Rw4G004850

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000286
Physical Location & Seq
Reverse (-)
9515458 .. 9517745
2288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004850.1

Sequence Viewer

Length: 1152 bp
ATGGAAGAAATACGCGAAGTTGCTTTAGCATACTATGACAATTGCACACCAGAACTTCGGGGTAAGACATGGGAGTTCTTTCAATCCATGGATACGAACGGCGATCACCGAATCAGTGCCAGGGAGTTCAATGAATTCCTCCGTCAGACCGGTTACAACACGATCATTACTGACCCAGAATTATTTGCAAAGCTTGACCGCAACGGTGACTCCGGGCTGGATTTCGATGAAGTACTCACTTTCTACTATATCATTAAAACTGGGTACGTCTGGTGCCGACGCTGCAACAAGTACCTTTCCGGGCTCTATTTTACGTGCTTCGAATGCTTTGAGGCAGCCAATCCCTCCACTTTTGATCTATGTGATGCTTGTCATCGCAGTTGGAACTTTAAAGATCACCCCCACACACTCTTCGTGGAGAACCACATATTGCTATACTACAAAAGAGGACCAGATGACAGCATGGCACTGGAATATCTATATGCACAAGCTTTAGAGTACTATGATAAAGCAACTCCAGAGCTTAAGAGTTCAGTGATGTCTTTCTTTCAATCCATGGTTACACACGGTGCAAACCAAGTCAGCCTCTCTGTGTTCAACGATTTCCTCAAGCAAAAAGGCTACGACTGGATTTTTAGTGATCCACTCTTGTTCCAGAAACTCGACGGCAACCGTGATGGCGGCCTCGATTTCTGGGAATTGCTCACCTTCTACTACATCATCAACACAAGGTACTTCAATTGCCGAGAATGTCAGGTGTACCTTGGCGGGCTGTATTTTACCTGCGTTGCTTGCTTTGAGGGAGGAGCTCATTATCGTCGTCATCATCATTCTGATCCCGGCACCTATAATCTATGTGCTGCCTGCTATCGCAGTAGGAATTTCGTCGCTCAACACCCGCACCACACCTTTTTCTTGGATAACCACATATTGCTGCGCTCCAAAAGGGGTCTTCCTCCTTATGCTACACCAAGTCTGGTAAGCCCTCCAAAACCTCTGGTGGTTTATAATAATTATTATTTTATTAATAATAATTACCACCTTCCAGCTGTGCAGCCTGTACCTCCAAAGCAGAGTTGGCTATCGTCACTCAACCTATTAGAGGTGGCCCTGGCTGGTGCAAACTTGGCTGCTAATTGTTCGATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

383

Amino Acids

44.53

Weight (kDa)

6.0

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 26 - 83 4.4e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1008
Acc36I ACCTGC 1 cut(s) 791
AccB1I GGYRCC 2 cut(s) 273, 842
AccII CGCG 1 cut(s) 15
AciI CCGC 4 cut(s) 199, 681, 768, 899
AclWI GGATC 2 cut(s) 635, 830
AcsI RAATTY 2 cut(s) 134, 880
AdeI CACNNNGTG 1 cut(s) 569
AfaI GTAC 7 cut(s) 234, 266, 293, 500, 734, 761, 1062
AfiI CCNNNNNNNGG 1 cut(s) 1102
AflII CTTAAG 1 cut(s) 524
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 5 cut(s) 83, 130, 551, 598, 739
AjnI CCWGG 2 cut(s) 119, 1110
AluBI AGCT 5 cut(s) 193, 491, 523, 809, 1049
AluI AGCT 5 cut(s) 193, 491, 523, 809, 1049
Alw21I GWGCWC 1 cut(s) 811
AlwI GGATC 2 cut(s) 635, 830
AoxI GGCC 2 cut(s) 682, 1107
ApeKI GCWGC 6 cut(s) 282, 335, 860, 934, 1054, 1130
ApoI RAATTY 2 cut(s) 134, 880
AseI ATTAAT 1 cut(s) 1026
AsiGI ACCGGT 1 cut(s) 149
AspLEI GCGC 1 cut(s) 939
AspS9I GGNCC 2 cut(s) 449, 1108
AsuC2I CCSGG 3 cut(s) 214, 301, 840
AsuHPI GGTGA 4 cut(s) 98, 218, 389, 697
AsuII TTCGAA 1 cut(s) 321
AvaII GGWCC 1 cut(s) 449
BanI GGYRCC 2 cut(s) 273, 842
BanII GRGCYC 2 cut(s) 306, 811
BbsI GAAGAC 1 cut(s) 944
Bbv12I GWGCWC 1 cut(s) 811
BbvI GCAGC 6 cut(s) 269, 347, 847, 921, 1066, 1117
BccI CCATC 1 cut(s) 671
BceAI ACGGC 2 cut(s) 115, 682
BciT130I CCWGG 2 cut(s) 121, 1112
BciVI GTATCC 1 cut(s) 85
BcnI CCSGG 3 cut(s) 214, 301, 840
BfrI CTTAAG 1 cut(s) 524
BfuAI ACCTGC 1 cut(s) 791
BfuI GTATCC 1 cut(s) 85
BisI GCNGC 7 cut(s) 283, 336, 682, 861, 935, 1055, 1131
BlsI GCNGC 7 cut(s) 284, 337, 683, 862, 936, 1056, 1132
BmcAI AGTACT 2 cut(s) 234, 500
Bme1390I CCNGG 5 cut(s) 121, 214, 301, 840, 1112
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 2 cut(s) 449, 1108
BmiI GGNNCC 2 cut(s) 275, 844
BmrFI CCNGG 5 cut(s) 121, 214, 301, 840, 1112
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 355
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 944
BpmI CTGGAG 1 cut(s) 501
Bpu14I TTCGAA 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 593
BpuMI CCSGG 3 cut(s) 214, 301, 840
BsaAI YACGTR 1 cut(s) 315
BsaJI CCNNGG 5 cut(s) 87, 120, 555, 763, 1110
BsaWI WCCGGW 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 1102
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 3 cut(s) 265, 474, 632
BseBI CCWGG 2 cut(s) 121, 1112
BseDI CCNNGG 5 cut(s) 87, 120, 555, 763, 1110
BseLI CCNNNNNNNGG 1 cut(s) 1102
BseNI ACTGG 3 cut(s) 265, 474, 632
BseRI GAGGAG 1 cut(s) 819
BseXI GCAGC 6 cut(s) 269, 347, 847, 921, 1066, 1117
BsgI GTGCAG 1 cut(s) 1073
Bsh1236I CGCG 1 cut(s) 15
BshFI GGCC 2 cut(s) 684, 1109
BshNI GGYRCC 2 cut(s) 273, 842
BshTI ACCGGT 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 811
BsiSI CCGG 4 cut(s) 150, 213, 300, 840
BslI CCNNNNNNNGG 1 cut(s) 1102
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 2 cut(s) 684, 1109
Bsp119I TTCGAA 1 cut(s) 321
Bsp1286I GDGCHC 2 cut(s) 306, 811
Bsp143I GATC 6 cut(s) 103, 162, 355, 394, 640, 835
Bsp19I CCATGG 2 cut(s) 87, 555
BspACI CCGC 4 cut(s) 199, 681, 768, 899
BspANI GGCC 2 cut(s) 684, 1109
BspFNI CGCG 1 cut(s) 15
BspLI GGNNCC 2 cut(s) 275, 844
BspMI ACCTGC 1 cut(s) 791
BspPI GGATC 2 cut(s) 635, 830
BspT104I TTCGAA 1 cut(s) 321
BspT107I GGYRCC 2 cut(s) 273, 842
BspTI CTTAAG 1 cut(s) 524
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 3 cut(s) 265, 474, 632
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 5 cut(s) 87, 120, 555, 763, 1110
BssMI GATC 6 cut(s) 103, 162, 355, 394, 640, 835
BssT1I CCWWGG 3 cut(s) 87, 555, 763
Bst2UI CCWGG 2 cut(s) 121, 1112
Bst4CI ACNGT 3 cut(s) 206, 569, 674
Bst6I CTCTTC 1 cut(s) 416
BstAFI CTTAAG 1 cut(s) 524
BstBAI YACGTR 1 cut(s) 315
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 3 cut(s) 770, 793, 865
BstDSI CCRYGG 2 cut(s) 87, 555
BstENI CCTNNNNNAGG 1 cut(s) 1100
BstFNI CGCG 1 cut(s) 15
BstHHI GCGC 1 cut(s) 939
BstKTI GATC 6 cut(s) 106, 165, 358, 397, 643, 838
BstMBI GATC 6 cut(s) 103, 162, 355, 394, 640, 835
BstMWI GCNNNNNNNGC 5 cut(s) 282, 324, 792, 1078, 1127
BstNI CCWGG 2 cut(s) 121, 1112
BstSCI CCNGG 5 cut(s) 119, 212, 299, 838, 1110
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 6 cut(s) 269, 347, 847, 921, 1066, 1117
BstV2I GAAGAC 1 cut(s) 944
BsuI GTATCC 1 cut(s) 85
BsuRI GGCC 2 cut(s) 684, 1109
BtgI CCRYGG 2 cut(s) 87, 555
BtgZI GCGATG 1 cut(s) 359
BtsIMutI CAGTG 3 cut(s) 121, 467, 540
BveI ACCTGC 1 cut(s) 791
Cac8I GCNNGC 3 cut(s) 770, 793, 865
CfoI GCGC 1 cut(s) 939
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 449, 1108
CseI GACGC 1 cut(s) 288
Csp6I GTAC 7 cut(s) 233, 265, 292, 499, 733, 760, 1061
CspAI ACCGGT 1 cut(s) 149
CviAII CATG 4 cut(s) 69, 88, 463, 556
CviQI GTAC 7 cut(s) 233, 265, 292, 499, 733, 760, 1061
DpnI GATC 6 cut(s) 105, 164, 357, 396, 642, 837
DpnII GATC 6 cut(s) 103, 162, 355, 394, 640, 835
DraI TTTAAA 1 cut(s) 391
DraIII CACNNNGTG 1 cut(s) 569
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 809
Eco130I CCWWGG 3 cut(s) 87, 555, 763
Eco24I GRGCYC 2 cut(s) 306, 811
Eco47I GGWCC 1 cut(s) 449
Eco53kI GAGCTC 1 cut(s) 809
EcoICRI GAGCTC 1 cut(s) 809
EcoNI CCTNNNNNAGG 1 cut(s) 1100
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 119, 1110
EcoT14I CCWWGG 3 cut(s) 87, 555, 763
EcoT38I GRGCYC 2 cut(s) 306, 811
ErhI CCWWGG 3 cut(s) 87, 555, 763
FaeI CATG 4 cut(s) 72, 91, 466, 559
FatI CATG 4 cut(s) 68, 87, 462, 555
FauI CCCGC 2 cut(s) 761, 906
Fnu4HI GCNGC 7 cut(s) 283, 336, 682, 861, 935, 1055, 1131
FriOI GRGCYC 2 cut(s) 306, 811
Fsp4HI GCNGC 7 cut(s) 283, 336, 682, 861, 935, 1055, 1131
GlaI GCGC 1 cut(s) 938
GluI GCNGC 7 cut(s) 283, 336, 682, 861, 935, 1055, 1131
GsuI CTGGAG 1 cut(s) 501
HaeIII GGCC 2 cut(s) 684, 1109
HapII CCGG 4 cut(s) 150, 213, 300, 840
HgaI GACGC 1 cut(s) 288
HhaI GCGC 1 cut(s) 939
Hin1II CATG 4 cut(s) 72, 91, 466, 559
Hin6I GCGC 1 cut(s) 937
HinP1I GCGC 1 cut(s) 937
HindIII AAGCTT 2 cut(s) 191, 489
HinfI GANTC 2 cut(s) 111, 209
HpaII CCGG 4 cut(s) 150, 213, 300, 840
HphI GGTGA 4 cut(s) 98, 218, 389, 697
Hpy166II GTNNAC 1 cut(s) 760
Hpy188I TCNGA 2 cut(s) 147, 835
Hpy188III TCNNGA 2 cut(s) 518, 655
Hpy8I GTNNAC 1 cut(s) 760
Hpy99I CGWCG 4 cut(s) 282, 668, 822, 890
HpyAV CCTTC 2 cut(s) 718, 1052
HpyCH4III ACNGT 3 cut(s) 206, 569, 674
HpyCH4IV ACGT 2 cut(s) 267, 314
HpyCH4V TGCA 7 cut(s) 45, 188, 285, 485, 572, 1054, 1121
HpyF10VI GCNNNNNNNGC 5 cut(s) 282, 324, 792, 1078, 1127
HpySE526I ACGT 2 cut(s) 267, 314
Hsp92II CATG 4 cut(s) 72, 91, 466, 559
HspAI GCGC 1 cut(s) 937
Kzo9I GATC 6 cut(s) 103, 162, 355, 394, 640, 835
LmnI GCTCC 2 cut(s) 806, 944
Lsp1109I GCAGC 6 cut(s) 269, 347, 847, 921, 1066, 1117
LweI GCATC 1 cut(s) 355
MaeII ACGT 2 cut(s) 267, 314
MaeIII GTNAC 4 cut(s) 152, 206, 559, 1086
MalI GATC 6 cut(s) 105, 164, 357, 396, 642, 837
MboI GATC 6 cut(s) 103, 162, 355, 394, 640, 835
MboII GAAGA 3 cut(s) 17, 403, 944
MfeI CAATTG 2 cut(s) 40, 739
MhlI GDGCHC 2 cut(s) 306, 811
MluCI AATT 9 cut(s) 40, 134, 179, 698, 739, 880, 1012, 1033, 1135
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 362
MseI TTAA 4 cut(s) 255, 390, 525, 1026
MspA1I CMGCKG 1 cut(s) 1049
MspCI CTTAAG 1 cut(s) 524
MspI CCGG 4 cut(s) 150, 213, 300, 840
MspR9I CCNGG 5 cut(s) 121, 214, 301, 840, 1112
MunI CAATTG 2 cut(s) 40, 739
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 2 cut(s) 121, 1112
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 5 cut(s) 282, 324, 792, 1078, 1127
NciI CCSGG 3 cut(s) 214, 301, 840
NcoI CCATGG 2 cut(s) 87, 555
NdeII GATC 6 cut(s) 103, 162, 355, 394, 640, 835
NlaIII CATG 4 cut(s) 72, 91, 466, 559
NlaIV GGNNCC 2 cut(s) 275, 844
NmeAIII GCCGAG 1 cut(s) 770
NmuCI GTSAC 2 cut(s) 206, 1086
NspV TTCGAA 1 cut(s) 321
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 111
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 7 cut(s) 284, 337, 683, 862, 936, 1056, 1132
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
Ppu21I YACGTR 1 cut(s) 315
PshBI ATTAAT 1 cut(s) 1026
PsiI TTATAA 1 cut(s) 1008
Psp124BI GAGCTC 1 cut(s) 811
Psp6I CCWGG 2 cut(s) 119, 1110
PspGI CCWGG 2 cut(s) 119, 1110
PspN4I GGNNCC 2 cut(s) 275, 844
PspPI GGNCC 2 cut(s) 449, 1108
PvuII CAGCTG 1 cut(s) 1049
RsaI GTAC 7 cut(s) 234, 266, 293, 500, 734, 761, 1062
RsaNI GTAC 7 cut(s) 233, 265, 292, 499, 733, 760, 1061
SacI GAGCTC 1 cut(s) 811
SaqAI TTAA 4 cut(s) 255, 390, 525, 1026
SatI GCNGC 7 cut(s) 283, 336, 682, 861, 935, 1055, 1131
Sau3AI GATC 6 cut(s) 103, 162, 355, 394, 640, 835
Sau96I GGNCC 2 cut(s) 449, 1108
ScaI AGTACT 2 cut(s) 234, 500
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 5 cut(s) 121, 214, 301, 840, 1112
SduI GDGCHC 2 cut(s) 306, 811
SfaNI GCATC 1 cut(s) 355
SfuI TTCGAA 1 cut(s) 321
SinI GGWCC 1 cut(s) 449
SmlI CTYRAG 2 cut(s) 524, 608
SmoI CTYRAG 2 cut(s) 524, 608
Sse9I AATT 9 cut(s) 40, 134, 179, 698, 739, 880, 1012, 1033, 1135
SsiI CCGC 4 cut(s) 199, 681, 768, 899
SstI GAGCTC 1 cut(s) 811
StyD4I CCNGG 5 cut(s) 119, 212, 299, 838, 1110
StyI CCWWGG 3 cut(s) 87, 555, 763
TaaI ACNGT 3 cut(s) 206, 569, 674
TaiI ACGT 2 cut(s) 270, 317
TaqI TCGA 5 cut(s) 225, 321, 663, 687, 1142
TasI AATT 9 cut(s) 40, 134, 179, 698, 739, 880, 1012, 1033, 1135
TatI WGTACW 2 cut(s) 232, 498
TauI GCSGC 1 cut(s) 684
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 4 cut(s) 255, 390, 525, 1026
Tru9I TTAA 4 cut(s) 255, 390, 525, 1026
TscAI CASTG 3 cut(s) 121, 474, 540
TseFI GTSAC 2 cut(s) 206, 1086
TseI GCWGC 6 cut(s) 282, 335, 860, 934, 1054, 1130
Tsp45I GTSAC 2 cut(s) 206, 1086
TspDTI ATGAA 2 cut(s) 147, 243
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 3 cut(s) 121, 474, 540
Vha464I CTTAAG 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 449
VspI ATTAAT 1 cut(s) 1026
XagI CCTNNNNNAGG 1 cut(s) 1100
XapI RAATTY 2 cut(s) 134, 880
XcmI CCANNNNNNNNNTGG 1 cut(s) 1074
ZrmI AGTACT 2 cut(s) 234, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.