Rorug03G0342500

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000286
Physical Location & Seq
Reverse (-)
41231480 .. 41234912
3433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0342500.1

Sequence Viewer

Length: 507 bp
ATGGGTTCTGCTGGTGCTCTGAATATGGCGTCGTCTTCCATTACTGTAATCAGCTCAACTTGTTCGTCAAAATCAAAGCTCAATGACAAATTCACCTGCGGGTTTCATAAATTCAATCCCATCACCAAAATCTGTGCCGTATCTCCAAACGGTTCCGTTTCTGGTTCTGCTTCTGCTTCACGCGGGGCTGAGTGCTCAGCTGGAGACGTGCATAAACAAAGAAGTAGTCTTGAATCTTTTTTTTGTTATGATAAGGCTATTCCGGAGGAGATTATTGAGAAGCCCATAGGGATATCTTTGGTGGAAAAATTCATTGGAAACAATCCCCGATGCATAGATTGTGAAGCAAAAGGCGCTGTCCTCTGCACAACTTGCTCTGGTTCAGGCCTCTATGTTGATTCAATATTGGAGAGCCAGGGCATTATTGTCAAAGTCCGCTGCCTAGGTTGTGGGGGAACTGGTAACATTATGTGTTCAGAGTGTGGTGGCCGCGGTCATTGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

168

Amino Acids

17.3

Weight (kDa)

7.91

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 104
AccII CGCG 2 cut(s) 183, 492
AccIII TCCGGA 1 cut(s) 262
AciI CCGC 5 cut(s) 99, 183, 436, 490, 492
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 3 cut(s) 89, 110, 308
AcyI GRCGYC 1 cut(s) 29
AgsI TTSAA 3 cut(s) 115, 233, 402
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 1 cut(s) 414
AjuI GAANNNNNNNTTGG 2 cut(s) 297, 329
AluBI AGCT 3 cut(s) 54, 79, 200
AluI AGCT 3 cut(s) 54, 79, 200
Alw21I GWGCWC 2 cut(s) 19, 197
Alw26I GTCTC 1 cut(s) 198
Aor13HI TCCGGA 1 cut(s) 262
AoxI GGCC 2 cut(s) 385, 487
ApeKI GCWGC 1 cut(s) 438
ApoI RAATTY 3 cut(s) 89, 110, 308
ArsI GACNNNNNNTTYG 2 cut(s) 211, 243
AspA2I CCTAGG 1 cut(s) 442
AspLEI GCGC 1 cut(s) 356
AsuHPI GGTGA 2 cut(s) 85, 115
AvrII CCTAGG 1 cut(s) 442
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 2 cut(s) 19, 197
BbvI GCAGC 1 cut(s) 425
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 198
BfaI CTAG 1 cut(s) 443
BfoI RGCGCY 1 cut(s) 357
BfuAI ACCTGC 1 cut(s) 104
BisI GCNGC 2 cut(s) 439, 490
BlnI CCTAGG 1 cut(s) 442
BlpI GCTNAGC 1 cut(s) 196
BlsI GCNGC 2 cut(s) 440, 491
Bme1390I CCNGG 1 cut(s) 416
BmgBI CACGTC 1 cut(s) 208
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 1 cut(s) 416
BmsI GCATC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 222
Bpu1102I GCTNAGC 1 cut(s) 196
BsaHI GRCGYC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 415, 442, 490
BsaWI WCCGGW 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 463
BseAI TCCGGA 1 cut(s) 262
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 3 cut(s) 415, 442, 490
BseMII CTCAG 2 cut(s) 180, 210
BseNI ACTGG 1 cut(s) 463
BseRI GAGGAG 1 cut(s) 281
BseXI GCAGC 1 cut(s) 425
BsgI GTGCAG 1 cut(s) 349
Bsh1236I CGCG 2 cut(s) 183, 492
BshFI GGCC 2 cut(s) 387, 489
BsiHKAI GWGCWC 2 cut(s) 19, 197
BsiSI CCGG 1 cut(s) 263
BsmAI GTCTC 1 cut(s) 198
BsmBI CGTCTC 1 cut(s) 198
BsnI GGCC 2 cut(s) 387, 489
Bsp1286I GDGCHC 2 cut(s) 19, 197
Bsp13I TCCGGA 1 cut(s) 262
Bsp1720I GCTNAGC 1 cut(s) 196
BspACI CCGC 5 cut(s) 99, 183, 436, 490, 492
BspANI GGCC 2 cut(s) 387, 489
BspCNI CTCAG 2 cut(s) 181, 209
BspEI TCCGGA 1 cut(s) 262
BspFNI CGCG 2 cut(s) 183, 492
BspLI GGNNCC 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 104
BsrI ACTGG 1 cut(s) 463
BssECI CCNNGG 3 cut(s) 415, 442, 490
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 2 cut(s) 46, 152
BstACI GRCGYC 1 cut(s) 29
BstAPI GCANNNNNTGC 1 cut(s) 372
BstDEI CTNAG 2 cut(s) 189, 196
BstDSI CCRYGG 1 cut(s) 490
BstFNI CGCG 2 cut(s) 183, 492
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 1 cut(s) 356
BstMAI GTCTC 1 cut(s) 198
BstMWI GCNNNNNNNGC 2 cut(s) 353, 372
BstNI CCWGG 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 414
BstUI CGCG 2 cut(s) 183, 492
BstV1I GCAGC 1 cut(s) 425
BstV2I GAAGAC 1 cut(s) 27
BsuRI GGCC 2 cut(s) 387, 489
BtgI CCRYGG 1 cut(s) 490
BtrI CACGTC 1 cut(s) 208
BveI ACCTGC 1 cut(s) 104
CfoI GCGC 1 cut(s) 356
Cfr42I CCGCGG 1 cut(s) 493
CseI GACGC 1 cut(s) 18
CviJI RGCY 9 cut(s) 54, 79, 188, 200, 257, 283, 387, 414, 489
CviKI_1 RGCY 9 cut(s) 54, 79, 188, 200, 257, 283, 387, 414, 489
DdeI CTNAG 2 cut(s) 189, 196
EaeI YGGCCR 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 442
Eco147I AGGCCT 1 cut(s) 387
Eco32I GATATC 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 414
EcoRV GATATC 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 442
Esp3I CGTCTC 1 cut(s) 198
FaiI YATR 8 cut(s) 26, 108, 213, 249, 287, 335, 393, 470
FauI CCCGC 2 cut(s) 92, 176
Fnu4HI GCNGC 2 cut(s) 439, 490
Fsp4HI GCNGC 2 cut(s) 439, 490
FspBI CTAG 1 cut(s) 443
GlaI GCGC 1 cut(s) 355
GluI GCNGC 2 cut(s) 439, 490
GsuI CTGGAG 1 cut(s) 222
HaeII RGCGCY 1 cut(s) 357
HaeIII GGCC 2 cut(s) 387, 489
HapII CCGG 1 cut(s) 263
HgaI GACGC 1 cut(s) 18
HhaI GCGC 1 cut(s) 356
Hin1I GRCGYC 1 cut(s) 29
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HinfI GANTC 2 cut(s) 233, 398
HpaII CCGG 1 cut(s) 263
HphI GGTGA 2 cut(s) 85, 115
Hpy188I TCNGA 2 cut(s) 21, 478
Hpy188III TCNNGA 2 cut(s) 230, 263
Hpy99I CGWCG 1 cut(s) 34
HpyCH4III ACNGT 2 cut(s) 46, 152
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 3 cut(s) 211, 333, 366
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 372
HpyF3I CTNAG 2 cut(s) 189, 196
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 29
HspAI GCGC 1 cut(s) 354
Kpn2I TCCGGA 1 cut(s) 262
KspI CCGCGG 1 cut(s) 493
LpnPI CCDG 9 cut(s) 109, 147, 186, 276, 363, 369, 401, 428, 444
Lsp1109I GCAGC 1 cut(s) 425
LweI GCATC 1 cut(s) 320
MaeI CTAG 1 cut(s) 443
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 1 cut(s) 461
MboII GAAGA 1 cut(s) 27
MhlI GDGCHC 2 cut(s) 19, 197
MluCI AATT 3 cut(s) 89, 110, 308
MnlI CCTC 3 cut(s) 259, 371, 398
Mph1103I ATGCAT 1 cut(s) 335
MroI TCCGGA 1 cut(s) 262
MspA1I CMGCKG 3 cut(s) 200, 438, 492
MspI CCGG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 416
MvaI CCWGG 1 cut(s) 416
MvnI CGCG 2 cut(s) 183, 492
MwoI GCNNNNNNNGC 2 cut(s) 353, 372
NlaIV GGNNCC 1 cut(s) 154
NsiI ATGCAT 1 cut(s) 335
PaqCI CACCTGC 1 cut(s) 104
PceI AGGCCT 1 cut(s) 387
PfeI GAWTC 2 cut(s) 233, 398
PkrI GCNGC 2 cut(s) 440, 491
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 154
PvuII CAGCTG 1 cut(s) 200
SacII CCGCGG 1 cut(s) 493
SatI GCNGC 2 cut(s) 439, 490
ScrFI CCNGG 1 cut(s) 416
SduI GDGCHC 2 cut(s) 19, 197
SetI ASST 6 cut(s) 56, 81, 98, 202, 210, 448
SfaNI GCATC 1 cut(s) 320
Sfr303I CCGCGG 1 cut(s) 493
SgrBI CCGCGG 1 cut(s) 493
Sse9I AATT 3 cut(s) 89, 110, 308
SseBI AGGCCT 1 cut(s) 387
SsiI CCGC 5 cut(s) 99, 183, 436, 490, 492
SspI AATATT 1 cut(s) 405
SspMI CTAG 1 cut(s) 443
StuI AGGCCT 1 cut(s) 387
StyD4I CCNGG 1 cut(s) 414
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 2 cut(s) 46, 152
TaiI ACGT 1 cut(s) 210
TasI AATT 3 cut(s) 89, 110, 308
TauI GCSGC 1 cut(s) 492
TfiI GAWTC 2 cut(s) 233, 398
TseI GCWGC 1 cut(s) 438
TspDTI ATGAA 2 cut(s) 95, 301
TspGWI ACGGA 1 cut(s) 145
XapI RAATTY 3 cut(s) 89, 110, 308
XmaJI CCTAGG 1 cut(s) 442
XspI CTAG 1 cut(s) 443
Zsp2I ATGCAT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.