Rmu_sc0004598.1_g000029

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004598.1
OG0000286
Physical Location & Seq
Reverse (-)
110973 .. 111482
510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004598.1_g000029.1.cds

Sequence Viewer

Length: 510 bp
atgggagacatcaaagaaattcgtgatgctgctttagcttattaccaaaatttatctgcaaagcaacagcaagaggcgtacagcttttttcaaacaatggatacagatgatgatggaacaataaccatggaggagttcaagcagatgtttgagggtcattttggcgacaatatcaaccgtgccttccaggacctagacaaaaatggagatggcaatttggattttgaggaatatatcactctttactactctcttaaggctcagaggatcactttttgcgatggatgcaaagccttttttctaaagggactgtatttcacttgcgtggactgcttcaagcataaccaaactacaacttttgatctttgcacctcctgctaccgcatccgcaactacgttcacgaacactgcaactttttggacaactacgccttgctagctacttatagactttctggattgacaaaccaggtgatttatcaattgaactcttacagattagaagtctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

169

Amino Acids

19.84

Weight (kDa)

4.78

Isoelectric Point (pI)

21.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 382, 388
AclWI GGATC 1 cut(s) 275
AcsI RAATTY 2 cut(s) 18, 49
AfaI GTAC 1 cut(s) 80
AflII CTTAAG 1 cut(s) 254
AgsI TTSAA 4 cut(s) 92, 139, 337, 487
AjnI CCWGG 2 cut(s) 186, 468
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 3 cut(s) 38, 84, 440
AluI AGCT 3 cut(s) 38, 84, 440
AlwI GGATC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 2 cut(s) 18, 49
AspS9I GGNCC 1 cut(s) 190
AsuHPI GGTGA 1 cut(s) 484
AsuNHI GCTAGC 1 cut(s) 436
AvaII GGWCC 1 cut(s) 190
BbvI GCAGC 1 cut(s) 16
BccI CCATC 3 cut(s) 107, 203, 275
BciT130I CCWGG 2 cut(s) 188, 470
BciVI GTATCC 1 cut(s) 94
BfaI CTAG 2 cut(s) 194, 437
BfrI CTTAAG 1 cut(s) 254
BfuI GTATCC 1 cut(s) 94
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 2 cut(s) 188, 470
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 190
BmrFI CCNGG 2 cut(s) 188, 470
BmsI GCATC 3 cut(s) 16, 275, 393
BmtI GCTAGC 1 cut(s) 440
BsaJI CCNNGG 1 cut(s) 126
BseBI CCWGG 2 cut(s) 188, 470
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 2 cut(s) 290, 384
BseMII CTCAG 1 cut(s) 275
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 16
BslFI GGGAC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 321
Bsp143I GATC 2 cut(s) 267, 361
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 2 cut(s) 382, 388
BspCNI CTCAG 1 cut(s) 274
BspOI GCTAGC 1 cut(s) 440
BspPI GGATC 1 cut(s) 275
BspTI CTTAAG 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 2 cut(s) 267, 361
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 2 cut(s) 188, 470
Bst4CI ACNGT 2 cut(s) 179, 312
BstAFI CTTAAG 1 cut(s) 254
BstAPI GCANNNNNTGC 1 cut(s) 375
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 1 cut(s) 261
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 2 cut(s) 290, 384
BstKTI GATC 2 cut(s) 270, 364
BstMBI GATC 2 cut(s) 267, 361
BstMWI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 375, 437
BstNI CCWGG 2 cut(s) 188, 470
BstSCI CCNGG 2 cut(s) 186, 468
BstV1I GCAGC 1 cut(s) 16
BsuI GTATCC 1 cut(s) 94
BtgI CCRYGG 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 2 cut(s) 290, 384
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 1 cut(s) 438
Cfr13I GGNCC 1 cut(s) 190
CsiI ACCWGGT 1 cut(s) 468
Csp6I GTAC 1 cut(s) 79
CviAII CATG 1 cut(s) 127
CviJI RGCY 5 cut(s) 38, 84, 260, 293, 440
CviKI_1 RGCY 5 cut(s) 38, 84, 260, 293, 440
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 1 cut(s) 261
DpnI GATC 2 cut(s) 269, 363
DpnII GATC 2 cut(s) 267, 361
Eco130I CCWWGG 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 190
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 2 cut(s) 186, 468
EcoT14I CCWWGG 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 1 cut(s) 130
FaiI YATR 4 cut(s) 128, 234, 342, 447
FaqI GGGAC 1 cut(s) 321
FatI CATG 1 cut(s) 126
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 2 cut(s) 297, 371
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 2 cut(s) 194, 437
GluI GCNGC 1 cut(s) 30
Hin1II CATG 1 cut(s) 130
HphI GGTGA 1 cut(s) 484
Hpy166II GTNNAC 2 cut(s) 328, 400
Hpy188I TCNGA 1 cut(s) 264
Hpy188III TCNNGA 3 cut(s) 23, 401, 456
Hpy8I GTNNAC 2 cut(s) 328, 400
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 2 cut(s) 179, 312
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 4 cut(s) 59, 288, 369, 411
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 375, 437
HpyF3I CTNAG 1 cut(s) 261
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 1 cut(s) 130
Kzo9I GATC 2 cut(s) 267, 361
LpnPI CCDG 6 cut(s) 173, 200, 388, 441, 455, 482
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 16, 275, 393
MabI ACCWGGT 1 cut(s) 468
MaeI CTAG 2 cut(s) 194, 437
MaeII ACGT 1 cut(s) 396
MalI GATC 2 cut(s) 269, 363
MboI GATC 2 cut(s) 267, 361
MfeI CAATTG 1 cut(s) 482
MluCI AATT 4 cut(s) 18, 49, 214, 482
MnlI CCTC 6 cut(s) 67, 124, 145, 220, 258, 382
MseI TTAA 1 cut(s) 255
MslI CAYNNNNRTG 1 cut(s) 323
MspCI CTTAAG 1 cut(s) 254
MspR9I CCNGG 2 cut(s) 188, 470
MunI CAATTG 1 cut(s) 482
MvaI CCWGG 2 cut(s) 188, 470
MwoI GCNNNNNNNGC 5 cut(s) 35, 285, 330, 375, 437
NcoI CCATGG 1 cut(s) 126
NdeII GATC 2 cut(s) 267, 361
NheI GCTAGC 1 cut(s) 436
NlaIII CATG 1 cut(s) 130
OliI CACNNNNGTG 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 190
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 186, 468
PspGI CCWGG 2 cut(s) 186, 468
PspPI GGNCC 1 cut(s) 190
PspPPI RGGWCCY 1 cut(s) 190
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 2 cut(s) 267, 361
Sau96I GGNCC 1 cut(s) 190
ScrFI CCNGG 2 cut(s) 188, 470
SetI ASST 7 cut(s) 40, 86, 195, 374, 399, 442, 474
SexAI ACCWGGT 1 cut(s) 468
SfaNI GCATC 3 cut(s) 16, 275, 393
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 4 cut(s) 18, 49, 214, 482
SsiI CCGC 2 cut(s) 382, 388
SspMI CTAG 2 cut(s) 194, 437
StyD4I CCNGG 2 cut(s) 186, 468
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 179, 312
TaiI ACGT 1 cut(s) 399
TasI AATT 4 cut(s) 18, 49, 214, 482
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 1 cut(s) 413
TseI GCWGC 1 cut(s) 29
TspRI CASTG 1 cut(s) 413
Vha464I CTTAAG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 190
XapI RAATTY 2 cut(s) 18, 49
XspI CTAG 2 cut(s) 194, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.