Rh5CG190000

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000286
Physical Location & Seq
Reverse (-)
18916500 .. 18916998
499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG190000.1

Sequence Viewer

Length: 499 bp
ATGGAGGAATTACTCCAGGCAGCAGTAGCATACTACAACAATGGTTCGAATAATCTCCGGGAACTAGCATCCAACTTCTTCAAGAGCATGGACACCAACCGTGACCGTCGAGTAAGCCGGTCGGAGTTCGTCCGATTTCTACAGCAAAGCGGGTACAATATCAACCCCTACATCTTCAACGCGCTCGATCGAAACGGCGACGGTTACCTGGACTTTTGGGAAGTTCTCACACTGTACTACATCATCAAAACCAGGTTGTATCATTGTGCTGACTGCGGAGTACGCCTCTTGGGGCTGTATTTCACGTGTGTTGAGTGCTTTGACAGAGCCGGCGATACCTATGATCTGTGCTCCATCTGCTATTCGGCCAGGCAGTTTCGTCACAGCCACACTCTCTTCTTGGATAATCATATGCTGCTCATAGCAAAAAGAAGACAAGCTCGTGGTAGTCATAACCTGAATCAGGTATTGCTTATTCTTTTTCTTTTTCTTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

166

Amino Acids

19.57

Weight (kDa)

8.22

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 26 - 80 1.8e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 182
AciI CCGC 2 cut(s) 150, 276
AcoI YGGCCR 1 cut(s) 366
AcvI CACGTG 1 cut(s) 306
AfaI GTAC 3 cut(s) 155, 236, 282
AfiI CCNNNNNNNGG 1 cut(s) 463
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 2 cut(s) 82, 178
AjnI CCWGG 4 cut(s) 15, 207, 251, 368
AluBI AGCT 1 cut(s) 440
AluI AGCT 1 cut(s) 440
Alw21I GWGCWC 1 cut(s) 353
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 2 cut(s) 20, 415
AspLEI GCGC 1 cut(s) 184
AsuC2I CCSGG 1 cut(s) 59
AsuII TTCGAA 1 cut(s) 47
BauI CACGAG 1 cut(s) 441
BbrPI CACGTG 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 439
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 2 cut(s) 32, 402
BccI CCATC 1 cut(s) 362
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 4 cut(s) 17, 209, 253, 370
BcnI CCSGG 1 cut(s) 59
BfaI CTAG 1 cut(s) 65
BfmI CTRYAG 1 cut(s) 140
BisI GCNGC 2 cut(s) 21, 416
BlsI GCNGC 2 cut(s) 22, 417
Bme1390I CCNGG 5 cut(s) 17, 59, 209, 253, 370
BmrFI CCNGG 5 cut(s) 17, 59, 209, 253, 370
BmsI GCATC 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 439
BplI GAGNNNNNCTC 2 cut(s) 270, 302
Bpu14I TTCGAA 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 59
BsaAI YACGTR 1 cut(s) 306
Bsc4I CCNNNNNNNGG 1 cut(s) 463
Bse118I RCCGGY 2 cut(s) 117, 329
BseBI CCWGG 4 cut(s) 17, 209, 253, 370
BseGI GGATG 1 cut(s) 68
BseLI CCNNNNNNNGG 1 cut(s) 463
BseXI GCAGC 2 cut(s) 32, 402
Bsh1236I CGCG 1 cut(s) 182
Bsh1285I CGRYCG 2 cut(s) 122, 190
BshFI GGCC 1 cut(s) 368
BsiEI CGRYCG 2 cut(s) 122, 190
BsiHKAI GWGCWC 1 cut(s) 353
BsiSI CCGG 3 cut(s) 58, 118, 330
BslI CCNNNNNNNGG 1 cut(s) 463
BsnI GGCC 1 cut(s) 368
Bsp119I TTCGAA 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 2 cut(s) 187, 343
BspACI CCGC 2 cut(s) 150, 276
BspANI GGCC 1 cut(s) 368
BspFNI CGCG 1 cut(s) 182
BspT104I TTCGAA 1 cut(s) 47
BsrFI RCCGGY 2 cut(s) 117, 329
BssAI RCCGGY 2 cut(s) 117, 329
BssMI GATC 2 cut(s) 187, 343
BssSI CACGAG 1 cut(s) 441
Bst2BI CACGAG 1 cut(s) 441
Bst2UI CCWGG 4 cut(s) 17, 209, 253, 370
Bst4CI ACNGT 4 cut(s) 101, 107, 203, 234
Bst6I CTCTTC 1 cut(s) 401
BstBAI YACGTR 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 331
BstEII GGTNACC 1 cut(s) 203
BstENI CCTNNNNNAGG 1 cut(s) 461
BstF5I GGATG 1 cut(s) 68
BstFNI CGCG 1 cut(s) 182
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 2 cut(s) 190, 346
BstMBI GATC 2 cut(s) 187, 343
BstMCI CGRYCG 2 cut(s) 122, 190
BstMWI GCNNNNNNNGC 3 cut(s) 26, 282, 357
BstNI CCWGG 4 cut(s) 17, 209, 253, 370
BstPI GGTNACC 1 cut(s) 203
BstSCI CCNGG 5 cut(s) 15, 57, 207, 251, 368
BstSFI CTRYAG 1 cut(s) 140
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 2 cut(s) 32, 402
BstV2I GAAGAC 1 cut(s) 439
BsuRI GGCC 1 cut(s) 368
BtsCI GGATG 1 cut(s) 68
BtsIMutI CAGTG 1 cut(s) 230
Cac8I GCNNGC 1 cut(s) 331
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 2 cut(s) 117, 329
CsiI ACCWGGT 1 cut(s) 251
Csp6I GTAC 3 cut(s) 154, 235, 281
CviAII CATG 1 cut(s) 88
CviJI RGCY 6 cut(s) 117, 295, 329, 368, 387, 440
CviKI_1 RGCY 6 cut(s) 117, 295, 329, 368, 387, 440
CviQI GTAC 3 cut(s) 154, 235, 281
DpnI GATC 2 cut(s) 189, 345
DpnII GATC 2 cut(s) 187, 343
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Eco72I CACGTG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 203
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoO65I GGTNACC 1 cut(s) 203
EcoRII CCWGG 4 cut(s) 15, 207, 251, 368
FaeI CATG 1 cut(s) 91
FaiI YATR 7 cut(s) 31, 89, 342, 411, 413, 422, 453
FatI CATG 1 cut(s) 87
FauI CCCGC 1 cut(s) 143
FauNDI CATATG 1 cut(s) 411
Fnu4HI GCNGC 2 cut(s) 21, 416
FokI GGATG 1 cut(s) 55
Fsp4HI GCNGC 2 cut(s) 21, 416
FspBI CTAG 1 cut(s) 65
GlaI GCGC 1 cut(s) 183
GluI GCNGC 2 cut(s) 21, 416
HaeIII GGCC 1 cut(s) 368
HapII CCGG 3 cut(s) 58, 118, 330
HhaI GCGC 1 cut(s) 184
Hin1II CATG 1 cut(s) 91
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HinfI GANTC 1 cut(s) 460
HpaII CCGG 3 cut(s) 58, 118, 330
Hpy188I TCNGA 2 cut(s) 124, 134
Hpy188III TCNNGA 1 cut(s) 82
Hpy99I CGWCG 2 cut(s) 111, 203
HpyCH4III ACNGT 4 cut(s) 101, 107, 203, 234
HpyCH4IV ACGT 1 cut(s) 305
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 282, 357
HpySE526I ACGT 1 cut(s) 305
Hsp92II CATG 1 cut(s) 91
HspAI GCGC 1 cut(s) 182
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Kzo9I GATC 2 cut(s) 187, 343
LmnI GCTCC 1 cut(s) 356
Lsp1109I GCAGC 2 cut(s) 32, 402
LweI GCATC 1 cut(s) 77
MabI ACCWGGT 1 cut(s) 251
MaeI CTAG 1 cut(s) 65
MaeII ACGT 1 cut(s) 305
MaeIII GTNAC 3 cut(s) 101, 203, 380
MalI GATC 2 cut(s) 189, 345
MboI GATC 2 cut(s) 187, 343
MboII GAAGA 4 cut(s) 70, 166, 388, 444
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 1 cut(s) 8
MmeI TCCRAC 2 cut(s) 96, 102
MnlI CCTC 1 cut(s) 296
MroNI GCCGGC 1 cut(s) 329
MseI TTAA 1 cut(s) 497
MspI CCGG 3 cut(s) 58, 118, 330
MspR9I CCNGG 5 cut(s) 17, 59, 209, 253, 370
MvaI CCWGG 4 cut(s) 17, 209, 253, 370
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 3 cut(s) 26, 282, 357
NaeI GCCGGC 1 cut(s) 331
NciI CCSGG 1 cut(s) 59
NdeI CATATG 1 cut(s) 411
NdeII GATC 2 cut(s) 187, 343
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 1 cut(s) 91
NmuCI GTSAC 2 cut(s) 101, 380
NspV TTCGAA 1 cut(s) 47
PdiI GCCGGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 460
PfoI TCCNGGA 1 cut(s) 57
PkrI GCNGC 2 cut(s) 22, 417
Ple19I CGATCG 1 cut(s) 190
PmaCI CACGTG 1 cut(s) 306
PmlI CACGTG 1 cut(s) 306
Ppu21I YACGTR 1 cut(s) 306
Psp6I CCWGG 4 cut(s) 15, 207, 251, 368
PspCI CACGTG 1 cut(s) 306
PspEI GGTNACC 1 cut(s) 203
PspGI CCWGG 4 cut(s) 15, 207, 251, 368
PvuI CGATCG 1 cut(s) 190
RsaI GTAC 3 cut(s) 155, 236, 282
RsaNI GTAC 3 cut(s) 154, 235, 281
SaqAI TTAA 1 cut(s) 497
SatI GCNGC 2 cut(s) 21, 416
Sau3AI GATC 2 cut(s) 187, 343
ScrFI CCNGG 5 cut(s) 17, 59, 209, 253, 370
SduI GDGCHC 1 cut(s) 353
SetI ASST 7 cut(s) 210, 257, 308, 341, 442, 459, 468
SexAI ACCWGGT 1 cut(s) 251
SfaNI GCATC 1 cut(s) 77
SfcI CTRYAG 1 cut(s) 140
SfuI TTCGAA 1 cut(s) 47
Sse9I AATT 1 cut(s) 8
SsiI CCGC 2 cut(s) 150, 276
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 5 cut(s) 15, 57, 207, 251, 368
TaaI ACNGT 4 cut(s) 101, 107, 203, 234
TaiI ACGT 1 cut(s) 308
TaqI TCGA 4 cut(s) 47, 109, 186, 190
TasI AATT 1 cut(s) 8
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 1 cut(s) 497
Tru9I TTAA 1 cut(s) 497
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 2 cut(s) 101, 380
TseI GCWGC 2 cut(s) 20, 415
Tsp45I GTSAC 2 cut(s) 101, 380
TspRI CASTG 1 cut(s) 237
XagI CCTNNNNNAGG 1 cut(s) 461
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.