Rh4BG062000

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000286
Physical Location & Seq
Forward (+)
11135144 .. 11136662
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG062000.1

Sequence Viewer

Length: 663 bp
ATGGCAATGGAAGAACTACATGCAGCTGCTTTAGCATACTACGACAATGGCACTCAACAGCTTCGGGATCTGGCAGGGTCTTTCTTCCGATCTATGGACACCAACGGGGATGGTCAAATCAGCTGCTTGGAGTTCAATGACTTCCTCCAGCAAAGTGGCTACAACTGGATTATTAACGATCCAAACTTCTTCAAAAGACTTGACCGCAATGGCGATGGTGGATTGGATTTTGGGGAAGTTCTCACTTTCTATTATATCATTAAAACGAGGTACATCAAGTGCCAAGGGTATCGGTGCGGTGTACACCTCTGTGGTTTGTATTTCACTTGCGTTGCTTGCTTTGACGGAGCTCATGAGCATCGCTCCACCTTTGATCTCTGTCCTGCCTGCTATCGCAATCGGAGTTACTATCACCACCAGCACCACACATACTTCTTGGATAATCATGTATTGCTGCGCTCCAAACGAGGCCTCTCTCCATATGCTCCACCAGATCTCAACATGGCAATGACTCCAACCCCGCCTCCAGCAGTTTATAATAATATTAATTACTACATCGCTGCTGCACCTCAAAGCGCGCGGCATAGTTGGTTCCAGGTATTTCGCGTGCTTGAGATGGCTCTTGCTGCTGCAAGTGTGGCTGCTAATTGTACAATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

220

Amino Acids

25.11

Weight (kDa)

6.53

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 537
AccII CGCG 3 cut(s) 578, 580, 606
AciI CCGC 4 cut(s) 205, 297, 521, 580
AclWI GGATC 2 cut(s) 75, 173
AfaI GTAC 3 cut(s) 272, 303, 652
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 2 cut(s) 136, 193
AjnI CCWGG 1 cut(s) 594
AleI CACNNNNGTG 1 cut(s) 309
AluBI AGCT 4 cut(s) 26, 61, 123, 350
AluI AGCT 4 cut(s) 26, 61, 123, 350
Alw21I GWGCWC 1 cut(s) 352
AlwI GGATC 2 cut(s) 75, 173
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 9 cut(s) 23, 26, 123, 454, 560, 563, 626, 629, 641
AseI ATTAAT 1 cut(s) 546
AspLEI GCGC 3 cut(s) 459, 578, 580
AsuHPI GGTGA 1 cut(s) 404
BanII GRGCYC 1 cut(s) 352
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 9 cut(s) 13, 35, 110, 441, 547, 550, 613, 616, 628
BccI CCATC 3 cut(s) 104, 209, 610
BciT130I CCWGG 1 cut(s) 596
BglII AGATCT 1 cut(s) 493
Bme1390I CCNGG 1 cut(s) 596
BmiI GGNNCC 1 cut(s) 593
BmrFI CCNGG 1 cut(s) 596
BmsI GCATC 1 cut(s) 367
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 2 cut(s) 131, 510
BpuEI CTTGAG 1 cut(s) 632
BsaJI CCNNGG 1 cut(s) 283
BsaXI ACNNNNNCTCC 2 cut(s) 508, 538
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 3 cut(s) 12, 214, 513
BseBI CCWGG 1 cut(s) 596
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 3 cut(s) 12, 214, 513
BseNI ACTGG 1 cut(s) 170
BsePI GCGCGC 1 cut(s) 576
BseXI GCAGC 9 cut(s) 13, 35, 110, 441, 547, 550, 613, 616, 628
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 3 cut(s) 578, 580, 606
BshFI GGCC 1 cut(s) 471
BsiHKAI GWGCWC 1 cut(s) 352
BslI CCNNNNNNNGG 1 cut(s) 94
BsnI GGCC 1 cut(s) 471
Bsp1286I GDGCHC 1 cut(s) 352
Bsp1407I TGTACA 2 cut(s) 301, 650
Bsp143I GATC 5 cut(s) 67, 89, 178, 373, 493
BspACI CCGC 4 cut(s) 205, 297, 521, 580
BspANI GGCC 1 cut(s) 471
BspFNI CGCG 3 cut(s) 578, 580, 606
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 1 cut(s) 593
BspPI GGATC 2 cut(s) 75, 173
BsrDI GCAATG 3 cut(s) 12, 214, 513
BsrGI TGTACA 2 cut(s) 301, 650
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 283
BssHII GCGCGC 1 cut(s) 576
BssMI GATC 5 cut(s) 67, 89, 178, 373, 493
BssT1I CCWWGG 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 596
BstAUI TGTACA 2 cut(s) 301, 650
BstC8I GCNNGC 4 cut(s) 337, 388, 578, 608
BstF5I GGATG 1 cut(s) 115
BstFNI CGCG 3 cut(s) 578, 580, 606
BstHHI GCGC 3 cut(s) 459, 578, 580
BstKTI GATC 5 cut(s) 70, 92, 181, 376, 496
BstMBI GATC 5 cut(s) 67, 89, 178, 373, 493
BstMWI GCNNNNNNNGC 4 cut(s) 32, 336, 626, 638
BstNI CCWGG 1 cut(s) 596
BstNSI RCATGY 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 594
BstUI CGCG 3 cut(s) 578, 580, 606
BstV1I GCAGC 9 cut(s) 13, 35, 110, 441, 547, 550, 613, 616, 628
BstX2I RGATCY 2 cut(s) 67, 493
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 2 cut(s) 67, 493
BsuRI GGCC 1 cut(s) 471
BtgZI GCGATG 3 cut(s) 228, 344, 541
BtsCI GGATG 1 cut(s) 115
Cac8I GCNNGC 4 cut(s) 337, 388, 578, 608
CciI TCATGA 1 cut(s) 352
CfoI GCGC 3 cut(s) 459, 578, 580
Csp6I GTAC 3 cut(s) 271, 302, 651
CviAII CATG 5 cut(s) 20, 353, 446, 502, 658
CviJI RGCY 8 cut(s) 26, 61, 123, 159, 350, 471, 620, 641
CviKI_1 RGCY 8 cut(s) 26, 61, 123, 159, 350, 471, 620, 641
CviQI GTAC 3 cut(s) 271, 302, 651
DpnI GATC 5 cut(s) 69, 91, 180, 375, 495
DpnII GATC 5 cut(s) 67, 89, 178, 373, 493
Ecl136II GAGCTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 283
Eco147I AGGCCT 1 cut(s) 471
Eco24I GRGCYC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 350
EcoICRI GAGCTC 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 594
EcoT14I CCWWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 5 cut(s) 23, 356, 449, 505, 661
FatI CATG 5 cut(s) 19, 352, 445, 501, 657
FauI CCCGC 1 cut(s) 528
FauNDI CATATG 1 cut(s) 481
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 352
GlaI GCGC 3 cut(s) 458, 577, 579
GsuI CTGGAG 2 cut(s) 131, 510
HaeIII GGCC 1 cut(s) 471
HhaI GCGC 3 cut(s) 459, 578, 580
Hin1II CATG 5 cut(s) 23, 356, 449, 505, 661
Hin6I GCGC 3 cut(s) 457, 576, 578
HinP1I GCGC 3 cut(s) 457, 576, 578
HinfI GANTC 1 cut(s) 511
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 2 cut(s) 302, 304
Hpy188I TCNGA 2 cut(s) 89, 402
Hpy188III TCNNGA 2 cut(s) 65, 353
Hpy8I GTNNAC 2 cut(s) 302, 304
HpyCH4V TGCA 3 cut(s) 23, 566, 632
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 336, 626, 638
Hsp92II CATG 5 cut(s) 23, 356, 449, 505, 661
HspAI GCGC 3 cut(s) 457, 576, 578
Kzo9I GATC 5 cut(s) 67, 89, 178, 373, 493
LmnI GCTCC 4 cut(s) 347, 368, 464, 490
Lsp1109I GCAGC 9 cut(s) 13, 35, 110, 441, 547, 550, 613, 616, 628
LweI GCATC 1 cut(s) 367
MaeIII GTNAC 1 cut(s) 404
MalI GATC 5 cut(s) 69, 91, 180, 375, 495
MboI GATC 5 cut(s) 67, 89, 178, 373, 493
MboII GAAGA 3 cut(s) 23, 76, 181
MflI RGATCY 2 cut(s) 67, 493
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 2 cut(s) 547, 646
MlyI GAGTC 1 cut(s) 505
MmeI TCCRAC 1 cut(s) 539
MnlI CCTC 7 cut(s) 155, 261, 317, 461, 482, 534, 579
MseI TTAA 3 cut(s) 174, 261, 546
MslI CAYNNNNRTG 2 cut(s) 309, 506
MspA1I CMGCKG 2 cut(s) 26, 123
MspR9I CCNGG 1 cut(s) 596
MvaI CCWGG 1 cut(s) 596
MvnI CGCG 3 cut(s) 578, 580, 606
MwoI GCNNNNNNNGC 4 cut(s) 32, 336, 626, 638
NdeI CATATG 1 cut(s) 481
NdeII GATC 5 cut(s) 67, 89, 178, 373, 493
NlaIII CATG 5 cut(s) 23, 356, 449, 505, 661
NlaIV GGNNCC 1 cut(s) 593
NspI RCATGY 1 cut(s) 23
OliI CACNNNNGTG 1 cut(s) 309
PagI TCATGA 1 cut(s) 352
PauI GCGCGC 1 cut(s) 576
PceI AGGCCT 1 cut(s) 471
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
PshBI ATTAAT 1 cut(s) 546
PsiI TTATAA 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 594
PspGI CCWGG 1 cut(s) 594
PspN4I GGNNCC 1 cut(s) 593
PsuI RGATCY 2 cut(s) 67, 493
PteI GCGCGC 1 cut(s) 576
PvuII CAGCTG 2 cut(s) 26, 123
RsaI GTAC 3 cut(s) 272, 303, 652
RsaNI GTAC 3 cut(s) 271, 302, 651
RseI CAYNNNNRTG 2 cut(s) 309, 506
SacI GAGCTC 1 cut(s) 352
SaqAI TTAA 3 cut(s) 174, 261, 546
Sau3AI GATC 5 cut(s) 67, 89, 178, 373, 493
SchI GAGTC 1 cut(s) 505
ScrFI CCNGG 1 cut(s) 596
SduI GDGCHC 1 cut(s) 352
SetI ASST 9 cut(s) 28, 63, 125, 272, 309, 352, 371, 571, 600
SfaNI GCATC 1 cut(s) 367
SmiMI CAYNNNNRTG 2 cut(s) 309, 506
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 2 cut(s) 547, 646
SseBI AGGCCT 1 cut(s) 471
SsiI CCGC 4 cut(s) 205, 297, 521, 580
SspI AATATT 1 cut(s) 544
SstI GAGCTC 1 cut(s) 352
StuI AGGCCT 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 594
StyI CCWWGG 1 cut(s) 283
TasI AATT 2 cut(s) 547, 646
TatI WGTACW 2 cut(s) 301, 650
TauI GCSGC 1 cut(s) 583
Tru1I TTAA 3 cut(s) 174, 261, 546
Tru9I TTAA 3 cut(s) 174, 261, 546
TseI GCWGC 9 cut(s) 23, 26, 123, 454, 560, 563, 626, 629, 641
TspGWI ACGGA 1 cut(s) 360
VspI ATTAAT 1 cut(s) 546
XceI RCATGY 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.