RLG00000009595

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000286
Physical Location & Seq
Reverse (-)
55080649 .. 55082776
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009595

Sequence Viewer

Length: 1149 bp
ATGGAAGAAATACGTGAAGTAGCTTTAGCATACTATTACAATTGCACACCAGAACTTCAGGGTAAGACACGGGAATTCTTTCAATCCATGGATACGAACGGCGATCAGCGAATCTGTCCGAGGGAGTTCAATGAATTCCTCCGTCAGACCGGCTACAACACGATCATTACTGACCCAGAATTCTTTGCAAAGCTTGACCGCAACGGTGACCGCGGGCTGGATTTTGATGAAGTACTCACTTTCTACTATATCATTAAAACTGGGTATATCTGGTGCCGAGGCTGCAGCATGTACCTTTCCGGGCTCTATTTTACGTGCTTCGACTGCTTTGAGGAAGCCTGCAATCCCTCCACTTTTGATTTATGTGATACTTGCTATCGCAGTCGGAAGTTTACTCACCACCACACACGCTTCTTGGACAACCACACATTGCTATACTACAAAAGAGGACCAGATAACAACATGGCAATGGAATATCTATATGCAGAAGCTTTAGAGTACTATAATAAAGCAACTCCAGAGCTTCAGAGTTCAGTGGTGTCTTTCTTTCAATCCATGGTTACACACGGTGCAAACCAAATCAGCCGCACTGTGTTCAACGATTTCCTTAAGCAAAAAGGCTACAACTGGATTATTAATGATGGACACTTGTTCCAGAAACTCGACCGCAACGGTGATGGAAGCCTCGATTTCTGGGAACTGCTCAACTTCTACTACATCATCAGCACAAGGTACTTCCACTGCAGAGAATGTCAGGTGTACCTTAGCGGGCTGTATTTTACCTGCGTTGCTTGCTTTGAGGGAGGTGCTCATTATTGTCATCCCCATTATGATCCCGGCACCTATAATCTATGTGCTGCCTGCTATCGCAGTAGGAATTTCATCTCTCAACACCCTCACCACACATACTTCTTGGATAACCACATATTGAGACACTCCAAAAGAAGTCATCCTACTTATGCTGCACCATATCTGGTAAGCGCTCCAAAACCTCTGGTGGTTTATAATAATTATTATTATATTAATAATAATTACCTTCTTCCAGCTGTGCAGCCTGTACCTCCAAAGCAAACTTGGCTCTCGGCATTTAACCTATTAGAGGTGGCTCTGGCTGCTGCAAACTTGGCAGCTAATTGTTCAATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

383

Amino Acids

44.81

Weight (kDa)

6.23

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 24 - 83 1.2e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1005
Acc36I ACCTGC 1 cut(s) 791
AccB1I GGYRCC 2 cut(s) 273, 839
AccII CGCG 1 cut(s) 213
AciI CCGC 6 cut(s) 199, 211, 213, 586, 667, 768
AclWI GGATC 1 cut(s) 827
AcsI RAATTY 4 cut(s) 74, 134, 179, 877
AcuI CTGAAG 2 cut(s) 41, 509
AdeI CACNNNGTG 1 cut(s) 569
AfaI GTAC 6 cut(s) 234, 293, 500, 734, 761, 1059
AfeI AGCGCT 1 cut(s) 982
AfiI CCNNNNNNNGG 2 cut(s) 217, 1099
AflII CTTAAG 1 cut(s) 608
AgsI TTSAA 5 cut(s) 83, 130, 551, 598, 1140
AluBI AGCT 6 cut(s) 23, 193, 491, 523, 1046, 1130
AluI AGCT 6 cut(s) 23, 193, 491, 523, 1046, 1130
Alw21I GWGCWC 1 cut(s) 811
Alw26I GTCTC 1 cut(s) 925
AlwI GGATC 1 cut(s) 827
Aor51HI AGCGCT 1 cut(s) 982
ApeKI GCWGC 8 cut(s) 282, 285, 857, 962, 1051, 1112, 1115, 1127
ApoI RAATTY 4 cut(s) 74, 134, 179, 877
AseI ATTAAT 2 cut(s) 636, 1023
Asp700I GAANNNNTTC 1 cut(s) 78
AspLEI GCGC 1 cut(s) 983
AspS9I GGNCC 1 cut(s) 449
AsuC2I CCSGG 2 cut(s) 301, 837
AsuHPI GGTGA 4 cut(s) 218, 389, 686, 890
AvaII GGWCC 1 cut(s) 449
BanI GGYRCC 2 cut(s) 273, 839
BanII GRGCYC 1 cut(s) 306
BarI GAAGNNNNNNTAC 2 cut(s) 937, 969
Bbv12I GWGCWC 1 cut(s) 811
BbvI GCAGC 8 cut(s) 269, 297, 844, 949, 1063, 1099, 1102, 1139
BccI CCATC 2 cut(s) 635, 671
BceAI ACGGC 1 cut(s) 115
BciVI GTATCC 1 cut(s) 85
BcnI CCSGG 2 cut(s) 301, 837
BcoDI GTCTC 1 cut(s) 925
BfmI CTRYAG 2 cut(s) 283, 742
BfoI RGCGCY 1 cut(s) 984
BfrI CTTAAG 1 cut(s) 608
BfuAI ACCTGC 1 cut(s) 791
BfuI GTATCC 1 cut(s) 85
BisI GCNGC 9 cut(s) 283, 286, 586, 858, 963, 1052, 1113, 1116, 1128
BlsI GCNGC 9 cut(s) 284, 287, 587, 859, 964, 1053, 1114, 1117, 1129
BmcAI AGTACT 2 cut(s) 234, 500
Bme1390I CCNGG 2 cut(s) 301, 837
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 2 cut(s) 275, 841
BmrFI CCNGG 2 cut(s) 301, 837
BmrI ACTGGG 1 cut(s) 270
BmuI ACTGGG 1 cut(s) 270
BpmI CTGGAG 1 cut(s) 501
Bpu10I CCTNAGC 1 cut(s) 764
BpuMI CCSGG 2 cut(s) 301, 837
BsaAI YACGTR 2 cut(s) 14, 315
BsaJI CCNNGG 5 cut(s) 87, 119, 211, 277, 555
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 1099
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 2 cut(s) 265, 632
Bse3DI GCAATG 2 cut(s) 428, 474
BseDI CCNNGG 5 cut(s) 87, 119, 211, 277, 555
BseGI GGATG 2 cut(s) 820, 949
BseLI CCNNNNNNNGG 2 cut(s) 217, 1099
BseMI GCAATG 2 cut(s) 428, 474
BseNI ACTGG 2 cut(s) 265, 632
BseXI GCAGC 8 cut(s) 269, 297, 844, 949, 1063, 1099, 1102, 1139
BsgI GTGCAG 2 cut(s) 948, 1070
Bsh1236I CGCG 1 cut(s) 213
Bsh1285I CGRYCG 1 cut(s) 667
BshNI GGYRCC 2 cut(s) 273, 839
BsiEI CGRYCG 1 cut(s) 667
BsiHKAI GWGCWC 1 cut(s) 811
BsiSI CCGG 3 cut(s) 150, 300, 837
BslI CCNNNNNNNGG 2 cut(s) 217, 1099
BsmAI GTCTC 1 cut(s) 925
Bsp1286I GDGCHC 2 cut(s) 306, 811
Bsp143I GATC 3 cut(s) 103, 162, 832
Bsp19I CCATGG 2 cut(s) 87, 555
BspACI CCGC 6 cut(s) 199, 211, 213, 586, 667, 768
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 2 cut(s) 275, 841
BspMAI CTGCAG 2 cut(s) 287, 746
BspMI ACCTGC 1 cut(s) 791
BspPI GGATC 1 cut(s) 827
BspT107I GGYRCC 2 cut(s) 273, 839
BspTI CTTAAG 1 cut(s) 608
BsrDI GCAATG 2 cut(s) 428, 474
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 2 cut(s) 265, 632
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 5 cut(s) 87, 119, 211, 277, 555
BssMI GATC 3 cut(s) 103, 162, 832
BssT1I CCWWGG 2 cut(s) 87, 555
Bst4CI ACNGT 4 cut(s) 206, 569, 592, 674
BstAFI CTTAAG 1 cut(s) 608
BstBAI YACGTR 2 cut(s) 14, 315
BstC8I GCNNGC 5 cut(s) 215, 340, 770, 793, 862
BstDEI CTNAG 1 cut(s) 764
BstDSI CCRYGG 3 cut(s) 87, 211, 555
BstEII GGTNACC 1 cut(s) 206
BstENI CCTNNNNNAGG 1 cut(s) 1097
BstF5I GGATG 2 cut(s) 820, 949
BstFNI CGCG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 984
BstHHI GCGC 1 cut(s) 983
BstKTI GATC 3 cut(s) 106, 165, 835
BstMAI GTCTC 1 cut(s) 925
BstMBI GATC 3 cut(s) 103, 162, 832
BstMCI CGRYCG 1 cut(s) 667
BstMWI GCNNNNNNNGC 6 cut(s) 282, 324, 792, 1075, 1112, 1124
BstNSI RCATGY 1 cut(s) 292
BstPI GGTNACC 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 299, 835
BstSFI CTRYAG 2 cut(s) 283, 742
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 8 cut(s) 269, 297, 844, 949, 1063, 1099, 1102, 1139
BsuI GTATCC 1 cut(s) 85
BtgI CCRYGG 3 cut(s) 87, 211, 555
BtsCI GGATG 2 cut(s) 820, 949
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 3 cut(s) 540, 588, 739
BveI ACCTGC 1 cut(s) 791
Cac8I GCNNGC 5 cut(s) 215, 340, 770, 793, 862
CfoI GCGC 1 cut(s) 983
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 449
Cfr42I CCGCGG 1 cut(s) 214
Csp6I GTAC 6 cut(s) 233, 292, 499, 733, 760, 1058
CviAII CATG 4 cut(s) 88, 289, 463, 556
CviQI GTAC 6 cut(s) 233, 292, 499, 733, 760, 1058
DdeI CTNAG 1 cut(s) 764
DpnI GATC 3 cut(s) 105, 164, 834
DpnII GATC 3 cut(s) 103, 162, 832
DraIII CACNNNGTG 1 cut(s) 569
Eco130I CCWWGG 2 cut(s) 87, 555
Eco24I GRGCYC 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 449
Eco47III AGCGCT 1 cut(s) 982
Eco57I CTGAAG 2 cut(s) 41, 509
Eco91I GGTNACC 1 cut(s) 206
EcoNI CCTNNNNNAGG 1 cut(s) 1097
EcoO65I GGTNACC 1 cut(s) 206
EcoRI GAATTC 3 cut(s) 74, 134, 179
EcoT14I CCWWGG 2 cut(s) 87, 555
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 2 cut(s) 87, 555
FaeI CATG 4 cut(s) 91, 292, 466, 559
FatI CATG 4 cut(s) 87, 288, 462, 555
FauI CCCGC 2 cut(s) 206, 761
Fnu4HI GCNGC 9 cut(s) 283, 286, 586, 858, 963, 1052, 1113, 1116, 1128
FokI GGATG 2 cut(s) 807, 936
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 9 cut(s) 283, 286, 586, 858, 963, 1052, 1113, 1116, 1128
GlaI GCGC 1 cut(s) 982
GluI GCNGC 9 cut(s) 283, 286, 586, 858, 963, 1052, 1113, 1116, 1128
GsuI CTGGAG 1 cut(s) 501
HaeII RGCGCY 1 cut(s) 984
HapII CCGG 3 cut(s) 150, 300, 837
HhaI GCGC 1 cut(s) 983
Hin1II CATG 4 cut(s) 91, 292, 466, 559
Hin6I GCGC 1 cut(s) 981
HinP1I GCGC 1 cut(s) 981
HindIII AAGCTT 2 cut(s) 191, 489
HinfI GANTC 1 cut(s) 111
HpaII CCGG 3 cut(s) 150, 300, 837
HphI GGTGA 4 cut(s) 218, 389, 686, 890
Hpy166II GTNNAC 2 cut(s) 393, 760
Hpy188I TCNGA 4 cut(s) 120, 147, 387, 528
Hpy188III TCNNGA 2 cut(s) 518, 655
Hpy8I GTNNAC 2 cut(s) 393, 760
HpyAV CCTTC 1 cut(s) 1046
HpyCH4III ACNGT 4 cut(s) 206, 569, 592, 674
HpyCH4IV ACGT 2 cut(s) 13, 314
HpyF10VI GCNNNNNNNGC 6 cut(s) 282, 324, 792, 1075, 1112, 1124
HpyF3I CTNAG 1 cut(s) 764
HpySE526I ACGT 2 cut(s) 13, 314
Hsp92II CATG 4 cut(s) 91, 292, 466, 559
HspAI GCGC 1 cut(s) 981
KspI CCGCGG 1 cut(s) 214
Kzo9I GATC 3 cut(s) 103, 162, 832
LmnI GCTCC 1 cut(s) 988
Lsp1109I GCAGC 8 cut(s) 269, 297, 844, 949, 1063, 1099, 1102, 1139
MaeII ACGT 2 cut(s) 13, 314
MaeIII GTNAC 2 cut(s) 206, 559
MalI GATC 3 cut(s) 105, 164, 834
MboI GATC 3 cut(s) 103, 162, 832
MboII GAAGA 2 cut(s) 17, 1031
MfeI CAATTG 1 cut(s) 40
MhlI GDGCHC 2 cut(s) 306, 811
MluCI AATT 9 cut(s) 40, 74, 134, 179, 877, 1009, 1030, 1132, 1140
MmeI TCCRAC 1 cut(s) 365
MroXI GAANNNNTTC 1 cut(s) 78
MseI TTAA 5 cut(s) 255, 609, 636, 1023, 1089
MslI CAYNNNNRTG 1 cut(s) 467
MspA1I CMGCKG 2 cut(s) 213, 1046
MspCI CTTAAG 1 cut(s) 608
MspI CCGG 3 cut(s) 150, 300, 837
MspR9I CCNGG 2 cut(s) 301, 837
MunI CAATTG 1 cut(s) 40
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 6 cut(s) 282, 324, 792, 1075, 1112, 1124
NciI CCSGG 2 cut(s) 301, 837
NcoI CCATGG 2 cut(s) 87, 555
NdeII GATC 3 cut(s) 103, 162, 832
NlaIII CATG 4 cut(s) 91, 292, 466, 559
NlaIV GGNNCC 2 cut(s) 275, 841
NmeAIII GCCGAG 2 cut(s) 302, 1061
NmuCI GTSAC 1 cut(s) 206
NspI RCATGY 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 1 cut(s) 111
PkrI GCNGC 9 cut(s) 284, 287, 587, 859, 964, 1053, 1114, 1117, 1129
Ppu21I YACGTR 2 cut(s) 14, 315
PshBI ATTAAT 2 cut(s) 636, 1023
PsiI TTATAA 1 cut(s) 1005
PspEI GGTNACC 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 275, 841
PspPI GGNCC 1 cut(s) 449
PstI CTGCAG 2 cut(s) 287, 746
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 6 cut(s) 234, 293, 500, 734, 761, 1059
RsaNI GTAC 6 cut(s) 233, 292, 499, 733, 760, 1058
RseI CAYNNNNRTG 1 cut(s) 467
SacII CCGCGG 1 cut(s) 214
SaqAI TTAA 5 cut(s) 255, 609, 636, 1023, 1089
SatI GCNGC 9 cut(s) 283, 286, 586, 858, 963, 1052, 1113, 1116, 1128
Sau3AI GATC 3 cut(s) 103, 162, 832
Sau96I GGNCC 1 cut(s) 449
ScaI AGTACT 2 cut(s) 234, 500
ScrFI CCNGG 2 cut(s) 301, 837
SduI GDGCHC 2 cut(s) 306, 811
SfcI CTRYAG 2 cut(s) 283, 742
Sfr303I CCGCGG 1 cut(s) 214
SgrBI CCGCGG 1 cut(s) 214
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 1 cut(s) 467
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
Sse9I AATT 9 cut(s) 40, 74, 134, 179, 877, 1009, 1030, 1132, 1140
SsiI CCGC 6 cut(s) 199, 211, 213, 586, 667, 768
StyD4I CCNGG 2 cut(s) 299, 835
StyI CCWWGG 2 cut(s) 87, 555
TaaI ACNGT 4 cut(s) 206, 569, 592, 674
TaiI ACGT 2 cut(s) 16, 317
TaqI TCGA 3 cut(s) 321, 663, 687
TasI AATT 9 cut(s) 40, 74, 134, 179, 877, 1009, 1030, 1132, 1140
TatI WGTACW 2 cut(s) 232, 498
TauI GCSGC 1 cut(s) 588
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 5 cut(s) 255, 609, 636, 1023, 1089
Tru9I TTAA 5 cut(s) 255, 609, 636, 1023, 1089
TscAI CASTG 3 cut(s) 540, 595, 746
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 8 cut(s) 282, 285, 857, 962, 1051, 1112, 1115, 1127
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 3 cut(s) 147, 243, 871
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 3 cut(s) 540, 595, 746
Vha464I CTTAAG 1 cut(s) 608
VpaK11BI GGWCC 1 cut(s) 449
VspI ATTAAT 2 cut(s) 636, 1023
XagI CCTNNNNNAGG 1 cut(s) 1097
XapI RAATTY 4 cut(s) 74, 134, 179, 877
XceI RCATGY 1 cut(s) 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 1071
XmnI GAANNNNTTC 1 cut(s) 78
ZrmI AGTACT 2 cut(s) 234, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.