FvH4_3g15000

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000286
Physical Location & Seq
Reverse (-)
9298970 .. 9300381
1412 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g15000.t2

Sequence Viewer

Length: 591 bp
ATGGACGAGCTGCGTCAGGCAGCATCTGCATATTACAACAACGGATCACCCGAGATTGAGCAACTAGCTTGGTGCTTCTTCCAATCCATGGACACAAACTGCGACGGCCAAGTCGACTGGAACGAGTTCTCCGCATTTCTCTACCAAAGCGGCAACCAATGGATCAGCCCCAGCTTCTTCAACGATCTCGACCGCAACCACGACGGCGCGCTGGATTTCATGGAAGTTCTAACTTTTTACTACATCATCAAGACCAGGAGCTTCTGGTGCAGCGTTTGTGGGATTTGTCTCACGGGGCTCTACTTCACTTGTGTTGAGTGCTTTGACAACGTGAACTCAAACACTTACGATCTTTGTCCCGGATGCTACGAGTCCAAGCCGGAAAGGCAAAGGTATAACCGGCAGAGTGATCACCATCACGCTTATTTTATGGATAACCATATGTTGCTCAGATCAAAGAGAGGAGGATATGGTTCTTCAAATCTGGCCATAGATTCACCTGGAGGTGGACCAGGACGGACCAGAATGCGCCAAACGATGAAAGCATTGGATACGGCAGTGAATGTTGCAACTTTGTGTAGCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8 ZZ

Protein Analysis

197

Amino Acids

22.44

Weight (kDa)

5.11

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 20 - 81 4.6e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 88
AccI GTMKAC 1 cut(s) 114
AccII CGCG 1 cut(s) 209
AciI CCGC 3 cut(s) 132, 150, 193
AclWI GGATC 2 cut(s) 52, 170
AcoI YGGCCR 2 cut(s) 106, 486
AfiI CCNNNNNNNGG 2 cut(s) 88, 506
AgsI TTSAA 2 cut(s) 181, 480
AjnI CCWGG 3 cut(s) 254, 499, 511
AloI GAACNNNNNNTCC 2 cut(s) 113, 145
AluBI AGCT 4 cut(s) 10, 68, 174, 261
AluI AGCT 4 cut(s) 10, 68, 174, 261
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 2 cut(s) 52, 170
AlwNI CAGNNNCTG 1 cut(s) 26
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 2 cut(s) 106, 486
ApeKI GCWGC 3 cut(s) 10, 20, 270
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 3 cut(s) 209, 211, 531
AspS9I GGNCC 2 cut(s) 509, 519
AsuC2I CCSGG 1 cut(s) 360
AsuHPI GGTGA 3 cut(s) 39, 404, 489
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 2 cut(s) 509, 519
BaeI ACNNNNGTAYC 2 cut(s) 543, 576
BalI TGGCCA 1 cut(s) 488
BanII GRGCYC 1 cut(s) 300
BbvI GCAGC 2 cut(s) 32, 282
BccI CCATC 1 cut(s) 423
BceAI ACGGC 3 cut(s) 121, 220, 570
BciT130I CCWGG 3 cut(s) 256, 501, 513
BciVI GTATCC 1 cut(s) 544
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 1 cut(s) 360
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 1 cut(s) 65
BfuI GTATCC 1 cut(s) 544
BglI GCCNNNNNGGC 1 cut(s) 385
BisI GCNGC 4 cut(s) 11, 21, 151, 271
BlsI GCNGC 4 cut(s) 12, 22, 152, 272
Bme1390I CCNGG 4 cut(s) 256, 360, 501, 513
Bme18I GGWCC 2 cut(s) 509, 519
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 509, 519
BmrFI CCNGG 4 cut(s) 256, 360, 501, 513
BmsI GCATC 2 cut(s) 32, 353
BpmI CTGGAG 1 cut(s) 522
BpuMI CCSGG 1 cut(s) 360
BsaBI GATNNNNATC 1 cut(s) 414
BsaJI CCNNGG 1 cut(s) 87
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 506
Bse118I RCCGGY 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 3 cut(s) 256, 501, 513
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 368
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 88, 506
BseMII CTCAG 1 cut(s) 463
BseNI ACTGG 1 cut(s) 122
BsePI GCGCGC 1 cut(s) 207
BseRI GAGGAG 1 cut(s) 477
BseXI GCAGC 2 cut(s) 32, 282
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 289
Bsh1236I CGCG 1 cut(s) 209
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 2 cut(s) 108, 488
BsiEI CGRYCG 1 cut(s) 193
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 3 cut(s) 360, 380, 400
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 2 cut(s) 88, 506
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 342
BsmI GAATGC 1 cut(s) 531
BsnI GGCC 2 cut(s) 108, 488
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 6 cut(s) 44, 162, 184, 349, 409, 452
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 3 cut(s) 132, 150, 193
BspANI GGCC 2 cut(s) 108, 488
BspCNI CTCAG 1 cut(s) 462
BspFNI CGCG 1 cut(s) 209
BspPI GGATC 2 cut(s) 52, 170
BsrFI RCCGGY 1 cut(s) 399
BsrI ACTGG 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 87
BssHII GCGCGC 1 cut(s) 207
BssMI GATC 6 cut(s) 44, 162, 184, 349, 409, 452
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 3 cut(s) 256, 501, 513
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 209
BstDEI CTNAG 1 cut(s) 449
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 368
BstFNI CGCG 1 cut(s) 209
BstHHI GCGC 3 cut(s) 209, 211, 531
BstKTI GATC 6 cut(s) 47, 165, 187, 352, 412, 455
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 6 cut(s) 44, 162, 184, 349, 409, 452
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 3 cut(s) 26, 267, 385
BstNI CCWGG 3 cut(s) 256, 501, 513
BstSCI CCNGG 4 cut(s) 254, 358, 499, 511
BstUI CGCG 1 cut(s) 209
BstV1I GCAGC 2 cut(s) 32, 282
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 2 cut(s) 108, 488
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 1 cut(s) 368
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 1 cut(s) 564
Cac8I GCNNGC 1 cut(s) 209
CaiI CAGNNNCTG 1 cut(s) 26
CfoI GCGC 3 cut(s) 209, 211, 531
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 2 cut(s) 509, 519
CseI GACGC 1 cut(s) 2
CviAII CATG 2 cut(s) 88, 220
CviJI RGCY 9 cut(s) 10, 68, 108, 168, 174, 261, 298, 379, 488
CviKI_1 RGCY 9 cut(s) 10, 68, 108, 168, 174, 261, 298, 379, 488
DdeI CTNAG 1 cut(s) 449
DpnI GATC 6 cut(s) 46, 164, 186, 351, 411, 454
DpnII GATC 6 cut(s) 44, 162, 184, 349, 409, 452
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 2 cut(s) 106, 486
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 509, 519
Eco88I CYCGRG 1 cut(s) 50
EcoRII CCWGG 3 cut(s) 254, 499, 511
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 2 cut(s) 91, 223
FaqI GGGAC 1 cut(s) 342
FatI CATG 2 cut(s) 87, 219
FauNDI CATATG 1 cut(s) 441
FbaI TGATCA 1 cut(s) 409
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 4 cut(s) 11, 21, 151, 271
FokI GGATG 1 cut(s) 375
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 4 cut(s) 11, 21, 151, 271
FspBI CTAG 1 cut(s) 65
GlaI GCGC 3 cut(s) 208, 210, 530
GluI GCNGC 4 cut(s) 11, 21, 151, 271
GsaI CCCAGC 1 cut(s) 174
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 2 cut(s) 108, 488
HapII CCGG 3 cut(s) 360, 380, 400
HgaI GACGC 1 cut(s) 2
HhaI GCGC 3 cut(s) 209, 211, 531
Hin1II CATG 2 cut(s) 91, 223
Hin6I GCGC 3 cut(s) 207, 209, 529
HinP1I GCGC 3 cut(s) 207, 209, 529
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 2 cut(s) 371, 494
HpaII CCGG 3 cut(s) 360, 380, 400
HphI GGTGA 3 cut(s) 39, 404, 489
Hpy166II GTNNAC 3 cut(s) 115, 334, 509
Hpy188I TCNGA 1 cut(s) 452
Hpy188III TCNNGA 2 cut(s) 188, 250
Hpy8I GTNNAC 3 cut(s) 115, 334, 509
Hpy99I CGWCG 2 cut(s) 107, 206
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 3 cut(s) 29, 270, 569
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 267, 385
HpyF3I CTNAG 1 cut(s) 449
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 2 cut(s) 91, 223
HspAI GCGC 3 cut(s) 207, 209, 529
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 6 cut(s) 44, 162, 184, 349, 409, 452
LmnI GCTCC 1 cut(s) 258
Lsp1109I GCAGC 2 cut(s) 32, 282
LweI GCATC 2 cut(s) 32, 353
MaeI CTAG 1 cut(s) 65
MaeII ACGT 1 cut(s) 330
MalI GATC 6 cut(s) 46, 164, 186, 351, 411, 454
MboI GATC 6 cut(s) 44, 162, 184, 349, 409, 452
MboII GAAGA 3 cut(s) 70, 169, 468
MhlI GDGCHC 1 cut(s) 300
MlsI TGGCCA 1 cut(s) 488
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 1 cut(s) 380
MnlI CCTC 3 cut(s) 455, 458, 497
Mox20I TGGCCA 1 cut(s) 488
MroXI GAANNNNTTC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 488
Msp20I TGGCCA 1 cut(s) 488
MspI CCGG 3 cut(s) 360, 380, 400
MspR9I CCNGG 4 cut(s) 256, 360, 501, 513
Mva1269I GAATGC 1 cut(s) 531
MvaI CCWGG 3 cut(s) 256, 501, 513
MvnI CGCG 1 cut(s) 209
MwoI GCNNNNNNNGC 3 cut(s) 26, 267, 385
NciI CCSGG 1 cut(s) 360
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 441
NdeII GATC 6 cut(s) 44, 162, 184, 349, 409, 452
NlaIII CATG 2 cut(s) 91, 223
PauI GCGCGC 1 cut(s) 207
PcsI WCGNNNNNNNCGW 3 cut(s) 48, 111, 120
PctI GAATGC 1 cut(s) 531
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 1 cut(s) 494
PflMI CCANNNNNTGG 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 358
PkrI GCNGC 4 cut(s) 12, 22, 152, 272
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp6I CCWGG 3 cut(s) 254, 499, 511
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 3 cut(s) 254, 499, 511
PspPI GGNCC 2 cut(s) 509, 519
PstNI CAGNNNCTG 1 cut(s) 26
PteI GCGCGC 1 cut(s) 207
SalI GTCGAC 1 cut(s) 113
SatI GCNGC 4 cut(s) 11, 21, 151, 271
Sau3AI GATC 6 cut(s) 44, 162, 184, 349, 409, 452
Sau96I GGNCC 2 cut(s) 509, 519
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 4 cut(s) 256, 360, 501, 513
SduI GDGCHC 1 cut(s) 300
SetI ASST 8 cut(s) 12, 70, 176, 263, 333, 395, 502, 508
SfaNI GCATC 2 cut(s) 32, 353
SinI GGWCC 2 cut(s) 509, 519
SsiI CCGC 3 cut(s) 132, 150, 193
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 4 cut(s) 254, 358, 499, 511
StyI CCWWGG 1 cut(s) 87
TaiI ACGT 1 cut(s) 333
TaqI TCGA 2 cut(s) 114, 189
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 1 cut(s) 494
TscAI CASTG 1 cut(s) 564
TseI GCWGC 3 cut(s) 10, 20, 270
TspDTI ATGAA 2 cut(s) 208, 554
TspGWI ACGGA 2 cut(s) 57, 532
TspRI CASTG 1 cut(s) 564
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 509, 519
XmiI GTMKAC 1 cut(s) 114
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.