s) 74, 130, 630
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 1 cut(s) 230
HpaII CCGG 1 cut(s) 576
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 2 cut(s) 81, 175
Hpy188I TCNGA 5 cut(s) 58, 189, 508, 561, 590
Hpy188III TCNNGA 5 cut(s) 401, 465, 512, 531, 537
Hpy8I GTNNAC 2 cut(s) 81, 175
HpyCH4III ACNGT 2 cut(s) 170, 179
HpyCH4V TGCA 4 cut(s) 288, 369, 411, 630
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 375, 384
Hsp92II CATG 3 cut(s) 74, 130, 630
Kzo9I GATC 6 cut(s) 361, 467, 503, 508, 533, 539
LpnPI CCDG 5 cut(s) 388, 450, 491, 589, 606
Lsp1109I GCAGC 4 cut(s) 154, 396, 436, 566
LweI GCATC 3 cut(s) 16, 275, 420
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 1 cut(s) 235
MalI GATC 6 cut(s) 363, 469, 505, 510, 535, 541
MboI GATC 6 cut(s) 361, 467, 503, 508, 533, 539
MflI RGATCY 2 cut(s) 503, 539
MluCI AATT 2 cut(s) 460, 548
MnlI CCTC 5 cut(s) 67, 124, 145, 220, 382
Mph1103I ATGCAT 1 cut(s) 632
MslI CAYNNNNRTG 2 cut(s) 323, 635
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 1 cut(s) 576
MwoI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 375, 384
NcoI CCATGG 1 cut(s) 126
NdeII GATC 6 cut(s) 361, 467, 503, 508, 533, 539
NlaIII CATG 3 cut(s) 74, 130, 630
NmeAIII GCCGAG 1 cut(s) 517
NmuCI GTSAC 1 cut(s) 235
NsiI ATGCAT 1 cut(s) 632
NspI RCATGY 1 cut(s) 630
OliI CACNNNNGTG 1 cut(s) 323
PfeI GAWTC 1 cut(s) 230
PflFI GACNNNGTC 1 cut(s) 170
PinAI ACCGGT 1 cut(s) 575
PkrI GCNGC 4 cut(s) 144, 386, 451, 556
Ple19I CGATCG 1 cut(s) 536
PsiI TTATAA 2 cut(s) 257, 456
PsuI RGATCY 2 cut(s) 503, 539
PsyI GACNNNGTC 1 cut(s) 170
PvuI CGATCG 1 cut(s) 536
PvuII CAGCTG 1 cut(s) 145
RsaI GTAC 2 cut(s) 442, 625
RsaNI GTAC 2 cut(s) 441, 624
RseI CAYNNNNRTG 2 cut(s) 323, 635
SatI GCNGC 4 cut(s) 143, 385, 450, 555
Sau3AI GATC 6 cut(s) 361, 467, 503, 508, 533, 539
SetI ASST 8 cut(s) 40, 78, 86, 147, 195, 374, 498, 559
SfaNI GCATC 3 cut(s) 16, 275, 420
SmiMI CAYNNNNRTG 2 cut(s) 323, 635
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 2 cut(s) 460, 548
SsiI CCGC 1 cut(s) 382
SspI AATATT 1 cut(s) 607
SspMI CTAG 1 cut(s) 194
StyI CCWWGG 2 cut(s) 126, 444
TaaI ACNGT 2 cut(s) 170, 179
TaqI TCGA 4 cut(s) 336, 470, 532, 536
TasI AATT 2 cut(s) 460, 548
TfiI GAWTC 1 cut(s) 230
TscAI CASTG 2 cut(s) 413, 625
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 4 cut(s) 142, 384, 449, 554
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 1 cut(s) 537
TspGWI ACGGA 1 cut(s) 11
TspRI CASTG 2 cut(s) 413, 625
Tth111I GACNNNGTC 1 cut(s) 170
XceI RCATGY 1 cut(s) 630
XmiI GTMKAC 1 cut(s) 80
XspI CTAG 1 cut(s) 194
Zsp2I ATGCAT 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.