Rh4CG067400

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000286
Physical Location & Seq
Forward (+)
12628138 .. 12629638
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG067400.1

Sequence Viewer

Length: 756 bp
ATGGAGGAGTTGCGACAGACGGCATTAGCATATTACCAGGATGCCCCAGAAGACATGCGGTCGGCAGTAGAACATTTTTTCCAGGAGATGGATCGTGATGCAAAGGACCGAGTGAGCCGGCAAGATTTCTTAGAATACATGAAGATGGATGAAGATTGCGCACATATGAGTAACCACGGGTTCTTTGATGTGCTGAAGAAGGAAGAGAGCGAGGAGCTGGATATCATGGACGTGATGACTCTCCTCTACATACTATATAGTGGGAGACCATTCTGTAATGGAGACTGCAAAAACTACATTAAGGGAAGCTATTTCACTTGCGTCAAATGCTATGATAATTCCACCGATACATTCAACGTTTGCGCGGCCTGTTATGTTGACACCAAGTATGTCCACCAACACAAAAAATTCCTTGATAAATTTGTGCTGTCAGAAGCCAAGAGGAGGGAAGCTCTGATCTTGAAGCTGCGGCAGGAAGCATCAAACTTAATTAATGAGTCAAAGGCACATGAGTCCGTTGGAGCAATAGATCTGAAAAAGAAGCTCCAGTCTAGCAGTGGCAGTAGTACAGTAACTACAAGAGAAGCAAACGCTTCTTCTTCTTCTCCAAAACCACCGACGACACCGGAGCCAAGCCGTTCCACATCCAATGCACTGGAGGGAAGTAATAGCTCCACATCCAACGCTCTGGTTCCCGTGAATCCTAAGCCTAAACATGTAATATATTTCCTCCATCTCTCTGCTCGTTTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5

Protein Analysis

251

Amino Acids

28.52

Weight (kDa)

6.08

Isoelectric Point (pI)

53.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 160
AccB7I CCANNNNNTGG 1 cut(s) 88
AccII CGCG 1 cut(s) 365
AciI CCGC 3 cut(s) 58, 365, 469
AclI AACGTT 1 cut(s) 357
AclWI GGATC 1 cut(s) 99
AcsI RAATTY 2 cut(s) 407, 419
AcuI CTGAAG 1 cut(s) 215
AfaI GTAC 1 cut(s) 568
AfiI CCNNNNNNNGG 2 cut(s) 88, 444
AflIII ACRYGT 1 cut(s) 715
AgsI TTSAA 2 cut(s) 355, 463
AhdI GACNNNNNGTC 1 cut(s) 58
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 2 cut(s) 36, 81
AloI GAACNNNNNNTCC 2 cut(s) 63, 95
AluBI AGCT 6 cut(s) 217, 309, 452, 466, 544, 672
AluI AGCT 6 cut(s) 217, 309, 452, 466, 544, 672
Alw26I GTCTC 2 cut(s) 259, 276
AlwI GGATC 1 cut(s) 99
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 1 cut(s) 466
ApoI RAATTY 2 cut(s) 407, 419
AseI ATTAAT 1 cut(s) 492
AspLEI GCGC 2 cut(s) 161, 365
AspS9I GGNCC 1 cut(s) 106
AvaII GGWCC 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 453
BccI CCATC 3 cut(s) 82, 139, 741
BceAI ACGGC 2 cut(s) 36, 621
BciT130I CCWGG 2 cut(s) 38, 83
BcoDI GTCTC 2 cut(s) 259, 276
BfaI CTAG 1 cut(s) 552
BglII AGATCT 1 cut(s) 529
BisI GCNGC 3 cut(s) 366, 467, 470
BlsI GCNGC 3 cut(s) 367, 468, 471
Bme1390I CCNGG 2 cut(s) 38, 83
Bme18I GGWCC 1 cut(s) 106
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 2 cut(s) 630, 693
BmrFI CCNGG 2 cut(s) 38, 83
BmsI GCATC 3 cut(s) 31, 88, 488
BpiI GAAGAC 1 cut(s) 57
BplI GAGNNNNNCTC 2 cut(s) 436, 468
BpmI CTGGAG 2 cut(s) 530, 677
Bpu10I CCTNAGC 1 cut(s) 705
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 175
BsaWI WCCGGW 1 cut(s) 625
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 444
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 2 cut(s) 547, 660
BseBI CCWGG 2 cut(s) 38, 83
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 4 cut(s) 46, 154, 644, 677
BseLI CCNNNNNNNGG 2 cut(s) 88, 444
BseNI ACTGG 2 cut(s) 547, 660
BseRI GAGGAG 4 cut(s) 20, 227, 233, 457
BseXI GCAGC 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 1 cut(s) 368
BsiEI CGRYCG 1 cut(s) 62
BsiSI CCGG 2 cut(s) 118, 626
BslI CCNNNNNNNGG 2 cut(s) 88, 444
BsmAI GTCTC 2 cut(s) 259, 276
BsnI GGCC 1 cut(s) 368
Bso31I GGTCTC 1 cut(s) 259
Bsp143I GATC 3 cut(s) 91, 456, 529
BspACI CCGC 3 cut(s) 58, 365, 469
BspANI GGCC 1 cut(s) 368
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 2 cut(s) 630, 693
BspPI GGATC 1 cut(s) 99
BspTNI GGTCTC 1 cut(s) 259
BsrFI RCCGGY 1 cut(s) 117
BsrI ACTGG 2 cut(s) 547, 660
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 3 cut(s) 91, 456, 529
Bst2UI CCWGG 2 cut(s) 38, 83
Bst4CI ACNGT 1 cut(s) 571
Bst6I CTCTTC 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 2 cut(s) 130, 705
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 4 cut(s) 46, 154, 644, 677
BstFNI CGCG 1 cut(s) 365
BstHHI GCGC 2 cut(s) 161, 365
BstKTI GATC 3 cut(s) 94, 459, 532
BstMAI GTCTC 2 cut(s) 259, 276
BstMBI GATC 3 cut(s) 91, 456, 529
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 1 cut(s) 327
BstNI CCWGG 2 cut(s) 38, 83
BstNSI RCATGY 2 cut(s) 58, 719
BstSCI CCNGG 2 cut(s) 36, 81
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 1 cut(s) 453
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 529
BstXI CCANNNNNNTGG 2 cut(s) 655, 688
BstYI RGATCY 1 cut(s) 529
BsuRI GGCC 1 cut(s) 368
BtgI CCRYGG 1 cut(s) 175
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 4 cut(s) 46, 154, 644, 677
BtsI GCAGTG 1 cut(s) 562
BtsIMutI CAGTG 2 cut(s) 562, 653
Cac8I GCNNGC 1 cut(s) 119
CfoI GCGC 2 cut(s) 161, 365
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 106
CseI GACGC 1 cut(s) 310
Csp6I GTAC 1 cut(s) 567
CviAII CATG 5 cut(s) 55, 139, 226, 509, 716
CviQI GTAC 1 cut(s) 567
DdeI CTNAG 2 cut(s) 130, 705
DpnI GATC 3 cut(s) 93, 458, 531
DpnII GATC 3 cut(s) 91, 456, 529
DriI GACNNNNNGTC 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 198
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 259
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 36, 81
EcoRV GATATC 1 cut(s) 223
FaeI CATG 5 cut(s) 58, 142, 229, 512, 719
FatI CATG 5 cut(s) 54, 138, 225, 508, 715
FauNDI CATATG 1 cut(s) 165
Fnu4HI GCNGC 3 cut(s) 366, 467, 470
FokI GGATG 4 cut(s) 53, 161, 631, 664
Fsp4HI GCNGC 3 cut(s) 366, 467, 470
FspBI CTAG 1 cut(s) 552
FspI TGCGCA 1 cut(s) 160
GlaI GCGC 2 cut(s) 160, 364
GluI GCNGC 3 cut(s) 366, 467, 470
GsuI CTGGAG 2 cut(s) 530, 677
HaeIII GGCC 1 cut(s) 368
HapII CCGG 2 cut(s) 118, 626
HgaI GACGC 1 cut(s) 310
HhaI GCGC 2 cut(s) 161, 365
Hin1II CATG 5 cut(s) 58, 142, 229, 512, 719
Hin6I GCGC 2 cut(s) 159, 363
HinP1I GCGC 2 cut(s) 159, 363
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 4 cut(s) 238, 497, 512, 700
HpaII CCGG 2 cut(s) 118, 626
Hpy166II GTNNAC 2 cut(s) 379, 394
Hpy188I TCNGA 3 cut(s) 433, 456, 534
Hpy188III TCNNGA 2 cut(s) 95, 460
Hpy8I GTNNAC 2 cut(s) 379, 394
Hpy99I CGWCG 1 cut(s) 622
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 571
HpyCH4IV ACGT 2 cut(s) 231, 357
HpyCH4V TGCA 3 cut(s) 101, 288, 653
HpyF10VI GCNNNNNNNGC 1 cut(s) 327
HpyF3I CTNAG 2 cut(s) 130, 705
HpySE526I ACGT 2 cut(s) 231, 357
Hsp92II CATG 5 cut(s) 58, 142, 229, 512, 719
HspAI GCGC 2 cut(s) 159, 363
KroI GCCGGC 1 cut(s) 117
KroNI GCCGGC 1 cut(s) 119
Kzo9I GATC 3 cut(s) 91, 456, 529
LmnI GCTCC 5 cut(s) 214, 521, 549, 628, 677
Lsp1109I GCAGC 1 cut(s) 453
LweI GCATC 3 cut(s) 31, 88, 488
MaeI CTAG 1 cut(s) 552
MaeII ACGT 2 cut(s) 231, 357
MaeIII GTNAC 2 cut(s) 170, 571
MalI GATC 3 cut(s) 93, 458, 531
MboI GATC 3 cut(s) 91, 456, 529
MboII GAAGA 8 cut(s) 62, 154, 164, 208, 215, 588, 591, 594
MflI RGATCY 1 cut(s) 529
MluCI AATT 5 cut(s) 337, 407, 419, 489, 751
MlyI GAGTC 3 cut(s) 232, 506, 521
MmeI TCCRAC 2 cut(s) 499, 705
MnlI CCTC 6 cut(s) 205, 254, 435, 438, 652, 740
MroNI GCCGGC 1 cut(s) 117
MseI TTAA 4 cut(s) 300, 488, 492, 750
MslI CAYNNNNRTG 2 cut(s) 143, 230
MspI CCGG 2 cut(s) 118, 626
MspR9I CCNGG 2 cut(s) 38, 83
MvaI CCWGG 2 cut(s) 38, 83
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 1 cut(s) 327
NaeI GCCGGC 1 cut(s) 119
NdeI CATATG 1 cut(s) 165
NdeII GATC 3 cut(s) 91, 456, 529
NgoMIV GCCGGC 1 cut(s) 117
NlaIII CATG 5 cut(s) 58, 142, 229, 512, 719
NlaIV GGNNCC 2 cut(s) 630, 693
NsbI TGCGCA 1 cut(s) 160
NspI RCATGY 2 cut(s) 58, 719
PacI TTAATTAA 1 cut(s) 492
PciI ACATGT 1 cut(s) 715
PdiI GCCGGC 1 cut(s) 119
PfeI GAWTC 1 cut(s) 700
PflMI CCANNNNNTGG 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 81
PkrI GCNGC 3 cut(s) 367, 468, 471
PleI GAGTC 3 cut(s) 232, 505, 520
PpsI GAGTC 3 cut(s) 232, 505, 520
PscI ACATGT 1 cut(s) 715
PshBI ATTAAT 1 cut(s) 492
Psp1406I AACGTT 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 36, 81
PspGI CCWGG 2 cut(s) 36, 81
PspN4I GGNNCC 2 cut(s) 630, 693
PspPI GGNCC 1 cut(s) 106
PsuI RGATCY 1 cut(s) 529
RsaI GTAC 1 cut(s) 568
RsaNI GTAC 1 cut(s) 567
RseI CAYNNNNRTG 2 cut(s) 143, 230
SaqAI TTAA 4 cut(s) 300, 488, 492, 750
SatI GCNGC 3 cut(s) 366, 467, 470
Sau3AI GATC 3 cut(s) 91, 456, 529
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 3 cut(s) 232, 506, 521
ScrFI CCNGG 2 cut(s) 38, 83
SetI ASST 8 cut(s) 219, 234, 311, 360, 454, 468, 546, 674
SfaNI GCATC 3 cut(s) 31, 88, 488
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 2 cut(s) 143, 230
Sse9I AATT 5 cut(s) 337, 407, 419, 489, 751
SsiI CCGC 3 cut(s) 58, 365, 469
SspMI CTAG 1 cut(s) 552
StyD4I CCNGG 2 cut(s) 36, 81
TaaI ACNGT 1 cut(s) 571
TaiI ACGT 2 cut(s) 234, 360
TaqII GACCGA 1 cut(s) 123
TasI AATT 5 cut(s) 337, 407, 419, 489, 751
TatI WGTACW 1 cut(s) 566
TauI GCSGC 2 cut(s) 368, 472
TfiI GAWTC 1 cut(s) 700
Tru1I TTAA 4 cut(s) 300, 488, 492, 750
Tru9I TTAA 4 cut(s) 300, 488, 492, 750
TscAI CASTG 2 cut(s) 562, 660
TseI GCWGC 1 cut(s) 466
TspDTI ATGAA 2 cut(s) 155, 165
TspGWI ACGGA 1 cut(s) 505
TspRI CASTG 2 cut(s) 562, 660
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 106
VspI ATTAAT 1 cut(s) 492
XapI RAATTY 2 cut(s) 407, 419
XceI RCATGY 2 cut(s) 58, 719
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
XspI CTAG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.